Mutagenetix > Incidental Mutation

Incidental Mutation 'R9761:Or14j7'

733032

Institutional Source

Beutler Lab

Gene Symbol

Or14j7

Ensembl Gene

ENSMUSG00000059030

Gene Name

olfactory receptor family 14 subfamily J member 7

Synonyms

MOR218-13, GA_x6K02T2PSCP-2374126-2375048, Olfr128

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.069) question?

Stock #

R9761 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

38234459-38235385 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 38234934 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Serine at position 159 (F159S)

Ref Sequence

ENSEMBL: ENSMUSP00000151065 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000080231] [ENSMUST00000216440]

AlphaFold

Q7TRJ1

Predicted Effect

probably benign
Transcript: ENSMUST00000080231
AA Change: F159S

PolyPhen 2 Score 0.027 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000079121
Gene: ENSMUSG00000059030
AA Change: F159S

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.3e-46	PFAM
Pfam:7TM_GPCR_Srsx	35	305	1.1e-5	PFAM
Pfam:7tm_1	41	290	1.6e-21	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000216440
AA Change: F159S

PolyPhen 2 Score 0.027 (Sensitivity: 0.95; Specificity: 0.81)

Coding Region Coverage

1x: 99.9%
3x: 99.8%
10x: 99.4%
20x: 98.7%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc9	G	A	6: 142,544,854 (GRCm39)	T1411I	possibly damaging	Het
Ablim3	C	A	18: 61,952,885 (GRCm39)	R393L	possibly damaging	Het
Akap3	A	G	6: 126,842,200 (GRCm39)	D273G	probably benign	Het
Ankrd35	C	T	3: 96,591,733 (GRCm39)	T673I	possibly damaging	Het
Atp11b	A	T	3: 35,903,607 (GRCm39)	K1114*	probably null	Het
Atp11b	C	T	3: 35,903,621 (GRCm39)	P947L	probably benign	Het
Atp11b	G	T	3: 35,903,616 (GRCm39)	D1117Y	probably damaging	Het
Cfap74	C	A	4: 155,550,844 (GRCm39)	H243Q	unknown	Het
Dgkh	A	T	14: 78,889,163 (GRCm39)	S111R	probably damaging	Het
Dpp6	G	A	5: 27,869,743 (GRCm39)	R505K	probably benign	Het
Elp4	C	T	2: 105,624,904 (GRCm39)	G330S	probably damaging	Het
Fsip2	A	T	2: 82,821,994 (GRCm39)	N5909I	probably benign	Het
Gbp5	A	G	3: 142,213,518 (GRCm39)	D478G	probably damaging	Het
Gcn1	A	G	5: 115,729,064 (GRCm39)	M792V	probably benign	Het
Gpr152	T	C	19: 4,193,227 (GRCm39)	L256P	probably damaging	Het
Greb1	C	T	12: 16,751,275 (GRCm39)	M1006I	probably benign	Het
Gzf1	C	T	2: 148,530,011 (GRCm39)	S514L	probably damaging	Het
Helz	C	A	11: 107,560,874 (GRCm39)	S1312*	probably null	Het
Hmcn1	T	C	1: 150,868,625 (GRCm39)	I60V	probably damaging	Het
Ift81	A	C	5: 122,729,146 (GRCm39)	D395E	probably benign	Het
Itfg1	T	C	8: 86,563,031 (GRCm39)	N123S	probably benign	Het
Klk1b1	C	A	7: 43,618,739 (GRCm39)	H39N	possibly damaging	Het
Klk1b24	A	T	7: 43,839,779 (GRCm39)	N46I	possibly damaging	Het
Klrg2	C	A	6: 38,607,287 (GRCm39)	W241L	probably damaging	Het
Lhx9	T	C	1: 138,774,934 (GRCm39)	N3S	probably benign	Het
Lrrc2	A	G	9: 110,809,942 (GRCm39)	D326G	possibly damaging	Het
Mansc1	A	T	6: 134,587,004 (GRCm39)	L391Q	probably damaging	Het
Mcidas	G	A	13: 113,135,453 (GRCm39)	R292H	probably benign	Het
Ms4a19	T	C	19: 11,119,754 (GRCm39)	T59A	probably benign	Het
Myh13	A	G	11: 67,251,294 (GRCm39)	E1391G	probably damaging	Het
Or12d13	T	A	17: 37,648,057 (GRCm39)	Q22L	possibly damaging	Het
Or4f53	C	A	2: 111,087,606 (GRCm39)	L49I	probably benign	Het
Or4f53	T	G	2: 111,087,607 (GRCm39)	L49R	possibly damaging	Het
Or8g26	T	C	9: 39,096,146 (GRCm39)	I221T	probably damaging	Het
Or8k20	C	T	2: 86,106,150 (GRCm39)	R227Q	probably benign	Het
Pfpl	G	C	19: 12,406,297 (GRCm39)	E183Q	probably damaging	Het
Poc1b	C	T	10: 98,965,356 (GRCm39)	S95F	probably benign	Het
Pprc1	A	G	19: 46,049,998 (GRCm39)	I104V	unknown	Het
Prkar1b	C	A	5: 139,092,410 (GRCm39)	V136L	probably benign	Het
Scn2a	A	G	2: 65,566,030 (GRCm39)	I1351M	probably damaging	Het
Serpina3c	G	T	12: 104,118,089 (GRCm39)	A83D	probably damaging	Het
Slc23a3	ATT	ATTT	1: 75,109,925 (GRCm39)		probably null	Het
Syne1	A	G	10: 5,318,190 (GRCm39)		probably null	Het
Ticrr	A	G	7: 79,345,313 (GRCm39)	E1726G	probably damaging	Het
Tmprss12	T	C	15: 100,190,529 (GRCm39)	W300R	probably damaging	Het
Tnrc6c	T	A	11: 117,623,136 (GRCm39)	H892Q	probably benign	Het
Tnxb	A	G	17: 34,903,987 (GRCm39)	T1043A	probably benign	Het
Vps50	T	A	6: 3,519,218 (GRCm39)	V100D	probably damaging	Het
Zim1	G	C	7: 6,680,771 (GRCm39)	H297Q	probably damaging	Het

Other mutations in Or14j7

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01922:Or14j7	APN	17	38,234,850 (GRCm39)	missense	possibly damaging	0.73
IGL01940:Or14j7	APN	17	38,235,177 (GRCm39)	missense	probably damaging	1.00
R0427:Or14j7	UTSW	17	38,234,520 (GRCm39)	missense	probably benign	0.00
R0462:Or14j7	UTSW	17	38,234,667 (GRCm39)	missense	probably damaging	1.00
R0546:Or14j7	UTSW	17	38,235,229 (GRCm39)	nonsense	probably null
R0760:Or14j7	UTSW	17	38,235,005 (GRCm39)	nonsense	probably null
R0940:Or14j7	UTSW	17	38,234,591 (GRCm39)	missense	probably damaging	0.98
R1898:Or14j7	UTSW	17	38,234,516 (GRCm39)	missense	possibly damaging	0.91
R1935:Or14j7	UTSW	17	38,234,993 (GRCm39)	missense	probably damaging	1.00
R2060:Or14j7	UTSW	17	38,234,771 (GRCm39)	missense	probably benign	0.00
R4680:Or14j7	UTSW	17	38,234,813 (GRCm39)	missense	probably damaging	1.00
R5165:Or14j7	UTSW	17	38,235,252 (GRCm39)	missense	probably benign	0.14
R5526:Or14j7	UTSW	17	38,235,383 (GRCm39)	makesense	probably null
R5594:Or14j7	UTSW	17	38,234,502 (GRCm39)	missense	probably benign	0.00
R5751:Or14j7	UTSW	17	38,234,861 (GRCm39)	missense	probably benign	0.44
R6330:Or14j7	UTSW	17	38,234,685 (GRCm39)	missense	possibly damaging	0.77
R7123:Or14j7	UTSW	17	38,234,567 (GRCm39)	missense	probably benign	0.33
R7354:Or14j7	UTSW	17	38,235,284 (GRCm39)	missense	probably benign	0.00
R7827:Or14j7	UTSW	17	38,234,568 (GRCm39)	nonsense	probably null
R8258:Or14j7	UTSW	17	38,234,847 (GRCm39)	missense	probably damaging	1.00
R8259:Or14j7	UTSW	17	38,234,847 (GRCm39)	missense	probably damaging	1.00
R8686:Or14j7	UTSW	17	38,235,168 (GRCm39)	missense	probably benign	0.00
R8787:Or14j7	UTSW	17	38,235,075 (GRCm39)	missense	probably benign	0.06
R9036:Or14j7	UTSW	17	38,235,168 (GRCm39)	missense	probably benign	0.03
R9278:Or14j7	UTSW	17	38,235,275 (GRCm39)	missense	probably benign
Z1177:Or14j7	UTSW	17	38,234,618 (GRCm39)	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- CGAAGTGGCTACATTTCATATGGG -3'
(R):5'- ATTTGGCCATAGGAGAAGGC -3'

Sequencing Primer
(F):5'- CTACATTTCATATGGGCAGTGCATGC -3'
(R):5'- TTGGCCATAGGAGAAGGCAATCC -3'

Posted On

2022-11-14