Incidental Mutation 'R9762:Cstf3'
ID 733042
Institutional Source Beutler Lab
Gene Symbol Cstf3
Ensembl Gene ENSMUSG00000027176
Gene Name cleavage stimulation factor, 3' pre-RNA, subunit 3
Synonyms 4732468G05Rik
MMRRC Submission
Accession Numbers
Essential gene? Probably essential (E-score: 0.966) question?
Stock # R9762 (G1)
Quality Score 225.009
Status Not validated
Chromosome 2
Chromosomal Location 104420868-104495774 bp(+) (GRCm39)
Type of Mutation nonsense
DNA Base Change (assembly) A to T at 104494684 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Arginine to Stop codon at position 645 (R645*)
Ref Sequence ENSEMBL: ENSMUSP00000028599 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000028599]
AlphaFold Q99LI7
PDB Structure Crystal Structure of the HAT-C domain of murine CstF-77 [X-RAY DIFFRACTION]
Crystal structure of HAT domain of murine CstF-77 [X-RAY DIFFRACTION]
Predicted Effect probably null
Transcript: ENSMUST00000028599
AA Change: R645*
SMART Domains Protein: ENSMUSP00000028599
Gene: ENSMUSG00000027176
AA Change: R645*

DomainStartEndE-ValueType
low complexity region 10 27 N/A INTRINSIC
HAT 45 77 4.67e-8 SMART
HAT 79 110 5.04e-4 SMART
HAT 117 152 1.38e-1 SMART
HAT 163 196 4.39e-4 SMART
HAT 229 261 1.19e0 SMART
HAT 271 303 9.12e0 SMART
HAT 319 352 2.73e0 SMART
HAT 354 387 7.31e-1 SMART
HAT 424 456 2.37e0 SMART
HAT 458 494 1.46e0 SMART
low complexity region 597 619 N/A INTRINSIC
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.8%
  • 10x: 99.4%
  • 20x: 98.6%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is one of three (including CSTF1 and CSTF2) cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in the polyadenylation and 3' end cleavage of pre-mRNAs. The encoded protein functions as a homodimer and interacts directly with both CSTF1 and CSTF2 in the CSTF complex. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 81 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Ablim1 G T 19: 57,025,691 (GRCm39) R851S probably damaging Het
Acap2 C A 16: 30,929,763 (GRCm39) A407S probably damaging Het
Adam26a A T 8: 44,021,635 (GRCm39) S618R probably benign Het
Adamtsl5 T C 10: 80,180,896 (GRCm39) Y89C probably damaging Het
Aebp2 T A 6: 140,588,021 (GRCm39) S364T probably damaging Het
Ankfy1 A G 11: 72,621,401 (GRCm39) E229G probably benign Het
Arhgap27 G T 11: 103,251,511 (GRCm39) D72E probably benign Het
Arhgap33 T C 7: 30,230,950 (GRCm39) E191G probably null Het
Atp6v0a1 C T 11: 100,946,427 (GRCm39) H807Y possibly damaging Het
Ccdc113 A C 8: 96,272,605 (GRCm39) E237D probably benign Het
Cd200r2 T A 16: 44,729,420 (GRCm39) I25K probably benign Het
Cd300c2 A G 11: 114,887,775 (GRCm39) V209A probably damaging Het
Cdkn2d T A 9: 21,200,383 (GRCm39) E129D probably benign Het
Cfap251 A G 5: 123,460,533 (GRCm39) Y1165C probably damaging Het
Chd9 A T 8: 91,712,741 (GRCm39) N855Y unknown Het
Cntn3 A G 6: 102,254,196 (GRCm39) C249R probably damaging Het
Col12a1 T C 9: 79,527,266 (GRCm39) E2688G possibly damaging Het
Copb2 C T 9: 98,464,901 (GRCm39) T612I probably benign Het
Cplx1 A T 5: 108,673,378 (GRCm39) D29E possibly damaging Het
Cracd A G 5: 77,006,555 (GRCm39) K972R unknown Het
Ctdp1 A T 18: 80,492,550 (GRCm39) Y648* probably null Het
Daam1 T A 12: 71,990,855 (GRCm39) D156E unknown Het
Dbn1 G A 13: 55,622,824 (GRCm39) P599L probably damaging Het
Diaph1 C A 18: 37,987,589 (GRCm39) V1056F probably damaging Het
Dzip3 A T 16: 48,748,707 (GRCm39) D870E probably damaging Het
Efcab12 T C 6: 115,800,331 (GRCm39) T231A possibly damaging Het
Efl1 A C 7: 82,412,596 (GRCm39) D995A probably benign Het
Fam186a T C 15: 99,842,393 (GRCm39) T1284A possibly damaging Het
Fbxo33 T C 12: 59,251,708 (GRCm39) E269G probably benign Het
Foxj3 T A 4: 119,483,540 (GRCm39) M604K unknown Het
Gabra4 A G 5: 71,814,463 (GRCm39) S86P unknown Het
Gnas G A 2: 174,140,639 (GRCm39) W269* probably null Het
Gpc6 T G 14: 118,202,258 (GRCm39) N489K probably damaging Het
Gprin3 T C 6: 59,331,236 (GRCm39) K357R possibly damaging Het
Grip1 G T 10: 119,811,906 (GRCm39) A286S possibly damaging Het
Grm4 A T 17: 27,721,688 (GRCm39) V151E probably damaging Het
Gucy1b1 A T 3: 81,942,065 (GRCm39) S565T possibly damaging Het
Hace1 T C 10: 45,525,014 (GRCm39) V260A probably benign Het
Hepacam2 A G 6: 3,486,940 (GRCm39) I139T probably damaging Het
Hspa4l T C 3: 40,727,057 (GRCm39) I486T probably benign Het
Igkv8-19 T C 6: 70,317,866 (GRCm39) Y120C probably damaging Het
Iglv2 C T 16: 19,079,548 (GRCm39) probably benign Het
Igsf10 A T 3: 59,237,106 (GRCm39) L1025H probably damaging Het
Itpkb A G 1: 180,161,752 (GRCm39) N626S probably benign Het
Jarid2 C T 13: 45,068,253 (GRCm39) R1092W possibly damaging Het
Klc3 C T 7: 19,132,023 (GRCm39) W118* probably null Het
Klhl25 C T 7: 75,516,741 (GRCm39) T549I probably damaging Het
Krtap6-2 T A 16: 89,216,763 (GRCm39) Y68F unknown Het
Mtcl1 T C 17: 66,673,347 (GRCm39) N1121D probably benign Het
Ncbp3 A G 11: 72,961,668 (GRCm39) K314R probably benign Het
Nop2 T C 6: 125,121,272 (GRCm39) W685R probably benign Het
Orc1 T A 4: 108,447,874 (GRCm39) D40E probably benign Het
Pappa2 G T 1: 158,684,948 (GRCm39) N730K probably damaging Het
Parpbp A G 10: 87,960,815 (GRCm39) S224P possibly damaging Het
Pdgfc A G 3: 80,944,792 (GRCm39) R17G probably benign Het
Pfpl G C 19: 12,406,297 (GRCm39) E183Q probably damaging Het
Pik3r6 T A 11: 68,424,358 (GRCm39) L321* probably null Het
Pnp2 T C 14: 51,197,006 (GRCm39) W31R probably damaging Het
Prr12 T C 7: 44,696,954 (GRCm39) Y987C unknown Het
Prr27 C A 5: 87,990,994 (GRCm39) P202Q probably benign Het
Prss23 T C 7: 89,159,683 (GRCm39) I129V probably damaging Het
Rhno1 T C 6: 128,336,119 (GRCm39) T39A probably damaging Het
Rps2 A T 17: 24,940,810 (GRCm39) R279* probably null Het
Ski T C 4: 155,244,344 (GRCm39) K427R probably damaging Het
Slc23a3 ATT ATTT 1: 75,109,925 (GRCm39) probably null Het
Slc26a7 C T 4: 14,546,372 (GRCm39) G319D probably damaging Het
Slc7a6os A G 8: 106,937,523 (GRCm39) V8A probably damaging Het
Smim8 A C 4: 34,771,265 (GRCm39) I43S probably damaging Het
Sox5 T C 6: 143,819,842 (GRCm39) K400R probably damaging Het
Tab1 G A 15: 80,032,943 (GRCm39) V76M probably damaging Het
Taf2 A C 15: 54,894,440 (GRCm39) probably null Het
Taf5 G A 19: 47,059,434 (GRCm39) V193M probably damaging Het
Tm2d1 A T 4: 98,246,261 (GRCm39) S180R probably damaging Het
Ttc12 A G 9: 49,368,166 (GRCm39) F287S probably damaging Het
Ubqln4 G A 3: 88,473,185 (GRCm39) probably null Het
Ubr3 T C 2: 69,839,497 (GRCm39) V1537A probably benign Het
Ugt8a A G 3: 125,708,900 (GRCm39) L70P probably damaging Het
Vps26a A T 10: 62,316,433 (GRCm39) N51K probably benign Het
Xkr5 T A 8: 18,990,749 (GRCm39) T173S probably benign Het
Zfp354c A G 11: 50,706,239 (GRCm39) C279R probably damaging Het
Zfp65 T A 13: 67,856,478 (GRCm39) H267L probably damaging Het
Other mutations in Cstf3
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01911:Cstf3 APN 2 104,476,976 (GRCm39) missense probably damaging 1.00
IGL02734:Cstf3 APN 2 104,439,539 (GRCm39) splice site probably benign
IGL03025:Cstf3 APN 2 104,439,276 (GRCm39) missense possibly damaging 0.82
Amanita UTSW 2 104,420,926 (GRCm39) start gained probably benign
Ptomaine UTSW 2 104,479,807 (GRCm39) missense probably benign
R0043:Cstf3 UTSW 2 104,475,430 (GRCm39) splice site probably benign
R0189:Cstf3 UTSW 2 104,482,791 (GRCm39) missense probably damaging 1.00
R0332:Cstf3 UTSW 2 104,476,812 (GRCm39) critical splice donor site probably null
R0499:Cstf3 UTSW 2 104,479,950 (GRCm39) missense possibly damaging 0.95
R1476:Cstf3 UTSW 2 104,478,564 (GRCm39) missense possibly damaging 0.82
R1677:Cstf3 UTSW 2 104,494,623 (GRCm39) intron probably benign
R1881:Cstf3 UTSW 2 104,484,563 (GRCm39) missense probably benign
R1916:Cstf3 UTSW 2 104,486,101 (GRCm39) missense possibly damaging 0.90
R3720:Cstf3 UTSW 2 104,483,431 (GRCm39) splice site probably benign
R3813:Cstf3 UTSW 2 104,439,466 (GRCm39) missense probably damaging 1.00
R5155:Cstf3 UTSW 2 104,482,830 (GRCm39) missense probably benign
R5304:Cstf3 UTSW 2 104,493,735 (GRCm39) nonsense probably null
R5564:Cstf3 UTSW 2 104,439,347 (GRCm39) intron probably benign
R5869:Cstf3 UTSW 2 104,489,585 (GRCm39) splice site probably null
R6172:Cstf3 UTSW 2 104,481,987 (GRCm39) missense probably damaging 1.00
R6747:Cstf3 UTSW 2 104,477,112 (GRCm39) missense probably damaging 1.00
R6841:Cstf3 UTSW 2 104,486,076 (GRCm39) missense probably benign 0.22
R6959:Cstf3 UTSW 2 104,479,807 (GRCm39) missense probably benign
R7139:Cstf3 UTSW 2 104,483,409 (GRCm39) missense possibly damaging 0.87
R7143:Cstf3 UTSW 2 104,476,961 (GRCm39) missense probably benign 0.01
R7350:Cstf3 UTSW 2 104,439,301 (GRCm39) missense probably damaging 1.00
R7794:Cstf3 UTSW 2 104,420,926 (GRCm39) start gained probably benign
R8315:Cstf3 UTSW 2 104,420,926 (GRCm39) start gained probably benign
R8873:Cstf3 UTSW 2 104,475,355 (GRCm39) missense possibly damaging 0.92
R9188:Cstf3 UTSW 2 104,439,223 (GRCm39) missense possibly damaging 0.68
R9339:Cstf3 UTSW 2 104,493,778 (GRCm39) missense probably damaging 1.00
R9612:Cstf3 UTSW 2 104,483,370 (GRCm39) missense possibly damaging 0.80
R9801:Cstf3 UTSW 2 104,421,024 (GRCm39) missense possibly damaging 0.73
X0013:Cstf3 UTSW 2 104,489,622 (GRCm39) missense possibly damaging 0.58
Predicted Primers PCR Primer
(F):5'- GCCGCTGTTGTTTTGATGAAAC -3'
(R):5'- GAGTATATGCCCACACTGCAG -3'

Sequencing Primer
(F):5'- GTTTTGATGAAACTGCTCCCAC -3'
(R):5'- TGCAGAAGGCCCAGCTTACTC -3'
Posted On 2022-11-14