Mutagenetix > Incidental Mutation

Incidental Mutation 'R9764:Zfp74'

733144

Institutional Source

Beutler Lab

Gene Symbol

Zfp74

Ensembl Gene

ENSMUSG00000059975

Gene Name

zinc finger protein 74

Synonyms

KRAB8, 2810054M15Rik, Zfp66

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.081) question?

Stock #

R9764 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

29632086-29653579 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 29631845 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Tyrosine at position 133 (F133Y)

Ref Sequence

ENSEMBL: ENSMUSP00000103847 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000032797] [ENSMUST00000108205] [ENSMUST00000108211] [ENSMUST00000108212]

AlphaFold

Q80W31

Predicted Effect

probably benign
Transcript: ENSMUST00000032797

SMART Domains

Protein: ENSMUSP00000032797
Gene: ENSMUSG00000059975

Domain	Start	End	E-Value	Type
KRAB	8	68	5.02e-38	SMART
ZnF_C2H2	179	201	1.84e-4	SMART
ZnF_C2H2	207	229	2.36e-2	SMART
ZnF_C2H2	235	257	1.5e-4	SMART
ZnF_C2H2	263	285	2.79e-4	SMART
ZnF_C2H2	291	313	3.49e-5	SMART
ZnF_C2H2	319	341	6.42e-4	SMART
ZnF_C2H2	347	369	5.59e-4	SMART
ZnF_C2H2	375	397	5.21e-4	SMART
ZnF_C2H2	403	425	3.63e-3	SMART
ZnF_C2H2	431	453	1.82e-3	SMART
ZnF_C2H2	459	481	1.58e-3	SMART
ZnF_C2H2	487	509	2.12e-4	SMART
ZnF_C2H2	515	537	1.82e-3	SMART
ZnF_C2H2	543	565	2.24e-3	SMART
ZnF_C2H2	571	593	8.02e-5	SMART
ZnF_C2H2	599	621	1.04e-3	SMART
ZnF_C2H2	627	649	1.04e-3	SMART
ZnF_C2H2	655	677	9.88e-5	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000108205

SMART Domains

Protein: ENSMUSP00000103840
Gene: ENSMUSG00000059975

Domain	Start	End	E-Value	Type
KRAB	8	68	5.02e-38	SMART
ZnF_C2H2	179	201	1.84e-4	SMART
ZnF_C2H2	207	229	2.36e-2	SMART
ZnF_C2H2	235	257	1.5e-4	SMART
ZnF_C2H2	263	285	2.79e-4	SMART
ZnF_C2H2	291	313	3.49e-5	SMART
ZnF_C2H2	319	341	6.42e-4	SMART
ZnF_C2H2	347	369	5.59e-4	SMART
ZnF_C2H2	375	397	5.21e-4	SMART
ZnF_C2H2	403	425	3.63e-3	SMART
ZnF_C2H2	431	453	1.82e-3	SMART
ZnF_C2H2	459	481	1.58e-3	SMART
ZnF_C2H2	487	509	2.12e-4	SMART
ZnF_C2H2	515	537	1.82e-3	SMART
ZnF_C2H2	543	565	2.24e-3	SMART
ZnF_C2H2	571	593	8.02e-5	SMART
ZnF_C2H2	599	621	1.04e-3	SMART
ZnF_C2H2	627	649	1.04e-3	SMART
ZnF_C2H2	655	677	9.88e-5	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000108211
AA Change: F133Y

PolyPhen 2 Score 0.108 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000103846
Gene: ENSMUSG00000059975
AA Change: F133Y

Domain	Start	End	E-Value	Type
KRAB	8	68	5.02e-38	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000108212
AA Change: F133Y

PolyPhen 2 Score 0.108 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000103847
Gene: ENSMUSG00000059975
AA Change: F133Y

Domain	Start	End	E-Value	Type
KRAB	8	68	5.02e-38	SMART

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 99.3%
20x: 98.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700029F12Rik	T	C	13: 97,166,738 (GRCm39)	T93A	unknown	Het
Adgrg6	T	A	10: 14,302,515 (GRCm39)	H938L	probably benign	Het
Aff1	A	G	5: 103,997,365 (GRCm39)	Y1153C	probably damaging	Het
Akr1c20	T	A	13: 4,564,388 (GRCm39)	I42L	probably benign	Het
Atad2b	T	A	12: 5,082,064 (GRCm39)	N1324K	probably benign	Het
Aurka	T	A	2: 172,201,760 (GRCm39)	I270F	probably damaging	Het
Camk2g	A	T	14: 20,815,498 (GRCm39)	D189E	probably damaging	Het
Cc2d2b	A	G	19: 40,804,299 (GRCm39)	D942G	unknown	Het
Ccnd1	A	C	7: 144,491,770 (GRCm39)	S90A	probably benign	Het
Cfap77	G	T	2: 28,852,741 (GRCm39)	T177K	probably damaging	Het
Chd9	A	G	8: 91,721,220 (GRCm39)	N1003D	unknown	Het
Chga	C	G	12: 102,525,613 (GRCm39)	Q85E	possibly damaging	Het
Cnot1	T	C	8: 96,496,209 (GRCm39)	S322G	probably benign	Het
Cyp8b1	C	A	9: 121,744,294 (GRCm39)	S346I	probably benign	Het
Cysltr2	T	A	14: 73,266,906 (GRCm39)	H268L	probably damaging	Het
Dnah11	T	C	12: 117,884,704 (GRCm39)	T3783A	probably benign	Het
Dock3	C	T	9: 106,959,713 (GRCm39)	V109I	probably benign	Het
Dscc1	A	G	15: 54,953,674 (GRCm39)	C50R	probably benign	Het
Dtx3l	T	A	16: 35,753,277 (GRCm39)	D443V	probably damaging	Het
Ephb6	A	G	6: 41,592,911 (GRCm39)	H383R	probably benign	Het
Fdft1	A	G	14: 63,400,869 (GRCm39)	I88T	probably damaging	Het
Fmo3	C	A	1: 162,794,524 (GRCm39)	W182L	probably benign	Het
Folh1	T	A	7: 86,406,158 (GRCm39)	Q305L	probably benign	Het
Gale	C	T	4: 135,692,975 (GRCm39)	Q73*	probably null	Het
Gm9195	T	C	14: 72,699,885 (GRCm39)	M1154V	unknown	Het
Grip1	G	A	10: 119,874,569 (GRCm39)	E778K	possibly damaging	Het
Gtsf1l	T	C	2: 162,929,052 (GRCm39)	T144A	probably benign	Het
Itpkc	T	C	7: 26,927,222 (GRCm39)	T231A	probably benign	Het
Kng2	C	A	16: 22,822,737 (GRCm39)	C205F	probably damaging	Het
Ldlrap1	T	G	4: 134,476,661 (GRCm39)	N244T	probably benign	Het
Lingo2	A	G	4: 35,709,750 (GRCm39)	F77L	possibly damaging	Het
Lpcat2b	A	T	5: 107,581,438 (GRCm39)	T256S	possibly damaging	Het
Lrit2	A	G	14: 36,790,936 (GRCm39)	D205G	probably damaging	Het
Lrrc34	T	C	3: 30,697,467 (GRCm39)	D88G	probably damaging	Het
Macf1	T	C	4: 123,366,136 (GRCm39)	E2875G	probably benign	Het
Med13	G	T	11: 86,177,345 (GRCm39)	Q1584K	possibly damaging	Het
Naip5	C	G	13: 100,367,269 (GRCm39)	A276P	probably damaging	Het
Ndufv2	A	G	17: 66,394,503 (GRCm39)	Y121H	probably damaging	Het
Or4f52	T	A	2: 111,061,521 (GRCm39)	I206F	probably damaging	Het
Peg12	T	C	7: 62,113,297 (GRCm39)	T267A	unknown	Het
Pkig	A	G	2: 163,567,375 (GRCm39)		probably null	Het
Prkch	C	G	12: 73,747,078 (GRCm39)	T319S	probably benign	Het
Rhobtb1	T	C	10: 69,115,202 (GRCm39)	S534P	probably damaging	Het
Riok2	A	G	17: 17,607,814 (GRCm39)	N454S	probably benign	Het
Ryr2	T	A	13: 11,701,935 (GRCm39)	K2907N	possibly damaging	Het
Sdc3	C	T	4: 130,546,048 (GRCm39)	P136S	unknown	Het
Slc37a3	G	A	6: 39,322,844 (GRCm39)	L371F	probably damaging	Het
Slc3a1	A	T	17: 85,371,419 (GRCm39)	H657L	probably damaging	Het
Slc7a13	T	A	4: 19,819,033 (GRCm39)	Y78N	probably benign	Het
Slfn8	G	T	11: 82,907,838 (GRCm39)	A235E	probably damaging	Het
Snap91	T	A	9: 86,707,094 (GRCm39)	D232V	possibly damaging	Het
Stap2	C	A	17: 56,309,914 (GRCm39)	S79I	probably damaging	Het
Stra6l	T	C	4: 45,884,602 (GRCm39)	V544A	probably damaging	Het
Syn2	T	C	6: 115,251,219 (GRCm39)		probably null	Het
Tctn1	G	A	5: 122,388,527 (GRCm39)	P284S	possibly damaging	Het
Terb2	G	A	2: 122,018,475 (GRCm39)	M77I	probably benign	Het
Trim80	G	T	11: 115,338,757 (GRCm39)	R529L	possibly damaging	Het
Washc2	T	C	6: 116,186,048 (GRCm39)	L86P	possibly damaging	Het
Zfp987	T	A	4: 146,060,752 (GRCm39)	N61K	probably benign	Het

Other mutations in Zfp74

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0355:Zfp74	UTSW	7	29,653,466 (GRCm39)	start gained	probably benign
R0387:Zfp74	UTSW	7	29,634,179 (GRCm39)	missense	probably benign	0.05
R0948:Zfp74	UTSW	7	29,635,362 (GRCm39)	critical splice donor site	probably null
R1757:Zfp74	UTSW	7	29,634,486 (GRCm39)	missense	probably benign	0.01
R1813:Zfp74	UTSW	7	29,634,569 (GRCm39)	missense	probably damaging	1.00
R1893:Zfp74	UTSW	7	29,635,470 (GRCm39)	critical splice acceptor site	probably null
R1896:Zfp74	UTSW	7	29,634,569 (GRCm39)	missense	probably damaging	1.00
R1958:Zfp74	UTSW	7	29,635,136 (GRCm39)	missense	probably benign	0.08
R2092:Zfp74	UTSW	7	29,653,349 (GRCm39)	start gained	probably benign
R2111:Zfp74	UTSW	7	29,634,443 (GRCm39)	nonsense	probably null
R4894:Zfp74	UTSW	7	29,635,470 (GRCm39)	critical splice acceptor site	probably benign
R5121:Zfp74	UTSW	7	29,631,932 (GRCm39)	splice site	probably null
R5123:Zfp74	UTSW	7	29,634,158 (GRCm39)	missense	probably damaging	1.00
R5129:Zfp74	UTSW	7	29,631,880 (GRCm39)	missense	probably benign	0.00
R5213:Zfp74	UTSW	7	29,634,668 (GRCm39)	missense	probably damaging	1.00
R5460:Zfp74	UTSW	7	29,635,316 (GRCm39)	missense	probably benign	0.04
R5519:Zfp74	UTSW	7	29,634,559 (GRCm39)	missense	probably damaging	0.99
R5589:Zfp74	UTSW	7	29,633,990 (GRCm39)	missense	probably damaging	1.00
R6287:Zfp74	UTSW	7	29,635,201 (GRCm39)	missense	probably benign
R6330:Zfp74	UTSW	7	29,637,412 (GRCm39)	missense	probably damaging	1.00
R6370:Zfp74	UTSW	7	29,631,835 (GRCm39)	missense	probably damaging	0.96
R6407:Zfp74	UTSW	7	29,635,048 (GRCm39)	missense	probably damaging	1.00
R6694:Zfp74	UTSW	7	29,634,559 (GRCm39)	missense	probably damaging	0.99
R6791:Zfp74	UTSW	7	29,633,860 (GRCm39)	missense	probably benign	0.02
R7144:Zfp74	UTSW	7	29,634,590 (GRCm39)	missense	probably damaging	0.98
R7662:Zfp74	UTSW	7	29,653,278 (GRCm39)	critical splice donor site	probably null
R7667:Zfp74	UTSW	7	29,634,608 (GRCm39)	missense	probably damaging	1.00
R7898:Zfp74	UTSW	7	29,635,380 (GRCm39)	nonsense	probably null
R7940:Zfp74	UTSW	7	29,631,867 (GRCm39)	missense	probably benign	0.07
R8676:Zfp74	UTSW	7	29,634,079 (GRCm39)	missense	probably damaging	1.00
R8864:Zfp74	UTSW	7	29,634,235 (GRCm39)	missense	probably damaging	1.00
R8940:Zfp74	UTSW	7	29,634,772 (GRCm39)	missense	possibly damaging	0.96
R9748:Zfp74	UTSW	7	29,634,751 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGAGACAGACAGCTTTCTGGTAC -3'
(R):5'- TCTGACGCTTTAAAGGTCCTG -3'

Sequencing Primer
(F):5'- GACAGACAGCTTTCTGGTACTATGAC -3'
(R):5'- GACGCTTTAAAGGTCCTGTATTTAG -3'

Posted On

2022-11-14