Mutagenetix > Incidental Mutation

Incidental Mutation 'R9766:Wdfy3'

733265

Institutional Source

Beutler Lab

Gene Symbol

Wdfy3

Ensembl Gene

ENSMUSG00000043940

Gene Name

WD repeat and FYVE domain containing 3

Synonyms

2610509D04Rik, Ggtb3, Bchs, D5Ertd66e, Bwf1, Alfy

MMRRC Submission

Accession Numbers

Essential gene?

Probably essential (E-score: 0.942) question?

Stock #

R9766 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

101980822-102217787 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 102042866 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Isoleucine at position 1962 (V1962I)

Ref Sequence

ENSEMBL: ENSMUSP00000052607 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000053177] [ENSMUST00000174598] [ENSMUST00000212024]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000053177
AA Change: V1962I

PolyPhen 2 Score 0.902 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000052607
Gene: ENSMUSG00000043940
AA Change: V1962I

Domain	Start	End	E-Value	Type
low complexity region	463	481	N/A	INTRINSIC
low complexity region	1408	1417	N/A	INTRINSIC
low complexity region	1629	1644	N/A	INTRINSIC
Pfam:PH_BEACH	2517	2638	3.1e-17	PFAM
Beach	2677	2958	2.54e-217	SMART
WD40	3054	3088	1.28e1	SMART
WD40	3098	3137	7.73e-6	SMART
WD40	3140	3178	8.29e-1	SMART
WD40	3183	3227	3.09e-1	SMART
low complexity region	3253	3274	N/A	INTRINSIC
low complexity region	3307	3318	N/A	INTRINSIC
WD40	3381	3420	1.33e1	SMART
FYVE	3428	3497	3.18e-27	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000174598
AA Change: V1962I

PolyPhen 2 Score 0.854 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000134244
Gene: ENSMUSG00000043940
AA Change: V1962I

Domain	Start	End	E-Value	Type
low complexity region	463	481	N/A	INTRINSIC
Pfam:DUF4704	1392	1597	6.6e-11	PFAM
low complexity region	1629	1644	N/A	INTRINSIC
Pfam:PH_BEACH	2588	2656	1.8e-14	PFAM
Beach	2695	2976	2.54e-217	SMART
WD40	3072	3106	1.28e1	SMART
WD40	3116	3155	7.73e-6	SMART
WD40	3158	3196	8.29e-1	SMART
WD40	3201	3245	3.09e-1	SMART
low complexity region	3271	3292	N/A	INTRINSIC
low complexity region	3325	3336	N/A	INTRINSIC
WD40	3399	3438	1.33e1	SMART
FYVE	3446	3515	3.18e-27	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000212024
AA Change: V1947I

PolyPhen 2 Score 0.758 (Sensitivity: 0.85; Specificity: 0.92)

Coding Region Coverage

1x: 99.9%
3x: 99.8%
10x: 99.3%
20x: 98.5%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a phosphatidylinositol 3-phosphate-binding protein that functions as a master conductor for aggregate clearance by autophagy. This protein shuttles from the nuclear membrane to colocalize with aggregated proteins, where it complexes with other autophagic components to achieve macroautophagy-mediated clearance of these aggregated proteins. However, it is not necessary for starvation-induced macroautophagy. [provided by RefSeq, May 2010]
PHENOTYPE: Mice homozygous for hypomorphic mutations of this gene exhibit perinatal lethality, altered neural progenitor divisions and neuronal migration, a regionally enlarged cerebral cortex, and focal cortical dysplasias. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3425401B19Rik	A	G	14: 32,385,788 (GRCm39)	V59A	probably benign	Het
Aatk	A	T	11: 119,902,565 (GRCm39)	H610Q	probably benign	Het
Acox1	G	A	11: 116,071,867 (GRCm39)	A187V	probably damaging	Het
Acsbg3	A	G	17: 57,189,177 (GRCm39)	Y195C	probably benign	Het
AI661453	C	T	17: 47,757,570 (GRCm39)	R76W	probably damaging	Het
Anapc1	C	T	2: 128,500,221 (GRCm39)	R808Q	probably damaging	Het
Ankrd26	T	C	6: 118,500,067 (GRCm39)	T1135A	possibly damaging	Het
Arhgap17	A	G	7: 122,921,148 (GRCm39)	V113A	probably benign	Het
Art3	T	C	5: 92,562,138 (GRCm39)	C379R	unknown	Het
Bltp3a	T	C	17: 28,105,799 (GRCm39)	L775P	probably damaging	Het
Bmp5	A	G	9: 75,800,982 (GRCm39)	I371V	probably damaging	Het
Ccdc88b	T	A	19: 6,833,096 (GRCm39)	E281V	probably damaging	Het
Ces1e	A	T	8: 93,946,031 (GRCm39)	Y171N	probably damaging	Het
Cib3	A	T	8: 72,961,034 (GRCm39)	F68I	possibly damaging	Het
Crtac1	A	T	19: 42,402,557 (GRCm39)	L17Q	possibly damaging	Het
Crybg3	A	T	16: 59,376,207 (GRCm39)	S1682R	probably benign	Het
Ddx60	G	A	8: 62,465,312 (GRCm39)	G1323D	probably damaging	Het
Dock3	A	C	9: 106,788,483 (GRCm39)	D161E	probably benign	Het
Dpep3	A	C	8: 106,705,369 (GRCm39)	L127R	probably damaging	Het
Dus2	A	G	8: 106,772,568 (GRCm39)	D226G	probably damaging	Het
Ebna1bp2	T	C	4: 118,480,821 (GRCm39)	V184A	possibly damaging	Het
Edem1	T	A	6: 108,823,648 (GRCm39)	I348N	probably damaging	Het
Efcab8	A	T	2: 153,656,362 (GRCm39)	M599L	unknown	Het
Eif2ak4	A	T	2: 118,261,313 (GRCm39)	K618*	probably null	Het
Fbxw27	T	C	9: 109,602,215 (GRCm39)	K253E	possibly damaging	Het
Fgf5	A	G	5: 98,423,113 (GRCm39)	E166G	possibly damaging	Het
Flot1	G	T	17: 36,141,555 (GRCm39)	E321*	probably null	Het
Gm14443	T	C	2: 175,012,248 (GRCm39)	E66G	probably benign	Het
Gprc6a	G	A	10: 51,491,884 (GRCm39)	P622S	probably damaging	Het
Gsta2	A	T	9: 78,244,876 (GRCm39)	Y79*	probably null	Het
Hecw2	A	G	1: 53,904,287 (GRCm39)	S1154P	probably damaging	Het
Hrob	A	T	11: 102,146,586 (GRCm39)	Q287H	possibly damaging	Het
Kcnd2	T	C	6: 21,216,367 (GRCm39)	S24P	probably benign	Het
Knl1	A	G	2: 118,900,381 (GRCm39)	D694G	probably damaging	Het
L1td1	T	C	4: 98,624,753 (GRCm39)	I316T	probably benign	Het
Lrsam1	G	T	2: 32,818,077 (GRCm39)	Q660K	probably benign	Het
Muc16	T	A	9: 18,548,153 (GRCm39)	T6047S	probably benign	Het
Muc16	T	A	9: 18,552,383 (GRCm39)	T4637S	probably benign	Het
Myo5a	T	C	9: 75,078,914 (GRCm39)	Y891H	probably damaging	Het
Nkd2	G	T	13: 73,995,131 (GRCm39)	D22E	possibly damaging	Het
Patl2	A	T	2: 121,954,212 (GRCm39)	V453E	probably damaging	Het
Pcnx2	A	G	8: 126,488,313 (GRCm39)	Y1744H	probably damaging	Het
Phf8-ps	T	G	17: 33,285,647 (GRCm39)	N385T	probably damaging	Het
Pira2	T	A	7: 3,845,517 (GRCm39)	H289L	possibly damaging	Het
Pitpnm1	T	C	19: 4,158,117 (GRCm39)	I569T	probably benign	Het
Ppp4r4	T	C	12: 103,562,735 (GRCm39)	V568A	probably benign	Het
Ptf1a	G	A	2: 19,451,062 (GRCm39)	G131S	probably benign	Het
Rasgrf2	G	T	13: 92,160,188 (GRCm39)	H396N	probably damaging	Het
Rfx1	C	T	8: 84,814,376 (GRCm39)	T315M	probably damaging	Het
Rnf207	C	A	4: 152,400,402 (GRCm39)	R148L	probably damaging	Het
Scn11a	T	G	9: 119,584,181 (GRCm39)	N1478T	probably damaging	Het
Sdr39u1	C	T	14: 56,135,194 (GRCm39)	V250I	probably benign	Het
Serinc3	T	C	2: 163,471,095 (GRCm39)	T276A	probably damaging	Het
Speer4a3	AACT	A	5: 26,155,849 (GRCm39)		probably benign	Het
Stambp	C	A	6: 83,534,469 (GRCm39)	A273S	probably benign	Het
Taf2	T	C	15: 54,910,881 (GRCm39)		probably null	Het
Tex26	A	T	5: 149,386,642 (GRCm39)	Y232F	probably damaging	Het
Tmem258	T	C	19: 10,184,578 (GRCm39)	L55P	probably damaging	Het
Trappc8	A	G	18: 20,979,630 (GRCm39)	I755T	possibly damaging	Het
Ubr4	T	C	4: 139,194,595 (GRCm39)	Y1163H	unknown	Het
Usp14	G	A	18: 10,005,630 (GRCm39)	T254I	probably benign	Het
Vcan	A	G	13: 89,839,247 (GRCm39)	L2099P	probably benign	Het
Zfp407	T	C	18: 84,577,574 (GRCm39)	T1180A	probably benign	Het
Zfp644	C	A	5: 106,784,691 (GRCm39)	G619C	probably damaging	Het

Other mutations in Wdfy3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00332:Wdfy3	APN	5	102,063,204 (GRCm39)	critical splice donor site	probably null
IGL00567:Wdfy3	APN	5	102,059,896 (GRCm39)	splice site	probably benign
IGL01288:Wdfy3	APN	5	102,049,857 (GRCm39)	splice site	probably null
IGL01323:Wdfy3	APN	5	102,042,930 (GRCm39)	missense	probably damaging	1.00
IGL01352:Wdfy3	APN	5	102,091,986 (GRCm39)	missense	probably damaging	1.00
IGL01553:Wdfy3	APN	5	102,047,897 (GRCm39)	missense	probably benign
IGL01560:Wdfy3	APN	5	102,105,352 (GRCm39)	nonsense	probably null
IGL01566:Wdfy3	APN	5	102,044,454 (GRCm39)	splice site	probably benign
IGL01616:Wdfy3	APN	5	102,061,126 (GRCm39)	missense	probably damaging	0.97
IGL01630:Wdfy3	APN	5	102,055,354 (GRCm39)	missense	probably benign
IGL01791:Wdfy3	APN	5	102,085,278 (GRCm39)	missense	probably damaging	1.00
IGL01820:Wdfy3	APN	5	102,071,947 (GRCm39)	missense	probably benign	0.11
IGL01953:Wdfy3	APN	5	102,042,894 (GRCm39)	nonsense	probably null
IGL02121:Wdfy3	APN	5	102,046,376 (GRCm39)	missense	possibly damaging	0.85
IGL02167:Wdfy3	APN	5	102,109,023 (GRCm39)	missense	probably damaging	0.98
IGL02321:Wdfy3	APN	5	102,070,475 (GRCm39)	missense	probably damaging	0.99
IGL02327:Wdfy3	APN	5	102,036,058 (GRCm39)	missense	probably damaging	1.00
IGL02651:Wdfy3	APN	5	102,044,341 (GRCm39)	missense	probably benign	0.37
IGL02801:Wdfy3	APN	5	102,055,453 (GRCm39)	missense	probably damaging	1.00
IGL02839:Wdfy3	APN	5	102,116,786 (GRCm39)	missense	probably damaging	1.00
IGL02870:Wdfy3	APN	5	102,003,337 (GRCm39)	missense	probably damaging	1.00
IGL02997:Wdfy3	APN	5	102,042,778 (GRCm39)	missense	probably null	1.00
IGL03064:Wdfy3	APN	5	102,083,863 (GRCm39)	missense	probably damaging	0.99
IGL03090:Wdfy3	APN	5	102,014,142 (GRCm39)	missense	probably damaging	1.00
IGL03211:Wdfy3	APN	5	101,992,778 (GRCm39)	splice site	probably benign
IGL03237:Wdfy3	APN	5	101,992,465 (GRCm39)	missense	probably damaging	1.00
IGL03264:Wdfy3	APN	5	102,048,016 (GRCm39)	missense	probably damaging	1.00
Esurient	UTSW	5	102,091,969 (GRCm39)	missense	probably damaging	1.00
IGL02988:Wdfy3	UTSW	5	102,077,847 (GRCm39)	missense	probably damaging	0.99
PIT4382001:Wdfy3	UTSW	5	102,030,827 (GRCm39)	frame shift	probably null
R0010:Wdfy3	UTSW	5	101,996,215 (GRCm39)	missense	probably damaging	1.00
R0010:Wdfy3	UTSW	5	101,996,215 (GRCm39)	missense	probably damaging	1.00
R0025:Wdfy3	UTSW	5	101,992,912 (GRCm39)	missense	probably damaging	0.98
R0031:Wdfy3	UTSW	5	102,037,161 (GRCm39)	missense	probably damaging	0.97
R0047:Wdfy3	UTSW	5	102,091,899 (GRCm39)	missense	probably damaging	1.00
R0047:Wdfy3	UTSW	5	102,091,899 (GRCm39)	missense	probably damaging	1.00
R0053:Wdfy3	UTSW	5	101,992,480 (GRCm39)	missense	probably damaging	0.97
R0078:Wdfy3	UTSW	5	102,035,971 (GRCm39)	missense	possibly damaging	0.57
R0147:Wdfy3	UTSW	5	102,065,277 (GRCm39)	missense	probably benign	0.05
R0148:Wdfy3	UTSW	5	102,065,277 (GRCm39)	missense	probably benign	0.05
R0279:Wdfy3	UTSW	5	102,015,958 (GRCm39)	missense	probably damaging	1.00
R0380:Wdfy3	UTSW	5	102,096,832 (GRCm39)	missense	probably damaging	0.99
R0472:Wdfy3	UTSW	5	102,105,309 (GRCm39)	missense	probably benign	0.13
R0513:Wdfy3	UTSW	5	102,038,655 (GRCm39)	missense	probably damaging	0.96
R0594:Wdfy3	UTSW	5	102,054,051 (GRCm39)	missense	possibly damaging	0.94
R0601:Wdfy3	UTSW	5	101,984,038 (GRCm39)	missense	probably benign
R0787:Wdfy3	UTSW	5	102,105,254 (GRCm39)	missense	probably damaging	1.00
R0825:Wdfy3	UTSW	5	102,017,917 (GRCm39)	missense	probably damaging	1.00
R1122:Wdfy3	UTSW	5	102,030,832 (GRCm39)	missense	possibly damaging	0.94
R1167:Wdfy3	UTSW	5	102,023,797 (GRCm39)	missense	probably benign
R1350:Wdfy3	UTSW	5	102,046,418 (GRCm39)	missense	probably damaging	1.00
R1422:Wdfy3	UTSW	5	102,032,080 (GRCm39)	splice site	probably benign
R1446:Wdfy3	UTSW	5	101,999,176 (GRCm39)	missense	possibly damaging	0.68
R1452:Wdfy3	UTSW	5	102,085,604 (GRCm39)	missense	possibly damaging	0.91
R1457:Wdfy3	UTSW	5	102,065,445 (GRCm39)	missense	possibly damaging	0.57
R1543:Wdfy3	UTSW	5	101,991,947 (GRCm39)	missense	probably benign
R1633:Wdfy3	UTSW	5	102,129,414 (GRCm39)	missense	probably damaging	1.00
R1643:Wdfy3	UTSW	5	102,023,781 (GRCm39)	missense	possibly damaging	0.62
R1656:Wdfy3	UTSW	5	102,089,313 (GRCm39)	missense	probably damaging	1.00
R1720:Wdfy3	UTSW	5	102,074,391 (GRCm39)	frame shift	probably null
R1743:Wdfy3	UTSW	5	101,991,931 (GRCm39)	missense	probably benign	0.12
R1745:Wdfy3	UTSW	5	102,096,795 (GRCm39)	missense	probably damaging	0.96
R1850:Wdfy3	UTSW	5	102,042,865 (GRCm39)	missense	probably damaging	1.00
R1852:Wdfy3	UTSW	5	102,063,242 (GRCm39)	missense	probably benign	0.00
R1854:Wdfy3	UTSW	5	102,036,052 (GRCm39)	missense	probably benign	0.05
R1880:Wdfy3	UTSW	5	102,065,301 (GRCm39)	missense	probably benign	0.05
R1930:Wdfy3	UTSW	5	102,089,358 (GRCm39)	missense	probably damaging	1.00
R1931:Wdfy3	UTSW	5	102,089,358 (GRCm39)	missense	probably damaging	1.00
R1956:Wdfy3	UTSW	5	102,067,275 (GRCm39)	missense	probably benign	0.30
R1965:Wdfy3	UTSW	5	102,099,178 (GRCm39)	missense	probably damaging	1.00
R1997:Wdfy3	UTSW	5	102,116,812 (GRCm39)	missense	probably damaging	1.00
R2015:Wdfy3	UTSW	5	102,008,352 (GRCm39)	missense	probably null	1.00
R2087:Wdfy3	UTSW	5	102,042,926 (GRCm39)	missense	probably damaging	1.00
R2156:Wdfy3	UTSW	5	102,046,291 (GRCm39)	critical splice donor site	probably null
R2192:Wdfy3	UTSW	5	102,055,408 (GRCm39)	missense	possibly damaging	0.55
R2313:Wdfy3	UTSW	5	102,037,150 (GRCm39)	missense	probably damaging	1.00
R2332:Wdfy3	UTSW	5	102,036,189 (GRCm39)	splice site	probably benign
R2406:Wdfy3	UTSW	5	102,036,125 (GRCm39)	missense	probably damaging	1.00
R2679:Wdfy3	UTSW	5	102,017,902 (GRCm39)	missense	probably damaging	1.00
R2857:Wdfy3	UTSW	5	102,023,796 (GRCm39)	missense	probably benign	0.04
R2937:Wdfy3	UTSW	5	102,091,988 (GRCm39)	missense	probably benign	0.07
R3765:Wdfy3	UTSW	5	102,009,266 (GRCm39)	missense	probably damaging	1.00
R3795:Wdfy3	UTSW	5	102,085,466 (GRCm39)	missense	probably damaging	1.00
R3937:Wdfy3	UTSW	5	102,092,105 (GRCm39)	nonsense	probably null
R3947:Wdfy3	UTSW	5	102,017,902 (GRCm39)	missense	probably damaging	1.00
R4024:Wdfy3	UTSW	5	102,071,961 (GRCm39)	splice site	probably benign
R4065:Wdfy3	UTSW	5	102,070,313 (GRCm39)	missense	probably benign	0.08
R4066:Wdfy3	UTSW	5	102,070,313 (GRCm39)	missense	probably benign	0.08
R4110:Wdfy3	UTSW	5	102,047,924 (GRCm39)	critical splice donor site	probably null
R4235:Wdfy3	UTSW	5	102,070,500 (GRCm39)	critical splice acceptor site	probably null
R4420:Wdfy3	UTSW	5	102,058,850 (GRCm39)	missense	probably damaging	0.97
R4620:Wdfy3	UTSW	5	102,054,011 (GRCm39)	missense	probably damaging	0.99
R4624:Wdfy3	UTSW	5	102,031,949 (GRCm39)	missense	possibly damaging	0.52
R4626:Wdfy3	UTSW	5	102,091,800 (GRCm39)	missense	probably damaging	1.00
R4727:Wdfy3	UTSW	5	102,077,894 (GRCm39)	missense	probably damaging	0.99
R4794:Wdfy3	UTSW	5	102,091,809 (GRCm39)	missense	probably damaging	1.00
R4869:Wdfy3	UTSW	5	102,042,787 (GRCm39)	missense	probably damaging	0.98
R4971:Wdfy3	UTSW	5	102,096,838 (GRCm39)	nonsense	probably null
R4973:Wdfy3	UTSW	5	102,090,985 (GRCm39)	missense	probably benign	0.00
R4976:Wdfy3	UTSW	5	102,090,985 (GRCm39)	missense	probably benign	0.00
R4984:Wdfy3	UTSW	5	102,090,985 (GRCm39)	missense	probably benign	0.00
R4986:Wdfy3	UTSW	5	102,090,985 (GRCm39)	missense	probably benign	0.00
R5068:Wdfy3	UTSW	5	102,042,803 (GRCm39)	missense	probably benign	0.15
R5105:Wdfy3	UTSW	5	102,003,415 (GRCm39)	missense	probably damaging	1.00
R5120:Wdfy3	UTSW	5	102,015,972 (GRCm39)	missense	possibly damaging	0.85
R5134:Wdfy3	UTSW	5	102,091,969 (GRCm39)	missense	probably damaging	1.00
R5139:Wdfy3	UTSW	5	101,997,133 (GRCm39)	critical splice donor site	probably null
R5235:Wdfy3	UTSW	5	101,994,972 (GRCm39)	missense	probably null	0.03
R5303:Wdfy3	UTSW	5	102,100,849 (GRCm39)	missense	probably damaging	1.00
R5368:Wdfy3	UTSW	5	102,020,724 (GRCm39)	missense	probably damaging	1.00
R5426:Wdfy3	UTSW	5	102,067,312 (GRCm39)	missense	probably damaging	0.97
R5442:Wdfy3	UTSW	5	102,044,425 (GRCm39)	missense	probably benign	0.04
R5487:Wdfy3	UTSW	5	101,984,140 (GRCm39)	missense	probably damaging	1.00
R5509:Wdfy3	UTSW	5	102,009,314 (GRCm39)	missense	possibly damaging	0.69
R5877:Wdfy3	UTSW	5	102,017,855 (GRCm39)	missense	probably damaging	1.00
R5988:Wdfy3	UTSW	5	102,032,004 (GRCm39)	missense	probably benign	0.00
R6017:Wdfy3	UTSW	5	101,999,225 (GRCm39)	missense	probably benign	0.01
R6019:Wdfy3	UTSW	5	101,997,289 (GRCm39)	missense	probably damaging	1.00
R6199:Wdfy3	UTSW	5	102,020,831 (GRCm39)	missense	possibly damaging	0.93
R6228:Wdfy3	UTSW	5	102,046,295 (GRCm39)	missense	possibly damaging	0.67
R6258:Wdfy3	UTSW	5	102,020,831 (GRCm39)	missense	possibly damaging	0.93
R6259:Wdfy3	UTSW	5	102,020,831 (GRCm39)	missense	possibly damaging	0.93
R6298:Wdfy3	UTSW	5	102,116,812 (GRCm39)	missense	probably damaging	1.00
R6479:Wdfy3	UTSW	5	102,061,045 (GRCm39)	missense	probably damaging	1.00
R6550:Wdfy3	UTSW	5	102,101,032 (GRCm39)	missense	probably benign	0.19
R6776:Wdfy3	UTSW	5	102,031,911 (GRCm39)	missense	possibly damaging	0.57
R6793:Wdfy3	UTSW	5	102,065,297 (GRCm39)	nonsense	probably null
R6809:Wdfy3	UTSW	5	102,071,813 (GRCm39)	missense	possibly damaging	0.63
R6836:Wdfy3	UTSW	5	102,100,865 (GRCm39)	missense	probably damaging	1.00
R6897:Wdfy3	UTSW	5	101,991,932 (GRCm39)	missense	probably benign	0.10
R7014:Wdfy3	UTSW	5	102,042,775 (GRCm39)	critical splice donor site	probably null
R7034:Wdfy3	UTSW	5	102,055,384 (GRCm39)	missense	probably damaging	1.00
R7035:Wdfy3	UTSW	5	102,003,415 (GRCm39)	missense	probably damaging	1.00
R7135:Wdfy3	UTSW	5	102,063,303 (GRCm39)	missense	probably damaging	1.00
R7182:Wdfy3	UTSW	5	102,091,758 (GRCm39)	missense	possibly damaging	0.51
R7217:Wdfy3	UTSW	5	102,049,785 (GRCm39)	missense	probably damaging	1.00
R7236:Wdfy3	UTSW	5	101,984,074 (GRCm39)	missense	probably damaging	0.99
R7264:Wdfy3	UTSW	5	102,003,389 (GRCm39)	missense	probably benign	0.02
R7418:Wdfy3	UTSW	5	102,105,366 (GRCm39)	missense	probably benign	0.08
R7533:Wdfy3	UTSW	5	102,030,354 (GRCm39)	missense	probably benign	0.27
R7543:Wdfy3	UTSW	5	102,083,925 (GRCm39)	missense	probably benign	0.00
R7625:Wdfy3	UTSW	5	102,003,252 (GRCm39)	splice site	probably null
R7788:Wdfy3	UTSW	5	101,996,223 (GRCm39)	missense	probably damaging	0.99
R7810:Wdfy3	UTSW	5	102,099,265 (GRCm39)	nonsense	probably null
R7810:Wdfy3	UTSW	5	102,042,940 (GRCm39)	missense	probably benign	0.01
R8204:Wdfy3	UTSW	5	102,000,451 (GRCm39)	missense	probably benign	0.00
R8268:Wdfy3	UTSW	5	102,089,476 (GRCm39)	missense	probably damaging	1.00
R8286:Wdfy3	UTSW	5	102,085,287 (GRCm39)	missense	probably benign
R8507:Wdfy3	UTSW	5	102,020,767 (GRCm39)	missense	probably benign	0.05
R8514:Wdfy3	UTSW	5	101,999,219 (GRCm39)	missense	possibly damaging	0.92
R8536:Wdfy3	UTSW	5	102,033,064 (GRCm39)	missense	probably benign
R8710:Wdfy3	UTSW	5	102,030,349 (GRCm39)	missense	probably damaging	1.00
R8735:Wdfy3	UTSW	5	102,077,951 (GRCm39)	missense	probably benign	0.00
R8749:Wdfy3	UTSW	5	102,030,446 (GRCm39)	missense	probably damaging	1.00
R8931:Wdfy3	UTSW	5	102,065,421 (GRCm39)	missense	probably benign	0.11
R8943:Wdfy3	UTSW	5	101,993,231 (GRCm39)	intron	probably benign
R8968:Wdfy3	UTSW	5	102,011,983 (GRCm39)	missense	probably benign	0.05
R8979:Wdfy3	UTSW	5	102,096,764 (GRCm39)	missense	probably damaging	1.00
R8998:Wdfy3	UTSW	5	101,993,058 (GRCm39)	missense	probably benign	0.05
R9045:Wdfy3	UTSW	5	101,995,040 (GRCm39)	missense	probably damaging	1.00
R9068:Wdfy3	UTSW	5	102,000,451 (GRCm39)	missense	probably benign	0.34
R9105:Wdfy3	UTSW	5	102,030,512 (GRCm39)	missense	probably benign	0.05
R9122:Wdfy3	UTSW	5	102,091,831 (GRCm39)	missense	probably damaging	1.00
R9209:Wdfy3	UTSW	5	102,078,830 (GRCm39)	missense	probably benign	0.01
R9249:Wdfy3	UTSW	5	101,996,359 (GRCm39)	missense	possibly damaging	0.82
R9348:Wdfy3	UTSW	5	102,089,358 (GRCm39)	missense	probably damaging	1.00
R9481:Wdfy3	UTSW	5	102,000,478 (GRCm39)	missense	probably benign	0.19
R9490:Wdfy3	UTSW	5	102,078,716 (GRCm39)	missense	probably benign	0.29
R9524:Wdfy3	UTSW	5	102,055,333 (GRCm39)	missense	probably benign	0.03
R9545:Wdfy3	UTSW	5	102,100,957 (GRCm39)	missense
R9548:Wdfy3	UTSW	5	102,033,059 (GRCm39)	missense	probably damaging	0.99
R9636:Wdfy3	UTSW	5	102,047,899 (GRCm39)	missense	probably benign
R9750:Wdfy3	UTSW	5	102,077,960 (GRCm39)	missense	probably benign	0.00
R9771:Wdfy3	UTSW	5	102,000,195 (GRCm39)	missense	probably damaging	1.00
Z1177:Wdfy3	UTSW	5	102,048,107 (GRCm39)	missense	probably benign	0.39

Predicted Primers

PCR Primer
(F):5'- TCTGCCCATGTCATCCAGAAG -3'
(R):5'- AGGTGCTACATAAACTCTGATTCG -3'

Sequencing Primer
(F):5'- CTGGAGGCCTAAAAGTATTCTGCAC -3'
(R):5'- GCTACATAAACTCTGATTCGATCCCG -3'

Posted On

2022-11-14