Incidental Mutation 'R9772:Mettl25'
| ID |
733488 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Mettl25
|
| Ensembl Gene |
ENSMUSG00000036009 |
| Gene Name |
methyltransferase like 25 |
| Synonyms |
BC067068 |
| MMRRC Submission |
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.096)
|
| Stock # |
R9772 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Not validated
|
| Chromosome |
10 |
| Chromosomal Location |
105599050-105677241 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to C
at 105633127 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Arginine to Glycine
at position 439
(R439G)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000038665
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000046638]
[ENSMUST00000176924]
|
| AlphaFold |
no structure available at present |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000046638
AA Change: R439G
PolyPhen 2
Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
|
| SMART Domains |
Protein: ENSMUSP00000038665
Gene: ENSMUSG00000036009
AA Change: R439G
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Methyltransf_32
|
149 |
413 |
1.9e-30 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000176924
|
| Coding Region Coverage |
- 1x: 99.9%
- 3x: 99.8%
- 10x: 99.4%
- 20x: 98.5%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 39 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Adamtsl2 |
A |
T |
2: 26,985,666 (GRCm39) |
I517F |
probably benign |
Het |
| Ankdd1a |
A |
G |
9: 65,408,749 (GRCm39) |
C506R |
probably damaging |
Het |
| Arid3c |
T |
C |
4: 41,724,723 (GRCm39) |
N371D |
probably damaging |
Het |
| Ccdc117 |
C |
T |
11: 5,492,042 (GRCm39) |
R6Q |
possibly damaging |
Het |
| Ccdc78 |
C |
T |
17: 26,005,665 (GRCm39) |
R26* |
probably null |
Het |
| Clca4a |
C |
A |
3: 144,676,422 (GRCm39) |
W86L |
probably damaging |
Het |
| Clrn2 |
C |
T |
5: 45,611,369 (GRCm39) |
Q73* |
probably null |
Het |
| Cpne6 |
A |
G |
14: 55,754,117 (GRCm39) |
D478G |
probably benign |
Het |
| Cyp2c40 |
T |
A |
19: 39,792,348 (GRCm39) |
I199L |
probably benign |
Het |
| Dna2 |
T |
C |
10: 62,786,522 (GRCm39) |
V90A |
probably benign |
Het |
| Dydc1 |
A |
G |
14: 40,804,248 (GRCm39) |
E90G |
probably damaging |
Het |
| Egf |
A |
T |
3: 129,499,756 (GRCm39) |
S795T |
probably benign |
Het |
| Gm13090 |
G |
T |
4: 151,175,565 (GRCm39) |
A102S |
unknown |
Het |
| Hectd4 |
A |
T |
5: 121,448,744 (GRCm39) |
Y364F |
probably benign |
Het |
| Iqgap3 |
C |
A |
3: 88,016,176 (GRCm39) |
F986L |
probably damaging |
Het |
| Krt79 |
T |
G |
15: 101,839,196 (GRCm39) |
E424D |
probably benign |
Het |
| Ltf |
T |
C |
9: 110,869,425 (GRCm39) |
S87P |
unknown |
Het |
| Mesp2 |
A |
G |
7: 79,461,348 (GRCm39) |
I224M |
possibly damaging |
Het |
| Mettl27 |
C |
T |
5: 134,962,390 (GRCm39) |
R63W |
possibly damaging |
Het |
| Nap1l1 |
C |
T |
10: 111,325,911 (GRCm39) |
R77* |
probably null |
Het |
| Niban2 |
T |
C |
2: 32,795,868 (GRCm39) |
I48T |
probably damaging |
Het |
| Notch4 |
G |
T |
17: 34,792,883 (GRCm39) |
|
probably null |
Het |
| Npepps |
A |
G |
11: 97,113,983 (GRCm39) |
I631T |
probably benign |
Het |
| Or4c105 |
G |
A |
2: 88,647,958 (GRCm39) |
V148I |
probably benign |
Het |
| Or8b51 |
A |
T |
9: 38,568,964 (GRCm39) |
C241* |
probably null |
Het |
| Pacsin3 |
T |
A |
2: 91,093,138 (GRCm39) |
L210Q |
probably damaging |
Het |
| Ppp2r1a |
A |
T |
17: 21,181,855 (GRCm39) |
I458F |
probably damaging |
Het |
| Ryr2 |
T |
C |
13: 11,609,785 (GRCm39) |
E4347G |
probably benign |
Het |
| Ryr3 |
A |
G |
2: 112,657,048 (GRCm39) |
I1911T |
probably damaging |
Het |
| Sec16a |
A |
G |
2: 26,329,417 (GRCm39) |
I866T |
possibly damaging |
Het |
| Sfr1 |
G |
T |
19: 47,722,019 (GRCm39) |
C145F |
probably benign |
Het |
| Slc35f4 |
A |
T |
14: 49,551,175 (GRCm39) |
Y230N |
probably damaging |
Het |
| Slx4 |
A |
G |
16: 3,818,849 (GRCm39) |
V89A |
|
Het |
| Son |
AGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCG |
AGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCG |
16: 91,457,222 (GRCm39) |
|
probably benign |
Het |
| Tbc1d23 |
A |
G |
16: 56,990,765 (GRCm39) |
I671T |
probably damaging |
Het |
| Trub1 |
G |
A |
19: 57,472,075 (GRCm39) |
V184I |
probably benign |
Het |
| Usp10 |
CAA |
CAAA |
8: 120,658,620 (GRCm39) |
|
probably null |
Het |
| Vit |
A |
C |
17: 78,932,398 (GRCm39) |
T502P |
probably damaging |
Het |
| Vmn2r15 |
C |
T |
5: 109,434,923 (GRCm39) |
G594R |
probably damaging |
Het |
|
| Other mutations in Mettl25 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00310:Mettl25
|
APN |
10 |
105,662,295 (GRCm39) |
missense |
probably benign |
|
|
IGL00698:Mettl25
|
APN |
10 |
105,629,201 (GRCm39) |
missense |
probably null |
0.31 |
|
IGL00766:Mettl25
|
APN |
10 |
105,615,443 (GRCm39) |
splice site |
probably benign |
|
|
IGL01360:Mettl25
|
APN |
10 |
105,659,058 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01954:Mettl25
|
APN |
10 |
105,659,068 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02088:Mettl25
|
APN |
10 |
105,659,111 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02623:Mettl25
|
APN |
10 |
105,662,185 (GRCm39) |
missense |
probably damaging |
0.96 |
|
IGL03245:Mettl25
|
APN |
10 |
105,662,358 (GRCm39) |
missense |
possibly damaging |
0.86 |
|
IGL03134:Mettl25
|
UTSW |
10 |
105,661,888 (GRCm39) |
nonsense |
probably null |
|
|
R0238:Mettl25
|
UTSW |
10 |
105,662,386 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0238:Mettl25
|
UTSW |
10 |
105,662,386 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0239:Mettl25
|
UTSW |
10 |
105,662,386 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0239:Mettl25
|
UTSW |
10 |
105,662,386 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1297:Mettl25
|
UTSW |
10 |
105,659,126 (GRCm39) |
missense |
probably benign |
0.11 |
|
R1482:Mettl25
|
UTSW |
10 |
105,662,451 (GRCm39) |
missense |
possibly damaging |
0.61 |
|
R1526:Mettl25
|
UTSW |
10 |
105,668,844 (GRCm39) |
missense |
possibly damaging |
0.56 |
|
R1542:Mettl25
|
UTSW |
10 |
105,661,981 (GRCm39) |
missense |
probably benign |
0.04 |
|
R1589:Mettl25
|
UTSW |
10 |
105,615,493 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1901:Mettl25
|
UTSW |
10 |
105,661,948 (GRCm39) |
missense |
probably damaging |
0.96 |
|
R1902:Mettl25
|
UTSW |
10 |
105,661,948 (GRCm39) |
missense |
probably damaging |
0.96 |
|
R2016:Mettl25
|
UTSW |
10 |
105,633,167 (GRCm39) |
missense |
probably benign |
0.00 |
|
R2355:Mettl25
|
UTSW |
10 |
105,599,316 (GRCm39) |
missense |
probably benign |
0.00 |
|
R2920:Mettl25
|
UTSW |
10 |
105,601,038 (GRCm39) |
splice site |
probably null |
|
|
R3149:Mettl25
|
UTSW |
10 |
105,662,214 (GRCm39) |
missense |
probably benign |
0.00 |
|
R4547:Mettl25
|
UTSW |
10 |
105,661,878 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4646:Mettl25
|
UTSW |
10 |
105,662,416 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4652:Mettl25
|
UTSW |
10 |
105,615,509 (GRCm39) |
nonsense |
probably null |
|
|
R5020:Mettl25
|
UTSW |
10 |
105,662,068 (GRCm39) |
missense |
possibly damaging |
0.87 |
|
R5435:Mettl25
|
UTSW |
10 |
105,615,447 (GRCm39) |
critical splice donor site |
probably null |
|
|
R6947:Mettl25
|
UTSW |
10 |
105,662,053 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7075:Mettl25
|
UTSW |
10 |
105,665,785 (GRCm39) |
missense |
possibly damaging |
0.68 |
|
R7729:Mettl25
|
UTSW |
10 |
105,601,871 (GRCm39) |
missense |
probably benign |
0.03 |
|
R8074:Mettl25
|
UTSW |
10 |
105,661,941 (GRCm39) |
missense |
probably benign |
0.02 |
|
R8108:Mettl25
|
UTSW |
10 |
105,659,040 (GRCm39) |
missense |
possibly damaging |
0.53 |
|
R8415:Mettl25
|
UTSW |
10 |
105,662,002 (GRCm39) |
missense |
probably benign |
0.05 |
|
R8937:Mettl25
|
UTSW |
10 |
105,601,122 (GRCm39) |
missense |
probably benign |
0.17 |
|
R8949:Mettl25
|
UTSW |
10 |
105,668,714 (GRCm39) |
missense |
probably benign |
0.00 |
|
R9497:Mettl25
|
UTSW |
10 |
105,677,090 (GRCm39) |
missense |
probably damaging |
0.98 |
|
RF009:Mettl25
|
UTSW |
10 |
105,669,100 (GRCm39) |
intron |
probably benign |
|
|
Z1176:Mettl25
|
UTSW |
10 |
105,661,959 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
| Predicted Primers |
PCR Primer
(F):5'- GCTCTCTTCACGTGGCTATG -3'
(R):5'- GCACTGTTAAACATCTTGTCACCAC -3'
Sequencing Primer
(F):5'- TTCCCTATCTGAAACGTAGGAAGAC -3'
(R):5'- AAACATCTTGTCACCACTTCATTTTC -3'
|
| Posted On |
2022-11-14 |
Incidental Mutation