Mutagenetix > Incidental Mutation

Incidental Mutation 'R9776:Zmym4'

733686

Institutional Source

Beutler Lab

Gene Symbol

Zmym4

Ensembl Gene

ENSMUSG00000042446

Gene Name

zinc finger, MYM-type 4

Synonyms

Zfp262, 6330503C17Rik

MMRRC Submission

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.513) question?

Stock #

R9776 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

126755732-126861928 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 126804942 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Arginine at position 439 (S439R)

Ref Sequence

ENSEMBL: ENSMUSP00000101714 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000106108]

AlphaFold

A2A791

Predicted Effect

possibly damaging
Transcript: ENSMUST00000106108
AA Change: S439R

PolyPhen 2 Score 0.510 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000101714
Gene: ENSMUSG00000042446
AA Change: S439R

Domain	Start	End	E-Value	Type
TRASH	341	377	6.53e-4	SMART
TRASH	389	429	7.22e-6	SMART
TRASH	441	479	1.77e0	SMART
TRASH	486	525	4.95e-4	SMART
TRASH	531	569	1.05e-2	SMART
TRASH	579	615	2.82e1	SMART
low complexity region	640	649	N/A	INTRINSIC
TRASH	687	723	8.49e-3	SMART
TRASH	729	764	1.14e-3	SMART
TRASH	772	810	4.48e-2	SMART
TRASH	816	851	2.06e-1	SMART
low complexity region	974	993	N/A	INTRINSIC
low complexity region	1002	1021	N/A	INTRINSIC
Pfam:DUF3504	1357	1527	1.7e-68	PFAM

Predicted Effect

SMART Domains

Protein: ENSMUSP00000120813
Gene: ENSMUSG00000042446
AA Change: S187R

Domain	Start	End	E-Value	Type
TRASH	90	126	6.53e-4	SMART
TRASH	138	178	7.22e-6	SMART
TRASH	190	228	1.77e0	SMART
TRASH	235	274	3.05e-4	SMART
low complexity region	300	309	N/A	INTRINSIC
TRASH	347	383	8.49e-3	SMART
TRASH	389	424	1.14e-3	SMART
TRASH	432	470	4.48e-2	SMART
TRASH	476	511	2.06e-1	SMART
low complexity region	634	653	N/A	INTRINSIC
low complexity region	662	681	N/A	INTRINSIC
Pfam:DUF3504	1017	1187	1.5e-68	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.8%
10x: 99.4%
20x: 98.5%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 83 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700069L16Rik	T	G	5: 113,861,765 (GRCm39)	Q35P	unknown	Het
2010109A12Rik	T	A	5: 93,361,305 (GRCm39)	*120K	probably null	Het
2700049A03Rik	A	G	12: 71,235,448 (GRCm39)	T1133A	possibly damaging	Het
A830005F24Rik	G	A	13: 48,667,758 (GRCm39)	R20Q	unknown	Het
Acta2	T	C	19: 34,223,481 (GRCm39)	T205A	probably benign	Het
Adcy5	A	G	16: 35,100,725 (GRCm39)	D759G	probably damaging	Het
Ankk1	T	A	9: 49,330,714 (GRCm39)	M276L	probably benign	Het
Arfgef2	G	A	2: 166,713,447 (GRCm39)	E1283K	probably damaging	Het
Arhgef26	G	T	3: 62,246,803 (GRCm39)		probably benign	Het
Arhgef28	A	C	13: 98,133,415 (GRCm39)	S351A	probably benign	Het
Arih2	C	T	9: 108,484,504 (GRCm39)	G436S	probably damaging	Het
Bptf	T	C	11: 106,969,396 (GRCm39)	T1013A	probably damaging	Het
Camsap1	A	T	2: 25,828,166 (GRCm39)	V1186E	probably benign	Het
Capn10	G	T	1: 92,871,586 (GRCm39)	A395S	possibly damaging	Het
Ccdc184	A	T	15: 98,066,737 (GRCm39)	T181S	probably damaging	Het
Clasp1	T	C	1: 118,509,108 (GRCm39)	I1345T	possibly damaging	Het
Cpsf1	A	T	15: 76,486,779 (GRCm39)	H252Q	probably damaging	Het
Ctnna2	T	A	6: 77,582,172 (GRCm39)	M363L	probably benign	Het
Cyp24a1	G	A	2: 170,338,625 (GRCm39)	P24S	probably benign	Het
D130040H23Rik	A	G	8: 69,755,566 (GRCm39)	H341R	probably damaging	Het
Dclk3	A	G	9: 111,298,226 (GRCm39)	E590G	probably damaging	Het
Ear2	A	T	14: 44,340,729 (GRCm39)	Y129F	probably benign	Het
Egf	A	G	3: 129,530,514 (GRCm39)	L216P	probably damaging	Het
Fbxw9	T	A	8: 85,792,523 (GRCm39)	H319Q	probably damaging	Het
Gipr	A	G	7: 18,891,487 (GRCm39)	S397P	probably damaging	Het
Gxylt2	G	T	6: 100,682,072 (GRCm39)	E90*	probably null	Het
Hnf1b	T	C	11: 83,784,283 (GRCm39)	V473A	probably benign	Het
Iffo1	G	A	6: 125,130,436 (GRCm39)	E475K	probably damaging	Het
Ifi206	T	A	1: 173,308,075 (GRCm39)	R640S		Het
Ipmk	A	G	10: 71,217,439 (GRCm39)	S328G	probably damaging	Het
Kcnq1	A	T	7: 142,737,368 (GRCm39)	I229F	probably damaging	Het
Kif9	C	A	9: 110,350,398 (GRCm39)	T763N	probably benign	Het
Klhl36	C	T	8: 120,601,129 (GRCm39)	Q383*	probably null	Het
Lrp4	G	T	2: 91,316,179 (GRCm39)	V766F	probably damaging	Het
Mettl26	T	A	17: 26,094,511 (GRCm39)	M3K	probably benign	Het
Muc16	T	A	9: 18,570,675 (GRCm39)	I615F	unknown	Het
Myo7b	T	A	18: 32,133,068 (GRCm39)	Q427L	probably benign	Het
Naip1	A	G	13: 100,559,584 (GRCm39)	M1140T	probably benign	Het
Ncapg	G	A	5: 45,829,834 (GRCm39)	V179I	probably damaging	Het
Nme4	C	T	17: 26,314,410 (GRCm39)	G6E	possibly damaging	Het
Nr1h4	T	C	10: 89,319,311 (GRCm39)	Y185C	probably damaging	Het
Nt5c3b	T	C	11: 100,327,012 (GRCm39)	I95V	probably benign	Het
Ntaq1	A	G	15: 58,004,913 (GRCm39)	T9A	probably benign	Het
Or2y3	C	T	17: 38,393,470 (GRCm39)	R133H	probably benign	Het
Or5g9	A	T	2: 85,552,145 (GRCm39)	Y132F	probably damaging	Het
Or5t16	A	T	2: 86,819,055 (GRCm39)	I155N	probably damaging	Het
Pappa2	G	T	1: 158,611,481 (GRCm39)	P1494Q	probably damaging	Het
Pdzd2	A	G	15: 12,457,909 (GRCm39)	F144S	probably benign	Het
Phactr3	A	G	2: 177,975,896 (GRCm39)	H547R	probably damaging	Het
Pik3c2b	T	A	1: 133,018,588 (GRCm39)	S1012T	possibly damaging	Het
Plppr2	G	A	9: 21,859,107 (GRCm39)	G408D	probably damaging	Het
Pom121	T	A	5: 135,420,554 (GRCm39)	N289I	unknown	Het
Ppa1	T	A	10: 61,487,362 (GRCm39)	S30T	probably damaging	Het
Rgs5	A	T	1: 169,518,089 (GRCm39)	K108*	probably null	Het
Rpap1	A	G	2: 119,607,278 (GRCm39)	V287A	probably benign	Het
Ryr1	A	G	7: 28,774,664 (GRCm39)	Y2326H	probably damaging	Het
Ryr2	A	G	13: 11,707,599 (GRCm39)	S2813P	probably damaging	Het
Sap25	T	C	5: 137,640,702 (GRCm39)		probably null	Het
Senp5	T	C	16: 31,782,279 (GRCm39)	Y739C	probably damaging	Het
Slc25a29	A	T	12: 108,793,017 (GRCm39)	L187Q	possibly damaging	Het
Slc7a2	C	T	8: 41,358,641 (GRCm39)	T328M	probably damaging	Het
Slc7a8	T	C	14: 55,018,759 (GRCm39)	N9S	probably benign	Het
Snx18	A	G	13: 113,754,039 (GRCm39)	V298A	probably benign	Het
Spo11	T	A	2: 172,833,904 (GRCm39)	I342N	possibly damaging	Het
Ssc5d	A	G	7: 4,932,367 (GRCm39)	K344E	probably benign	Het
Sspo	A	T	6: 48,439,269 (GRCm39)	D1639V	probably benign	Het
Stk32b	T	C	5: 37,617,001 (GRCm39)	D285G	probably benign	Het
Sult1b1	T	C	5: 87,662,815 (GRCm39)	D295G	probably benign	Het
Syt5	A	G	7: 4,544,831 (GRCm39)	L274P	probably damaging	Het
Tas1r2	T	C	4: 139,396,208 (GRCm39)	S545P	possibly damaging	Het
Tbc1d17	T	G	7: 44,490,696 (GRCm39)	D632A	probably damaging	Het
Tbc1d2	A	G	4: 46,650,007 (GRCm39)	S10P	probably benign	Het
Tlk1	A	T	2: 70,555,908 (GRCm39)	I417N	probably damaging	Het
Tmem184a	C	T	5: 139,791,984 (GRCm39)	R348H	possibly damaging	Het
Togaram2	T	A	17: 72,023,508 (GRCm39)	V808D	possibly damaging	Het
Tpr	A	G	1: 150,324,939 (GRCm39)	Q2397R	probably benign	Het
Trav7-4	A	G	14: 53,698,994 (GRCm39)	D47G	possibly damaging	Het
Usp17le	T	A	7: 104,419,814 (GRCm39)	M35L	probably benign	Het
Vegfc	C	T	8: 54,633,829 (GRCm39)	S262L	probably benign	Het
Vmn2r74	T	A	7: 85,605,212 (GRCm39)	I479L	possibly damaging	Het
Vps13a	A	T	19: 16,736,958 (GRCm39)	F126L	probably benign	Het
Zfp945	C	A	17: 23,070,582 (GRCm39)	C460F	possibly damaging	Het
Zscan4-ps3	A	G	7: 11,344,093 (GRCm39)	E17G	probably damaging	Het

Other mutations in Zmym4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00537:Zmym4	APN	4	126,783,851 (GRCm39)	missense	probably benign
IGL00845:Zmym4	APN	4	126,794,406 (GRCm39)	missense	probably benign	0.28
IGL01122:Zmym4	APN	4	126,758,045 (GRCm39)	missense	probably damaging	1.00
IGL01374:Zmym4	APN	4	126,762,750 (GRCm39)	missense	probably damaging	1.00
IGL01564:Zmym4	APN	4	126,805,073 (GRCm39)	missense	possibly damaging	0.84
IGL02014:Zmym4	APN	4	126,794,462 (GRCm39)	missense	possibly damaging	0.67
IGL02187:Zmym4	APN	4	126,764,066 (GRCm39)	missense	probably damaging	0.97
IGL02887:Zmym4	APN	4	126,842,268 (GRCm39)	missense	probably damaging	0.96
IGL03371:Zmym4	APN	4	126,808,881 (GRCm39)	missense	possibly damaging	0.90
IGL03400:Zmym4	APN	4	126,816,920 (GRCm39)	missense	probably benign	0.12
arriba	UTSW	4	126,809,415 (GRCm39)	missense	probably damaging	0.97
foreclosed	UTSW	4	126,789,099 (GRCm39)	missense	probably damaging	1.00
Foreshortened	UTSW	4	126,804,729 (GRCm39)	missense	possibly damaging	0.75
levantese	UTSW	4	126,757,961 (GRCm39)	missense	probably damaging	0.99
terminated	UTSW	4	126,819,588 (GRCm39)	missense	probably benign	0.02
BB004:Zmym4	UTSW	4	126,799,170 (GRCm39)	missense	probably benign	0.42
BB014:Zmym4	UTSW	4	126,799,170 (GRCm39)	missense	probably benign	0.42
R0149:Zmym4	UTSW	4	126,804,938 (GRCm39)	missense	probably benign	0.00
R0361:Zmym4	UTSW	4	126,804,938 (GRCm39)	missense	probably benign	0.00
R0423:Zmym4	UTSW	4	126,776,112 (GRCm39)	splice site	probably benign
R0532:Zmym4	UTSW	4	126,792,194 (GRCm39)	nonsense	probably null
R0745:Zmym4	UTSW	4	126,796,496 (GRCm39)	splice site	probably benign
R1183:Zmym4	UTSW	4	126,819,632 (GRCm39)	missense	probably damaging	0.99
R1401:Zmym4	UTSW	4	126,804,962 (GRCm39)	missense	probably benign	0.00
R1446:Zmym4	UTSW	4	126,776,275 (GRCm39)	missense	probably damaging	1.00
R1491:Zmym4	UTSW	4	126,776,105 (GRCm39)	critical splice acceptor site	probably null
R1566:Zmym4	UTSW	4	126,804,940 (GRCm39)	missense	possibly damaging	0.94
R1962:Zmym4	UTSW	4	126,796,463 (GRCm39)	missense	possibly damaging	0.47
R2398:Zmym4	UTSW	4	126,816,929 (GRCm39)	missense	probably damaging	1.00
R2930:Zmym4	UTSW	4	126,819,316 (GRCm39)	missense	probably benign	0.00
R3891:Zmym4	UTSW	4	126,798,269 (GRCm39)	missense	probably benign	0.04
R3892:Zmym4	UTSW	4	126,798,269 (GRCm39)	missense	probably benign	0.04
R4659:Zmym4	UTSW	4	126,842,221 (GRCm39)	splice site	probably null
R4702:Zmym4	UTSW	4	126,816,958 (GRCm39)	missense	probably benign	0.01
R5160:Zmym4	UTSW	4	126,763,977 (GRCm39)	missense	probably damaging	0.97
R5614:Zmym4	UTSW	4	126,804,729 (GRCm39)	missense	possibly damaging	0.75
R5773:Zmym4	UTSW	4	126,799,163 (GRCm39)	missense	possibly damaging	0.52
R6450:Zmym4	UTSW	4	126,789,099 (GRCm39)	missense	probably damaging	1.00
R6741:Zmym4	UTSW	4	126,808,878 (GRCm39)	missense	possibly damaging	0.80
R7023:Zmym4	UTSW	4	126,762,593 (GRCm39)	missense	probably damaging	1.00
R7315:Zmym4	UTSW	4	126,776,385 (GRCm39)	missense	probably benign	0.09
R7468:Zmym4	UTSW	4	126,776,029 (GRCm39)	missense	probably benign	0.40
R7546:Zmym4	UTSW	4	126,757,961 (GRCm39)	missense	probably damaging	0.99
R7609:Zmym4	UTSW	4	126,819,588 (GRCm39)	missense	probably benign	0.02
R7764:Zmym4	UTSW	4	126,819,409 (GRCm39)	missense	probably benign	0.06
R7897:Zmym4	UTSW	4	126,783,332 (GRCm39)	missense	possibly damaging	0.76
R7918:Zmym4	UTSW	4	126,804,797 (GRCm39)	critical splice acceptor site	probably null
R7927:Zmym4	UTSW	4	126,799,170 (GRCm39)	missense	probably benign	0.42
R8129:Zmym4	UTSW	4	126,808,956 (GRCm39)	missense	possibly damaging	0.87
R8240:Zmym4	UTSW	4	126,798,188 (GRCm39)	critical splice donor site	probably null
R8248:Zmym4	UTSW	4	126,799,162 (GRCm39)	missense	possibly damaging	0.56
R8261:Zmym4	UTSW	4	126,798,360 (GRCm39)	missense	probably damaging	1.00
R8313:Zmym4	UTSW	4	126,804,762 (GRCm39)	missense	probably benign	0.19
R8353:Zmym4	UTSW	4	126,800,905 (GRCm39)	missense	possibly damaging	0.46
R8747:Zmym4	UTSW	4	126,787,198 (GRCm39)	missense	probably damaging	1.00
R8787:Zmym4	UTSW	4	126,816,953 (GRCm39)	missense	probably benign	0.41
R8795:Zmym4	UTSW	4	126,799,819 (GRCm39)	missense	probably benign	0.35
R8948:Zmym4	UTSW	4	126,758,060 (GRCm39)	missense	probably damaging	1.00
R9218:Zmym4	UTSW	4	126,809,415 (GRCm39)	missense	probably damaging	0.97
R9233:Zmym4	UTSW	4	126,776,310 (GRCm39)	missense	probably damaging	0.99
R9286:Zmym4	UTSW	4	126,783,812 (GRCm39)	missense	probably damaging	1.00
R9468:Zmym4	UTSW	4	126,800,993 (GRCm39)	missense	probably benign	0.01
R9542:Zmym4	UTSW	4	126,799,164 (GRCm39)	missense	probably benign	0.00
R9756:Zmym4	UTSW	4	126,771,502 (GRCm39)	missense	probably damaging	1.00
U24488:Zmym4	UTSW	4	126,819,453 (GRCm39)	missense	possibly damaging	0.69
Z1177:Zmym4	UTSW	4	126,801,005 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- TCACTGCAGAGCTTATGAACC -3'
(R):5'- TGGTTCCTTTAATATACCAAGAGGG -3'

Sequencing Primer
(F):5'- GCAGAGCTTATGAACCACGTTCTG -3'
(R):5'- GGTGGTAAATGTAATATCCTGTGAC -3'

Posted On

2022-11-14