Mutagenetix > Incidental Mutation

Incidental Mutation 'R9778:Zfp949'

733851

Institutional Source

Beutler Lab

Gene Symbol

Zfp949

Ensembl Gene

ENSMUSG00000032425

Gene Name

zinc finger protein 949

Synonyms

4930422I07Rik, Nczf

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.057) question?

Stock #

R9778 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

88430073-88453114 bp(+) (GRCm39)

Type of Mutation

critical splice donor site (2 bp from exon)

DNA Base Change (assembly)

T to C at 88449340 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000125325 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000160652] [ENSMUST00000161458] [ENSMUST00000162827]

AlphaFold

E9Q732

Predicted Effect

probably benign
Transcript: ENSMUST00000160652

Predicted Effect

probably null
Transcript: ENSMUST00000161458

SMART Domains

Protein: ENSMUSP00000125017
Gene: ENSMUSG00000032425

Domain	Start	End	E-Value	Type
KRAB	8	68	2.63e-32	SMART
ZnF_C2H2	268	290	5.99e1	SMART
ZnF_C2H2	296	318	5.5e-3	SMART
ZnF_C2H2	324	346	6.42e-4	SMART
ZnF_C2H2	352	374	2.91e-2	SMART
ZnF_C2H2	380	402	4.11e-2	SMART
ZnF_C2H2	408	430	3.63e-3	SMART
ZnF_C2H2	436	458	5.67e-5	SMART
ZnF_C2H2	464	486	7.9e-4	SMART
ZnF_C2H2	492	514	2.43e-4	SMART
ZnF_C2H2	520	542	2.95e-3	SMART
ZnF_C2H2	548	570	1.03e-2	SMART
ZnF_C2H2	576	598	1.4e-4	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000162827

SMART Domains

Protein: ENSMUSP00000125325
Gene: ENSMUSG00000032425

Domain	Start	End	E-Value	Type
KRAB	8	68	2.63e-32	SMART
ZnF_C2H2	268	290	5.99e1	SMART
ZnF_C2H2	296	318	5.5e-3	SMART
ZnF_C2H2	324	346	6.42e-4	SMART
ZnF_C2H2	352	374	2.91e-2	SMART
ZnF_C2H2	380	402	4.11e-2	SMART
ZnF_C2H2	408	430	3.63e-3	SMART
ZnF_C2H2	436	458	5.67e-5	SMART
ZnF_C2H2	464	486	7.9e-4	SMART
ZnF_C2H2	492	514	2.43e-4	SMART
ZnF_C2H2	520	542	2.95e-3	SMART
ZnF_C2H2	548	570	1.03e-2	SMART
ZnF_C2H2	576	598	1.4e-4	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000162985

SMART Domains

Protein: ENSMUSP00000124007
Gene: ENSMUSG00000032425

Domain	Start	End	E-Value	Type
KRAB	8	68	2.63e-32	SMART

Meta Mutation Damage Score

0.9756

Coding Region Coverage

1x: 99.9%
3x: 99.8%
10x: 99.4%
20x: 98.5%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit embryonic lethality during organogenesis with defects in growth, development, cell proliferation, apoptosis and turning. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310022B05Rik	A	T	8: 125,366,098 (GRCm39)	I215N	probably damaging	Het
Aig1	T	C	10: 13,528,757 (GRCm39)		probably null	Het
Atp8b2	T	A	3: 89,861,865 (GRCm39)	K82N	possibly damaging	Het
Cacnb4	T	G	2: 52,359,615 (GRCm39)	Y131S	probably damaging	Het
Cep170b	G	A	12: 112,697,864 (GRCm39)	S62N	possibly damaging	Het
Crmp1	A	G	5: 37,422,619 (GRCm39)	D142G	probably benign	Het
Csf1r	A	G	18: 61,260,957 (GRCm39)	Q716R	possibly damaging	Het
Cyp2u1	T	C	3: 131,087,133 (GRCm39)	T483A	possibly damaging	Het
D930020B18Rik	T	C	10: 121,503,565 (GRCm39)	F247L	probably benign	Het
Dcaf13	T	C	15: 39,008,586 (GRCm39)	L396P	probably damaging	Het
Dnhd1	A	G	7: 105,353,240 (GRCm39)	M2798V	probably benign	Het
E030018B13Rik	A	T	7: 63,569,125 (GRCm39)	T58S	unknown	Het
Eipr1	T	C	12: 28,897,657 (GRCm39)		probably null	Het
Elf1	C	T	14: 79,817,948 (GRCm39)	T526M	possibly damaging	Het
Gm8369	GTGTGTGTGTGTGTGTGTGTG	GTGTGTGTGTGTGTGTGTGTGTG	19: 11,489,128 (GRCm39)		probably null	Het
Got1	A	G	19: 43,504,284 (GRCm39)	S46P	probably benign	Het
Inpp4b	T	C	8: 82,775,160 (GRCm39)	I786T	probably benign	Het
Kdm1a	T	C	4: 136,279,892 (GRCm39)	I703M	probably damaging	Het
Krt222	A	T	11: 99,125,838 (GRCm39)	S264R	probably benign	Het
Lrrd1	A	C	5: 3,899,982 (GRCm39)	S96R	possibly damaging	Het
Ltb	C	A	17: 35,414,906 (GRCm39)	Y235*	probably null	Het
Mbd2	T	A	18: 70,751,050 (GRCm39)	C362S	probably damaging	Het
Mlycd	T	A	8: 120,129,325 (GRCm39)	I264N	probably damaging	Het
Mri1	C	T	8: 84,980,933 (GRCm39)	C199Y	possibly damaging	Het
Muc5ac	A	T	7: 141,349,021 (GRCm39)	K487*	probably null	Het
Myh13	T	C	11: 67,249,016 (GRCm39)	I1274T	probably damaging	Het
Ncdn	G	A	4: 126,642,467 (GRCm39)	R397W	probably damaging	Het
Ncf1	T	A	5: 134,258,444 (GRCm39)		probably benign	Het
Nipbl	C	A	15: 8,321,032 (GRCm39)	A2692S	probably benign	Het
Odf2l	C	T	3: 144,854,789 (GRCm39)	T542I	possibly damaging	Het
Or10a49	A	G	7: 108,467,698 (GRCm39)	I221T	probably damaging	Het
Or10al2	T	A	17: 37,983,145 (GRCm39)	I77N	probably damaging	Het
Oxsm	T	C	14: 16,242,629 (GRCm38)	T47A	possibly damaging	Het
Pcdhga3	A	T	18: 37,807,786 (GRCm39)	S80C	probably benign	Het
Pcnx2	T	C	8: 126,512,176 (GRCm39)	T1377A	probably benign	Het
Phactr3	C	A	2: 177,924,805 (GRCm39)	Q252K	possibly damaging	Het
Phf2	T	C	13: 48,973,101 (GRCm39)	H385R	unknown	Het
Pkd1l3	A	T	8: 110,357,937 (GRCm39)	T820S	probably benign	Het
Plekhg1	T	A	10: 3,887,966 (GRCm39)	H353Q		Het
Prss37	T	A	6: 40,494,713 (GRCm39)	E24V	probably damaging	Het
Reln	A	G	5: 22,155,943 (GRCm39)	F2183S	probably damaging	Het
Slc20a2	A	G	8: 23,051,407 (GRCm39)	D480G	probably damaging	Het
Slc8a2	C	T	7: 15,887,124 (GRCm39)	T672M	probably damaging	Het
Slf2	A	G	19: 44,961,666 (GRCm39)	T1062A	probably benign	Het
Snx29	A	G	16: 11,223,609 (GRCm39)	E18G	possibly damaging	Het
Spata31e1	C	T	13: 49,939,542 (GRCm39)	V723M	possibly damaging	Het
Srf	C	A	17: 46,860,079 (GRCm39)	G482V	possibly damaging	Het
Styk1	G	A	6: 131,287,992 (GRCm39)	Q124*	probably null	Het
Tcf7l1	G	T	6: 72,608,226 (GRCm39)	A385E	probably damaging	Het
Thbs4	A	G	13: 92,913,495 (GRCm39)	S142P	probably benign	Het
Tlr1	G	A	5: 65,083,371 (GRCm39)	S402F	probably damaging	Het
Tnrc6a	A	G	7: 122,769,635 (GRCm39)	H475R	probably benign	Het
Trmt10b	A	T	4: 45,314,374 (GRCm39)	N272I	probably damaging	Het
Ubxn7	A	T	16: 32,200,471 (GRCm39)	N409I	probably benign	Het
Vcan	T	C	13: 89,837,930 (GRCm39)	E2538G	probably damaging	Het
Vmn1r65	C	T	7: 6,011,387 (GRCm39)	W282*	probably null	Het
Vmn2r4	A	G	3: 64,322,497 (GRCm39)	I74T	probably benign	Het
Wdr97	T	A	15: 76,241,125 (GRCm39)	F574I		Het

Other mutations in Zfp949

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03201:Zfp949	APN	9	88,450,717 (GRCm39)	missense	probably benign	0.23
R0034:Zfp949	UTSW	9	88,449,693 (GRCm39)	intron	probably benign
R0462:Zfp949	UTSW	9	88,450,787 (GRCm39)	missense	possibly damaging	0.63
R1457:Zfp949	UTSW	9	88,451,891 (GRCm39)	missense	probably damaging	1.00
R1574:Zfp949	UTSW	9	88,451,830 (GRCm39)	nonsense	probably null
R1574:Zfp949	UTSW	9	88,451,830 (GRCm39)	nonsense	probably null
R1878:Zfp949	UTSW	9	88,451,356 (GRCm39)	missense	probably damaging	0.99
R1917:Zfp949	UTSW	9	88,452,115 (GRCm39)	missense	probably damaging	0.98
R4488:Zfp949	UTSW	9	88,452,142 (GRCm39)	missense	probably damaging	0.98
R4839:Zfp949	UTSW	9	88,452,047 (GRCm39)	missense	probably damaging	0.97
R5309:Zfp949	UTSW	9	88,449,236 (GRCm39)	missense	possibly damaging	0.92
R5312:Zfp949	UTSW	9	88,449,236 (GRCm39)	missense	possibly damaging	0.92
R5461:Zfp949	UTSW	9	88,451,537 (GRCm39)	missense	probably benign	0.00
R6530:Zfp949	UTSW	9	88,449,340 (GRCm39)	critical splice donor site	probably null
R6844:Zfp949	UTSW	9	88,451,464 (GRCm39)	missense	possibly damaging	0.91
R7749:Zfp949	UTSW	9	88,451,923 (GRCm39)	missense	probably damaging	1.00
R7937:Zfp949	UTSW	9	88,451,323 (GRCm39)	missense	probably damaging	1.00
R8150:Zfp949	UTSW	9	88,452,053 (GRCm39)	missense	probably benign
R8290:Zfp949	UTSW	9	88,451,293 (GRCm39)	missense	probably damaging	0.98
R8349:Zfp949	UTSW	9	88,449,302 (GRCm39)	missense	possibly damaging	0.84
R8449:Zfp949	UTSW	9	88,449,302 (GRCm39)	missense	possibly damaging	0.84
R8808:Zfp949	UTSW	9	88,451,417 (GRCm39)	missense	probably damaging	1.00
R8949:Zfp949	UTSW	9	88,450,771 (GRCm39)	missense	possibly damaging	0.78
R9219:Zfp949	UTSW	9	88,451,723 (GRCm39)	missense	probably damaging	1.00
R9396:Zfp949	UTSW	9	88,449,260 (GRCm39)	missense	probably damaging	0.99
R9486:Zfp949	UTSW	9	88,452,182 (GRCm39)	missense	probably benign	0.01
R9488:Zfp949	UTSW	9	88,452,182 (GRCm39)	missense	probably benign	0.01
R9643:Zfp949	UTSW	9	88,436,500 (GRCm39)	start gained	probably benign
R9727:Zfp949	UTSW	9	88,451,913 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- CAAGCCTAGAGTAAATTGATTGAGG -3'
(R):5'- GGGATGCATTGCCCTGTTAATAG -3'

Sequencing Primer
(F):5'- ATTGAGGAGAAATGTGCTGTTAC -3'
(R):5'- TTTGCTTAAATGTTCTTTGGAGAAAG -3'

Posted On

2022-11-14