Mutagenetix > Incidental Mutation

Incidental Mutation 'R9778:Or10al2'

733871

Institutional Source

Beutler Lab

Gene Symbol

Or10al2

Ensembl Gene

ENSMUSG00000080990

Gene Name

olfactory receptor family 10 subfamily AL member 2

Synonyms

GA_x6K02T2PSCP-2131124-2132089, MOR263-13, Olfr118

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.070) question?

Stock #

R9778 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

37982916-37983888 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 37983145 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Asparagine at position 77 (I77N)

Ref Sequence

ENSEMBL: ENSMUSP00000150176 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000122036] [ENSMUST00000215811] [ENSMUST00000216551]

AlphaFold

Q7TRJ6

Predicted Effect

probably damaging
Transcript: ENSMUST00000122036
AA Change: I77N

PolyPhen 2 Score 0.972 (Sensitivity: 0.77; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000113988
Gene: ENSMUSG00000080990
AA Change: I77N

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srv	32	173	2e-6	PFAM
Pfam:7tm_4	37	314	2.1e-57	PFAM
Pfam:7TM_GPCR_Srsx	41	311	5.8e-6	PFAM
Pfam:7tm_1	47	296	1.4e-24	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000215811
AA Change: I77N

PolyPhen 2 Score 0.972 (Sensitivity: 0.77; Specificity: 0.96)

Predicted Effect

probably damaging
Transcript: ENSMUST00000216551
AA Change: I77N

PolyPhen 2 Score 0.972 (Sensitivity: 0.77; Specificity: 0.96)

Coding Region Coverage

1x: 99.9%
3x: 99.8%
10x: 99.4%
20x: 98.5%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310022B05Rik	A	T	8: 125,366,098 (GRCm39)	I215N	probably damaging	Het
Aig1	T	C	10: 13,528,757 (GRCm39)		probably null	Het
Atp8b2	T	A	3: 89,861,865 (GRCm39)	K82N	possibly damaging	Het
Cacnb4	T	G	2: 52,359,615 (GRCm39)	Y131S	probably damaging	Het
Cep170b	G	A	12: 112,697,864 (GRCm39)	S62N	possibly damaging	Het
Crmp1	A	G	5: 37,422,619 (GRCm39)	D142G	probably benign	Het
Csf1r	A	G	18: 61,260,957 (GRCm39)	Q716R	possibly damaging	Het
Cyp2u1	T	C	3: 131,087,133 (GRCm39)	T483A	possibly damaging	Het
D930020B18Rik	T	C	10: 121,503,565 (GRCm39)	F247L	probably benign	Het
Dcaf13	T	C	15: 39,008,586 (GRCm39)	L396P	probably damaging	Het
Dnhd1	A	G	7: 105,353,240 (GRCm39)	M2798V	probably benign	Het
E030018B13Rik	A	T	7: 63,569,125 (GRCm39)	T58S	unknown	Het
Eipr1	T	C	12: 28,897,657 (GRCm39)		probably null	Het
Elf1	C	T	14: 79,817,948 (GRCm39)	T526M	possibly damaging	Het
Gm8369	GTGTGTGTGTGTGTGTGTGTG	GTGTGTGTGTGTGTGTGTGTGTG	19: 11,489,128 (GRCm39)		probably null	Het
Got1	A	G	19: 43,504,284 (GRCm39)	S46P	probably benign	Het
Inpp4b	T	C	8: 82,775,160 (GRCm39)	I786T	probably benign	Het
Kdm1a	T	C	4: 136,279,892 (GRCm39)	I703M	probably damaging	Het
Krt222	A	T	11: 99,125,838 (GRCm39)	S264R	probably benign	Het
Lrrd1	A	C	5: 3,899,982 (GRCm39)	S96R	possibly damaging	Het
Ltb	C	A	17: 35,414,906 (GRCm39)	Y235*	probably null	Het
Mbd2	T	A	18: 70,751,050 (GRCm39)	C362S	probably damaging	Het
Mlycd	T	A	8: 120,129,325 (GRCm39)	I264N	probably damaging	Het
Mri1	C	T	8: 84,980,933 (GRCm39)	C199Y	possibly damaging	Het
Muc5ac	A	T	7: 141,349,021 (GRCm39)	K487*	probably null	Het
Myh13	T	C	11: 67,249,016 (GRCm39)	I1274T	probably damaging	Het
Ncdn	G	A	4: 126,642,467 (GRCm39)	R397W	probably damaging	Het
Ncf1	T	A	5: 134,258,444 (GRCm39)		probably benign	Het
Nipbl	C	A	15: 8,321,032 (GRCm39)	A2692S	probably benign	Het
Odf2l	C	T	3: 144,854,789 (GRCm39)	T542I	possibly damaging	Het
Or10a49	A	G	7: 108,467,698 (GRCm39)	I221T	probably damaging	Het
Oxsm	T	C	14: 16,242,629 (GRCm38)	T47A	possibly damaging	Het
Pcdhga3	A	T	18: 37,807,786 (GRCm39)	S80C	probably benign	Het
Pcnx2	T	C	8: 126,512,176 (GRCm39)	T1377A	probably benign	Het
Phactr3	C	A	2: 177,924,805 (GRCm39)	Q252K	possibly damaging	Het
Phf2	T	C	13: 48,973,101 (GRCm39)	H385R	unknown	Het
Pkd1l3	A	T	8: 110,357,937 (GRCm39)	T820S	probably benign	Het
Plekhg1	T	A	10: 3,887,966 (GRCm39)	H353Q		Het
Prss37	T	A	6: 40,494,713 (GRCm39)	E24V	probably damaging	Het
Reln	A	G	5: 22,155,943 (GRCm39)	F2183S	probably damaging	Het
Slc20a2	A	G	8: 23,051,407 (GRCm39)	D480G	probably damaging	Het
Slc8a2	C	T	7: 15,887,124 (GRCm39)	T672M	probably damaging	Het
Slf2	A	G	19: 44,961,666 (GRCm39)	T1062A	probably benign	Het
Snx29	A	G	16: 11,223,609 (GRCm39)	E18G	possibly damaging	Het
Spata31e1	C	T	13: 49,939,542 (GRCm39)	V723M	possibly damaging	Het
Srf	C	A	17: 46,860,079 (GRCm39)	G482V	possibly damaging	Het
Styk1	G	A	6: 131,287,992 (GRCm39)	Q124*	probably null	Het
Tcf7l1	G	T	6: 72,608,226 (GRCm39)	A385E	probably damaging	Het
Thbs4	A	G	13: 92,913,495 (GRCm39)	S142P	probably benign	Het
Tlr1	G	A	5: 65,083,371 (GRCm39)	S402F	probably damaging	Het
Tnrc6a	A	G	7: 122,769,635 (GRCm39)	H475R	probably benign	Het
Trmt10b	A	T	4: 45,314,374 (GRCm39)	N272I	probably damaging	Het
Ubxn7	A	T	16: 32,200,471 (GRCm39)	N409I	probably benign	Het
Vcan	T	C	13: 89,837,930 (GRCm39)	E2538G	probably damaging	Het
Vmn1r65	C	T	7: 6,011,387 (GRCm39)	W282*	probably null	Het
Vmn2r4	A	G	3: 64,322,497 (GRCm39)	I74T	probably benign	Het
Wdr97	T	A	15: 76,241,125 (GRCm39)	F574I		Het
Zfp949	T	C	9: 88,449,340 (GRCm39)		probably null	Het

Other mutations in Or10al2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01300:Or10al2	APN	17	37,983,778 (GRCm39)	missense	probably damaging	1.00
IGL02456:Or10al2	APN	17	37,983,340 (GRCm39)	missense	possibly damaging	0.90
IGL02750:Or10al2	APN	17	37,983,500 (GRCm39)	nonsense	probably null
IGL03083:Or10al2	APN	17	37,983,551 (GRCm39)	nonsense	probably null
IGL03339:Or10al2	APN	17	37,983,448 (GRCm39)	missense	possibly damaging	0.87
R0032:Or10al2	UTSW	17	37,983,378 (GRCm39)	missense	probably damaging	1.00
R1457:Or10al2	UTSW	17	37,983,816 (GRCm39)	nonsense	probably null
R1542:Or10al2	UTSW	17	37,983,142 (GRCm39)	missense	probably damaging	1.00
R1771:Or10al2	UTSW	17	37,983,554 (GRCm39)	missense	probably damaging	1.00
R1893:Or10al2	UTSW	17	37,983,747 (GRCm39)	nonsense	probably null
R2395:Or10al2	UTSW	17	37,983,587 (GRCm39)	nonsense	probably null
R3619:Or10al2	UTSW	17	37,983,531 (GRCm39)	missense	probably benign	0.05
R3917:Or10al2	UTSW	17	37,983,684 (GRCm39)	missense	probably damaging	1.00
R3937:Or10al2	UTSW	17	37,983,858 (GRCm39)	missense	probably benign	0.01
R5600:Or10al2	UTSW	17	37,983,176 (GRCm39)	missense	possibly damaging	0.91
R6415:Or10al2	UTSW	17	37,983,448 (GRCm39)	missense	possibly damaging	0.87
R6462:Or10al2	UTSW	17	37,983,111 (GRCm39)	missense	probably damaging	1.00
R7355:Or10al2	UTSW	17	37,983,301 (GRCm39)	missense	probably benign	0.02
R7861:Or10al2	UTSW	17	37,983,408 (GRCm39)	missense	possibly damaging	0.91
R7913:Or10al2	UTSW	17	37,982,999 (GRCm39)	missense	probably benign
R7952:Or10al2	UTSW	17	37,983,708 (GRCm39)	missense	probably damaging	1.00
R7969:Or10al2	UTSW	17	37,983,547 (GRCm39)	missense	probably damaging	1.00
R8879:Or10al2	UTSW	17	37,983,302 (GRCm39)	nonsense	probably null
R8923:Or10al2	UTSW	17	37,983,702 (GRCm39)	missense	probably benign	0.00
R9253:Or10al2	UTSW	17	37,983,637 (GRCm39)	missense	probably benign	0.00
R9789:Or10al2	UTSW	17	37,983,059 (GRCm39)	missense	probably damaging	1.00
RF003:Or10al2	UTSW	17	37,983,749 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GAGTGTCAACTGCTCTCTGTG -3'
(R):5'- GGGAGCATATGGCCATGTAG -3'

Sequencing Primer
(F):5'- TGCTCTCTGTGGCAGGAGAAC -3'
(R):5'- AGCATATGGCCATGTAGCGGTC -3'

Posted On

2022-11-14