Mutagenetix > Incidental Mutation

Incidental Mutation 'R9785:Rprm'

734269

Institutional Source

Beutler Lab

Gene Symbol

Rprm

Ensembl Gene

ENSMUSG00000075334

Gene Name

reprimo, TP53 dependent G2 arrest mediator candidate

Synonyms

Reprimo, 2410012A13Rik

MMRRC Submission

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.418) question?

Stock #

R9785 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

53974105-53975564 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 53975238 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Methionine at position 27 (V27M)

Ref Sequence

ENSEMBL: ENSMUSP00000097667 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000100089]

AlphaFold

Q9JJ72

Predicted Effect

probably benign
Transcript: ENSMUST00000100089
AA Change: V27M

PolyPhen 2 Score 0.095 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000097667
Gene: ENSMUSG00000075334
AA Change: V27M

Domain	Start	End	E-Value	Type
transmembrane domain	56	78	N/A	INTRINSIC

Coding Region Coverage

1x: 99.9%
3x: 99.8%
10x: 99.4%
20x: 98.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 40 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamts14	T	C	10: 61,049,427 (GRCm39)	E634G	possibly damaging	Het
Adgre1	T	C	17: 57,785,930 (GRCm39)	L892P	probably damaging	Het
Adgrl1	A	T	8: 84,665,168 (GRCm39)	N1285I	probably damaging	Het
Alpk1	A	T	3: 127,473,594 (GRCm39)	M803K	probably benign	Het
Armt1	T	A	10: 4,389,606 (GRCm39)	W88R	probably damaging	Het
Cacna1d	A	T	14: 29,824,300 (GRCm39)		probably null	Het
Cd27	G	A	6: 125,213,945 (GRCm39)		probably benign	Het
Cebpb	A	G	2: 167,531,663 (GRCm39)	D241G	probably damaging	Het
Clec3b	T	A	9: 122,985,997 (GRCm39)	W132R	probably damaging	Het
Cracd	A	T	5: 77,015,028 (GRCm39)	S1185C	unknown	Het
Csn2	A	T	5: 87,842,502 (GRCm39)	V175D	possibly damaging	Het
Dnm1	A	T	2: 32,223,089 (GRCm39)	C442S	possibly damaging	Het
Dstyk	C	T	1: 132,381,038 (GRCm39)	T511I	probably damaging	Het
Gabra5	C	T	7: 57,140,584 (GRCm39)	V38I	probably benign	Het
Gjd2	A	T	2: 113,841,747 (GRCm39)	Y243*	probably null	Het
Gtf2b	AATCATC	AATC	3: 142,477,178 (GRCm39)		probably benign	Het
Hrnr	G	A	3: 93,238,861 (GRCm39)	R3033H	unknown	Het
Ivd	G	T	2: 118,710,970 (GRCm39)	R393L	probably damaging	Het
Jam2	T	C	16: 84,571,397 (GRCm39)	L8P	unknown	Het
Klhdc7b	G	T	15: 89,272,621 (GRCm39)	D510Y	probably damaging	Het
Lgals8	A	T	13: 12,462,051 (GRCm39)	F260I	probably damaging	Het
Lrrd1	T	C	5: 3,908,708 (GRCm39)	I660T	probably damaging	Het
Mcm5	C	T	8: 75,844,168 (GRCm39)	S313F	probably benign	Het
Npc1l1	C	T	11: 6,180,090 (GRCm39)	A12T	unknown	Het
Or10j7	C	T	1: 173,011,458 (GRCm39)	R181H	probably benign	Het
Or5d47	T	C	2: 87,804,245 (GRCm39)	T255A	possibly damaging	Het
Scg3	G	T	9: 75,589,322 (GRCm39)	D110E	probably damaging	Het
Selenon	C	T	4: 134,270,374 (GRCm39)	G326D	probably damaging	Het
Sh3gl2	T	A	4: 85,273,618 (GRCm39)	Y57N	probably damaging	Het
Skint6	A	T	4: 112,740,884 (GRCm39)	L807M	possibly damaging	Het
Srl	A	G	16: 4,314,718 (GRCm39)	V308A	probably benign	Het
Tcf7	T	A	11: 52,173,773 (GRCm39)		probably benign	Het
Trdv2-2	A	T	14: 54,198,489 (GRCm39)	T14S	probably benign	Het
Usf3	A	G	16: 44,041,970 (GRCm39)	E2150G	probably benign	Het
Vmn2r104	G	A	17: 20,268,409 (GRCm39)	T20I	probably benign	Het
Wnt1	G	T	15: 98,688,752 (GRCm39)	V38L	probably benign	Het
Zbtb17	C	T	4: 141,194,271 (GRCm39)	T740M	possibly damaging	Het
Zdhhc6	T	C	19: 55,300,203 (GRCm39)	M125V	probably damaging	Het
Zfp202	A	G	9: 40,120,233 (GRCm39)	M225V	probably benign	Het
Zfp592	G	T	7: 80,673,245 (GRCm39)	V70F	probably damaging	Het

Other mutations in Rprm

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02963:Rprm	APN	2	53,975,226 (GRCm39)	missense	probably benign	0.19
R0504:Rprm	UTSW	2	53,975,067 (GRCm39)	missense	probably damaging	0.99
R1302:Rprm	UTSW	2	53,975,165 (GRCm39)	missense	probably benign	0.10
R1636:Rprm	UTSW	2	53,975,316 (GRCm39)	start codon destroyed	probably null	0.66
R4763:Rprm	UTSW	2	53,975,228 (GRCm39)	missense	possibly damaging	0.82
R7211:Rprm	UTSW	2	53,975,270 (GRCm39)	missense	probably benign	0.22

Predicted Primers

PCR Primer
(F):5'- GGACTTGATGAGCAGGTTGC -3'
(R):5'- CCTGGGACTTTGAAAGGGAG -3'

Sequencing Primer
(F):5'- GCAGGTTGCAGCCAAGG -3'
(R):5'- CTGCGCGCACAAGTGATCAAG -3'

Posted On

2022-11-14