Mutagenetix > Incidental Mutation

Incidental Mutation 'R9786:Olfr835'

734338

Institutional Source

Beutler Lab

Gene Symbol

Olfr835

Ensembl Gene

ENSMUSG00000045204

Gene Name

olfactory receptor 835

Synonyms

MOR150-3, GA_x6K02T2PVTD-12771995-12772930

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.133) question?

Stock #

R9786 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

19032735-19037670 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 19035945 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Alanine to Valine at position 274 (A274V)

Ref Sequence

ENSEMBL: ENSMUSP00000148416 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000059315] [ENSMUST00000213018]

AlphaFold

Q7TRG7

Predicted Effect

possibly damaging
Transcript: ENSMUST00000059315
AA Change: A274V

PolyPhen 2 Score 0.865 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000052610
Gene: ENSMUSG00000045204
AA Change: A274V

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.8e-47	PFAM
Pfam:7TM_GPCR_Srsx	35	206	2.9e-7	PFAM
Pfam:7tm_1	41	303	1.3e-23	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000213018
AA Change: A274V

PolyPhen 2 Score 0.865 (Sensitivity: 0.83; Specificity: 0.93)

Coding Region Coverage

1x: 99.9%
3x: 99.8%
10x: 99.2%
20x: 97.9%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700014D04Rik	C	T	13: 59,742,684	D441N	possibly damaging	Het
A4gnt	T	A	9: 99,620,483	M232K	possibly damaging	Het
Abcc1	T	C	16: 14,405,063	S243P	probably damaging	Het
Acsl1	T	C	8: 46,521,449	Y320H	probably damaging	Het
Adam11	A	G	11: 102,762,264	S61G	probably benign	Het
Adamts1	G	A	16: 85,795,414	T965I	probably benign	Het
Adcyap1r1	T	A	6: 55,479,197	M190K	probably damaging	Het
Ankrd2	T	A	19: 42,044,919	L300Q		Het
Ankrd27	A	T	7: 35,591,869	Q30L	possibly damaging	Het
Atp8b5	T	A	4: 43,305,798	I114K	probably damaging	Het
Atrn	A	T	2: 130,944,889	I205F	probably damaging	Het
BC035947	A	G	1: 78,511,924		probably benign	Het
Boc	C	T	16: 44,491,329	R677H		Het
D3Ertd254e	T	A	3: 36,165,704	C625*	probably null	Het
Dpysl3	G	T	18: 43,329,857	T485K	probably damaging	Het
Esyt3	T	C	9: 99,311,985	D867G	possibly damaging	Het
Fam178b	G	A	1: 36,564,436	T478I	probably damaging	Het
Fam26f	A	G	10: 34,127,647	F88S	probably benign	Het
Foxd2	G	T	4: 114,907,653	T390K	possibly damaging	Het
Furin	A	G	7: 80,390,897	V731A	probably benign	Het
Gm6583	T	A	5: 112,355,073	E255V	probably benign	Het
Grin2a	T	A	16: 9,653,602	I601F	possibly damaging	Het
Hat1	G	A	2: 71,420,615	R169Q	possibly damaging	Het
Ighe	G	C	12: 113,273,231	Q6E		Het
Ighv8-2	T	C	12: 114,462,559	I31V	probably benign	Het
Klk1	A	G	7: 44,228,680	D120G	probably damaging	Het
Kmt2e	A	G	5: 23,497,984	D1054G	probably benign	Het
Lamp5	A	T	2: 136,069,078	I244F	probably damaging	Het
Mcm5	C	T	8: 75,117,540	S313F	probably benign	Het
Mfsd4b1	T	C	10: 40,002,869	E344G	probably damaging	Het
Mns1	A	G	9: 72,439,274	K13R	probably benign	Het
Mrpl43	C	T	19: 45,005,907	S91N	probably benign	Het
Mtbp	T	C	15: 55,617,636	S706P	probably benign	Het
Nelfb	A	T	2: 25,205,133	V348D	probably damaging	Het
Olfr1049	G	A	2: 86,255,084	S203L	probably benign	Het
Olfr281	T	C	15: 98,456,832	I174T	possibly damaging	Het
Olfr355	T	A	2: 36,927,404	K237*	probably null	Het
Olfr497	A	T	7: 108,422,717	I49F	probably benign	Het
Pde6c	T	C	19: 38,151,561	I324T	possibly damaging	Het
Phlpp2	T	A	8: 109,934,023	L770*	probably null	Het
Pik3c2b	T	A	1: 133,091,600	F1029I	possibly damaging	Het
Rfx2	A	T	17: 56,780,890	S500R	probably benign	Het
Sarm1	T	C	11: 78,474,917	M761V	probably benign	Het
Serpina1a	A	G	12: 103,855,881	L264P	possibly damaging	Het
Slc37a1	G	T	17: 31,337,991	G377V	probably damaging	Het
Slc38a4	T	A	15: 97,008,497	M364L	probably damaging	Het
Slc7a15	A	G	12: 8,530,280	F384S	probably benign	Het
Smyd4	T	C	11: 75,390,799	V366A	probably benign	Het
Spata31d1b	T	A	13: 59,718,341	V1101D	possibly damaging	Het
Stab2	C	A	10: 86,922,133	M1090I	probably benign	Het
Tex15	C	T	8: 33,572,429	T903I	probably damaging	Het
Tgds	A	T	14: 118,130,637	Y41*	probably null	Het
Tle4	G	T	19: 14,517,940	H142N	probably benign	Het
Tlx2	T	C	6: 83,069,293		probably null	Het
Tmem151a	C	G	19: 5,081,841	A446P	probably damaging	Het
Tmem205	A	T	9: 21,921,200	D138E	probably damaging	Het
Tmem57	A	T	4: 134,830,682	Y173*	probably null	Het
Tmprss9	A	G	10: 80,898,208	K1009E	unknown	Het
Tnk2	T	A	16: 32,680,057	C729*	probably null	Het
Trim46	T	A	3: 89,235,092	D696V	probably damaging	Het
Tuba3b	G	A	6: 145,618,756	R84Q	probably benign	Het
Unc119b	C	T	5: 115,125,462	D228N	probably damaging	Het
Usp17la	A	G	7: 104,861,657	T490A	probably benign	Het
Zfp687	C	T	3: 95,012,457	M1I	probably null	Het
Zfp729a	T	C	13: 67,620,509	T534A	possibly damaging	Het
Zfp985	A	T	4: 147,583,590	H305L	probably benign	Het
Zscan10	C	T	17: 23,609,356	Q291*	probably null	Het

Other mutations in Olfr835

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01718:Olfr835	APN	9	19035288	nonsense	probably null
IGL02546:Olfr835	APN	9	19035354	missense	possibly damaging	0.88
R0010:Olfr835	UTSW	9	19035322	missense	probably damaging	1.00
R0107:Olfr835	UTSW	9	19035333	missense	probably damaging	1.00
R1867:Olfr835	UTSW	9	19035266	missense	probably benign	0.36
R1891:Olfr835	UTSW	9	19035978	missense	probably damaging	0.99
R1941:Olfr835	UTSW	9	19035866	missense	possibly damaging	0.93
R4152:Olfr835	UTSW	9	19035520	nonsense	probably null
R5451:Olfr835	UTSW	9	19035491	missense	probably damaging	1.00
R5677:Olfr835	UTSW	9	19035558	missense	possibly damaging	0.93
R5970:Olfr835	UTSW	9	19035147	missense	probably benign
R6187:Olfr835	UTSW	9	19035393	missense	probably benign	0.12
R6805:Olfr835	UTSW	9	19035301	missense	probably damaging	1.00
R7183:Olfr835	UTSW	9	19035332	missense	probably damaging	1.00
R8029:Olfr835	UTSW	9	19035794	missense	probably damaging	1.00
R8139:Olfr835	UTSW	9	19035575	missense	probably benign	0.07
R8736:Olfr835	UTSW	9	19035478	missense	probably damaging	1.00
R8868:Olfr835	UTSW	9	19035482	missense	probably damaging	1.00
R8909:Olfr835	UTSW	9	19035592	missense	probably benign	0.08
R9116:Olfr835	UTSW	9	19035477	missense	probably damaging	1.00
R9479:Olfr835	UTSW	9	19035434	missense	probably benign	0.30

Predicted Primers

PCR Primer
(F):5'- ATATTTGGTGGCATCCCAATCTC -3'
(R):5'- TCATAGGAAACCCTGCTTTCAG -3'

Sequencing Primer
(F):5'- GCATCCCAATCTCTGGTATTATTTTC -3'
(R):5'- AGGAAACCCTGCTTTCAGTATATTCC -3'

Posted On

2022-11-14