Mutagenetix > Incidental Mutation

Incidental Mutation 'R9794:Scart2'

734818

Institutional Source

Beutler Lab

Gene Symbol

Scart2

Ensembl Gene

ENSMUSG00000054672

Gene Name

scavenger receptor family member expressed on T cells 2

Synonyms

5830411N06Rik

MMRRC Submission

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R9794 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

139827197-139880649 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 139874716 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Aspartic acid at position 398 (G398D)

Ref Sequence

ENSEMBL: ENSMUSP00000131905 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000093984] [ENSMUST00000164583]

AlphaFold

B3F5L4

Predicted Effect

probably damaging
Transcript: ENSMUST00000093984
AA Change: G282D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000091520
Gene: ENSMUSG00000054672
AA Change: G282D

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
SR	29	130	1.49e-18	SMART
SR	137	233	2.53e-4	SMART
SR	238	336	1.65e-34	SMART
SR	340	440	4.53e-32	SMART
SR	446	546	8.78e-30	SMART
SR	551	651	1.26e-53	SMART
SR	656	756	2.88e-16	SMART
SR	783	883	7.62e-48	SMART
transmembrane domain	903	925	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000164583
AA Change: G398D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000131905
Gene: ENSMUSG00000054672
AA Change: G398D

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
SR	29	130	1.49e-18	SMART
SR	137	233	2.53e-4	SMART
Blast:SR	291	349	5e-12	BLAST
SR	354	452	1.65e-34	SMART
SR	456	556	4.53e-32	SMART
SR	562	662	8.78e-30	SMART
SR	667	767	1.26e-53	SMART
SR	772	872	2.88e-16	SMART
SR	899	999	7.62e-48	SMART
transmembrane domain	1019	1041	N/A	INTRINSIC

Coding Region Coverage

1x: 99.9%
3x: 99.8%
10x: 99.5%
20x: 99.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9930012K11Rik	T	A	14: 70,395,038 (GRCm39)	I39L	possibly damaging	Het
Acacb	T	C	5: 114,387,578 (GRCm39)	V2415A	probably benign	Het
Adgrg6	C	A	10: 14,314,196 (GRCm39)	G669C	probably damaging	Het
Ahcyl	A	G	16: 45,974,342 (GRCm39)	V345A	probably benign	Het
Arhgdib	A	G	6: 136,906,608 (GRCm39)		probably null	Het
Arhgef18	G	A	8: 3,501,634 (GRCm39)	V643I	probably benign	Het
Aspm	T	A	1: 139,406,480 (GRCm39)	I1789N	probably damaging	Het
Atm	C	T	9: 53,429,867 (GRCm39)	V390I	probably benign	Het
AW554918	A	G	18: 25,337,031 (GRCm39)	E148G	probably damaging	Het
Bmal2	T	A	6: 146,734,033 (GRCm39)	D543E	probably benign	Het
Camk2a	T	C	18: 61,097,031 (GRCm39)	V327A	probably benign	Het
Celsr3	T	C	9: 108,728,502 (GRCm39)	V3302A	probably benign	Het
Ces2a	G	A	8: 105,467,896 (GRCm39)	V509M	probably benign	Het
Cpa5	T	C	6: 30,625,920 (GRCm39)		probably null	Het
Cplx3	A	T	9: 57,509,522 (GRCm39)	*159R	probably null	Het
Cyp2b23	A	T	7: 26,381,121 (GRCm39)	Y79N	probably benign	Het
Dapk1	A	G	13: 60,909,082 (GRCm39)	T1232A	probably damaging	Het
Dsg3	A	T	18: 20,673,154 (GRCm39)	T942S	probably benign	Het
Efnb3	G	A	11: 69,448,232 (GRCm39)	P70L	probably damaging	Het
Eln	G	T	5: 134,751,352 (GRCm39)	Y280*	probably null	Het
Epha8	G	T	4: 136,666,035 (GRCm39)	H374N	probably benign	Het
Erbin	A	T	13: 103,971,359 (GRCm39)	D752E	probably benign	Het
Fam149a	A	G	8: 45,834,449 (GRCm39)	S117P	possibly damaging	Het
Fchsd2	G	A	7: 100,893,410 (GRCm39)	C304Y	probably benign	Het
Galnt6	C	A	15: 100,595,859 (GRCm39)	V390L	probably damaging	Het
Grk3	T	C	5: 113,121,448 (GRCm39)		probably null	Het
Il17rc	A	T	6: 113,453,726 (GRCm39)	T237S	probably benign	Het
Inpp5b	A	G	4: 124,687,174 (GRCm39)	D800G	probably damaging	Het
Itga11	A	G	9: 62,662,868 (GRCm39)	N528S	probably benign	Het
Kif19b	T	A	5: 140,448,070 (GRCm39)		probably null	Het
Lancl2	T	A	6: 57,714,708 (GRCm39)	F442L	probably benign	Het
Ldah	G	T	12: 8,318,430 (GRCm39)	A180S	possibly damaging	Het
Lingo1	T	C	9: 56,528,592 (GRCm39)	E5G	probably benign	Het
Lingo3	T	C	10: 80,670,707 (GRCm39)	I408V	possibly damaging	Het
Liph	A	T	16: 21,774,862 (GRCm39)	L445Q	probably damaging	Het
Lonrf1	A	G	8: 36,703,235 (GRCm39)	Y314H	probably damaging	Het
Lrit3	A	T	3: 129,594,073 (GRCm39)	L168Q	probably damaging	Het
Mideas	T	A	12: 84,220,576 (GRCm39)	Q126L	probably damaging	Het
Mylk4	G	T	13: 32,899,950 (GRCm39)	T312K	probably damaging	Het
Myzap	A	T	9: 71,487,082 (GRCm39)	M15K	probably benign	Het
Ngf	G	A	3: 102,428,132 (GRCm39)	V298M	probably damaging	Het
Nlrp9a	A	G	7: 26,264,302 (GRCm39)	I741V	probably benign	Het
Nrxn2	T	C	19: 6,567,064 (GRCm39)	V1313A	possibly damaging	Het
Nuf2	C	T	1: 169,334,954 (GRCm39)		probably null	Het
Or13a27	G	A	7: 139,925,483 (GRCm39)	Q140*	probably null	Het
Or5m8	A	G	2: 85,822,464 (GRCm39)	Y101C	probably benign	Het
Pcdh17	C	T	14: 84,770,350 (GRCm39)	R943*	probably null	Het
Phykpl	A	T	11: 51,489,212 (GRCm39)	H346L	probably benign	Het
Plk4	C	A	3: 40,759,535 (GRCm39)	L144I	probably damaging	Het
Pole	C	A	5: 110,466,201 (GRCm39)	P1301Q	probably benign	Het
Prkch	T	A	12: 73,744,744 (GRCm39)	N252K	possibly damaging	Het
Psen2	A	T	1: 180,068,294 (GRCm39)		probably null	Het
Ptgds	C	A	2: 25,359,129 (GRCm39)	R42L	probably benign	Het
Ptprz1	A	G	6: 23,000,204 (GRCm39)	T765A	probably benign	Het
Rab6b	T	C	9: 103,041,061 (GRCm39)	F152L	possibly damaging	Het
Rbm12b2	T	A	4: 12,095,335 (GRCm39)	F731L	probably damaging	Het
Resf1	T	A	6: 149,228,239 (GRCm39)	Y428*	probably null	Het
Rnf32	T	C	5: 29,429,125 (GRCm39)	I234T	probably damaging	Het
Scn8a	T	A	15: 100,933,332 (GRCm39)	I1512N	probably benign	Het
Senp6	T	A	9: 79,999,590 (GRCm39)	D81E	probably benign	Het
Slc26a7	T	G	4: 14,590,416 (GRCm39)	N125T	possibly damaging	Het
Sptlc1	A	T	13: 53,512,803 (GRCm39)	I185N	possibly damaging	Het
Syne2	C	A	12: 76,047,617 (GRCm39)	H4013N	probably benign	Het
Tbc1d9b	T	C	11: 50,062,005 (GRCm39)	V1171A	probably benign	Het
Tmem63b	T	C	17: 45,977,252 (GRCm39)	N417S	probably benign	Het
Ttn	A	G	2: 76,706,193 (GRCm39)	V9176A	unknown	Het
Vmn1r210	T	C	13: 23,011,432 (GRCm39)	I285V	probably damaging	Het
Wbp11	C	T	6: 136,795,021 (GRCm39)	V308I	possibly damaging	Het
Wnk2	A	C	13: 49,229,674 (GRCm39)	V950G	probably benign	Het
Zfp110	A	G	7: 12,578,521 (GRCm39)	N144S	probably benign	Het

Other mutations in Scart2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00465:Scart2	APN	7	139,874,755 (GRCm39)	missense	probably damaging	0.99
IGL01101:Scart2	APN	7	139,876,017 (GRCm39)	missense	probably benign	0.35
IGL01120:Scart2	APN	7	139,876,472 (GRCm39)	missense	probably benign	0.02
IGL01958:Scart2	APN	7	139,854,040 (GRCm39)	missense	probably damaging	1.00
IGL02150:Scart2	APN	7	139,877,772 (GRCm39)	missense	possibly damaging	0.84
IGL02193:Scart2	APN	7	139,828,913 (GRCm39)	missense	probably benign	0.17
IGL02239:Scart2	APN	7	139,875,756 (GRCm39)	missense	probably damaging	1.00
IGL02335:Scart2	APN	7	139,876,453 (GRCm39)	missense	probably damaging	1.00
IGL02569:Scart2	APN	7	139,878,275 (GRCm39)	missense	probably benign	0.01
IGL02993:Scart2	APN	7	139,876,486 (GRCm39)	missense	probably benign	0.07
IGL03261:Scart2	APN	7	139,874,746 (GRCm39)	missense	probably benign	0.00
IGL03365:Scart2	APN	7	139,876,682 (GRCm39)	missense	probably damaging	1.00
IGL03399:Scart2	APN	7	139,827,869 (GRCm39)	missense	probably benign	0.00
IGL03052:Scart2	UTSW	7	139,828,827 (GRCm39)	missense	probably damaging	1.00
PIT4791001:Scart2	UTSW	7	139,853,975 (GRCm39)	missense	possibly damaging	0.53
R0021:Scart2	UTSW	7	139,876,310 (GRCm39)	missense	probably benign	0.15
R0021:Scart2	UTSW	7	139,876,310 (GRCm39)	missense	probably benign	0.15
R0347:Scart2	UTSW	7	139,877,767 (GRCm39)	missense	probably damaging	1.00
R0374:Scart2	UTSW	7	139,828,874 (GRCm39)	missense	probably damaging	1.00
R0639:Scart2	UTSW	7	139,827,872 (GRCm39)	missense	probably benign	0.01
R0667:Scart2	UTSW	7	139,841,450 (GRCm39)	missense	possibly damaging	0.73
R0789:Scart2	UTSW	7	139,828,133 (GRCm39)	missense	probably damaging	1.00
R0959:Scart2	UTSW	7	139,874,704 (GRCm39)	missense	probably damaging	1.00
R1316:Scart2	UTSW	7	139,879,583 (GRCm39)	missense	probably benign	0.09
R1764:Scart2	UTSW	7	139,877,178 (GRCm39)	missense	probably benign	0.00
R2247:Scart2	UTSW	7	139,829,042 (GRCm39)	missense	probably null	0.96
R2379:Scart2	UTSW	7	139,879,682 (GRCm39)	missense	probably benign	0.15
R4112:Scart2	UTSW	7	139,878,281 (GRCm39)	nonsense	probably null
R4114:Scart2	UTSW	7	139,877,823 (GRCm39)	missense	probably damaging	1.00
R4346:Scart2	UTSW	7	139,827,878 (GRCm39)	missense	probably damaging	0.97
R4836:Scart2	UTSW	7	139,879,021 (GRCm39)	missense	probably benign
R4956:Scart2	UTSW	7	139,878,275 (GRCm39)	missense	probably benign	0.00
R5208:Scart2	UTSW	7	139,877,949 (GRCm39)	missense	probably benign	0.00
R5571:Scart2	UTSW	7	139,829,036 (GRCm39)	missense	probably damaging	1.00
R5583:Scart2	UTSW	7	139,876,739 (GRCm39)	missense	probably damaging	1.00
R5645:Scart2	UTSW	7	139,828,853 (GRCm39)	missense	possibly damaging	0.95
R6183:Scart2	UTSW	7	139,875,947 (GRCm39)	missense	possibly damaging	0.82
R6995:Scart2	UTSW	7	139,841,514 (GRCm39)	missense	probably benign
R7436:Scart2	UTSW	7	139,841,520 (GRCm39)	missense	probably benign
R7621:Scart2	UTSW	7	139,876,742 (GRCm39)	missense	probably damaging	1.00
R7662:Scart2	UTSW	7	139,874,725 (GRCm39)	missense	possibly damaging	0.58
R7669:Scart2	UTSW	7	139,876,234 (GRCm39)	missense	possibly damaging	0.47
R7686:Scart2	UTSW	7	139,828,965 (GRCm39)	missense	probably benign	0.00
R7985:Scart2	UTSW	7	139,876,806 (GRCm39)	missense	probably damaging	1.00
R8330:Scart2	UTSW	7	139,876,231 (GRCm39)	nonsense	probably null
R8843:Scart2	UTSW	7	139,828,913 (GRCm39)	missense	possibly damaging	0.93
R8888:Scart2	UTSW	7	139,841,532 (GRCm39)	missense	possibly damaging	0.93
R8895:Scart2	UTSW	7	139,841,532 (GRCm39)	missense	possibly damaging	0.93
R9044:Scart2	UTSW	7	139,828,010 (GRCm39)	missense	probably damaging	1.00
R9142:Scart2	UTSW	7	139,877,806 (GRCm39)	missense	probably damaging	1.00
R9152:Scart2	UTSW	7	139,877,256 (GRCm39)	missense	possibly damaging	0.55
R9470:Scart2	UTSW	7	139,827,345 (GRCm39)	missense	probably benign	0.07
R9509:Scart2	UTSW	7	139,879,644 (GRCm39)	nonsense	probably null
R9522:Scart2	UTSW	7	139,853,987 (GRCm39)	missense	possibly damaging	0.73
R9755:Scart2	UTSW	7	139,841,544 (GRCm39)	critical splice donor site	probably null

Predicted Primers

PCR Primer
(F):5'- ATTCCTCACTAGGGGACTGG -3'
(R):5'- TTTCCCCTGAGCAGATTAGTG -3'

Sequencing Primer
(F):5'- GGGTGACAGCCATCAGATCATTTC -3'
(R):5'- AGTGCAGCATCCTGATCATG -3'

Posted On

2022-11-14