Mutagenetix > Incidental Mutation

Incidental Mutation 'R9799:Gstp2'

735132

Institutional Source

Beutler Lab

Gene Symbol

Gstp2

Ensembl Gene

ENSMUSG00000038155

Gene Name

glutathione S-transferase, pi 2

Synonyms

Gst3, Gst p-2, GSTpiA, Gst-3

MMRRC Submission

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.412) question?

Stock #

R9799 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

4090288-4092221 bp(-) (GRCm39)

Type of Mutation

critical splice donor site (2 bp from exon)

DNA Base Change (assembly)

A to G at 4091901 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000038931 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000042700] [ENSMUST00000169613]

AlphaFold

P46425

Predicted Effect

probably null
Transcript: ENSMUST00000042700

SMART Domains

Protein: ENSMUSP00000038931
Gene: ENSMUSG00000038155

Domain	Start	End	E-Value	Type
Pfam:GST_N	2	75	4.2e-8	PFAM
Pfam:GST_C	97	188	1.4e-15	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000169613

SMART Domains

Protein: ENSMUSP00000129565
Gene: ENSMUSG00000060803

Domain	Start	End	E-Value	Type
Pfam:GST_N	4	75	2.3e-8	PFAM
Pfam:GST_C	97	188	1.5e-15	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.8%
10x: 99.5%
20x: 98.7%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mutant mice with null mutations in both Gstp1 and Gstp2 exhibit an increased susceptibility to DMBA and TPA induced skin papillomas. Male mutant mice exhibit an increased body weight with age. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca16	A	G	7: 120,132,998 (GRCm39)	Y1309C	probably benign	Het
Adcy2	T	C	13: 68,768,961 (GRCm39)	T1056A	probably benign	Het
Adcy2	T	A	13: 68,805,489 (GRCm39)	R826S	probably damaging	Het
Agxt	T	C	1: 93,063,070 (GRCm39)	W19R	probably benign	Het
Akr1b8	G	A	6: 34,333,278 (GRCm39)	E52K	possibly damaging	Het
Arhgef10	T	C	8: 14,990,268 (GRCm39)	Y248H	probably damaging	Het
Arhgef38	T	C	3: 132,855,391 (GRCm39)	D232G	unknown	Het
Arid4b	T	C	13: 14,358,967 (GRCm39)	S668P	probably benign	Het
Ccdc136	T	A	6: 29,417,505 (GRCm39)	S775R	probably damaging	Het
Ccl25	T	C	8: 4,377,799 (GRCm39)	F20L	unknown	Het
Cimap1a	A	T	7: 140,430,706 (GRCm39)	E254D	probably benign	Het
Cr2	T	A	1: 194,842,988 (GRCm39)	H218L	probably benign	Het
Csnka2ip	T	C	16: 64,298,672 (GRCm39)	K564R	unknown	Het
Cyp2r1	A	G	7: 114,151,207 (GRCm39)	F371L	probably benign	Het
Ddx50	A	T	10: 62,469,812 (GRCm39)	F392I	probably damaging	Het
Dnah7a	T	C	1: 53,557,968 (GRCm39)	T2149A	probably benign	Het
Emx2	A	G	19: 59,448,036 (GRCm39)	Y130C	possibly damaging	Het
Erbin	T	C	13: 103,971,384 (GRCm39)	D744G	probably benign	Het
Gcm2	T	C	13: 41,258,924 (GRCm39)	N140S	probably damaging	Het
Gnai2	C	T	9: 107,512,380 (GRCm39)	A13T	probably benign	Het
Gpr150	T	C	13: 76,204,636 (GRCm39)	Y103C	possibly damaging	Het
Kdr	A	G	5: 76,117,752 (GRCm39)	I667T	possibly damaging	Het
Lpin1	A	G	12: 16,612,400 (GRCm39)	I534T		Het
Lrrtm3	T	A	10: 63,925,749 (GRCm39)		probably benign	Het
Meiob	C	T	17: 25,042,574 (GRCm39)	T134I	probably benign	Het
Msh2	G	T	17: 88,024,933 (GRCm39)	A604S	probably damaging	Het
Mterf3	T	C	13: 67,062,780 (GRCm39)	T319A	possibly damaging	Het
Myo3a	C	T	2: 22,490,181 (GRCm39)	T1514I	probably damaging	Het
Ndfip2	T	C	14: 105,496,400 (GRCm39)	M98T	possibly damaging	Het
Nfatc2ip	A	G	7: 125,989,739 (GRCm39)	V215A	probably damaging	Het
Or52e19	A	T	7: 102,959,065 (GRCm39)	T46S	probably damaging	Het
Or8k41	A	T	2: 86,313,732 (GRCm39)	M118K	probably damaging	Het
Pabpc6	C	T	17: 9,888,114 (GRCm39)	E146K	probably damaging	Het
Pde3b	A	G	7: 114,122,613 (GRCm39)	Y727C	probably damaging	Het
Pemt	A	G	11: 59,937,174 (GRCm39)	F30L	possibly damaging	Het
Plch1	A	G	3: 63,605,591 (GRCm39)	S1438P	possibly damaging	Het
Pnma2	T	A	14: 67,154,009 (GRCm39)	H144Q	probably benign	Het
Ppp1r14b	A	G	19: 6,952,825 (GRCm39)	N70S	possibly damaging	Het
Psmd1	T	A	1: 86,054,236 (GRCm39)	M725K	possibly damaging	Het
Rab3gap1	A	G	1: 127,858,489 (GRCm39)	K533E	probably benign	Het
Rb1cc1	C	A	1: 6,315,126 (GRCm39)	H426Q	probably damaging	Het
Rimbp3	A	G	16: 17,027,641 (GRCm39)	E355G	possibly damaging	Het
Rxfp1	A	G	3: 79,578,182 (GRCm39)	F170L	probably damaging	Het
Sema3f	G	T	9: 107,562,562 (GRCm39)	A473E	probably damaging	Het
Serpinb3a	T	G	1: 106,974,892 (GRCm39)	I214L	probably benign	Het
Siglec1	G	T	2: 130,915,941 (GRCm39)	P1307Q	probably damaging	Het
Srd5a2	A	C	17: 74,331,535 (GRCm39)	I154S	possibly damaging	Het
Sugp1	A	G	8: 70,523,068 (GRCm39)	E537G	probably damaging	Het
Tbc1d30	T	C	10: 121,142,074 (GRCm39)	E91G	possibly damaging	Het
Tiparp	A	T	3: 65,454,973 (GRCm39)	N373Y	probably benign	Het
Tmem217	A	C	17: 29,745,232 (GRCm39)	I166S	probably damaging	Het
Top1	T	A	2: 160,563,406 (GRCm39)	Y746N	probably damaging	Het
Tubb2a	C	T	13: 34,260,607 (GRCm39)	D31N	probably benign	Het
Utrn	T	G	10: 12,585,736 (GRCm39)	I1014L	probably benign	Het
Vtn	T	C	11: 78,392,625 (GRCm39)	I401T	probably benign	Het
Wwc2	G	T	8: 48,321,595 (GRCm39)	H506Q	unknown	Het
Xab2	G	T	8: 3,668,182 (GRCm39)	T132N	probably benign	Het

Other mutations in Gstp2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02387:Gstp2	APN	19	4,091,094 (GRCm39)	unclassified	probably benign
PIT4362001:Gstp2	UTSW	19	4,090,713 (GRCm39)	missense	possibly damaging	0.82
R0196:Gstp2	UTSW	19	4,090,514 (GRCm39)	splice site	probably null
R0545:Gstp2	UTSW	19	4,091,633 (GRCm39)	missense	possibly damaging	0.88
R6324:Gstp2	UTSW	19	4,090,499 (GRCm39)	missense	probably benign	0.00
R6478:Gstp2	UTSW	19	4,090,499 (GRCm39)	missense	probably benign	0.00
R7318:Gstp2	UTSW	19	4,091,065 (GRCm39)	missense	probably benign	0.00
R8218:Gstp2	UTSW	19	4,091,668 (GRCm39)	missense	probably benign	0.00
Z1177:Gstp2	UTSW	19	4,091,583 (GRCm39)	critical splice donor site	probably null

Predicted Primers

PCR Primer
(F):5'- CCAGCCTCAAAGCAATTTCG -3'
(R):5'- CTGAAGCTTCCCCGAACTAC -3'

Sequencing Primer
(F):5'- TTTCGAGTACAAGGACTGACTGGC -3'
(R):5'- TGGACTGAGCTCTCTGCAC -3'

Posted On

2022-11-14