|Institutional Source||Beutler Lab|
|Gene Name||inositol 1,4,5-trisphosphate 3-kinase B|
|Essential gene?||Probably essential (E-score: 0.789)|
|Stock #||R9652 (G1)|
|Chromosomal Location||180330485-180424802 bp(+) (GRCm38)|
|Type of Mutation||nonsense|
|DNA Base Change (assembly)||G to T at 180332491 bp (GRCm38)|
|Amino Acid Change||Glutamic Acid to Stop codon at position 61 (E61*)|
|Ref Sequence||ENSEMBL: ENSMUSP00000069851 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000070181]|
|PDB Structure||Crystal Structure of the Catalytic and CaM-Binding domains of Inositol 1,4,5-Trisphosphate 3-Kinase B [X-RAY DIFFRACTION]|
AA Change: E61*
AA Change: E61*
|Coding Region Coverage||
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this protein regulates inositol phosphate metabolism by phosphorylation of second messenger inositol 1,4,5-trisphosphate to Ins(1,3,4,5)P4. The activity of this encoded protein is responsible for regulating the levels of a large number of inositol polyphosphates that are important in cellular signaling. Both calcium/calmodulin and protein phosphorylation mechanisms control its activity. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous mutation of this gene results in a block of thymocyte development at the double positive stage. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Itpkb||
(F):5'- ACTGCTATGCCCTCAATAGCC -3'
(R):5'- AGAATCCGCAGCTTCCTCTG -3'
(F):5'- CTCAATAGCCTGGTGATCATGAACAG -3'
(R):5'- GCAGCTTCCTCTGGGCCTC -3'