Mutagenetix > Incidental Mutation

Incidental Mutation 'R9655:Nwd2'

735450

Institutional Source

Beutler Lab

Gene Symbol

Nwd2

Ensembl Gene

ENSMUSG00000090061

Gene Name

NACHT and WD repeat domain containing 2

Synonyms

3110047P20Rik, B830017A01Rik

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.119) question?

Stock #

R9655 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

63806446-63967889 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to A at 63964568 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tryptophan to Stop codon at position 1384 (W1384*)

Ref Sequence

ENSEMBL: ENSMUSP00000124712 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000159584]

AlphaFold

Q6P5U7

Predicted Effect

probably null
Transcript: ENSMUST00000159584
AA Change: W1384*

SMART Domains

Protein: ENSMUSP00000124712
Gene: ENSMUSG00000090061
AA Change: W1384*

Domain	Start	End	E-Value	Type
Pfam:DUF4062	42	145	1.5e-8	PFAM
Blast:AAA	408	691	3e-29	BLAST
WD40	939	995	1.06e2	SMART
WD40	998	1037	8.96e-2	SMART
Blast:WD40	1091	1126	9e-19	BLAST
Blast:WD40	1129	1170	1e-17	BLAST
Blast:WD40	1220	1260	3e-16	BLAST
WD40	1263	1302	3.4e-2	SMART
WD40	1347	1385	2.65e1	SMART
WD40	1386	1425	1.58e2	SMART
Blast:WD40	1466	1507	3e-19	BLAST
Blast:WD40	1606	1644	4e-18	BLAST
Blast:KR	1686	1730	2e-16	BLAST

Predicted Effect

probably benign
Transcript: ENSMUST00000162757

Coding Region Coverage

1x: 99.9%
3x: 99.8%
10x: 99.4%
20x: 98.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 83 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933402N03Rik	T	A	7: 130,740,695 (GRCm39)	I174F	possibly damaging	Het
Ada	C	A	2: 163,574,270 (GRCm39)	V129F	probably damaging	Het
Adgrl4	G	T	3: 151,248,450 (GRCm39)	M707I	probably damaging	Het
Ahcyl	A	T	16: 45,974,564 (GRCm39)	I271N	probably damaging	Het
Arrb2	A	G	11: 70,331,073 (GRCm39)	Q419R	probably null	Het
Atad2	A	T	15: 57,998,303 (GRCm39)	L23Q	probably damaging	Het
Btbd2	A	T	10: 80,492,045 (GRCm39)	F107Y	probably benign	Het
C7	T	A	15: 5,041,464 (GRCm39)	T481S	probably damaging	Het
Capn10	T	A	1: 92,867,111 (GRCm39)	W114R	probably damaging	Het
Ccdc121	C	T	5: 31,644,976 (GRCm39)	T243I	probably benign	Het
Cdc16	T	A	8: 13,809,153 (GRCm39)	D39E	possibly damaging	Het
Cfap74	A	T	4: 155,522,665 (GRCm39)	I684F		Het
Cyp2c70	T	A	19: 40,149,121 (GRCm39)	N342Y	possibly damaging	Het
Dnah17	G	A	11: 117,971,649 (GRCm39)	T2128I	possibly damaging	Het
Dnah5	A	G	15: 28,242,900 (GRCm39)	N545S	probably benign	Het
Dnmt3c	A	G	2: 153,561,914 (GRCm39)	N539S	probably damaging	Het
Elfn1	A	G	5: 139,958,964 (GRCm39)	E656G	possibly damaging	Het
Exd1	A	T	2: 119,350,855 (GRCm39)	C469S	probably damaging	Het
F5	A	T	1: 164,021,730 (GRCm39)	I1402F	probably benign	Het
Fam186a	A	G	15: 99,840,973 (GRCm39)	L1757P	probably damaging	Het
Fbxo42	A	T	4: 140,895,171 (GRCm39)	R45W	probably damaging	Het
Fer1l5	T	C	1: 36,460,696 (GRCm39)	V1978A	probably benign	Het
Fpr1	T	A	17: 18,097,618 (GRCm39)	I124F	probably damaging	Het
Fry	A	T	5: 150,362,251 (GRCm39)	D2173V	possibly damaging	Het
Gabbr2	G	T	4: 46,815,684 (GRCm39)	T228K	possibly damaging	Het
Galnt9	A	T	5: 110,762,104 (GRCm39)	Y414F	probably damaging	Het
Gask1a	T	A	9: 121,794,170 (GRCm39)	L108Q	probably benign	Het
Ggt5	A	T	10: 75,444,635 (GRCm39)	M318L	probably benign	Het
Glod4	A	C	11: 76,125,292 (GRCm39)	S156A	probably benign	Het
Gm5431	A	T	11: 48,785,799 (GRCm39)	M192K	probably benign	Het
Golga5	C	T	12: 102,446,008 (GRCm39)	S421L	possibly damaging	Het
Gpr18	T	C	14: 122,149,992 (GRCm39)	D11G	probably benign	Het
Igf2bp3	C	T	6: 49,064,338 (GRCm39)	V560I	probably benign	Het
Igfals	A	G	17: 25,099,665 (GRCm39)	N252S	probably damaging	Het
Ighv8-5	C	T	12: 115,031,416 (GRCm39)	C41Y	probably damaging	Het
Iqgap3	T	A	3: 88,016,728 (GRCm39)	V1070D	possibly damaging	Het
Kdr	G	A	5: 76,122,488 (GRCm39)	A479V	probably benign	Het
Kidins220	T	C	12: 25,047,295 (GRCm39)	L247P	probably damaging	Het
Kmt5a	A	G	5: 124,589,393 (GRCm39)	Y197C	probably damaging	Het
Krt33a	A	C	11: 99,906,624 (GRCm39)		probably null	Het
Mageb3	A	G	2: 121,785,649 (GRCm39)	S18P	unknown	Het
Mast1	T	A	8: 85,650,660 (GRCm39)	Y387F	probably damaging	Het
Mcm5	C	T	8: 75,844,168 (GRCm39)	S313F	probably benign	Het
Med17	C	T	9: 15,176,719 (GRCm39)	V503M	possibly damaging	Het
Mpp3	A	G	11: 101,899,481 (GRCm39)	C347R	probably benign	Het
Mtr	A	G	13: 12,203,030 (GRCm39)	L1191P	probably damaging	Het
Mybphl	A	T	3: 108,282,099 (GRCm39)	I110F	probably damaging	Het
Nkx2-1	T	A	12: 56,581,802 (GRCm39)	D15V	probably damaging	Het
Onecut1	A	G	9: 74,770,330 (GRCm39)	H251R	possibly damaging	Het
Or10ak8	T	A	4: 118,773,804 (GRCm39)	N287Y	probably damaging	Het
Or13f5	C	T	4: 52,825,526 (GRCm39)	T43I	probably benign	Het
Or51a6	A	G	7: 102,604,319 (GRCm39)	F163S	probably damaging	Het
Or8b37	A	T	9: 37,959,387 (GRCm39)	I290L	probably benign	Het
Palmd	T	C	3: 116,716,840 (GRCm39)	*552W	probably null	Het
Pcdhgb2	A	G	18: 37,823,285 (GRCm39)	E92G	probably damaging	Het
Pcdhgb5	G	T	18: 37,865,122 (GRCm39)	E306*	probably null	Het
Peg10	CCACATCAGGATCCACATCAGGATGCACATCAGCATCAGGATCCCCATCAGGATGCACATCAGGATCCACATCAGGATGCACATCAG	CCACATCAGGATCCACATCAGGATGCACATCAG	6: 4,756,398 (GRCm39)		probably benign	Het
Pex1	T	A	5: 3,655,653 (GRCm39)	L160Q	probably damaging	Het
Phxr2	A	T	10: 98,961,974 (GRCm39)	S29T	unknown	Het
Ppargc1a	A	G	5: 51,705,852 (GRCm39)		probably null	Het
Psmb11	T	C	14: 54,862,965 (GRCm39)	V61A	probably damaging	Het
Ripor2	A	T	13: 24,908,983 (GRCm39)	I1034F	possibly damaging	Het
Rnaset2b	T	A	17: 7,259,134 (GRCm39)	N133K	probably damaging	Het
Rtn4	A	G	11: 29,657,504 (GRCm39)	T553A	probably damaging	Het
Samd9l	T	A	6: 3,373,578 (GRCm39)	T1228S	probably benign	Het
Sdr42e2	A	G	7: 120,430,279 (GRCm39)	T379A	probably benign	Het
Shisal2a	C	A	4: 108,234,616 (GRCm39)	V84L	possibly damaging	Het
Slc1a1	C	T	19: 28,870,283 (GRCm39)	A94V	probably damaging	Het
Snx31	C	T	15: 36,534,582 (GRCm39)	C197Y	probably damaging	Het
Tbc1d4	A	G	14: 101,744,567 (GRCm39)	V353A	probably damaging	Het
Tbc1d9b	A	G	11: 50,059,610 (GRCm39)	D1004G	possibly damaging	Het
Tex15	T	A	8: 34,066,784 (GRCm39)	Y2071*	probably null	Het
Tnni3k	G	A	3: 154,645,410 (GRCm39)	R492*	probably null	Het
Tns2	A	G	15: 102,012,933 (GRCm39)	H7R	probably benign	Het
Top2a	G	T	11: 98,905,334 (GRCm39)	N369K	probably damaging	Het
Tpbg	A	T	9: 85,726,252 (GRCm39)	T74S	probably damaging	Het
Trpm6	A	G	19: 18,869,466 (GRCm39)	N2018D	probably benign	Het
Trub2	A	T	2: 29,669,833 (GRCm39)		probably null	Het
Vps13d	A	G	4: 144,813,305 (GRCm39)	F3324L		Het
Wdr20rt	A	T	12: 65,273,707 (GRCm39)	Q390L	probably benign	Het
Wdr49	T	A	3: 75,240,561 (GRCm39)	D436V	probably damaging	Het
Zfp111	C	T	7: 23,898,543 (GRCm39)	G357D	probably damaging	Het
Zfp668	A	G	7: 127,466,113 (GRCm39)	V357A	possibly damaging	Het

Other mutations in Nwd2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00325:Nwd2	APN	5	63,962,818 (GRCm39)	missense	probably benign
IGL01111:Nwd2	APN	5	63,964,643 (GRCm39)	missense	probably damaging	1.00
IGL01152:Nwd2	APN	5	63,963,872 (GRCm39)	missense	possibly damaging	0.74
IGL01307:Nwd2	APN	5	63,965,626 (GRCm39)	missense	possibly damaging	0.95
IGL01449:Nwd2	APN	5	63,962,937 (GRCm39)	missense	probably damaging	1.00
IGL01624:Nwd2	APN	5	63,964,153 (GRCm39)	missense	probably damaging	1.00
IGL01997:Nwd2	APN	5	63,961,938 (GRCm39)	missense	probably damaging	0.99
IGL02007:Nwd2	APN	5	63,962,042 (GRCm39)	missense	possibly damaging	0.87
IGL02143:Nwd2	APN	5	63,948,996 (GRCm39)	splice site	probably null
IGL02184:Nwd2	APN	5	63,963,020 (GRCm39)	missense	probably damaging	1.00
IGL02379:Nwd2	APN	5	63,962,644 (GRCm39)	missense	probably damaging	1.00
IGL02489:Nwd2	APN	5	63,962,570 (GRCm39)	missense	probably damaging	1.00
IGL02580:Nwd2	APN	5	63,965,512 (GRCm39)	missense	probably damaging	0.99
IGL02682:Nwd2	APN	5	63,962,021 (GRCm39)	missense	probably damaging	1.00
IGL02682:Nwd2	APN	5	63,962,020 (GRCm39)	missense	probably benign	0.03
IGL02891:Nwd2	APN	5	63,882,570 (GRCm39)	missense	possibly damaging	0.91
IGL03135:Nwd2	APN	5	63,963,338 (GRCm39)	missense	probably damaging	1.00
IGL03149:Nwd2	APN	5	63,963,338 (GRCm39)	missense	probably damaging	1.00
R0113:Nwd2	UTSW	5	63,965,241 (GRCm39)	missense	probably damaging	1.00
R0172:Nwd2	UTSW	5	63,963,712 (GRCm39)	missense	probably benign	0.44
R0196:Nwd2	UTSW	5	63,963,694 (GRCm39)	missense	probably benign	0.37
R0239:Nwd2	UTSW	5	63,957,467 (GRCm39)	missense	probably benign	0.01
R0239:Nwd2	UTSW	5	63,957,467 (GRCm39)	missense	probably benign	0.01
R0309:Nwd2	UTSW	5	63,964,561 (GRCm39)	missense	probably damaging	1.00
R0311:Nwd2	UTSW	5	63,962,341 (GRCm39)	missense	probably damaging	0.99
R0335:Nwd2	UTSW	5	63,962,116 (GRCm39)	missense	probably benign	0.00
R0384:Nwd2	UTSW	5	63,963,025 (GRCm39)	missense	probably benign	0.11
R0496:Nwd2	UTSW	5	63,963,686 (GRCm39)	missense	probably damaging	0.99
R0497:Nwd2	UTSW	5	63,963,686 (GRCm39)	missense	probably damaging	0.99
R0498:Nwd2	UTSW	5	63,963,686 (GRCm39)	missense	probably damaging	0.99
R0505:Nwd2	UTSW	5	63,962,454 (GRCm39)	missense	probably damaging	1.00
R0655:Nwd2	UTSW	5	63,948,928 (GRCm39)	missense	possibly damaging	0.73
R0762:Nwd2	UTSW	5	63,957,757 (GRCm39)	missense	probably benign	0.33
R0835:Nwd2	UTSW	5	63,957,473 (GRCm39)	missense	probably damaging	0.99
R0926:Nwd2	UTSW	5	63,965,234 (GRCm39)	missense	probably damaging	0.99
R0948:Nwd2	UTSW	5	63,964,655 (GRCm39)	missense	probably damaging	1.00
R1015:Nwd2	UTSW	5	63,964,154 (GRCm39)	missense	probably damaging	1.00
R1086:Nwd2	UTSW	5	63,963,917 (GRCm39)	missense	probably damaging	1.00
R1186:Nwd2	UTSW	5	63,807,367 (GRCm39)	utr 5 prime	probably benign
R1305:Nwd2	UTSW	5	63,902,540 (GRCm39)	missense	probably damaging	0.97
R1542:Nwd2	UTSW	5	63,964,318 (GRCm39)	missense	probably damaging	1.00
R1548:Nwd2	UTSW	5	63,957,525 (GRCm39)	missense	probably benign	0.00
R1553:Nwd2	UTSW	5	63,957,848 (GRCm39)	missense	probably benign	0.00
R1636:Nwd2	UTSW	5	63,964,900 (GRCm39)	missense	probably damaging	1.00
R1658:Nwd2	UTSW	5	63,964,589 (GRCm39)	missense	probably damaging	1.00
R1763:Nwd2	UTSW	5	63,965,614 (GRCm39)	missense	probably benign
R1800:Nwd2	UTSW	5	63,962,917 (GRCm39)	missense	probably benign	0.15
R1813:Nwd2	UTSW	5	63,962,753 (GRCm39)	missense	probably benign	0.00
R1861:Nwd2	UTSW	5	63,962,197 (GRCm39)	missense	probably damaging	0.96
R1889:Nwd2	UTSW	5	63,965,009 (GRCm39)	missense	possibly damaging	0.49
R1896:Nwd2	UTSW	5	63,962,753 (GRCm39)	missense	probably benign	0.00
R1919:Nwd2	UTSW	5	63,963,523 (GRCm39)	missense	probably damaging	1.00
R1922:Nwd2	UTSW	5	63,951,585 (GRCm39)	missense	probably benign
R2258:Nwd2	UTSW	5	63,962,499 (GRCm39)	missense	probably benign	0.00
R2292:Nwd2	UTSW	5	63,962,917 (GRCm39)	missense	probably benign	0.15
R2504:Nwd2	UTSW	5	63,961,717 (GRCm39)	missense	probably benign	0.02
R2869:Nwd2	UTSW	5	63,957,671 (GRCm39)	missense	probably benign	0.00
R2869:Nwd2	UTSW	5	63,957,671 (GRCm39)	missense	probably benign	0.00
R2958:Nwd2	UTSW	5	63,963,325 (GRCm39)	missense	probably benign	0.01
R3034:Nwd2	UTSW	5	63,957,446 (GRCm39)	missense	probably damaging	1.00
R3422:Nwd2	UTSW	5	63,882,536 (GRCm39)	missense	possibly damaging	0.46
R3423:Nwd2	UTSW	5	63,957,504 (GRCm39)	missense	probably damaging	1.00
R3439:Nwd2	UTSW	5	63,961,895 (GRCm39)	missense	probably benign	0.00
R4193:Nwd2	UTSW	5	63,964,808 (GRCm39)	missense	probably damaging	1.00
R4254:Nwd2	UTSW	5	63,963,889 (GRCm39)	missense	possibly damaging	0.74
R4384:Nwd2	UTSW	5	63,963,914 (GRCm39)	missense	probably damaging	1.00
R4707:Nwd2	UTSW	5	63,951,665 (GRCm39)	missense	probably damaging	1.00
R4713:Nwd2	UTSW	5	63,961,803 (GRCm39)	missense	probably benign	0.00
R4735:Nwd2	UTSW	5	63,965,594 (GRCm39)	missense	probably benign	0.34
R4744:Nwd2	UTSW	5	63,964,310 (GRCm39)	missense	probably damaging	1.00
R4795:Nwd2	UTSW	5	63,962,776 (GRCm39)	missense	probably benign	0.21
R4835:Nwd2	UTSW	5	63,965,189 (GRCm39)	missense	probably benign	0.00
R4839:Nwd2	UTSW	5	63,962,893 (GRCm39)	missense	possibly damaging	0.92
R4896:Nwd2	UTSW	5	63,962,151 (GRCm39)	missense	probably damaging	1.00
R5017:Nwd2	UTSW	5	63,807,484 (GRCm39)	utr 5 prime	probably benign
R5170:Nwd2	UTSW	5	63,963,380 (GRCm39)	missense	probably damaging	0.99
R5312:Nwd2	UTSW	5	63,963,415 (GRCm39)	nonsense	probably null
R5330:Nwd2	UTSW	5	63,963,859 (GRCm39)	missense	probably benign	0.02
R5331:Nwd2	UTSW	5	63,963,859 (GRCm39)	missense	probably benign	0.02
R5419:Nwd2	UTSW	5	63,965,051 (GRCm39)	missense	probably benign	0.11
R5434:Nwd2	UTSW	5	63,964,991 (GRCm39)	missense	probably benign	0.00
R5445:Nwd2	UTSW	5	63,962,681 (GRCm39)	missense	probably damaging	1.00
R5761:Nwd2	UTSW	5	63,882,573 (GRCm39)	missense	probably damaging	1.00
R5788:Nwd2	UTSW	5	63,965,114 (GRCm39)	missense	probably benign	0.00
R5907:Nwd2	UTSW	5	63,963,326 (GRCm39)	missense	probably damaging	0.99
R5959:Nwd2	UTSW	5	63,965,413 (GRCm39)	missense	probably benign	0.32
R6002:Nwd2	UTSW	5	63,962,143 (GRCm39)	missense	probably benign
R6027:Nwd2	UTSW	5	63,965,563 (GRCm39)	missense	possibly damaging	0.65
R6082:Nwd2	UTSW	5	63,962,374 (GRCm39)	missense	possibly damaging	0.96
R6163:Nwd2	UTSW	5	63,963,131 (GRCm39)	missense	probably benign	0.00
R6172:Nwd2	UTSW	5	63,964,249 (GRCm39)	missense	probably damaging	0.98
R6334:Nwd2	UTSW	5	63,957,596 (GRCm39)	missense	possibly damaging	0.95
R6447:Nwd2	UTSW	5	63,964,898 (GRCm39)	missense	probably benign	0.41
R6649:Nwd2	UTSW	5	63,882,527 (GRCm39)	missense	possibly damaging	0.89
R6855:Nwd2	UTSW	5	63,961,794 (GRCm39)	missense	probably benign	0.00
R7034:Nwd2	UTSW	5	63,962,258 (GRCm39)	missense	probably damaging	1.00
R7168:Nwd2	UTSW	5	63,964,837 (GRCm39)	missense	probably benign	0.04
R7326:Nwd2	UTSW	5	63,957,752 (GRCm39)	missense	probably damaging	1.00
R7561:Nwd2	UTSW	5	63,964,434 (GRCm39)	nonsense	probably null
R7576:Nwd2	UTSW	5	63,964,736 (GRCm39)	missense	probably benign	0.00
R7580:Nwd2	UTSW	5	63,965,624 (GRCm39)	missense	probably benign	0.05
R7723:Nwd2	UTSW	5	63,965,347 (GRCm39)	missense	possibly damaging	0.69
R7769:Nwd2	UTSW	5	63,961,847 (GRCm39)	missense	probably damaging	0.99
R8293:Nwd2	UTSW	5	63,962,663 (GRCm39)	missense	probably benign	0.05
R8517:Nwd2	UTSW	5	63,948,925 (GRCm39)	missense	probably damaging	1.00
R8782:Nwd2	UTSW	5	63,882,540 (GRCm39)	missense	probably damaging	1.00
R8792:Nwd2	UTSW	5	63,963,047 (GRCm39)	missense	probably damaging	0.97
R8888:Nwd2	UTSW	5	63,963,241 (GRCm39)	missense	probably damaging	1.00
R8895:Nwd2	UTSW	5	63,963,241 (GRCm39)	missense	probably damaging	1.00
R8901:Nwd2	UTSW	5	63,963,685 (GRCm39)	missense	probably damaging	1.00
R8913:Nwd2	UTSW	5	63,963,440 (GRCm39)	missense	possibly damaging	0.80
R8920:Nwd2	UTSW	5	63,948,863 (GRCm39)	missense	probably damaging	1.00
R9052:Nwd2	UTSW	5	63,961,773 (GRCm39)	missense	probably damaging	1.00
R9362:Nwd2	UTSW	5	63,961,747 (GRCm39)	missense	probably benign	0.23
R9368:Nwd2	UTSW	5	63,962,306 (GRCm39)	missense	probably damaging	0.99
R9377:Nwd2	UTSW	5	63,957,740 (GRCm39)	missense	probably damaging	1.00
R9430:Nwd2	UTSW	5	63,964,665 (GRCm39)	missense	probably damaging	1.00
R9661:Nwd2	UTSW	5	63,957,780 (GRCm39)	missense	probably damaging	0.97
R9736:Nwd2	UTSW	5	63,951,600 (GRCm39)	missense	probably damaging	1.00
R9793:Nwd2	UTSW	5	63,964,232 (GRCm39)	missense	probably damaging	1.00
R9795:Nwd2	UTSW	5	63,964,232 (GRCm39)	missense	probably damaging	1.00
RF020:Nwd2	UTSW	5	63,963,066 (GRCm39)	nonsense	probably null
X0023:Nwd2	UTSW	5	63,964,306 (GRCm39)	missense	probably damaging	0.99
Z1176:Nwd2	UTSW	5	63,963,500 (GRCm39)	missense	probably damaging	1.00
Z1176:Nwd2	UTSW	5	63,882,540 (GRCm39)	missense	probably damaging	1.00
Z1177:Nwd2	UTSW	5	63,964,669 (GRCm39)	nonsense	probably null
Z1177:Nwd2	UTSW	5	63,962,327 (GRCm39)	missense	possibly damaging	0.60

Predicted Primers

PCR Primer
(F):5'- ACTGGAAAACCCATTCAGAGTC -3'
(R):5'- TGTTTGCAGAGGTAATGAAGGC -3'

Sequencing Primer
(F):5'- AACCCATTCAGAGTCTGGTG -3'
(R):5'- GCATTTTGCAAAGTGGTCAAG -3'

Posted On

2022-11-14