Incidental Mutation 'R9694:Zfpm2'
ID 735983
Institutional Source Beutler Lab
Gene Symbol Zfpm2
Ensembl Gene ENSMUSG00000022306
Gene Name zinc finger protein, multitype 2
Synonyms B330005D23Rik, FOG2, FOG-2
MMRRC Submission
Accession Numbers
Essential gene? Essential (E-score: 1.000) question?
Stock # R9694 (G1)
Quality Score 225.009
Status Not validated
Chromosome 15
Chromosomal Location 40655035-41104592 bp(+) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) A to G at 41102314 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Threonine to Alanine at position 732 (T732A)
Ref Sequence ENSEMBL: ENSMUSP00000051335 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000053467] [ENSMUST00000230319]
AlphaFold Q8CCH7
Predicted Effect possibly damaging
Transcript: ENSMUST00000053467
AA Change: T732A

PolyPhen 2 Score 0.882 (Sensitivity: 0.82; Specificity: 0.94)
SMART Domains Protein: ENSMUSP00000051335
Gene: ENSMUSG00000022306
AA Change: T732A

DomainStartEndE-ValueType
low complexity region 19 35 N/A INTRINSIC
ZnF_C2H2 250 270 4.27e1 SMART
ZnF_C2H2 296 320 1.25e-1 SMART
ZnF_C2H2 335 357 4.05e-1 SMART
ZnF_C2H2 363 385 6.23e-2 SMART
ZnF_C2H2 548 569 1.43e1 SMART
ZnF_C2H2 687 714 1.06e2 SMART
low complexity region 731 741 N/A INTRINSIC
ZnF_C2H2 854 874 5.4e1 SMART
ZnF_C2H2 1119 1145 4.99e1 SMART
Predicted Effect possibly damaging
Transcript: ENSMUST00000230319
AA Change: T600A

PolyPhen 2 Score 0.882 (Sensitivity: 0.82; Specificity: 0.94)
Coding Region Coverage
  • 1x: 99.8%
  • 3x: 99.7%
  • 10x: 99.3%
  • 20x: 98.7%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The zinc finger protein encoded by this gene is a widely expressed member of the FOG family of transcription factors. The family members modulate the activity of GATA family proteins, which are important regulators of hematopoiesis and cardiogenesis in mammals. It has been demonstrated that the protein can both activate and down-regulate expression of GATA-target genes, suggesting different modulation in different promoter contexts. A related mRNA suggests an alternatively spliced product but this information is not yet fully supported by the sequence. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for targeted null mutations exhibit cardiac defects, including absence of coronary vasculature, resulting in lethality between E12.5 and E15.5. Conditional mutations reveal errors in ovary and testis development. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 77 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abcb1b T A 5: 8,849,573 Y948N probably damaging Het
Adamts2 A G 11: 50,668,145 D229G probably benign Het
Adamts4 T C 1: 171,253,961 S395P probably benign Het
Adcy1 T C 11: 7,144,774 Y567H probably damaging Het
Agap3 A G 5: 24,477,141 N212D probably benign Het
Atp2a2 A G 5: 122,459,645 F775S probably damaging Het
Baz1b A T 5: 135,244,240 Q1406L probably benign Het
C030006K11Rik A T 15: 76,723,728 V30D possibly damaging Het
Ccdc144b A G 3: 36,018,943 S397P possibly damaging Het
Cep170b T A 12: 112,735,559 V262E probably damaging Het
Cgnl1 C T 9: 71,725,521 G183R probably benign Het
Chd8 A G 14: 52,203,884 I2257T possibly damaging Het
Chrna7 A G 7: 63,105,061 I266T probably damaging Het
Cyp3a25 A T 5: 145,986,875 Y319* probably null Het
Dcdc2a A T 13: 25,102,357 I125F probably benign Het
Dcdc2c A T 12: 28,535,554 I80N Het
Dmtn T C 14: 70,615,292 probably null Het
Dock2 T C 11: 34,268,054 N1172S probably benign Het
Dock9 A G 14: 121,581,379 S1564P probably damaging Het
Dusp27 A T 1: 166,101,085 S319R probably damaging Het
Ecel1 T C 1: 87,153,131 I350V possibly damaging Het
Eif4enif1 C T 11: 3,220,384 L146F probably damaging Het
Ergic1 T G 17: 26,624,611 D59E probably benign Het
F2rl2 A T 13: 95,701,542 D365V possibly damaging Het
Fbxl16 C T 17: 25,817,839 Q265* probably null Het
Fbxw26 T C 9: 109,746,067 probably benign Het
Fras1 A T 5: 96,781,686 Y3983F probably benign Het
G6pd2 T A 5: 61,809,117 D78E probably benign Het
Gas2l2 A C 11: 83,423,344 S381A possibly damaging Het
Grk2 G A 19: 4,288,483 R474C probably damaging Het
Gxylt2 T A 6: 100,733,213 V105E probably benign Het
Hexdc T C 11: 121,216,987 V181A probably damaging Het
Ik T A 18: 36,744,787 D5E probably benign Het
Ilkap A G 1: 91,376,251 C163R Het
Itpr3 C T 17: 27,115,953 T2147I probably damaging Het
Kif1a A T 1: 93,022,451 V1418E probably benign Het
Kif26b T C 1: 178,916,250 C1304R probably benign Het
Lmna CAGCACGGTGCGTGAGC CAGC 3: 88,482,550 probably null Het
Lvrn A T 18: 46,900,542 D940V probably damaging Het
Mill1 G A 7: 18,263,102 R206H probably benign Het
Mrgpra1 A T 7: 47,335,520 L137Q probably damaging Het
Npc2 C A 12: 84,760,864 L73F probably benign Het
Olfr1042 T C 2: 86,160,337 E11G probably benign Het
Olfr1052 T C 2: 86,298,374 I186T probably benign Het
Olfr1447 A T 19: 12,901,657 V41E probably damaging Het
Olfr556 A T 7: 102,670,804 I295F possibly damaging Het
Olfr742 A G 14: 50,515,212 T3A probably benign Het
Osbpl1a T C 18: 12,819,508 H533R probably benign Het
Pax7 G A 4: 139,829,508 T147M probably benign Het
Pdik1l A T 4: 134,279,400 S143T unknown Het
Prkg1 T G 19: 30,786,971 T255P possibly damaging Het
Prmt2 A T 10: 76,225,379 I91N probably damaging Het
Ptprm A G 17: 66,809,489 Y932H probably damaging Het
Ptprz1 A T 6: 22,959,695 I64F probably damaging Het
Qrich2 T C 11: 116,447,120 K154R probably damaging Het
Rab8b T C 9: 66,919,542 D31G probably benign Het
Radil A G 5: 142,487,623 S768P probably damaging Het
Rbm5 A T 9: 107,744,953 D607E probably benign Het
Reep6 T A 10: 80,333,559 V131E probably damaging Het
Rrbp1 T A 2: 143,990,179 I23F probably damaging Het
Rubcn A T 16: 32,843,111 V385D probably benign Het
Sall2 C T 14: 52,314,667 G357D possibly damaging Het
Slc12a6 T A 2: 112,344,536 L547H probably damaging Het
Slc16a6 T C 11: 109,463,496 T100A probably benign Het
Sptbn2 T C 19: 4,750,507 L2250P probably damaging Het
Stab1 C T 14: 31,154,944 A808T probably benign Het
Stag1 A G 9: 100,928,098 T785A probably benign Het
Stambpl1 T G 19: 34,234,135 S199A probably benign Het
Supt20 C T 3: 54,715,594 S496L probably benign Het
Tanc1 T C 2: 59,795,852 F518L probably damaging Het
Tdpoz3 T A 3: 93,826,849 V277D probably benign Het
Ttc30a2 T C 2: 75,977,347 T274A probably benign Het
Unc45a A C 7: 80,325,655 Y934D probably damaging Het
Vmn1r192 G A 13: 22,187,949 P34S probably benign Het
Wipf3 T C 6: 54,489,019 S421P possibly damaging Het
Zfp296 A G 7: 19,580,302 K361E possibly damaging Het
Zfp882 T A 8: 71,914,071 N247K probably benign Het
Other mutations in Zfpm2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00473:Zfpm2 APN 15 41099287 missense probably damaging 1.00
IGL00815:Zfpm2 APN 15 41099491 missense probably benign 0.37
IGL00821:Zfpm2 APN 15 41103387 missense probably damaging 1.00
IGL01622:Zfpm2 APN 15 41101924 missense probably benign 0.07
IGL01623:Zfpm2 APN 15 41101924 missense probably benign 0.07
IGL01807:Zfpm2 APN 15 40753056 critical splice donor site probably null
IGL01872:Zfpm2 APN 15 41102387 missense probably benign
IGL02087:Zfpm2 APN 15 41103121 missense probably damaging 0.97
IGL02123:Zfpm2 APN 15 41102195 missense probably damaging 1.00
IGL02355:Zfpm2 APN 15 41099494 missense probably damaging 1.00
IGL02362:Zfpm2 APN 15 41099494 missense probably damaging 1.00
IGL02579:Zfpm2 APN 15 41099472 missense possibly damaging 0.91
IGL02752:Zfpm2 APN 15 41102019 missense probably benign 0.23
IGL02792:Zfpm2 APN 15 41103013 missense probably benign 0.00
IGL02861:Zfpm2 APN 15 41103266 missense probably damaging 0.98
IGL03180:Zfpm2 APN 15 41101394 missense probably damaging 1.00
IGL03344:Zfpm2 APN 15 41102774 missense probably benign
R0305:Zfpm2 UTSW 15 40774035 splice site probably benign
R0365:Zfpm2 UTSW 15 40774066 missense possibly damaging 0.88
R1171:Zfpm2 UTSW 15 41101679 missense probably damaging 1.00
R1456:Zfpm2 UTSW 15 41102481 missense probably damaging 1.00
R1482:Zfpm2 UTSW 15 41099291 missense probably damaging 1.00
R1580:Zfpm2 UTSW 15 41103209 missense possibly damaging 0.84
R2119:Zfpm2 UTSW 15 41103023 missense probably damaging 1.00
R2189:Zfpm2 UTSW 15 41101183 missense possibly damaging 0.76
R2867:Zfpm2 UTSW 15 41099389 missense probably benign 0.06
R2867:Zfpm2 UTSW 15 41099389 missense probably benign 0.06
R2886:Zfpm2 UTSW 15 41102323 missense probably benign 0.44
R3024:Zfpm2 UTSW 15 41102959 missense probably benign 0.00
R4043:Zfpm2 UTSW 15 40870627 missense possibly damaging 0.94
R4178:Zfpm2 UTSW 15 41103544 missense probably damaging 1.00
R4465:Zfpm2 UTSW 15 41096161 missense probably benign 0.00
R5263:Zfpm2 UTSW 15 41099395 missense probably benign 0.45
R5266:Zfpm2 UTSW 15 41099469 missense probably benign 0.01
R5352:Zfpm2 UTSW 15 40870542 missense probably benign 0.01
R5584:Zfpm2 UTSW 15 41102537 missense probably benign 0.45
R5661:Zfpm2 UTSW 15 41096071 nonsense probably null
R6437:Zfpm2 UTSW 15 41099397 missense probably benign
R6660:Zfpm2 UTSW 15 40655585 critical splice donor site probably null
R6742:Zfpm2 UTSW 15 41101718 missense probably benign
R6749:Zfpm2 UTSW 15 40954708 missense possibly damaging 0.90
R7363:Zfpm2 UTSW 15 40753017 missense probably damaging 1.00
R7401:Zfpm2 UTSW 15 41102990 missense possibly damaging 0.87
R7657:Zfpm2 UTSW 15 41103275 missense possibly damaging 0.78
R7690:Zfpm2 UTSW 15 40954766 missense possibly damaging 0.45
R7698:Zfpm2 UTSW 15 41096091 missense probably benign 0.03
R7893:Zfpm2 UTSW 15 41102612 missense probably damaging 1.00
R8081:Zfpm2 UTSW 15 41102248 missense probably damaging 1.00
R8223:Zfpm2 UTSW 15 40752959 missense probably benign 0.34
R9028:Zfpm2 UTSW 15 41103362 missense possibly damaging 0.87
R9065:Zfpm2 UTSW 15 41099316 missense possibly damaging 0.95
R9234:Zfpm2 UTSW 15 41103074 missense probably damaging 1.00
R9474:Zfpm2 UTSW 15 41103471 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- AAAATCCCAGCGGTCCCTTAG -3'
(R):5'- ATGGCCAATGATGTTTCCAAGTG -3'

Sequencing Primer
(F):5'- CAGCGGTCCCTTAGTGGATG -3'
(R):5'- CCAAGTGCTTAGAGACAATTCCTGG -3'
Posted On 2022-11-14