Mutagenetix > Incidental Mutation

Incidental Mutation 'R0804:Zfp773'

76313

Institutional Source

Beutler Lab

Gene Symbol

Zfp773

Ensembl Gene

ENSMUSG00000063535

Gene Name

zinc finger protein 773

Synonyms

2810409K11Rik

MMRRC Submission

038984-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.052) question?

Stock #

R0804 (G1)

Quality Score

109

Status

Not validated

Chromosome

Chromosomal Location

7133677-7139754 bp(-) (GRCm39)

Type of Mutation

intron

DNA Base Change (assembly)

AGCTGCTGCTGCTGCTGCTGCTGCTGC to AGCTGCTGCTGCTGCTGCTGCTGC at 7136092 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000147639 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000032622] [ENSMUST00000211240]

AlphaFold

Q9CZ29

Predicted Effect

probably benign
Transcript: ENSMUST00000032622

SMART Domains

Protein: ENSMUSP00000032622
Gene: ENSMUSG00000063535

Domain	Start	End	E-Value	Type
KRAB	75	134	6.82e-8	SMART
ZnF_C2H2	241	263	1.31e0	SMART
ZnF_C2H2	269	291	1.5e-4	SMART
ZnF_C2H2	297	319	4.17e-3	SMART
ZnF_C2H2	325	347	2.05e-2	SMART
ZnF_C2H2	353	375	2.24e-3	SMART
ZnF_C2H2	381	403	8.81e-2	SMART
ZnF_C2H2	409	431	7.26e-3	SMART
ZnF_C2H2	437	459	7.26e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000211240

Coding Region Coverage

1x: 99.5%
3x: 99.0%
10x: 97.8%
20x: 96.2%

Validation Efficiency

Allele List at MGI

Other mutations in Zfp773

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00764:Zfp773	APN	7	7,135,683 (GRCm39)	missense	probably damaging	1.00
IGL00780:Zfp773	APN	7	7,136,113 (GRCm39)	missense	probably benign	0.00
IGL01348:Zfp773	APN	7	7,138,314 (GRCm39)	missense	possibly damaging	0.93
IGL02224:Zfp773	APN	7	7,135,975 (GRCm39)	missense	probably benign	0.00
IGL02447:Zfp773	APN	7	7,139,655 (GRCm39)	utr 5 prime	probably benign
IGL02869:Zfp773	APN	7	7,137,232 (GRCm39)	missense	probably benign	0.22
R0505:Zfp773	UTSW	7	7,136,023 (GRCm39)	missense	probably benign	0.03
R0585:Zfp773	UTSW	7	7,135,574 (GRCm39)	missense	probably benign	0.21
R0846:Zfp773	UTSW	7	7,135,691 (GRCm39)	missense	probably damaging	1.00
R1179:Zfp773	UTSW	7	7,136,092 (GRCm39)	intron	probably benign
R2847:Zfp773	UTSW	7	7,136,092 (GRCm39)	intron	probably benign
R3841:Zfp773	UTSW	7	7,135,390 (GRCm39)	missense	possibly damaging	0.92
R4116:Zfp773	UTSW	7	7,136,092 (GRCm39)	intron	probably benign
R4638:Zfp773	UTSW	7	7,138,335 (GRCm39)	missense	probably damaging	1.00
R5126:Zfp773	UTSW	7	7,139,623 (GRCm39)	missense	unknown
R6142:Zfp773	UTSW	7	7,135,481 (GRCm39)	missense	probably benign	0.00
R7072:Zfp773	UTSW	7	7,135,874 (GRCm39)	missense	probably benign	0.15
R7232:Zfp773	UTSW	7	7,135,984 (GRCm39)	missense	probably benign	0.14
R7748:Zfp773	UTSW	7	7,135,907 (GRCm39)	missense	probably benign	0.04
R7888:Zfp773	UTSW	7	7,135,978 (GRCm39)	missense	probably benign	0.00
R8681:Zfp773	UTSW	7	7,139,482 (GRCm39)	missense	possibly damaging	0.70
R8784:Zfp773	UTSW	7	7,135,570 (GRCm39)	missense	probably benign	0.19
R8946:Zfp773	UTSW	7	7,135,469 (GRCm39)	missense	possibly damaging	0.82
R9056:Zfp773	UTSW	7	7,135,989 (GRCm39)	missense	probably damaging	0.99
R9154:Zfp773	UTSW	7	7,138,302 (GRCm39)	missense	probably damaging	0.98
R9295:Zfp773	UTSW	7	7,135,694 (GRCm39)	missense	probably benign	0.06
RF007:Zfp773	UTSW	7	7,135,689 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- TCACATGATGCCTGTGAAGCCC -3'
(R):5'- TAAGGAAGGAAGTTCGGTCCCCAG -3'

Sequencing Primer
(F):5'- CCTGTGAAGCCCGGAGAG -3'
(R):5'- TCAGCCTCATCCCTGGAG -3'

Posted On

2013-10-16