Mutagenetix > Incidental Mutation

Incidental Mutation 'R0788:Tas2r109'

76346

Institutional Source

Beutler Lab

Gene Symbol

Tas2r109

Ensembl Gene

ENSMUSG00000062528

Gene Name

taste receptor, type 2, member 109

Synonyms

mt2r62, T2R09, mGR09, Tas2r9

MMRRC Submission

038968-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.048) question?

Stock #

R0788 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

132956978-132957928 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 132957264 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Leucine at position 222 (Q222L)

Ref Sequence

ENSEMBL: ENSMUSP00000069300 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000067539]

AlphaFold

Q7M707

Predicted Effect

probably benign
Transcript: ENSMUST00000067539
AA Change: Q222L

PolyPhen 2 Score 0.011 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000069300
Gene: ENSMUSG00000062528
AA Change: Q222L

Domain	Start	End	E-Value	Type
Pfam:TAS2R	9	309	4.3e-81	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 96.9%
20x: 93.0%

Validation Efficiency

98% (53/54)

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4833420G17Rik	T	A	13: 119,610,468 (GRCm39)	Y380*	probably null	Het
4931406B18Rik	C	A	7: 43,148,623 (GRCm39)	S196I	probably damaging	Het
Ablim2	T	C	5: 36,015,245 (GRCm39)	S519P	probably benign	Het
Adnp2	A	C	18: 80,173,219 (GRCm39)	C397G	probably benign	Het
Aldh1l2	A	G	10: 83,352,028 (GRCm39)	S156P	probably damaging	Het
Bcl2l15	G	T	3: 103,740,794 (GRCm39)		probably null	Het
Brd9	T	A	13: 74,092,986 (GRCm39)		probably benign	Het
Cars2	A	C	8: 11,579,672 (GRCm39)	I262R	possibly damaging	Het
Ccdc106	T	C	7: 5,060,533 (GRCm39)		probably benign	Het
Cdh3	G	A	8: 107,268,047 (GRCm39)	V361M	probably benign	Het
Cdhr1	A	C	14: 36,809,332 (GRCm39)		probably null	Het
Cdk5rap2	A	G	4: 70,225,468 (GRCm39)	I559T	possibly damaging	Het
Cdkn2aip	T	A	8: 48,166,798 (GRCm39)	Q3L	possibly damaging	Het
Chd1	G	T	17: 15,927,376 (GRCm39)	V10F	possibly damaging	Het
Col4a4	G	A	1: 82,502,717 (GRCm39)	P356S	unknown	Het
Col6a4	T	C	9: 105,949,197 (GRCm39)	K813E	probably benign	Het
Cttnbp2	G	A	6: 18,423,834 (GRCm39)	T830I	probably damaging	Het
Cyp2t4	A	G	7: 26,854,588 (GRCm39)	M23V	probably null	Het
Cyp3a16	T	C	5: 145,401,886 (GRCm39)	K59E	probably benign	Het
Dpysl5	G	A	5: 30,946,185 (GRCm39)		probably null	Het
E130308A19Rik	T	A	4: 59,719,847 (GRCm39)	Y460N	possibly damaging	Het
Ear6	T	A	14: 52,091,487 (GRCm39)	C11*	probably null	Het
Fat1	C	T	8: 45,477,020 (GRCm39)	T1999M	probably benign	Het
Gsdmd	T	A	15: 75,736,103 (GRCm39)	C77*	probably null	Het
Hsp90ab1	ACTTCTT	ACTT	17: 45,880,425 (GRCm39)		probably benign	Het
Kif28	A	T	1: 179,532,788 (GRCm39)		probably benign	Het
Krt6b	A	G	15: 101,585,954 (GRCm39)	I373T	probably damaging	Het
Lgr5	T	C	10: 115,288,902 (GRCm39)	T509A	probably damaging	Het
Mapkbp1	C	T	2: 119,854,482 (GRCm39)	P1354S	probably benign	Het
Nat10	A	G	2: 103,573,460 (GRCm39)	S346P	probably damaging	Het
Ncoa2	T	C	1: 13,237,113 (GRCm39)		probably benign	Het
Necap1	C	T	6: 122,858,495 (GRCm39)	R113W	probably damaging	Het
Or1l4	T	A	2: 37,092,035 (GRCm39)	Y261N	possibly damaging	Het
Orc4	A	T	2: 48,827,479 (GRCm39)	V38E	possibly damaging	Het
Per1	G	A	11: 68,992,185 (GRCm39)		probably benign	Het
Polb	T	C	8: 23,132,354 (GRCm39)	D130G	probably null	Het
Ppcs	T	C	4: 119,279,375 (GRCm39)	N59S	probably damaging	Het
Ppp2ca	A	G	11: 52,003,969 (GRCm39)	E42G	possibly damaging	Het
Ptprf	T	C	4: 118,083,663 (GRCm39)	T807A	probably damaging	Het
Rapgef2	A	C	3: 79,006,502 (GRCm39)	F284V	possibly damaging	Het
Sestd1	A	T	2: 77,022,060 (GRCm39)	F544I	probably damaging	Het
Slfn3	G	A	11: 83,103,662 (GRCm39)	G178S	possibly damaging	Het
Supt6	G	A	11: 78,098,598 (GRCm39)		probably benign	Het
Tekt4	A	C	17: 25,691,021 (GRCm39)	D109A	probably damaging	Het
Tob2	C	A	15: 81,735,903 (GRCm39)	R22L	probably damaging	Het
Ttn	T	G	2: 76,653,282 (GRCm39)	E178D	possibly damaging	Het
Ube2u	C	A	4: 100,371,937 (GRCm39)		probably benign	Het
Uggt2	A	G	14: 119,332,812 (GRCm39)		probably benign	Het
Vstm2a	T	C	11: 16,209,968 (GRCm39)	F65L	probably damaging	Het
Zfp57	A	G	17: 37,317,092 (GRCm39)		probably benign	Het
Znrf3	G	T	11: 5,231,320 (GRCm39)	P731Q	probably benign	Het

Other mutations in Tas2r109

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00467:Tas2r109	APN	6	132,956,986 (GRCm39)	missense	probably benign	0.04
IGL01837:Tas2r109	APN	6	132,957,477 (GRCm39)	missense	probably benign	0.27
IGL02094:Tas2r109	APN	6	132,957,202 (GRCm39)	missense	possibly damaging	0.78
R0849:Tas2r109	UTSW	6	132,957,856 (GRCm39)	missense	probably benign	0.00
R1542:Tas2r109	UTSW	6	132,957,873 (GRCm39)	missense	possibly damaging	0.93
R1583:Tas2r109	UTSW	6	132,957,389 (GRCm39)	missense	probably benign	0.01
R2035:Tas2r109	UTSW	6	132,957,423 (GRCm39)	missense	probably benign
R3845:Tas2r109	UTSW	6	132,957,766 (GRCm39)	missense	probably damaging	0.99
R4060:Tas2r109	UTSW	6	132,957,148 (GRCm39)	missense	probably damaging	1.00
R4355:Tas2r109	UTSW	6	132,957,144 (GRCm39)	missense	probably benign
R5353:Tas2r109	UTSW	6	132,957,594 (GRCm39)	missense	possibly damaging	0.61
R5860:Tas2r109	UTSW	6	132,957,664 (GRCm39)	missense	probably benign	0.06
R6211:Tas2r109	UTSW	6	132,957,587 (GRCm39)	nonsense	probably null
R6378:Tas2r109	UTSW	6	132,957,844 (GRCm39)	missense	probably benign	0.00
R6861:Tas2r109	UTSW	6	132,957,048 (GRCm39)	missense	probably benign	0.43
R7319:Tas2r109	UTSW	6	132,957,663 (GRCm39)	missense	probably benign	0.33
R8553:Tas2r109	UTSW	6	132,957,171 (GRCm39)	missense	probably benign	0.33
R9447:Tas2r109	UTSW	6	132,957,270 (GRCm39)	missense	probably damaging	1.00
Z1088:Tas2r109	UTSW	6	132,957,264 (GRCm39)	missense	probably benign	0.07

Predicted Primers

PCR Primer
(F):5'- TTTGGCCTGCATTTCAGCCACG -3'
(R):5'- TGGTTTGCTACATGCCTCAGTGTC -3'

Sequencing Primer
(F):5'- GGAAAAGCTGTATCTACAACCTG -3'
(R):5'- AGTTTCAGTTACATTACTGGTGTCTC -3'

Posted On

2013-10-16