Mutagenetix > Incidental Mutation

Incidental Mutation 'R0790:Ccdc150'

76421

Institutional Source

Beutler Lab

Gene Symbol

Ccdc150

Ensembl Gene

ENSMUSG00000025983

Gene Name

coiled-coil domain containing 150

Synonyms

4930511H11Rik

MMRRC Submission

038970-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.082) question?

Stock #

R0790 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

54250683-54368727 bp(+) (GRCm38)

Type of Mutation

splice site

DNA Base Change (assembly)

T to A at 54277776 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000125195 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000027128] [ENSMUST00000160472]

AlphaFold

Q8CDI7

Predicted Effect

probably benign
Transcript: ENSMUST00000027128

SMART Domains

Protein: ENSMUSP00000027128
Gene: ENSMUSG00000025983

Domain	Start	End	E-Value	Type
coiled coil region	160	250	N/A	INTRINSIC
coiled coil region	288	314	N/A	INTRINSIC
coiled coil region	418	676	N/A	INTRINSIC
coiled coil region	727	952	N/A	INTRINSIC
coiled coil region	985	1048	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000160472

SMART Domains

Protein: ENSMUSP00000125195
Gene: ENSMUSG00000025983

Domain	Start	End	E-Value	Type
coiled coil region	160	250	N/A	INTRINSIC
coiled coil region	288	314	N/A	INTRINSIC
coiled coil region	418	551	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000161988

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 96.8%
20x: 92.0%

Validation Efficiency

100% (41/41)

Allele List at MGI

Other mutations in this stock

Total: 34 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Apob	A	G	12: 8,010,245 (GRCm38)	E2876G	probably damaging	Het
Arhgef28	A	G	13: 97,981,406 (GRCm38)	S585P	possibly damaging	Het
Atp11b	T	A	3: 35,832,923 (GRCm38)	N685K	probably damaging	Het
Camsap2	C	T	1: 136,273,737 (GRCm38)		probably benign	Het
Cdc23	A	G	18: 34,651,613 (GRCm38)	I32T	possibly damaging	Het
Cdca2	C	T	14: 67,680,291 (GRCm38)	D553N	probably benign	Het
Chd8	T	C	14: 52,204,025 (GRCm38)	D870G	probably benign	Het
Chit1	T	G	1: 134,138,752 (GRCm38)	V2G	probably benign	Het
Col4a4	G	A	1: 82,524,996 (GRCm38)	P356S	unknown	Het
Cpsf4l	A	G	11: 113,706,408 (GRCm38)		probably benign	Het
Etaa1	G	T	11: 17,946,051 (GRCm38)	Q689K	probably benign	Het
Fam83h	C	T	15: 76,003,392 (GRCm38)	V699I	probably benign	Het
Fry	A	G	5: 150,466,437 (GRCm38)	D2545G	probably benign	Het
Hsp90ab1	ACTTCTT	ACTT	17: 45,569,499 (GRCm38)		probably benign	Het
Kif5a	T	C	10: 127,246,009 (GRCm38)		probably benign	Het
Lifr	A	G	15: 7,185,715 (GRCm38)	T768A	probably benign	Het
Mrap2	T	C	9: 87,182,782 (GRCm38)	L192P	possibly damaging	Het
Myo18a	G	A	11: 77,840,709 (GRCm38)	R840Q	possibly damaging	Het
Myrfl	T	C	10: 116,817,788 (GRCm38)	D458G	probably damaging	Het
Or12j4	T	C	7: 140,467,049 (GRCm38)	S283P	possibly damaging	Het
Or51s1	A	G	7: 102,909,636 (GRCm38)	Y68H	probably benign	Het
Pcdh15	A	G	10: 74,631,053 (GRCm38)	E1034G	probably benign	Het
Pirb	A	T	7: 3,717,638 (GRCm38)	L287Q	probably benign	Het
Plcb2	G	A	2: 118,712,483 (GRCm38)		probably benign	Het
Psmd1	T	C	1: 86,077,450 (GRCm38)	V202A	possibly damaging	Het
Raet1d	T	C	10: 22,370,896 (GRCm38)	I41T	probably damaging	Het
Retnlb	T	A	16: 48,817,226 (GRCm38)	L5Q	possibly damaging	Het
Sh3rf1	T	C	8: 61,329,258 (GRCm38)	Y145H	probably damaging	Het
Smad7	C	T	18: 75,393,862 (GRCm38)	H260Y	probably benign	Het
Stk10	T	C	11: 32,598,653 (GRCm38)	V407A	probably benign	Het
Synpo	A	G	18: 60,603,503 (GRCm38)	V218A	probably damaging	Het
Tll2	T	A	19: 41,103,850 (GRCm38)	Y499F	probably damaging	Het
Vrk1	C	T	12: 106,070,624 (GRCm38)	P390S	probably benign	Het
Zfp748	A	C	13: 67,545,362 (GRCm38)	L64W	probably benign	Het

Other mutations in Ccdc150

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00712:Ccdc150	APN	1	54,272,550 (GRCm38)	splice site	probably benign
IGL00819:Ccdc150	APN	1	54,263,573 (GRCm38)	missense	probably damaging	1.00
IGL01973:Ccdc150	APN	1	54,300,488 (GRCm38)	splice site	probably null
IGL02352:Ccdc150	APN	1	54,272,521 (GRCm38)	missense	probably benign	0.25
IGL02359:Ccdc150	APN	1	54,272,521 (GRCm38)	missense	probably benign	0.25
IGL02620:Ccdc150	APN	1	54,263,545 (GRCm38)	nonsense	probably null
IGL02673:Ccdc150	APN	1	54,328,990 (GRCm38)	missense	probably benign	0.09
IGL03148:Ccdc150	APN	1	54,278,715 (GRCm38)	missense	possibly damaging	0.68
IGL03185:Ccdc150	APN	1	54,300,323 (GRCm38)	missense	probably damaging	1.00
IGL03014:Ccdc150	UTSW	1	54,290,702 (GRCm38)	missense	probably damaging	0.99
R0066:Ccdc150	UTSW	1	54,356,691 (GRCm38)	missense	probably benign
R0066:Ccdc150	UTSW	1	54,356,691 (GRCm38)	missense	probably benign
R0217:Ccdc150	UTSW	1	54,300,430 (GRCm38)	missense	possibly damaging	0.87
R0582:Ccdc150	UTSW	1	54,329,511 (GRCm38)	missense	probably benign
R0687:Ccdc150	UTSW	1	54,285,631 (GRCm38)	splice site	probably null
R1146:Ccdc150	UTSW	1	54,364,971 (GRCm38)	splice site	probably benign
R1288:Ccdc150	UTSW	1	54,364,458 (GRCm38)	missense	probably damaging	1.00
R1763:Ccdc150	UTSW	1	54,354,636 (GRCm38)	missense	probably benign	0.42
R1855:Ccdc150	UTSW	1	54,367,910 (GRCm38)	intron	probably benign
R1957:Ccdc150	UTSW	1	54,263,909 (GRCm38)	missense	probably benign	0.00
R2180:Ccdc150	UTSW	1	54,272,547 (GRCm38)	critical splice donor site	probably null
R2226:Ccdc150	UTSW	1	54,364,925 (GRCm38)	missense	probably null	0.11
R3054:Ccdc150	UTSW	1	54,288,842 (GRCm38)	missense	possibly damaging	0.51
R3055:Ccdc150	UTSW	1	54,288,842 (GRCm38)	missense	possibly damaging	0.51
R3056:Ccdc150	UTSW	1	54,288,842 (GRCm38)	missense	possibly damaging	0.51
R3409:Ccdc150	UTSW	1	54,356,773 (GRCm38)	missense	probably benign	0.02
R3411:Ccdc150	UTSW	1	54,356,773 (GRCm38)	missense	probably benign	0.02
R3812:Ccdc150	UTSW	1	54,368,310 (GRCm38)	missense	probably benign	0.00
R4031:Ccdc150	UTSW	1	54,278,811 (GRCm38)	missense	probably benign	0.31
R4356:Ccdc150	UTSW	1	54,353,054 (GRCm38)	missense	probably damaging	0.98
R4617:Ccdc150	UTSW	1	54,355,754 (GRCm38)	missense	probably benign	0.00
R4757:Ccdc150	UTSW	1	54,278,715 (GRCm38)	missense	possibly damaging	0.81
R4957:Ccdc150	UTSW	1	54,364,868 (GRCm38)	intron	probably benign
R5028:Ccdc150	UTSW	1	54,263,477 (GRCm38)	missense	probably benign	0.01
R5512:Ccdc150	UTSW	1	54,354,647 (GRCm38)	missense	probably damaging	0.96
R5757:Ccdc150	UTSW	1	54,263,620 (GRCm38)	missense	probably damaging	1.00
R5943:Ccdc150	UTSW	1	54,300,367 (GRCm38)	missense	probably benign	0.01
R5948:Ccdc150	UTSW	1	54,277,714 (GRCm38)	missense	possibly damaging	0.79
R6033:Ccdc150	UTSW	1	54,285,628 (GRCm38)	critical splice donor site	probably null
R6033:Ccdc150	UTSW	1	54,285,628 (GRCm38)	critical splice donor site	probably null
R6065:Ccdc150	UTSW	1	54,263,599 (GRCm38)	missense	possibly damaging	0.90
R6390:Ccdc150	UTSW	1	54,368,017 (GRCm38)	missense	probably benign	0.01
R6399:Ccdc150	UTSW	1	54,263,957 (GRCm38)	splice site	probably null
R6988:Ccdc150	UTSW	1	54,355,709 (GRCm38)	nonsense	probably null
R7248:Ccdc150	UTSW	1	54,304,898 (GRCm38)	missense	probably benign	0.00
R7319:Ccdc150	UTSW	1	54,263,337 (GRCm38)	splice site	probably null
R7322:Ccdc150	UTSW	1	54,259,966 (GRCm38)	missense	probably benign	0.01
R7366:Ccdc150	UTSW	1	54,300,382 (GRCm38)	nonsense	probably null
R7647:Ccdc150	UTSW	1	54,356,704 (GRCm38)	missense	probably damaging	1.00
R7981:Ccdc150	UTSW	1	54,368,392 (GRCm38)	missense	probably damaging	1.00
R8002:Ccdc150	UTSW	1	54,272,497 (GRCm38)	missense	probably damaging	0.99
R8201:Ccdc150	UTSW	1	54,329,487 (GRCm38)	missense	probably benign	0.10
R8688:Ccdc150	UTSW	1	54,367,973 (GRCm38)	missense	probably damaging	1.00
R8719:Ccdc150	UTSW	1	54,263,509 (GRCm38)	missense	probably benign	0.00
R8963:Ccdc150	UTSW	1	54,272,482 (GRCm38)	missense	probably benign	0.14
R9178:Ccdc150	UTSW	1	54,272,485 (GRCm38)	missense	probably damaging	0.99
R9200:Ccdc150	UTSW	1	54,260,038 (GRCm38)	missense	probably damaging	1.00
R9332:Ccdc150	UTSW	1	54,277,751 (GRCm38)	missense	probably damaging	0.99
R9367:Ccdc150	UTSW	1	54,285,601 (GRCm38)	missense	probably damaging	1.00
R9416:Ccdc150	UTSW	1	54,278,831 (GRCm38)	missense	probably damaging	0.97
R9430:Ccdc150	UTSW	1	54,281,771 (GRCm38)	missense	probably damaging	1.00
R9576:Ccdc150	UTSW	1	54,368,385 (GRCm38)	nonsense	probably null
R9747:Ccdc150	UTSW	1	54,259,948 (GRCm38)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- TGGGCTGACAAACCTTGTAAACTGC -3'
(R):5'- TGCCTCTATAAGACCTGTGACAGACC -3'

Sequencing Primer
(F):5'- AACCTTGTAAACTGCCATGCTG -3'
(R):5'- tgtgacagacccagttttcc -3'

Posted On

2013-10-16