Incidental Mutation 'R0780:Ptgds'
ID 76575
Institutional Source Beutler Lab
Gene Symbol Ptgds
Ensembl Gene ENSMUSG00000015090
Gene Name prostaglandin D2 synthase (brain)
Synonyms L-PGDS, PGD2, Ptgs3
MMRRC Submission 038960-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.093) question?
Stock # R0780 (G1)
Quality Score 225
Status Not validated
Chromosome 2
Chromosomal Location 25356721-25360058 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to G at 25358104 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Phenylalanine to Serine at position 143 (F143S)
Ref Sequence ENSEMBL: ENSMUSP00000109897 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000015234] [ENSMUST00000114251] [ENSMUST00000114259]
AlphaFold O09114
Predicted Effect possibly damaging
Transcript: ENSMUST00000015234
AA Change: F143S

PolyPhen 2 Score 0.903 (Sensitivity: 0.82; Specificity: 0.94)
SMART Domains Protein: ENSMUSP00000015234
Gene: ENSMUSG00000015090
AA Change: F143S

DomainStartEndE-ValueType
low complexity region 4 18 N/A INTRINSIC
Pfam:Lipocalin 40 184 4.2e-35 PFAM
Predicted Effect possibly damaging
Transcript: ENSMUST00000114251
AA Change: F143S

PolyPhen 2 Score 0.903 (Sensitivity: 0.82; Specificity: 0.94)
SMART Domains Protein: ENSMUSP00000109889
Gene: ENSMUSG00000015090
AA Change: F143S

DomainStartEndE-ValueType
low complexity region 4 18 N/A INTRINSIC
Pfam:Lipocalin 40 184 4.2e-35 PFAM
Predicted Effect possibly damaging
Transcript: ENSMUST00000114259
AA Change: F143S

PolyPhen 2 Score 0.903 (Sensitivity: 0.82; Specificity: 0.94)
SMART Domains Protein: ENSMUSP00000109897
Gene: ENSMUSG00000015090
AA Change: F143S

DomainStartEndE-ValueType
low complexity region 4 18 N/A INTRINSIC
Pfam:Lipocalin 40 184 4.2e-35 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000137417
Predicted Effect noncoding transcript
Transcript: ENSMUST00000144016
Coding Region Coverage
  • 1x: 99.4%
  • 3x: 98.8%
  • 10x: 97.4%
  • 20x: 94.8%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a glutathione-independent prostaglandin D synthase that catalyzes the conversion of prostaglandin H2 (PGH2) to postaglandin D2 (PGD2). PGD2 functions as a neuromodulator as well as a trophic factor in the central nervous system. PGD2 is also involved in smooth muscle contraction/relaxation and is a potent inhibitor of platelet aggregation. This gene is preferentially expressed in brain. Studies with transgenic mice overexpressing this gene suggest that this gene may be also involved in the regulation of non-rapid eye movement sleep. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for one knock-out allele fail to exhibit PGE2- and bicuculline-induced allodynia and exhibit decreased susceptibility to IgE-induced PCA. Mice homozygous for another knock-out allele show normal induction of muscle injury after reperfusion of ischemic skeletal muscle. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 24 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Ache C T 5: 137,288,794 (GRCm39) R167C probably damaging Het
Ahsa1 T C 12: 87,315,102 (GRCm39) I85T probably benign Het
Btaf1 T C 19: 36,966,322 (GRCm39) L1030S probably damaging Het
Ccdc163 A G 4: 116,569,604 (GRCm39) K222E probably benign Het
Cpsf1 A G 15: 76,484,577 (GRCm39) F635L probably benign Het
Cubn G A 2: 13,461,424 (GRCm39) T701M probably damaging Het
Cxcr2 T A 1: 74,198,334 (GRCm39) M276K probably damaging Het
Daam1 T A 12: 71,993,824 (GRCm39) I409K unknown Het
Dnah10 G T 5: 124,827,876 (GRCm39) G741W possibly damaging Het
Ica1l A G 1: 60,036,608 (GRCm39) probably null Het
Ifi208 A G 1: 173,510,262 (GRCm39) D139G probably benign Het
Kat2b T A 17: 53,874,476 (GRCm39) V40E unknown Het
Kmt2d G T 15: 98,760,738 (GRCm39) P871T unknown Het
Lats2 A T 14: 57,928,753 (GRCm39) Y1041N probably damaging Het
Lifr C T 15: 7,206,947 (GRCm39) T486I probably benign Het
Mtmr4 T A 11: 87,502,266 (GRCm39) D773E probably benign Het
Rp1l1 A G 14: 64,267,800 (GRCm39) S1129G possibly damaging Het
Sdk2 A G 11: 113,784,334 (GRCm39) V135A probably benign Het
Slc12a8 G A 16: 33,467,035 (GRCm39) probably null Het
Thsd7a A G 6: 12,337,273 (GRCm39) V1248A probably damaging Het
Tpr T A 1: 150,307,092 (GRCm39) H1562Q probably benign Het
Uba3 G A 6: 97,163,666 (GRCm39) R294* probably null Het
Vmn2r22 A T 6: 123,614,933 (GRCm39) V219E probably damaging Het
Zfand6 T A 7: 84,265,042 (GRCm39) I220F probably damaging Het
Other mutations in Ptgds
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02164:Ptgds APN 2 25,359,124 (GRCm39) missense probably damaging 0.99
IGL03035:Ptgds APN 2 25,359,622 (GRCm39) missense probably benign 0.06
IGL03036:Ptgds APN 2 25,359,622 (GRCm39) missense probably benign 0.06
IGL03117:Ptgds APN 2 25,359,622 (GRCm39) missense probably benign 0.06
IGL03352:Ptgds APN 2 25,359,622 (GRCm39) missense probably benign 0.06
IGL03354:Ptgds APN 2 25,359,622 (GRCm39) missense probably benign 0.06
R0885:Ptgds UTSW 2 25,357,357 (GRCm39) missense possibly damaging 0.80
R4820:Ptgds UTSW 2 25,359,058 (GRCm39) missense probably benign 0.02
R7000:Ptgds UTSW 2 25,357,828 (GRCm39) critical splice donor site probably null
R7522:Ptgds UTSW 2 25,357,920 (GRCm39) missense probably benign 0.38
R8401:Ptgds UTSW 2 25,359,669 (GRCm39) missense unknown
R9794:Ptgds UTSW 2 25,359,129 (GRCm39) missense probably benign 0.05
Predicted Primers PCR Primer
(F):5'- TGACTATAATTCCCTCCCCAGCAGC -3'
(R):5'- TGGATGAAGAAGACCTGAGACCCC -3'

Sequencing Primer
(F):5'- GGAAAACAATGTCCTCCTCTGTG -3'
(R):5'- CAGAGAAGCCCCCAAAGG -3'
Posted On 2013-10-16