Mutagenetix > Incidental Mutation

Incidental Mutation 'R0783:Olfr1224-ps1'

76708

Institutional Source

Beutler Lab

Gene Symbol

Olfr1224-ps1

Ensembl Gene

ENSMUSG00000075099

Gene Name

olfactory receptor 1224, pseudogene 1

Synonyms

GA_x6K02T2Q125-50635980-50635046, MOR233-15

MMRRC Submission

038963-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.066) question?

Stock #

R0783 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

89155336-89164091 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 89156891 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 95 (C95S)

Ref Sequence

ENSEMBL: ENSMUSP00000148938 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099792] [ENSMUST00000099793] [ENSMUST00000216833] [ENSMUST00000216976]

AlphaFold

A3KGY4

Predicted Effect

probably benign
Transcript: ENSMUST00000099792
AA Change: C95S

PolyPhen 2 Score 0.297 (Sensitivity: 0.91; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000097380
Gene: ENSMUSG00000075099
AA Change: C95S

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	303	1.1e-48	PFAM
Pfam:7tm_1	39	287	9.9e-17	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000099793
AA Change: C95S

PolyPhen 2 Score 0.297 (Sensitivity: 0.91; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000097381
Gene: ENSMUSG00000075099
AA Change: C95S

Domain	Start	End	E-Value	Type
Pfam:7tm_1	39	286	2e-26	PFAM
Pfam:7tm_4	138	283	5.6e-37	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000214069

Predicted Effect

probably benign
Transcript: ENSMUST00000216833
AA Change: C95S

PolyPhen 2 Score 0.297 (Sensitivity: 0.91; Specificity: 0.89)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000216913

Predicted Effect

probably benign
Transcript: ENSMUST00000216976
AA Change: C95S

PolyPhen 2 Score 0.297 (Sensitivity: 0.91; Specificity: 0.89)

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 96.8%
20x: 92.2%

Validation Efficiency

100% (47/47)

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca14	G	A	7: 120,294,157	G1277R	probably damaging	Het
Abi3bp	A	G	16: 56,595,238		probably null	Het
Acsm2	G	A	7: 119,573,117	G61D	probably damaging	Het
Akp3	G	A	1: 87,127,871	G547R	unknown	Het
Bbs9	T	C	9: 22,567,714	L151S	possibly damaging	Het
Camk2g	T	A	14: 20,744,636	T173S	possibly damaging	Het
Eif3b	T	C	5: 140,419,837		probably benign	Het
Eif3i	A	C	4: 129,592,076	F319V	possibly damaging	Het
Eprs	T	C	1: 185,398,458	L672P	probably damaging	Het
Fras1	C	T	5: 96,768,430	A3441V	probably damaging	Het
Gigyf2	T	A	1: 87,407,161	M79K	probably damaging	Het
Grrp1	G	A	4: 134,252,057	R37C	probably damaging	Het
Hdac1	T	C	4: 129,518,109	N331S	probably benign	Het
Hmcn1	C	T	1: 150,650,073	G3300S	probably damaging	Het
Iars2	T	C	1: 185,320,874	E400G	probably damaging	Het
Irx2	T	C	13: 72,632,650		probably null	Het
Itih4	G	A	14: 30,895,423	E567K	possibly damaging	Het
Klhl5	T	A	5: 65,156,253		probably benign	Het
Klk8	G	A	7: 43,802,197	G204E	probably damaging	Het
Loxhd1	T	C	18: 77,429,984	F1843L	possibly damaging	Het
Mllt6	T	C	11: 97,665,745	V87A	probably damaging	Het
Mylk	G	C	16: 34,879,475	E403Q	possibly damaging	Het
Nhlrc3	A	T	3: 53,462,449	S34T	probably benign	Het
Olfr1099	A	G	2: 86,958,562	C299R	probably benign	Het
Olfr1494	T	C	19: 13,749,676	L190P	probably damaging	Het
Olfr193	A	T	16: 59,110,169	L147*	probably null	Het
Olfr549	A	G	7: 102,554,439	I52V	probably benign	Het
Olfr593	T	C	7: 103,212,670	F259S	probably damaging	Het
Pcnx4	T	C	12: 72,575,478	W1074R	probably damaging	Het
Pcsk9	T	C	4: 106,450,117	T310A	probably benign	Het
Pfas	A	G	11: 69,000,521	L250P	probably damaging	Het
Plk5	T	A	10: 80,361,130	D352E	probably benign	Het
Ryr3	A	G	2: 112,756,327		probably benign	Het
Serinc3	A	G	2: 163,637,003	I68T	possibly damaging	Het
Sez6l2	A	G	7: 126,967,145	T810A	possibly damaging	Het
Tmprss7	G	A	16: 45,667,606	Q487*	probably null	Het
Tnf	A	G	17: 35,201,674	I56T	probably damaging	Het
Ttbk2	A	T	2: 120,739,977	S1163T	possibly damaging	Het
Ttn	G	A	2: 76,743,530	T23927I	probably damaging	Het
Urb1	G	A	16: 90,810,297	A15V	possibly damaging	Het
Zfp128	C	T	7: 12,890,272	P189L	probably damaging	Het
Zfr	T	C	15: 12,162,182	V806A	probably damaging	Het

Other mutations in Olfr1224-ps1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01670:Olfr1224-ps1	APN	2	89156917	missense	probably benign	0.01
IGL02561:Olfr1224-ps1	APN	2	89157141	missense	possibly damaging	0.94
R0086:Olfr1224-ps1	UTSW	2	89156476	missense	probably benign	0.01
R0096:Olfr1224-ps1	UTSW	2	89156296	missense	probably benign	0.03
R0096:Olfr1224-ps1	UTSW	2	89156296	missense	probably benign	0.03
R1920:Olfr1224-ps1	UTSW	2	89156581	missense	probably benign	0.44
R1921:Olfr1224-ps1	UTSW	2	89156581	missense	probably benign	0.44
R2033:Olfr1224-ps1	UTSW	2	89157154	missense	probably damaging	0.96
R3500:Olfr1224-ps1	UTSW	2	89157059	missense	probably damaging	1.00
R5044:Olfr1224-ps1	UTSW	2	89156939	nonsense	probably null
R5140:Olfr1224-ps1	UTSW	2	89157107	missense	probably benign	0.12
R5253:Olfr1224-ps1	UTSW	2	89156457	nonsense	probably null
R6338:Olfr1224-ps1	UTSW	2	89156371	missense	probably damaging	1.00
R6431:Olfr1224-ps1	UTSW	2	89157161	missense	probably damaging	1.00
R6904:Olfr1224-ps1	UTSW	2	89156813	missense	possibly damaging	0.57
R7259:Olfr1224-ps1	UTSW	2	89156510	missense	probably benign	0.03
R7820:Olfr1224-ps1	UTSW	2	89156248	missense	probably benign	0.08
R9026:Olfr1224-ps1	UTSW	2	89156644	missense	probably benign	0.22
R9076:Olfr1224-ps1	UTSW	2	89156375	missense	possibly damaging	0.95
R9266:Olfr1224-ps1	UTSW	2	89156510	missense	possibly damaging	0.70
R9378:Olfr1224-ps1	UTSW	2	89157055	missense	probably damaging	1.00
R9580:Olfr1224-ps1	UTSW	2	89157121	missense	probably benign	0.20
R9751:Olfr1224-ps1	UTSW	2	89156438	missense	possibly damaging	0.91
Z1176:Olfr1224-ps1	UTSW	2	89156467	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CAAGCTATTCCGACTAGGATGCCAC -3'
(R):5'- GCAAAACCAGAGCATTGTCACTGAG -3'

Sequencing Primer
(F):5'- TAGGATGCCACAGAGCCTC -3'
(R):5'- TGCTGGGACTTTCACAGAAC -3'

Posted On

2013-10-16