Mutagenetix > Incidental Mutation

Incidental Mutation 'R0786:Vmn2r104'

76880

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r104

Ensembl Gene

ENSMUSG00000090315

Gene Name

vomeronasal 2, receptor 104

Synonyms

V2r7

MMRRC Submission

038966-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.097) question?

Stock #

R0786 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

20029425-20048205 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 20042725 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Lysine at position 158 (I158K)

Ref Sequence

ENSEMBL: ENSMUSP00000129895 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000168050]

AlphaFold

E9Q2J5

Predicted Effect

probably benign
Transcript: ENSMUST00000168050
AA Change: I158K

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000129895
Gene: ENSMUSG00000090315
AA Change: I158K

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	85	457	4e-38	PFAM
Pfam:NCD3G	512	565	2.1e-20	PFAM
Pfam:7tm_3	598	833	1.7e-52	PFAM

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 96.6%
20x: 92.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adrb3	T	C	8: 27,226,852		probably benign	Het
Atg2b	T	C	12: 105,636,508	H1592R	probably benign	Het
Bpifb1	C	T	2: 154,202,661	A16V	probably benign	Het
Casc1	A	G	6: 145,181,757		probably null	Het
Cep57	T	A	9: 13,809,870	Y271F	probably damaging	Het
Cfap54	A	T	10: 92,967,535	N1548K	possibly damaging	Het
Chrna6	A	T	8: 27,408,380	D103E	probably benign	Het
Clec12b	T	C	6: 129,380,688	N69S	probably benign	Het
Col27a1	T	C	4: 63,291,578		probably null	Het
Cwh43	T	A	5: 73,408,183	Y30*	probably null	Het
Dsg4	T	C	18: 20,449,372		probably null	Het
Efna3	T	C	3: 89,316,573	N103S	probably damaging	Het
Efr3a	A	G	15: 65,853,551	D532G	possibly damaging	Het
Fam227a	A	T	15: 79,626,268	V395D	probably benign	Het
Fes	T	A	7: 80,386,920	D93V	probably damaging	Het
Gbp3	T	A	3: 142,570,971	M510K	possibly damaging	Het
Gpr12	A	T	5: 146,583,504	S44T	probably damaging	Het
Gpr179	T	C	11: 97,343,274	N583S	probably damaging	Het
Hipk1	T	C	3: 103,744,304	T1093A	probably benign	Het
Hspb1	T	C	5: 135,889,243	L148P	probably damaging	Het
Kirrel3	A	G	9: 35,034,865	N640S	probably damaging	Het
Mak	G	T	13: 41,046,069	Q365K	probably benign	Het
Melk	A	G	4: 44,303,649	Y14C	unknown	Het
Myh7	T	C	14: 54,992,873	M1V	probably null	Het
Nsmf	A	G	2: 25,060,510	Y330C	probably damaging	Het
Parp14	A	G	16: 35,840,802	F1592S	possibly damaging	Het
Pnpla6	A	T	8: 3,523,317	I394F	probably benign	Het
Ppl	T	C	16: 5,089,054	R1126G	probably damaging	Het
Prss8	C	T	7: 127,926,474	R291Q	probably benign	Het
Ptpro	T	A	6: 137,443,594	V1007D	probably damaging	Het
Rasgrp1	G	T	2: 117,300,499	D155E	probably benign	Het
Recql5	T	C	11: 115,895,802	I563V	probably benign	Het
Rhbg	C	T	3: 88,244,568	M394I	probably benign	Het
Rnf130	A	G	11: 50,087,437	D275G	probably damaging	Het
Smg9	T	C	7: 24,420,864	F421S	probably benign	Het
Tgm2	T	C	2: 158,124,381	E451G	probably damaging	Het
Tle1	T	A	4: 72,199,361	T21S	probably damaging	Het
Tmc5	T	C	7: 118,627,210	I266T	possibly damaging	Het
Tmigd3	T	A	3: 105,917,002	C96S	probably damaging	Het
Trdn	A	T	10: 33,305,081	T361S	probably benign	Het
Vgll3	A	T	16: 65,860,682	Q261L	probably benign	Het
Zfp964	A	G	8: 69,664,081	K444E	possibly damaging	Het

Other mutations in Vmn2r104

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00427:Vmn2r104	APN	17	20038239	missense	probably damaging	0.98
IGL01098:Vmn2r104	APN	17	20048096	missense	probably benign	0.27
IGL01333:Vmn2r104	APN	17	20042793	missense	probably benign	0.17
IGL01527:Vmn2r104	APN	17	20042896	missense	possibly damaging	0.82
IGL01773:Vmn2r104	APN	17	20040668	missense	probably benign	0.10
IGL01939:Vmn2r104	APN	17	20029925	missense	probably damaging	0.99
IGL02121:Vmn2r104	APN	17	20041794	nonsense	probably null
IGL02305:Vmn2r104	APN	17	20042856	missense	probably benign	0.09
IGL02374:Vmn2r104	APN	17	20042786	missense	probably benign	0.34
IGL03260:Vmn2r104	APN	17	20042821	missense	probably benign	0.05
IGL03366:Vmn2r104	APN	17	20029604	missense	probably damaging	1.00
R0091:Vmn2r104	UTSW	17	20041813	missense	possibly damaging	0.79
R0125:Vmn2r104	UTSW	17	20029807	missense	probably damaging	0.98
R0257:Vmn2r104	UTSW	17	20029627	missense	probably damaging	1.00
R0381:Vmn2r104	UTSW	17	20048002	nonsense	probably null
R0709:Vmn2r104	UTSW	17	20042904	missense	probably damaging	1.00
R1575:Vmn2r104	UTSW	17	20042215	missense	probably damaging	1.00
R1827:Vmn2r104	UTSW	17	20042235	missense	probably damaging	0.97
R1932:Vmn2r104	UTSW	17	20040769	missense	probably damaging	1.00
R1956:Vmn2r104	UTSW	17	20042051	missense	probably damaging	0.98
R2203:Vmn2r104	UTSW	17	20029821	missense	probably benign	0.05
R2205:Vmn2r104	UTSW	17	20029821	missense	probably benign	0.05
R2859:Vmn2r104	UTSW	17	20048193	missense	possibly damaging	0.82
R3701:Vmn2r104	UTSW	17	20029556	missense	probably damaging	1.00
R3834:Vmn2r104	UTSW	17	20029921	missense	probably benign	0.02
R4151:Vmn2r104	UTSW	17	20029885	missense	probably damaging	1.00
R4470:Vmn2r104	UTSW	17	20042241	missense	probably damaging	1.00
R4625:Vmn2r104	UTSW	17	20048181	missense	probably benign	0.00
R4754:Vmn2r104	UTSW	17	20040768	nonsense	probably null
R4911:Vmn2r104	UTSW	17	20030026	missense	probably benign	0.00
R5270:Vmn2r104	UTSW	17	20038266	missense	probably damaging	1.00
R5279:Vmn2r104	UTSW	17	20041884	missense	probably benign	0.07
R5311:Vmn2r104	UTSW	17	20029901	missense	probably damaging	1.00
R5370:Vmn2r104	UTSW	17	20030188	missense	probably damaging	0.97
R5461:Vmn2r104	UTSW	17	20030081	missense	probably damaging	1.00
R5683:Vmn2r104	UTSW	17	20040719	nonsense	probably null
R5795:Vmn2r104	UTSW	17	20030110	missense	probably benign	0.02
R5795:Vmn2r104	UTSW	17	20030282	missense	possibly damaging	0.89
R5970:Vmn2r104	UTSW	17	20029471	missense	probably benign	0.01
R5983:Vmn2r104	UTSW	17	20041708	missense	probably damaging	1.00
R5992:Vmn2r104	UTSW	17	20029485	missense	probably damaging	1.00
R6066:Vmn2r104	UTSW	17	20038311	missense	possibly damaging	0.69
R6156:Vmn2r104	UTSW	17	20041647	missense	probably damaging	1.00
R6182:Vmn2r104	UTSW	17	20030245	missense	probably benign	0.16
R6245:Vmn2r104	UTSW	17	20041567	missense	possibly damaging	0.69
R6333:Vmn2r104	UTSW	17	20029586	missense	probably benign	0.30
R6573:Vmn2r104	UTSW	17	20042225	missense	probably damaging	1.00
R7101:Vmn2r104	UTSW	17	20030096	missense	possibly damaging	0.65
R7123:Vmn2r104	UTSW	17	20040826	missense	probably benign	0.12
R7485:Vmn2r104	UTSW	17	20029475	missense	probably benign	0.01
R7514:Vmn2r104	UTSW	17	20029529	missense	probably damaging	1.00
R7634:Vmn2r104	UTSW	17	20041709	missense	possibly damaging	0.48
R7958:Vmn2r104	UTSW	17	20042726	missense	probably benign
R8031:Vmn2r104	UTSW	17	20042786	missense	probably benign	0.34
R8094:Vmn2r104	UTSW	17	20030221	missense	possibly damaging	0.77
R8191:Vmn2r104	UTSW	17	20030203	missense	possibly damaging	0.89
R8308:Vmn2r104	UTSW	17	20040778	missense	possibly damaging	0.55
R8691:Vmn2r104	UTSW	17	20041848	missense	probably damaging	0.98
R8795:Vmn2r104	UTSW	17	20042726	missense	probably benign
R8900:Vmn2r104	UTSW	17	20041662	missense	probably damaging	0.99
R8913:Vmn2r104	UTSW	17	20029706	missense	probably damaging	1.00
R9180:Vmn2r104	UTSW	17	20042825	missense	probably benign	0.00
R9199:Vmn2r104	UTSW	17	20041835	missense	probably damaging	0.99
R9282:Vmn2r104	UTSW	17	20040836	missense	probably damaging	1.00
R9303:Vmn2r104	UTSW	17	20048177	missense	possibly damaging	0.90
R9305:Vmn2r104	UTSW	17	20048177	missense	possibly damaging	0.90
R9322:Vmn2r104	UTSW	17	20042825	missense	probably benign	0.00
R9325:Vmn2r104	UTSW	17	20048171	missense	possibly damaging	0.95
R9414:Vmn2r104	UTSW	17	20029988	missense	probably damaging	0.99
R9785:Vmn2r104	UTSW	17	20048147	missense	probably benign
RF007:Vmn2r104	UTSW	17	20048040	missense	probably benign	0.36
Z1177:Vmn2r104	UTSW	17	20029789	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCAAGGTTGGCTCATAAGGTCCAAAAG -3'
(R):5'- GCCATTGAGGAGATCAACAGGAATCC -3'

Sequencing Primer
(F):5'- GGCTCATAAGGTCCAAAAGTAATCTG -3'
(R):5'- TCGTTGGCTTACAGCCCA -3'

Posted On

2013-10-16