|Institutional Source||Beutler Lab|
|Gene Name||ISG15 ubiquitin-like modifier|
|Essential gene?||Non essential (E-score: 0.000)|
|Stock #||R0787 (G1)|
|Chromosomal Location||156199424-156200818 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||C to T at 156199939 bp (GRCm38)|
|Amino Acid Change||Arginine to Histidine at position 44 (R44H)|
|Ref Sequence||ENSEMBL: ENSMUSP00000082548 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000085425] [ENSMUST00000105140] [ENSMUST00000180572]|
AA Change: R44H
PolyPhen 2 Score 0.195 (Sensitivity: 0.92; Specificity: 0.87)
AA Change: R44H
|Meta Mutation Damage Score||0.1055|
|Coding Region Coverage||
|Validation Efficiency||100% (56/56)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. [provided by RefSeq, Dec 2012]
PHENOTYPE: Homozygous null mice are viable and fertile and do not display immunological abnormalities. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Isg15||
(F):5'- GTCTACAGTCTGCGTCAGAAAGACC -3'
(R):5'- GTTGTGGGACCCTTTAGAACCGAG -3'
(F):5'- GTCAGAAAGACCTCATAGATGTTGC -3'
(R):5'- gtcatagcattaggagggttgag -3'