Mutagenetix > Incidental Mutation

Incidental Mutation 'R0773:Rasl10b'

76974

Institutional Source

Beutler Lab

Gene Symbol

Rasl10b

Ensembl Gene

ENSMUSG00000020684

Gene Name

RAS-like, family 10, member B

Synonyms

B230331P10Rik, VTS58635

MMRRC Submission

038953-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.146) question?

Stock #

R0773 (G1)

Quality Score

Status

Not validated

Chromosome

Chromosomal Location

83299963-83311865 bp(+) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

G to A at 83308665 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000135803 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000021022] [ENSMUST00000021022] [ENSMUST00000052521] [ENSMUST00000108140] [ENSMUST00000108140] [ENSMUST00000147301] [ENSMUST00000164425] [ENSMUST00000164425] [ENSMUST00000175848] [ENSMUST00000175848]

AlphaFold

Q5SSG5

Predicted Effect

probably null
Transcript: ENSMUST00000021022

SMART Domains

Protein: ENSMUSP00000021022
Gene: ENSMUSG00000020684

Domain	Start	End	E-Value	Type
Pfam:Miro	6	131	2.7e-16	PFAM
Pfam:Ras	6	178	1e-24	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000021022

SMART Domains

Protein: ENSMUSP00000021022
Gene: ENSMUSG00000020684

Domain	Start	End	E-Value	Type
Pfam:Miro	6	131	2.7e-16	PFAM
Pfam:Ras	6	178	1e-24	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000052521

SMART Domains

Protein: ENSMUSP00000051907
Gene: ENSMUSG00000020686

Domain	Start	End	E-Value	Type
low complexity region	4	19	N/A	INTRINSIC
CH	34	154	2.15e-15	SMART
low complexity region	167	200	N/A	INTRINSIC
Pfam:GAS2	206	274	1.2e-35	PFAM
low complexity region	444	460	N/A	INTRINSIC
low complexity region	760	775	N/A	INTRINSIC
low complexity region	817	827	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000108140

SMART Domains

Protein: ENSMUSP00000103775
Gene: ENSMUSG00000020684

Domain	Start	End	E-Value	Type
Pfam:Miro	6	131	2.7e-16	PFAM
Pfam:Ras	6	178	1e-24	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000108140

SMART Domains

Protein: ENSMUSP00000103775
Gene: ENSMUSG00000020684

Domain	Start	End	E-Value	Type
Pfam:Miro	6	131	2.7e-16	PFAM
Pfam:Ras	6	178	1e-24	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000147301

SMART Domains

Protein: ENSMUSP00000119454
Gene: ENSMUSG00000020686

Domain	Start	End	E-Value	Type
low complexity region	4	19	N/A	INTRINSIC
CH	34	154	2.15e-15	SMART
low complexity region	167	185	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000164425

SMART Domains

Protein: ENSMUSP00000132696
Gene: ENSMUSG00000020684

Domain	Start	End	E-Value	Type
Pfam:Ras	6	178	6.6e-25	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000164425

SMART Domains

Protein: ENSMUSP00000132696
Gene: ENSMUSG00000020684

Domain	Start	End	E-Value	Type
Pfam:Ras	6	178	6.6e-25	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000175848

SMART Domains

Protein: ENSMUSP00000135803
Gene: ENSMUSG00000020684

Domain	Start	End	E-Value	Type
Pfam:Ras	6	120	2.3e-12	PFAM
Pfam:Miro	6	123	8.2e-13	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000175848

SMART Domains

Protein: ENSMUSP00000135803
Gene: ENSMUSG00000020684

Domain	Start	End	E-Value	Type
Pfam:Ras	6	120	2.3e-12	PFAM
Pfam:Miro	6	123	8.2e-13	PFAM

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 95.2%
20x: 87.2%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele have smaller than normal large dense-core vesicles in atrial myocytes and exhibit impaired atrial natriuretic peptide secretion. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 1 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Prl3b1	G	A	13: 27,427,848 (GRCm39)	A53T	probably benign	Het

Other mutations in Rasl10b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02003:Rasl10b	APN	11	83,308,679 (GRCm39)	missense	probably damaging	1.00
R0378:Rasl10b	UTSW	11	83,309,519 (GRCm39)	missense	probably damaging	1.00
R0772:Rasl10b	UTSW	11	83,308,665 (GRCm39)	splice site	probably null
R0829:Rasl10b	UTSW	11	83,308,665 (GRCm39)	splice site	probably null
R0830:Rasl10b	UTSW	11	83,308,665 (GRCm39)	splice site	probably null
R0831:Rasl10b	UTSW	11	83,308,665 (GRCm39)	splice site	probably null
R1363:Rasl10b	UTSW	11	83,308,665 (GRCm39)	splice site	probably null
R1364:Rasl10b	UTSW	11	83,308,665 (GRCm39)	splice site	probably null
R1365:Rasl10b	UTSW	11	83,308,665 (GRCm39)	splice site	probably null
R1366:Rasl10b	UTSW	11	83,308,665 (GRCm39)	splice site	probably null
R1367:Rasl10b	UTSW	11	83,308,665 (GRCm39)	splice site	probably null
R1368:Rasl10b	UTSW	11	83,308,665 (GRCm39)	splice site	probably null
R1389:Rasl10b	UTSW	11	83,308,665 (GRCm39)	splice site	probably null
R3771:Rasl10b	UTSW	11	83,309,349 (GRCm39)	missense	probably benign	0.04
R4925:Rasl10b	UTSW	11	83,303,505 (GRCm39)	missense	probably damaging	1.00
R5625:Rasl10b	UTSW	11	83,309,640 (GRCm39)	missense	probably damaging	1.00
R8830:Rasl10b	UTSW	11	83,303,502 (GRCm39)	missense	probably damaging	1.00
R9526:Rasl10b	UTSW	11	83,303,590 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCCTCACAAAATAGTGTGGTGGGC -3'
(R):5'- TGTACCCCTCTTTTGGAGACAGGC -3'

Sequencing Primer
(F):5'- CCAGGAAGTAACCAGCTTGTG -3'
(R):5'- TTTTGGAGACAGGCCAGCATC -3'

Posted On

2013-10-16