Incidental Mutation 'R0849:Cand2'
ID 77061
Institutional Source Beutler Lab
Gene Symbol Cand2
Ensembl Gene ENSMUSG00000030319
Gene Name cullin associated and neddylation dissociated 2 (putative)
Synonyms Tp120b, 2210404G23Rik
MMRRC Submission 039028-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R0849 (G1)
Quality Score 120
Status Not validated
Chromosome 6
Chromosomal Location 115751518-115782516 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to G at 115769352 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Threonine to Alanine at position 721 (T721A)
Ref Sequence ENSEMBL: ENSMUSP00000075377 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000075995]
AlphaFold Q6ZQ73
Predicted Effect probably damaging
Transcript: ENSMUST00000075995
AA Change: T721A

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)
SMART Domains Protein: ENSMUSP00000075377
Gene: ENSMUSG00000030319
AA Change: T721A

DomainStartEndE-ValueType
low complexity region 325 347 N/A INTRINSIC
low complexity region 536 548 N/A INTRINSIC
low complexity region 553 562 N/A INTRINSIC
low complexity region 665 686 N/A INTRINSIC
low complexity region 736 748 N/A INTRINSIC
Pfam:HEAT 861 890 4.4e-5 PFAM
Pfam:TIP120 1044 1209 6e-64 PFAM
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.5%
  • 10x: 96.5%
  • 20x: 92.5%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 43 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Arhgap5 T C 12: 52,566,406 (GRCm39) F1126L probably benign Het
Arhgef11 G T 3: 87,643,203 (GRCm39) A1472S probably benign Het
Armc9 G A 1: 86,184,992 (GRCm39) R681K probably benign Het
Atxn2 T A 5: 121,885,484 (GRCm39) probably null Het
B3gnt6 C A 7: 97,843,950 (GRCm39) L3F probably benign Het
Cntn2 A T 1: 132,450,124 (GRCm39) M590K probably benign Het
Cntn4 A T 6: 106,644,418 (GRCm39) D622V probably damaging Het
Des T G 1: 75,337,272 (GRCm39) S71A probably benign Het
Dnah5 C A 15: 28,263,745 (GRCm39) R827S probably damaging Het
Dstn G A 2: 143,780,455 (GRCm39) G52S probably benign Het
Eln T A 5: 134,736,835 (GRCm39) R704* probably null Het
Fezf2 T A 14: 12,342,607 (GRCm38) K419N probably damaging Het
Gm2381 G A 7: 42,469,372 (GRCm39) Q251* probably null Het
Grin3a G A 4: 49,665,501 (GRCm39) R1045* probably null Het
Herc2 T A 7: 55,856,326 (GRCm39) H3921Q probably damaging Het
Hs3st2 A G 7: 121,100,255 (GRCm39) E367G possibly damaging Het
Hydin G A 8: 111,325,616 (GRCm39) R4675H probably damaging Het
Inhca A C 9: 103,140,256 (GRCm39) Y488D possibly damaging Het
Irf2bp1 T C 7: 18,738,659 (GRCm39) S100P possibly damaging Het
Itga10 A G 3: 96,559,846 (GRCm39) I500M possibly damaging Het
Kcnt2 T C 1: 140,435,500 (GRCm39) V496A probably damaging Het
Mansc1 T C 6: 134,587,670 (GRCm39) D169G probably benign Het
Mdn1 T A 4: 32,741,835 (GRCm39) S3869T probably damaging Het
Mrgprb4 G A 7: 47,848,868 (GRCm39) T20I probably benign Het
Nek4 A G 14: 30,679,253 (GRCm39) E198G probably damaging Het
Or12e8 G T 2: 87,188,609 (GRCm39) V274L probably benign Het
Or2y10 G A 11: 49,455,129 (GRCm39) C127Y probably damaging Het
Or5w11 C A 2: 87,459,626 (GRCm39) T273K possibly damaging Het
Ppfia4 A T 1: 134,247,110 (GRCm39) F537I probably benign Het
Rarb A G 14: 16,434,293 (GRCm38) V295A probably damaging Het
Ryr1 A G 7: 28,740,104 (GRCm39) S3941P probably damaging Het
Scnn1b A G 7: 121,511,698 (GRCm39) N370S probably damaging Het
Stard9 C A 2: 120,504,117 (GRCm39) S221R probably damaging Het
Sv2c C T 13: 96,126,319 (GRCm39) G311D probably damaging Het
Tas2r109 T C 6: 132,957,856 (GRCm39) I25V probably benign Het
Tbcel A C 9: 42,348,453 (GRCm39) I305S probably damaging Het
Tmem35b A G 4: 127,019,990 (GRCm39) probably null Het
Tmem38b T C 4: 53,840,765 (GRCm39) L60S probably damaging Het
Tmtc4 A G 14: 123,182,966 (GRCm39) I244T possibly damaging Het
Usp9y C A Y: 1,394,002 (GRCm39) C576F probably damaging Het
Vcan A T 13: 89,853,072 (GRCm39) N629K possibly damaging Het
Vmn2r22 T C 6: 123,614,363 (GRCm39) Y409C probably damaging Het
Vmn2r-ps158 A G 7: 42,674,142 (GRCm39) Y400C probably damaging Het
Other mutations in Cand2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01323:Cand2 APN 6 115,762,086 (GRCm39) missense probably benign
IGL01329:Cand2 APN 6 115,759,755 (GRCm39) missense probably benign 0.43
IGL01777:Cand2 APN 6 115,769,818 (GRCm39) missense probably damaging 0.99
IGL02008:Cand2 APN 6 115,780,599 (GRCm39) missense probably damaging 1.00
IGL02185:Cand2 APN 6 115,766,471 (GRCm39) missense probably benign 0.01
IGL02219:Cand2 APN 6 115,780,773 (GRCm39) missense probably damaging 1.00
IGL02240:Cand2 APN 6 115,780,623 (GRCm39) missense probably damaging 1.00
IGL02329:Cand2 APN 6 115,766,568 (GRCm39) missense probably damaging 1.00
IGL02396:Cand2 APN 6 115,768,149 (GRCm39) splice site probably benign
IGL02893:Cand2 APN 6 115,768,921 (GRCm39) missense probably damaging 1.00
IGL03161:Cand2 APN 6 115,769,698 (GRCm39) missense probably benign 0.45
IGL03170:Cand2 APN 6 115,774,861 (GRCm39) missense probably damaging 1.00
IGL03257:Cand2 APN 6 115,776,944 (GRCm39) missense possibly damaging 0.80
succor UTSW 6 115,768,153 (GRCm39) missense probably damaging 1.00
R0196:Cand2 UTSW 6 115,766,463 (GRCm39) missense probably damaging 1.00
R0390:Cand2 UTSW 6 115,751,614 (GRCm39) missense possibly damaging 0.90
R0534:Cand2 UTSW 6 115,764,197 (GRCm39) missense probably damaging 0.96
R0630:Cand2 UTSW 6 115,780,766 (GRCm39) missense probably damaging 1.00
R0631:Cand2 UTSW 6 115,780,766 (GRCm39) missense probably damaging 1.00
R0662:Cand2 UTSW 6 115,764,171 (GRCm39) missense probably benign 0.00
R0671:Cand2 UTSW 6 115,780,766 (GRCm39) missense probably damaging 1.00
R0708:Cand2 UTSW 6 115,780,766 (GRCm39) missense probably damaging 1.00
R1992:Cand2 UTSW 6 115,762,093 (GRCm39) missense possibly damaging 0.88
R3428:Cand2 UTSW 6 115,766,668 (GRCm39) missense probably benign
R3773:Cand2 UTSW 6 115,762,178 (GRCm39) missense probably damaging 0.96
R4329:Cand2 UTSW 6 115,776,949 (GRCm39) missense possibly damaging 0.64
R4489:Cand2 UTSW 6 115,766,427 (GRCm39) missense probably damaging 1.00
R4553:Cand2 UTSW 6 115,769,172 (GRCm39) missense probably damaging 1.00
R4577:Cand2 UTSW 6 115,768,220 (GRCm39) missense probably damaging 1.00
R4634:Cand2 UTSW 6 115,774,948 (GRCm39) missense probably damaging 1.00
R4850:Cand2 UTSW 6 115,778,909 (GRCm39) missense probably benign 0.14
R5155:Cand2 UTSW 6 115,769,219 (GRCm39) missense probably benign 0.42
R5190:Cand2 UTSW 6 115,766,474 (GRCm39) missense probably damaging 1.00
R5378:Cand2 UTSW 6 115,778,912 (GRCm39) missense probably benign 0.00
R5407:Cand2 UTSW 6 115,762,161 (GRCm39) missense possibly damaging 0.76
R5698:Cand2 UTSW 6 115,768,704 (GRCm39) missense probably damaging 1.00
R5701:Cand2 UTSW 6 115,774,893 (GRCm39) missense probably damaging 0.99
R6172:Cand2 UTSW 6 115,768,271 (GRCm39) missense probably benign 0.00
R6763:Cand2 UTSW 6 115,776,930 (GRCm39) missense probably benign 0.00
R6920:Cand2 UTSW 6 115,768,250 (GRCm39) missense possibly damaging 0.93
R7229:Cand2 UTSW 6 115,768,153 (GRCm39) missense probably damaging 1.00
R7520:Cand2 UTSW 6 115,762,212 (GRCm39) nonsense probably null
R8183:Cand2 UTSW 6 115,768,879 (GRCm39) missense probably benign 0.14
R8698:Cand2 UTSW 6 115,763,852 (GRCm39) missense probably damaging 1.00
R8755:Cand2 UTSW 6 115,769,941 (GRCm39) missense probably damaging 1.00
R8795:Cand2 UTSW 6 115,763,889 (GRCm39) missense probably benign 0.01
R8900:Cand2 UTSW 6 115,757,894 (GRCm39) missense probably benign 0.00
R9072:Cand2 UTSW 6 115,769,490 (GRCm39) missense probably damaging 0.99
R9242:Cand2 UTSW 6 115,768,923 (GRCm39) missense probably benign 0.27
R9262:Cand2 UTSW 6 115,759,730 (GRCm39) missense probably benign 0.27
R9547:Cand2 UTSW 6 115,759,757 (GRCm39) missense probably benign 0.00
R9676:Cand2 UTSW 6 115,769,122 (GRCm39) missense probably benign 0.26
Predicted Primers PCR Primer
(F):5'- ATCGACTTGGGGACGACCTAGAAC -3'
(R):5'- TGCAGTGAGCAGGCTAATGAGC -3'

Sequencing Primer
(F):5'- AATGAGATCACCCGGTTGC -3'
(R):5'- TAATGAGCTCCGAGTACTCCAC -3'
Posted On 2013-10-16