Mutagenetix > Incidental Mutation

Incidental Mutation 'R0833:Otog'

77639

Institutional Source

Beutler Lab

Gene Symbol

Otog

Ensembl Gene

ENSMUSG00000009487

Gene Name

otogelin

Synonyms

Otgn

MMRRC Submission

039012-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.844) question?

Stock #

R0833 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

46240987-46311434 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 46269362 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Isoleucine at position 954 (T954I)

Ref Sequence

ENSEMBL: ENSMUSP00000130949 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164538]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000164538
AA Change: T954I

PolyPhen 2 Score 0.937 (Sensitivity: 0.80; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000130949
Gene: ENSMUSG00000009487
AA Change: T954I

Domain	Start	End	E-Value	Type
signal peptide	1	25	N/A	INTRINSIC
low complexity region	72	85	N/A	INTRINSIC
VWD	128	288	7.98e-45	SMART
C8	330	404	1.05e-13	SMART
VWC	463	505	1.24e0	SMART
VWD	490	655	4.94e-50	SMART
C8	693	758	1.23e-5	SMART
Pfam:TIL	767	831	3.4e-13	PFAM
VWC	935	983	1.83e0	SMART
VWD	962	1118	6.05e-45	SMART
C8	1153	1227	1.02e-34	SMART
Pfam:AbfB	1270	1384	7.5e-10	PFAM
low complexity region	1488	1513	N/A	INTRINSIC
low complexity region	1524	1536	N/A	INTRINSIC
low complexity region	1560	1578	N/A	INTRINSIC
low complexity region	1637	1644	N/A	INTRINSIC
low complexity region	1677	1696	N/A	INTRINSIC
low complexity region	1731	1748	N/A	INTRINSIC
VWD	2087	2251	2.37e-29	SMART
C8	2287	2356	4.93e-19	SMART
low complexity region	2443	2449	N/A	INTRINSIC
CT	2828	2911	3.46e-28	SMART

Meta Mutation Damage Score

0.1019

Coding Region Coverage

1x: 99.4%
3x: 98.8%
10x: 97.3%
20x: 94.6%

Validation Efficiency

100% (97/97)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a component of the acellular membranes of the inner ear. Disruption of the orthologous mouse gene shows that it plays a role in auditory and vestibular functions. It is involved in fibrillar network organization, the anchoring of otoconial membranes and cupulae to the neuroepithelia, and likely in sound stimulation resistance. Mutations in this gene cause autosomal recessive nonsyndromic deafness, type 18B. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2014]
PHENOTYPE: Homozygotes for a number of different spontaneous and targeted mutations exhibit vestibular dysfunction, including circling, head tilt, impaired balance, coordination, and placing response. Mutants have impaired hearing, decreased brain stem auditory evoked potential, and ear abnormalities. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 93 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700113H08Rik	T	C	10: 87,165,069 (GRCm38)	L41P	probably damaging	Het
3110082I17Rik	A	G	5: 139,364,120 (GRCm38)	V58A	possibly damaging	Het
4932415M13Rik	A	T	17: 53,724,346 (GRCm38)		noncoding transcript	Het
Aldh1a7	C	T	19: 20,702,243 (GRCm38)	V390M	probably damaging	Het
Alg14	A	G	3: 121,298,610 (GRCm38)	H34R	probably damaging	Het
Ankrd27	A	G	7: 35,608,347 (GRCm38)	N337S	probably damaging	Het
Apoa2	T	A	1: 171,225,379 (GRCm38)		probably benign	Het
Arl2	T	G	19: 6,136,022 (GRCm38)	K126T	probably damaging	Het
Ascc3	T	C	10: 50,845,666 (GRCm38)	W2072R	probably benign	Het
Astl	T	C	2: 127,342,419 (GRCm38)	F21L	probably benign	Het
Asxl3	A	G	18: 22,516,040 (GRCm38)	D362G	probably damaging	Het
Bdp1	A	T	13: 100,035,825 (GRCm38)	H2094Q	probably benign	Het
Cacna2d4	C	T	6: 119,307,286 (GRCm38)	R745W	probably damaging	Het
Ccdc85a	T	A	11: 28,583,296 (GRCm38)	I83F	probably damaging	Het
Ccnt2	T	A	1: 127,802,394 (GRCm38)	M336K	probably benign	Het
Cd226	A	C	18: 89,207,020 (GRCm38)		probably benign	Het
Ceacam23	C	A	7: 17,904,981 (GRCm38)	A301E	possibly damaging	Het
Ces1f	A	T	8: 93,270,024 (GRCm38)	S214T	probably damaging	Het
Clip1	T	C	5: 123,630,721 (GRCm38)	D605G	probably benign	Het
Col26a1	A	G	5: 136,765,300 (GRCm38)		probably null	Het
Crtc1	A	G	8: 70,393,013 (GRCm38)	V306A	probably benign	Het
D130043K22Rik	G	A	13: 24,863,580 (GRCm38)		probably benign	Het
D17H6S53E	A	T	17: 35,127,409 (GRCm38)		probably null	Het
Dnah11	G	A	12: 118,196,662 (GRCm38)	A111V	probably benign	Het
Dyrk2	T	C	10: 118,861,122 (GRCm38)	H77R	probably benign	Het
E330017A01Rik	G	A	16: 58,635,523 (GRCm38)	S129L	probably damaging	Het
Epha3	A	G	16: 63,603,519 (GRCm38)		probably benign	Het
Epn2	T	C	11: 61,519,491 (GRCm38)	N611S	probably benign	Het
Erich6	A	C	3: 58,618,944 (GRCm38)		probably benign	Het
Fam217b	T	C	2: 178,420,989 (GRCm38)	S249P	probably benign	Het
Fam219b	A	G	9: 57,538,016 (GRCm38)		probably benign	Het
Fryl	A	T	5: 73,089,081 (GRCm38)		probably benign	Het
Gm14496	T	C	2: 181,996,266 (GRCm38)	W378R	probably damaging	Het
Gm57858	A	G	3: 36,020,213 (GRCm38)		probably benign	Het
Gm8674	A	T	13: 49,904,575 (GRCm38)		noncoding transcript	Het
Grap	T	A	11: 61,660,239 (GRCm38)	D32E	possibly damaging	Het
Grk2	C	A	19: 4,289,357 (GRCm38)	L428F	probably damaging	Het
Grm8	T	A	6: 27,363,179 (GRCm38)	E779V	probably damaging	Het
Hipk1	A	G	3: 103,754,296 (GRCm38)	S670P	probably damaging	Het
Ilrun	A	T	17: 27,786,138 (GRCm38)	S148R	probably damaging	Het
Itgae	A	T	11: 73,129,206 (GRCm38)	M845L	probably benign	Het
Itih1	A	T	14: 30,941,555 (GRCm38)	V164E	probably damaging	Het
Itpka	T	A	2: 119,750,831 (GRCm38)	N448K	probably damaging	Het
Jak3	A	C	8: 71,683,978 (GRCm38)	N643T	probably damaging	Het
Lrfn5	A	C	12: 61,839,668 (GRCm38)	T81P	probably damaging	Het
Lrrc45	T	A	11: 120,718,193 (GRCm38)		probably null	Het
Marchf6	T	C	15: 31,480,291 (GRCm38)	Y562C	probably benign	Het
Mcrs1	T	C	15: 99,243,449 (GRCm38)		probably benign	Het
Meis1	G	A	11: 18,881,767 (GRCm38)	H424Y	possibly damaging	Het
Mst1r	G	A	9: 107,913,167 (GRCm38)	V660I	probably benign	Het
Mst1r	A	G	9: 107,914,776 (GRCm38)	N837S	probably benign	Het
Mthfd2l	T	C	5: 90,946,942 (GRCm38)	V90A	probably damaging	Het
Mtnr1a	A	T	8: 45,087,937 (GRCm38)	I312F	probably benign	Het
Or10g3b	T	C	14: 52,349,378 (GRCm38)	I223V	probably benign	Het
Or51g1	C	A	7: 102,984,836 (GRCm38)	L109F	possibly damaging	Het
Or8b12b	T	C	9: 37,773,076 (GRCm38)	V139A	probably benign	Het
Pdcd6	A	G	13: 74,316,324 (GRCm38)		probably benign	Het
Phlpp2	A	G	8: 109,937,106 (GRCm38)	T926A	probably damaging	Het
Plec	GGCAGCAG	GGCAGCAGCAG	15: 76,181,907 (GRCm38)		probably benign	Het
Plekha5	T	A	6: 140,589,634 (GRCm38)		probably benign	Het
Ppp1r16a	C	T	15: 76,693,669 (GRCm38)	Q328*	probably null	Het
Pramel18	G	A	4: 101,910,097 (GRCm38)	C181Y	probably damaging	Het
Pramel23	A	T	4: 143,698,486 (GRCm38)	M129K	probably benign	Het
Rab27b	T	C	18: 69,987,041 (GRCm38)		probably benign	Het
Rapsn	T	C	2: 91,036,808 (GRCm38)	Y152H	probably damaging	Het
Rasd1	A	T	11: 59,964,553 (GRCm38)	F85I	probably damaging	Het
Rgs1	A	T	1: 144,247,933 (GRCm38)	S85T	probably damaging	Het
Samd9l	T	C	6: 3,372,725 (GRCm38)	E1512G	possibly damaging	Het
Sgsm1	C	T	5: 113,279,184 (GRCm38)	A127T	probably benign	Het
Shc1	A	G	3: 89,422,969 (GRCm38)	D70G	probably damaging	Het
Slc26a1	T	A	5: 108,673,523 (GRCm38)	T167S	probably benign	Het
Slc26a7	A	T	4: 14,593,873 (GRCm38)	Y81N	probably damaging	Het
Slc2a12	T	C	10: 22,702,016 (GRCm38)		probably benign	Het
Slc30a7	T	A	3: 115,990,140 (GRCm38)		probably null	Het
Slc44a5	T	C	3: 154,265,474 (GRCm38)	S654P	probably damaging	Het
Stard9	T	G	2: 120,696,999 (GRCm38)	S1246A	possibly damaging	Het
Stxbp5	A	T	10: 9,865,099 (GRCm38)	S116R	probably damaging	Het
Syvn1	C	T	19: 6,052,453 (GRCm38)	P517L	probably benign	Het
Tas2r105	T	C	6: 131,687,430 (GRCm38)	I12V	probably benign	Het
Tas2r121	A	G	6: 132,700,362 (GRCm38)	S216P	probably damaging	Het
Tcof1	T	C	18: 60,845,832 (GRCm38)	D48G	probably damaging	Het
Tgm3	C	T	2: 130,026,682 (GRCm38)		probably benign	Het
Tle2	T	C	10: 81,588,947 (GRCm38)	F667L	probably damaging	Het
Tnfaip3	C	A	10: 19,002,949 (GRCm38)	A704S	probably benign	Het
Trabd2b	A	G	4: 114,580,322 (GRCm38)	Q232R	probably benign	Het
Ttc28	T	A	5: 111,231,081 (GRCm38)	I1144N	probably damaging	Het
Ucp3	T	A	7: 100,479,541 (GRCm38)	C25*	probably null	Het
Ugt3a2	A	G	15: 9,370,150 (GRCm38)	D460G	probably damaging	Het
Urb1	T	C	16: 90,795,448 (GRCm38)	D308G	possibly damaging	Het
Ush1g	G	T	11: 115,318,868 (GRCm38)	R167S	possibly damaging	Het
Vav3	A	G	3: 109,647,679 (GRCm38)	N81S	possibly damaging	Het
Vmn2r108	A	T	17: 20,471,459 (GRCm38)	D267E	probably benign	Het
Zng1	A	G	19: 24,940,839 (GRCm38)		probably benign	Het

Other mutations in Otog

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00516:Otog	APN	7	46,251,282 (GRCm38)	missense	probably damaging	1.00
IGL00725:Otog	APN	7	46,274,092 (GRCm38)	missense	probably damaging	1.00
IGL00757:Otog	APN	7	46,290,128 (GRCm38)	missense	probably damaging	1.00
IGL00822:Otog	APN	7	46,295,880 (GRCm38)	missense	probably benign	0.24
IGL01354:Otog	APN	7	46,289,726 (GRCm38)	missense	probably damaging	1.00
IGL01567:Otog	APN	7	46,276,615 (GRCm38)	splice site	probably benign
IGL02034:Otog	APN	7	46,295,993 (GRCm38)	nonsense	probably null
IGL02090:Otog	APN	7	46,300,147 (GRCm38)	missense	probably damaging	1.00
IGL02132:Otog	APN	7	46,305,479 (GRCm38)	missense	probably damaging	0.99
IGL02148:Otog	APN	7	46,300,587 (GRCm38)	missense	probably damaging	1.00
IGL02173:Otog	APN	7	46,276,741 (GRCm38)	splice site	probably benign
IGL02199:Otog	APN	7	46,277,351 (GRCm38)	missense	possibly damaging	0.90
IGL02216:Otog	APN	7	46,301,468 (GRCm38)	missense	probably damaging	1.00
IGL02322:Otog	APN	7	46,301,457 (GRCm38)	missense	probably benign	0.01
IGL02330:Otog	APN	7	46,288,069 (GRCm38)	missense	possibly damaging	0.84
IGL02529:Otog	APN	7	46,259,957 (GRCm38)	missense	probably damaging	0.99
IGL02898:Otog	APN	7	46,310,138 (GRCm38)	missense	probably damaging	1.00
IGL02970:Otog	APN	7	46,295,867 (GRCm38)	missense	probably benign	0.11
IGL03085:Otog	APN	7	46,305,922 (GRCm38)	critical splice donor site	probably null
IGL03108:Otog	APN	7	46,251,338 (GRCm38)	missense	probably damaging	1.00
IGL03275:Otog	APN	7	46,306,230 (GRCm38)	missense	probably damaging	1.00
R0282_Otog_616	UTSW	7	46,277,493 (GRCm38)	missense	possibly damaging	0.93
R0636_otog_678	UTSW	7	46,264,228 (GRCm38)	critical splice donor site	probably null
R1029_otog_141	UTSW	7	46,274,595 (GRCm38)	missense	probably damaging	1.00
BB010:Otog	UTSW	7	46,310,147 (GRCm38)	missense	probably damaging	1.00
BB020:Otog	UTSW	7	46,310,147 (GRCm38)	missense	probably damaging	1.00
I1329:Otog	UTSW	7	46,246,503 (GRCm38)	missense	probably benign	0.02
IGL02984:Otog	UTSW	7	46,305,508 (GRCm38)	missense	probably damaging	0.98
PIT4472001:Otog	UTSW	7	46,295,849 (GRCm38)	missense	probably damaging	1.00
R0032:Otog	UTSW	7	46,304,231 (GRCm38)	missense	probably damaging	0.97
R0032:Otog	UTSW	7	46,288,213 (GRCm38)	nonsense	probably null
R0105:Otog	UTSW	7	46,288,366 (GRCm38)	missense	possibly damaging	0.79
R0164:Otog	UTSW	7	46,304,231 (GRCm38)	missense	probably damaging	0.97
R0164:Otog	UTSW	7	46,304,231 (GRCm38)	missense	probably damaging	0.97
R0165:Otog	UTSW	7	46,304,231 (GRCm38)	missense	probably damaging	0.97
R0166:Otog	UTSW	7	46,304,231 (GRCm38)	missense	probably damaging	0.97
R0167:Otog	UTSW	7	46,304,231 (GRCm38)	missense	probably damaging	0.97
R0240:Otog	UTSW	7	46,264,032 (GRCm38)	splice site	probably null
R0240:Otog	UTSW	7	46,264,032 (GRCm38)	splice site	probably null
R0242:Otog	UTSW	7	46,267,381 (GRCm38)	missense	probably damaging	0.98
R0242:Otog	UTSW	7	46,267,381 (GRCm38)	missense	probably damaging	0.98
R0282:Otog	UTSW	7	46,277,493 (GRCm38)	missense	possibly damaging	0.93
R0392:Otog	UTSW	7	46,250,075 (GRCm38)	missense	probably benign	0.00
R0436:Otog	UTSW	7	46,265,936 (GRCm38)	splice site	probably benign
R0441:Otog	UTSW	7	46,305,877 (GRCm38)	missense	probably damaging	1.00
R0499:Otog	UTSW	7	46,273,832 (GRCm38)	missense	probably damaging	1.00
R0530:Otog	UTSW	7	46,298,244 (GRCm38)	missense	probably damaging	0.98
R0541:Otog	UTSW	7	46,269,249 (GRCm38)	splice site	probably benign
R0600:Otog	UTSW	7	46,251,395 (GRCm38)	splice site	probably benign
R0626:Otog	UTSW	7	46,271,373 (GRCm38)	missense	possibly damaging	0.95
R0636:Otog	UTSW	7	46,264,228 (GRCm38)	critical splice donor site	probably null
R0764:Otog	UTSW	7	46,300,494 (GRCm38)	missense	probably benign	0.00
R0836:Otog	UTSW	7	46,269,362 (GRCm38)	missense	possibly damaging	0.94
R0844:Otog	UTSW	7	46,287,828 (GRCm38)	missense	possibly damaging	0.53
R1029:Otog	UTSW	7	46,274,595 (GRCm38)	missense	probably damaging	1.00
R1116:Otog	UTSW	7	46,300,601 (GRCm38)	splice site	probably benign
R1134:Otog	UTSW	7	46,298,514 (GRCm38)	missense	probably damaging	1.00
R1183:Otog	UTSW	7	46,289,755 (GRCm38)	missense	probably benign	0.41
R1204:Otog	UTSW	7	46,259,911 (GRCm38)	missense	probably benign	0.16
R1301:Otog	UTSW	7	46,289,689 (GRCm38)	missense	probably damaging	1.00
R1344:Otog	UTSW	7	46,274,615 (GRCm38)	missense	probably damaging	1.00
R1384:Otog	UTSW	7	46,273,695 (GRCm38)	splice site	probably benign
R1418:Otog	UTSW	7	46,274,615 (GRCm38)	missense	probably damaging	1.00
R1432:Otog	UTSW	7	46,300,583 (GRCm38)	missense	probably damaging	1.00
R1479:Otog	UTSW	7	46,295,978 (GRCm38)	missense	possibly damaging	0.75
R1521:Otog	UTSW	7	46,259,264 (GRCm38)	missense	possibly damaging	0.71
R1589:Otog	UTSW	7	46,283,908 (GRCm38)	missense	probably benign	0.18
R1671:Otog	UTSW	7	46,261,786 (GRCm38)	missense	probably damaging	1.00
R1773:Otog	UTSW	7	46,288,159 (GRCm38)	missense	probably benign	0.28
R1806:Otog	UTSW	7	46,290,937 (GRCm38)	critical splice acceptor site	probably null
R1843:Otog	UTSW	7	46,246,283 (GRCm38)	missense	probably damaging	1.00
R1873:Otog	UTSW	7	46,269,343 (GRCm38)	missense	probably damaging	1.00
R1923:Otog	UTSW	7	46,246,283 (GRCm38)	missense	probably damaging	1.00
R1927:Otog	UTSW	7	46,246,283 (GRCm38)	missense	probably damaging	1.00
R2008:Otog	UTSW	7	46,264,074 (GRCm38)	missense	probably benign	0.43
R2048:Otog	UTSW	7	46,287,639 (GRCm38)	missense	probably damaging	1.00
R2131:Otog	UTSW	7	46,250,100 (GRCm38)	missense	probably damaging	1.00
R2153:Otog	UTSW	7	46,302,904 (GRCm38)	missense	probably damaging	1.00
R2240:Otog	UTSW	7	46,241,029 (GRCm38)	start codon destroyed	probably null
R2278:Otog	UTSW	7	46,300,044 (GRCm38)	missense	probably damaging	1.00
R2407:Otog	UTSW	7	46,241,540 (GRCm38)	missense	probably benign	0.10
R2424:Otog	UTSW	7	46,298,169 (GRCm38)	nonsense	probably null
R2513:Otog	UTSW	7	46,305,590 (GRCm38)	critical splice donor site	probably null
R2863:Otog	UTSW	7	46,269,306 (GRCm38)	missense	probably damaging	1.00
R3148:Otog	UTSW	7	46,290,169 (GRCm38)	missense	probably damaging	1.00
R3732:Otog	UTSW	7	46,288,368 (GRCm38)	missense	probably benign	0.03
R3732:Otog	UTSW	7	46,288,368 (GRCm38)	missense	probably benign	0.03
R3733:Otog	UTSW	7	46,288,368 (GRCm38)	missense	probably benign	0.03
R3734:Otog	UTSW	7	46,288,368 (GRCm38)	missense	probably benign	0.03
R3855:Otog	UTSW	7	46,273,760 (GRCm38)	missense	possibly damaging	0.65
R3880:Otog	UTSW	7	46,288,021 (GRCm38)	missense	possibly damaging	0.93
R4081:Otog	UTSW	7	46,288,299 (GRCm38)	missense	possibly damaging	0.92
R4349:Otog	UTSW	7	46,274,189 (GRCm38)	missense	probably damaging	0.99
R4382:Otog	UTSW	7	46,289,698 (GRCm38)	missense	probably damaging	1.00
R4392:Otog	UTSW	7	46,285,124 (GRCm38)	missense	probably damaging	0.98
R4520:Otog	UTSW	7	46,241,053 (GRCm38)	unclassified	probably benign
R4569:Otog	UTSW	7	46,310,147 (GRCm38)	missense	probably damaging	1.00
R4580:Otog	UTSW	7	46,287,801 (GRCm38)	missense	possibly damaging	0.78
R4672:Otog	UTSW	7	46,289,786 (GRCm38)	missense	probably damaging	0.98
R4764:Otog	UTSW	7	46,288,519 (GRCm38)	missense	probably benign	0.29
R4910:Otog	UTSW	7	46,298,534 (GRCm38)	missense	probably damaging	1.00
R4910:Otog	UTSW	7	46,264,062 (GRCm38)	missense	probably damaging	1.00
R4913:Otog	UTSW	7	46,264,102 (GRCm38)	missense	probably benign	0.31
R4975:Otog	UTSW	7	46,287,991 (GRCm38)	missense	probably benign	0.00
R4996:Otog	UTSW	7	46,305,510 (GRCm38)	nonsense	probably null
R4996:Otog	UTSW	7	46,298,606 (GRCm38)	missense	possibly damaging	0.51
R5116:Otog	UTSW	7	46,273,767 (GRCm38)	missense	probably benign	0.34
R5138:Otog	UTSW	7	46,250,006 (GRCm38)	missense	possibly damaging	0.61
R5169:Otog	UTSW	7	46,298,148 (GRCm38)	missense	probably benign	0.06
R5239:Otog	UTSW	7	46,287,435 (GRCm38)	missense	probably benign	0.15
R5277:Otog	UTSW	7	46,246,621 (GRCm38)	missense	possibly damaging	0.89
R5287:Otog	UTSW	7	46,269,329 (GRCm38)	missense	probably damaging	0.98
R5299:Otog	UTSW	7	46,288,851 (GRCm38)	missense	probably benign	0.16
R5378:Otog	UTSW	7	46,255,004 (GRCm38)	missense	probably damaging	1.00
R5382:Otog	UTSW	7	46,249,004 (GRCm38)	missense	probably damaging	1.00
R5487:Otog	UTSW	7	46,288,768 (GRCm38)	missense	probably benign	0.27
R5507:Otog	UTSW	7	46,261,699 (GRCm38)	missense	probably damaging	1.00
R5517:Otog	UTSW	7	46,274,571 (GRCm38)	missense	probably damaging	1.00
R5643:Otog	UTSW	7	46,287,447 (GRCm38)	missense	probably damaging	1.00
R5757:Otog	UTSW	7	46,241,121 (GRCm38)	critical splice donor site	probably null
R5910:Otog	UTSW	7	46,298,598 (GRCm38)	missense	possibly damaging	0.94
R6019:Otog	UTSW	7	46,288,950 (GRCm38)	missense	probably benign	0.00
R6150:Otog	UTSW	7	46,264,059 (GRCm38)	missense	possibly damaging	0.82
R6225:Otog	UTSW	7	46,249,034 (GRCm38)	missense	possibly damaging	0.67
R6271:Otog	UTSW	7	46,252,040 (GRCm38)	missense	probably damaging	1.00
R6317:Otog	UTSW	7	46,301,215 (GRCm38)	missense	probably damaging	1.00
R6454:Otog	UTSW	7	46,305,817 (GRCm38)	missense	probably damaging	1.00
R6640:Otog	UTSW	7	46,261,743 (GRCm38)	missense	possibly damaging	0.92
R6753:Otog	UTSW	7	46,249,071 (GRCm38)	missense	probably benign	0.06
R6788:Otog	UTSW	7	46,298,317 (GRCm38)	missense	probably damaging	1.00
R6859:Otog	UTSW	7	46,273,781 (GRCm38)	missense	probably damaging	0.96
R7033:Otog	UTSW	7	46,267,398 (GRCm38)	critical splice donor site	probably null
R7071:Otog	UTSW	7	46,267,323 (GRCm38)	missense	probably damaging	1.00
R7084:Otog	UTSW	7	46,298,566 (GRCm38)	nonsense	probably null
R7116:Otog	UTSW	7	46,298,265 (GRCm38)	missense	probably damaging	0.99
R7202:Otog	UTSW	7	46,288,050 (GRCm38)	missense	probably damaging	0.97
R7365:Otog	UTSW	7	46,298,308 (GRCm38)	missense	probably damaging	1.00
R7468:Otog	UTSW	7	46,264,119 (GRCm38)	missense	probably benign
R7475:Otog	UTSW	7	46,267,276 (GRCm38)	missense	probably damaging	0.99
R7502:Otog	UTSW	7	46,298,615 (GRCm38)	missense	probably damaging	1.00
R7558:Otog	UTSW	7	46,303,160 (GRCm38)	missense	probably damaging	0.99
R7577:Otog	UTSW	7	46,287,855 (GRCm38)	missense	possibly damaging	0.62
R7651:Otog	UTSW	7	46,241,761 (GRCm38)	missense	probably benign	0.00
R7689:Otog	UTSW	7	46,252,056 (GRCm38)	missense	probably damaging	1.00
R7806:Otog	UTSW	7	46,285,776 (GRCm38)	missense	probably benign
R7933:Otog	UTSW	7	46,310,147 (GRCm38)	missense	probably damaging	1.00
R8021:Otog	UTSW	7	46,267,342 (GRCm38)	missense	probably damaging	0.98
R8082:Otog	UTSW	7	46,289,719 (GRCm38)	missense	probably damaging	1.00
R8531:Otog	UTSW	7	46,252,049 (GRCm38)	missense	probably damaging	0.99
R8772:Otog	UTSW	7	46,284,928 (GRCm38)	missense	probably damaging	1.00
R8816:Otog	UTSW	7	46,301,481 (GRCm38)	missense	possibly damaging	0.92
R8842:Otog	UTSW	7	46,246,524 (GRCm38)	missense	probably damaging	1.00
R8987:Otog	UTSW	7	46,287,454 (GRCm38)	missense	probably benign	0.43
R8988:Otog	UTSW	7	46,310,147 (GRCm38)	missense	probably damaging	1.00
R9010:Otog	UTSW	7	46,300,470 (GRCm38)	missense	probably benign	0.00
R9025:Otog	UTSW	7	46,288,096 (GRCm38)	missense	probably benign	0.13
R9131:Otog	UTSW	7	46,303,173 (GRCm38)	nonsense	probably null
R9179:Otog	UTSW	7	46,288,461 (GRCm38)	missense	possibly damaging	0.65
R9334:Otog	UTSW	7	46,259,929 (GRCm38)	missense	possibly damaging	0.95
R9365:Otog	UTSW	7	46,271,264 (GRCm38)	missense	probably damaging	1.00
R9408:Otog	UTSW	7	46,267,297 (GRCm38)	missense	possibly damaging	0.79
R9418:Otog	UTSW	7	46,288,600 (GRCm38)	missense	probably benign	0.41
R9465:Otog	UTSW	7	46,305,875 (GRCm38)	missense	possibly damaging	0.80
R9496:Otog	UTSW	7	46,241,081 (GRCm38)	missense	unknown
R9632:Otog	UTSW	7	46,265,719 (GRCm38)	missense	probably benign	0.27
R9656:Otog	UTSW	7	46,310,143 (GRCm38)	missense	probably damaging	1.00
RF024:Otog	UTSW	7	46,287,669 (GRCm38)	missense	probably damaging	1.00
X0062:Otog	UTSW	7	46,259,921 (GRCm38)	missense	probably damaging	1.00
Z1177:Otog	UTSW	7	46,289,740 (GRCm38)	missense	probably damaging	1.00
Z1177:Otog	UTSW	7	46,274,538 (GRCm38)	missense	probably damaging	1.00
Z1177:Otog	UTSW	7	46,262,852 (GRCm38)	missense	possibly damaging	0.80
Z1177:Otog	UTSW	7	46,309,985 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGAAGGACCCTGGGCTATTATAGGGA -3'
(R):5'- GCCCCAGCCTGTCACCATTGAA -3'

Sequencing Primer
(F):5'- caccctgaccaaaagcaac -3'
(R):5'- AATTCAACTCTCTGGCACCTG -3'

Posted On

2013-10-16