Mutagenetix > Incidental Mutations

Incidental Mutation 'R0837:Zfy1'

78018

Institutional Source

Beutler Lab

Gene Symbol

Zfy1

Ensembl Gene

ENSMUSG00000053211

Gene Name

zinc finger protein 1, Y-linked

Synonyms

Zfy-1

MMRRC Submission

039016-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.047) question?

Stock #

R0837 (G1)

Quality Score

125

Status

Not validated

Chromosome

Chromosomal Location

725128-797409 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 725850 bp

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 638 (Y638*)

Ref Sequence

ENSEMBL: ENSMUSP00000140600 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000065545] [ENSMUST00000189888]

Predicted Effect

probably null
Transcript: ENSMUST00000065545
AA Change: Y638*

SMART Domains

Protein: ENSMUSP00000069364
Gene: ENSMUSG00000053211
AA Change: Y638*

Domain	Start	End	E-Value	Type
low complexity region	3	13	N/A	INTRINSIC
Pfam:Zfx_Zfy_act	68	388	1.1e-109	PFAM
ZnF_C2H2	403	425	1.95e-3	SMART
ZnF_C2H2	434	456	1.26e-2	SMART
ZnF_C2H2	466	488	1.05e1	SMART
ZnF_C2H2	497	520	1.41e0	SMART
ZnF_C2H2	526	548	3.69e-4	SMART
ZnF_C2H2	554	577	3.63e-3	SMART
ZnF_C2H2	583	605	8.98e0	SMART
ZnF_C2H2	611	634	3.58e-2	SMART
ZnF_C2H2	640	662	2.95e-3	SMART
ZnF_C2H2	668	691	3.47e0	SMART
ZnF_C2H2	697	719	1.45e-2	SMART
ZnF_C2H2	725	748	1.2e-3	SMART
ZnF_C2H2	754	776	5.81e-2	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000189888
AA Change: Y638*

SMART Domains

Protein: ENSMUSP00000140600
Gene: ENSMUSG00000053211
AA Change: Y638*

Domain	Start	End	E-Value	Type
low complexity region	3	13	N/A	INTRINSIC
Pfam:Zfx_Zfy_act	67	388	1.2e-141	PFAM
ZnF_C2H2	403	425	1.95e-3	SMART
ZnF_C2H2	434	456	1.26e-2	SMART
ZnF_C2H2	466	488	1.05e1	SMART
ZnF_C2H2	497	520	1.41e0	SMART
ZnF_C2H2	526	548	3.69e-4	SMART
ZnF_C2H2	554	577	3.63e-3	SMART
ZnF_C2H2	583	605	8.98e0	SMART
ZnF_C2H2	611	634	3.58e-2	SMART
ZnF_C2H2	640	662	2.95e-3	SMART
ZnF_C2H2	668	691	3.47e0	SMART
ZnF_C2H2	697	719	1.45e-2	SMART
ZnF_C2H2	725	748	1.2e-3	SMART
ZnF_C2H2	754	776	5.81e-2	SMART

Coding Region Coverage

1x: 99.4%
3x: 98.8%
10x: 97.4%
20x: 94.8%

Validation Efficiency

96% (44/46)

Allele List at MGI

Other mutations in this stock

Total: 44 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930563I02Rik	T	C	14: 60,095,926		probably benign	Het
4930579C12Rik	A	G	9: 89,168,207		noncoding transcript	Het
Adam34	T	A	8: 43,651,500	K369N	probably benign	Het
Ap1g1	T	C	8: 109,851,065	W481R	probably damaging	Het
Bicdl1	G	A	5: 115,731,292	P26S	probably benign	Het
Cacna1c	A	T	6: 118,630,270	C1224*	probably null	Het
Cpsf2	C	T	12: 101,997,242		probably benign	Het
Cyp11b2	G	A	15: 74,853,641	R210W	probably damaging	Het
Dock7	C	T	4: 98,989,258	V1048I	probably benign	Het
Dync2h1	C	A	9: 7,077,979	A2908S	probably benign	Het
Elane	A	G	10: 79,887,108	D116G	probably damaging	Het
Epb41l2	C	T	10: 25,507,816	R153C	probably damaging	Het
Gucy2c	G	A	6: 136,722,420	P617L	probably damaging	Het
Hist2h2ab	G	A	3: 96,220,123	A70T	probably damaging	Het
Kcnq4	T	A	4: 120,746,861	I106L	probably benign	Het
Man2b1	A	G	8: 85,096,829	N931D	possibly damaging	Het
Mthfs	A	G	9: 89,215,390	E100G	probably damaging	Het
Mto1	A	T	9: 78,473,790	I639F	probably damaging	Het
Myo15	A	G	11: 60,487,251	E177G	probably damaging	Het
Naip5	T	A	13: 100,230,743	M282L	probably benign	Het
Olfr433	A	G	1: 174,042,487	D179G	probably damaging	Het
Pik3c2g	G	A	6: 139,957,699		probably benign	Het
Prl7d1	T	A	13: 27,714,338	M64L	probably benign	Het
Prnp	A	T	2: 131,936,524	N32I	probably damaging	Het
Ptpn11	C	T	5: 121,149,111	V406I	probably benign	Het
Rab40c	G	A	17: 25,884,693	T151I	probably damaging	Het
Rb1cc1	T	A	1: 6,234,271		probably null	Het
Rnf145	T	C	11: 44,524,988	V10A	probably benign	Het
Rtn4rl2	T	C	2: 84,880,692	N70S	probably damaging	Het
Scaper	A	T	9: 55,859,042	C483*	probably null	Het
Sema4a	T	A	3: 88,453,098	Q58L	possibly damaging	Het
Slf1	T	C	13: 77,100,948		probably null	Het
Sycp1	G	T	3: 102,915,245	N364K	probably benign	Het
Tenm4	T	C	7: 96,896,275		probably benign	Het
Tnfaip2	A	G	12: 111,450,707	T537A	probably damaging	Het
Trappc12	A	G	12: 28,703,597	I573T	possibly damaging	Het
Ugt2b38	G	A	5: 87,411,773	T420I	probably damaging	Het
Ulk1	A	T	5: 110,789,545		probably benign	Het
Unc80	G	A	1: 66,648,944	C2367Y	possibly damaging	Het
Usp7	C	A	16: 8,703,502	G135C	probably damaging	Het
Vmn2r103	A	G	17: 19,793,927	Y327C	probably damaging	Het
Vmn2r28	T	A	7: 5,488,027	H407L	probably damaging	Het
Zfp804a	A	C	2: 82,259,162	T1112P	probably damaging	Het
Zfr2	A	G	10: 81,245,408	K431E	probably damaging	Het

Other mutations in Zfy1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0149:Zfy1	UTSW	Y	726121	missense	possibly damaging	0.95
R0361:Zfy1	UTSW	Y	726121	missense	possibly damaging	0.95
R0529:Zfy1	UTSW	Y	726040	missense	probably damaging	1.00
R0945:Zfy1	UTSW	Y	725983	missense	probably damaging	0.98
R1163:Zfy1	UTSW	Y	725611	missense	probably damaging	0.98
R1394:Zfy1	UTSW	Y	725957	missense	possibly damaging	0.79
R1806:Zfy1	UTSW	Y	725620	missense	possibly damaging	0.88
R1928:Zfy1	UTSW	Y	729733	missense	unknown
R2374:Zfy1	UTSW	Y	726391	missense	probably damaging	0.99
R2374:Zfy1	UTSW	Y	726392	missense	possibly damaging	0.77
R2889:Zfy1	UTSW	Y	726307	missense	possibly damaging	0.95
R2980:Zfy1	UTSW	Y	739054	missense	unknown
R3437:Zfy1	UTSW	Y	726357	missense	possibly damaging	0.82
R4454:Zfy1	UTSW	Y	725518	missense	possibly damaging	0.86
R4529:Zfy1	UTSW	Y	726511	missense	possibly damaging	0.93
R4656:Zfy1	UTSW	Y	729626	missense	unknown
R5049:Zfy1	UTSW	Y	726004	missense	possibly damaging	0.93
R5087:Zfy1	UTSW	Y	732964	missense	unknown
R5347:Zfy1	UTSW	Y	725950	missense	possibly damaging	0.90
R5428:Zfy1	UTSW	Y	726205	missense	possibly damaging	0.95
R5825:Zfy1	UTSW	Y	726531	missense	possibly damaging	0.85
R6256:Zfy1	UTSW	Y	738765	missense	unknown
R7065:Zfy1	UTSW	Y	725428	missense	probably benign	0.33
R7134:Zfy1	UTSW	Y	725788	missense	probably damaging	0.99
R7185:Zfy1	UTSW	Y	725464	missense	possibly damaging	0.53
R7358:Zfy1	UTSW	Y	735141	missense	unknown
R7513:Zfy1	UTSW	Y	759852	missense	unknown
R7747:Zfy1	UTSW	Y	725496	nonsense	probably null
R7900:Zfy1	UTSW	Y	725519	missense	possibly damaging	0.53
R8052:Zfy1	UTSW	Y	726004	missense	possibly damaging	0.93
R8377:Zfy1	UTSW	Y	725723	missense	possibly damaging	0.90
R8795:Zfy1	UTSW	Y	738945	missense	unknown
R8854:Zfy1	UTSW	Y	726501	missense	possibly damaging	0.71

Predicted Primers

PCR Primer
(F):5'- TGGCGCTTAAAACCTGAGGCATC -3'
(R):5'- CTGAAGTGTGGCATCTGTCTCCTG -3'

Sequencing Primer
(F):5'- ATGCGTTTGAAGCTCACACTG -3'
(R):5'- CTGACTTTCTCAGATAACAAAGAGGC -3'

Posted On

2013-10-16