Incidental Mutation 'I0000:Klf5'
ID 7841
Institutional Source Beutler Lab
Gene Symbol Klf5
Ensembl Gene ENSMUSG00000005148
Gene Name Kruppel-like factor 5
Synonyms IKLF, Bteb2, 4930520J07Rik, CKLF
Accession Numbers
Is this an essential gene? Essential (E-score: 1.000) question?
Stock # I0000 (G3) of strain 635
Quality Score
Status Validated
Chromosome 14
Chromosomal Location 99298691-99315036 bp(+) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) C to T at 99303475 bp (GRCm38)
Zygosity Homozygous
Amino Acid Change Threonine to Methionine at position 307 (T307M)
Ref Sequence ENSEMBL: ENSMUSP00000154786 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000005279] [ENSMUST00000226784]
AlphaFold Q9Z0Z7
Predicted Effect probably damaging
Transcript: ENSMUST00000005279
AA Change: T387M

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000005279
Gene: ENSMUSG00000005148
AA Change: T387M

DomainStartEndE-ValueType
low complexity region 53 65 N/A INTRINSIC
low complexity region 166 173 N/A INTRINSIC
low complexity region 290 301 N/A INTRINSIC
ZnF_C2H2 362 386 3.83e-2 SMART
ZnF_C2H2 392 416 2.47e-5 SMART
ZnF_C2H2 422 444 1.2e-3 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000226784
AA Change: T307M

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
Meta Mutation Damage Score 0.6467 question?
Coding Region Coverage
  • 1x: 90.9%
  • 3x: 86.6%
Validation Efficiency 67% (62/92)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. The encoded protein is a transcriptional activator that binds directly to a specific recognition motif in the promoters of target genes. This protein acts downstream of multiple different signaling pathways and is regulated by post-translational modification. It may participate in both promoting and suppressing cell proliferation. Expression of this gene may be changed in a variety of different cancers and in cardiovascular disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]
PHENOTYPE: Homozygous null mice die during gestation, while heterozygotes exhibit abnormal cardiovascular remodeling after external stress. Mice homozygous for a floxed allele activated in the prostate exhibit increased cell proliferation and hyperplasia in the prostate without neoplasia. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 27 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Acacb T C 5: 114,238,655 V1933A probably damaging Het
Acsm4 T A 7: 119,711,192 F467I probably damaging Het
Ankrd55 A T 13: 112,348,725 probably benign Het
Bfsp1 T C 2: 143,845,968 Y179C probably damaging Het
Ccdc61 A G 7: 18,903,549 I51T probably damaging Het
Ccdc81 A G 7: 89,898,051 L43P probably damaging Het
Ddias A G 7: 92,866,640 V15A possibly damaging Het
Dpp6 A T 5: 27,398,922 T62S probably benign Het
Ereg G A 5: 91,089,209 C129Y probably benign Het
Fras1 G A 5: 96,740,829 G2745S probably damaging Het
Gzf1 C T 2: 148,686,620 probably benign Het
Herc2 T A 7: 56,136,729 probably benign Het
Hsd17b4 A G 18: 50,160,228 D278G probably benign Homo
Ifitm3 A G 7: 141,010,528 S40P possibly damaging Het
Lnpep A T 17: 17,578,971 C141S probably damaging Homo
Mmp19 A T 10: 128,798,460 D362V probably benign Het
Olfr1245 T C 2: 89,575,153 Y191C probably damaging Het
Pnpla6 G A 8: 3,542,322 A1222T probably benign Het
Rbm26 C A 14: 105,153,567 R161L unknown Homo
Selenon G A 4: 134,542,701 probably benign Het
Sept11 A G 5: 93,165,259 T322A probably benign Het
Sh3bp4 T A 1: 89,137,796 D37E probably benign Het
Tango2 G A 16: 18,312,666 R80W possibly damaging Homo
Tjap1 C T 17: 46,259,029 C345Y probably damaging Homo
Wdr62 T C 7: 30,245,327 D455G probably benign Het
Zbtb48 A G 4: 152,019,858 I671T probably benign Het
Zfp318 T C 17: 46,399,559 L736P probably damaging Homo
Other mutations in Klf5
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01295:Klf5 APN 14 99301721 missense probably benign 0.01
IGL02380:Klf5 APN 14 99301458 missense possibly damaging 0.67
Bernie UTSW 14 99302230 missense probably damaging 1.00
R0133:Klf5 UTSW 14 99301882 missense probably benign
R1672:Klf5 UTSW 14 99301550 missense probably damaging 0.98
R1914:Klf5 UTSW 14 99301921 missense probably benign 0.01
R2193:Klf5 UTSW 14 99298970 unclassified probably benign
R3892:Klf5 UTSW 14 99299073 missense probably benign 0.00
R4446:Klf5 UTSW 14 99302230 missense probably damaging 1.00
R5437:Klf5 UTSW 14 99301459 nonsense probably null
R5707:Klf5 UTSW 14 99301508 missense probably benign
R6475:Klf5 UTSW 14 99301381 missense probably benign 0.00
R6552:Klf5 UTSW 14 99301642 missense probably benign
R6982:Klf5 UTSW 14 99313235 missense probably damaging 1.00
R7250:Klf5 UTSW 14 99299019 missense probably benign 0.00
R7643:Klf5 UTSW 14 99313178 missense possibly damaging 0.88
R7938:Klf5 UTSW 14 99299008 missense probably damaging 0.98
R8272:Klf5 UTSW 14 99302104 missense possibly damaging 0.67
R8396:Klf5 UTSW 14 99302234 missense possibly damaging 0.95
R8898:Klf5 UTSW 14 99301486 missense probably damaging 0.99
R9015:Klf5 UTSW 14 99303483 makesense probably null
R9251:Klf5 UTSW 14 99301388 missense possibly damaging 0.95
R9560:Klf5 UTSW 14 99301598 missense probably benign 0.06
R9717:Klf5 UTSW 14 99301753 missense probably damaging 1.00
Posted On 2012-11-05