Mutagenetix > Incidental Mutations

Incidental Mutation 'R0927:Ifit2'

80575

Institutional Source

Beutler Lab

Gene Symbol

Ifit2

Ensembl Gene

ENSMUSG00000045932

Gene Name

interferon-induced protein with tetratricopeptide repeats 2

Synonyms

Ifi54

MMRRC Submission

039074-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R0927 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

34550694-34576419 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 34573584 bp

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 175 (T175S)

Ref Sequence

ENSEMBL: ENSMUSP00000099890 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000102826] [ENSMUST00000149829]

Predicted Effect

probably benign
Transcript: ENSMUST00000102826
AA Change: T175S

PolyPhen 2 Score 0.027 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000099890
Gene: ENSMUSG00000045932
AA Change: T175S

Domain	Start	End	E-Value	Type
Pfam:TPR_2	95	127	4e-4	PFAM
Pfam:TPR_8	95	127	3.8e-4	PFAM
Blast:TPR	138	171	7e-11	BLAST
Blast:TPR	172	208	2e-9	BLAST
low complexity region	211	222	N/A	INTRINSIC
Pfam:TPR_19	225	286	4e-8	PFAM
low complexity region	396	406	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000149829

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.0%
20x: 93.7%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit increased susuceptibility to VSV infection with increased lethality and brain viral titer. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb8	T	C	5: 24,402,319	L363P	probably damaging	Het
Adam12	T	A	7: 133,998,230	H85L	probably damaging	Het
Adam34	A	T	8: 43,651,584	H341Q	probably damaging	Het
Adam7	A	G	14: 68,516,684	L322P	probably damaging	Het
Adcy5	T	A	16: 35,156,243	S49T	probably benign	Het
Arfgap2	T	C	2: 91,273,805	S374P	probably benign	Het
Arpp19	G	A	9: 75,037,685		probably benign	Het
Baz1a	T	C	12: 54,894,988	K1478E	probably damaging	Het
Cdc27	G	T	11: 104,505,641	A812E	possibly damaging	Het
Chtf8	G	A	8: 106,885,518	T263I	probably damaging	Het
Clcn6	T	C	4: 148,029,392	N70D	probably benign	Het
Cntnap5c	A	T	17: 58,042,558	T289S	possibly damaging	Het
Dzip3	T	C	16: 48,975,477	N177S	probably damaging	Het
Edar	G	T	10: 58,629,491		probably null	Het
Enam	T	A	5: 88,494,060	N244K	possibly damaging	Het
Fbxw10	A	G	11: 62,876,944	K874E	probably damaging	Het
Glra3	A	G	8: 56,125,204	E432G	possibly damaging	Het
Gm13084	A	T	4: 143,812,808	D38E	probably benign	Het
Gm13088	T	A	4: 143,654,220	H411L	possibly damaging	Het
Gm5346	A	T	8: 43,625,123	M688K	probably benign	Het
Grin3b	G	A	10: 79,971,228	R110Q	probably benign	Het
Herc3	T	A	6: 58,868,763	V423D	possibly damaging	Het
Kcnj8	T	G	6: 142,565,901	I327L	possibly damaging	Het
Kcns2	A	T	15: 34,839,096	I202F	probably benign	Het
Kif12	T	C	4: 63,168,773	R305G	possibly damaging	Het
Limch1	A	G	5: 66,997,233	D362G	probably damaging	Het
Lrba	T	C	3: 86,780,233	I2815T	probably damaging	Het
Lrrtm1	G	T	6: 77,244,860	M433I	probably damaging	Het
Myh7b	A	G	2: 155,620,120	D312G	probably damaging	Het
Nrxn1	A	T	17: 90,037,330	I382N	probably damaging	Het
Nudt6	C	T	3: 37,405,353	R161H	probably benign	Het
Olfr1490	T	A	19: 13,654,452	W8R	probably damaging	Het
Olfr734	A	T	14: 50,320,729	Y35*	probably null	Het
Olfr744	A	C	14: 50,618,587	M122L	possibly damaging	Het
Olfr857	C	T	9: 19,713,649	A274V	probably benign	Het
Pmf1	A	T	3: 88,396,062	V64D	probably damaging	Het
Pomgnt1	T	A	4: 116,151,851	V29D	probably damaging	Het
Prex1	C	T	2: 166,586,537	A925T	probably benign	Het
Pus3	A	G	9: 35,565,031	Y72C	probably damaging	Het
Rnf20	T	C	4: 49,642,176	S247P	probably damaging	Het
Rnf213	T	A	11: 119,414,570	D542E	probably benign	Het
Sidt1	T	C	16: 44,243,532	D786G	probably benign	Het
Sirt6	A	T	10: 81,622,641	D219E	probably damaging	Het
Slc36a2	A	T	11: 55,181,585	I67N	probably damaging	Het
Slc47a1	A	G	11: 61,373,422	F57S	probably damaging	Het
Spg11	T	A	2: 122,094,487	T756S	probably damaging	Het
Sptbn1	C	A	11: 30,121,591	R1447L	probably damaging	Het
Tcf7l2	A	G	19: 55,918,955	M340V	probably damaging	Het
Thap1	T	C	8: 26,162,705	V157A	probably benign	Het
Ubash3b	G	A	9: 41,023,557	Q354*	probably null	Het
Ubtd1	G	A	19: 42,032,021	W68*	probably null	Het
Wdr64	G	T	1: 175,793,081	R793L	probably damaging	Het
Zbtb24	C	T	10: 41,451,436	T106I	probably benign	Het
Zbtb26	T	C	2: 37,436,325	N233S	possibly damaging	Het
Zcchc24	A	T	14: 25,757,161	N99K	possibly damaging	Het

Other mutations in Ifit2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01099:Ifit2	APN	19	34573302	missense	probably damaging	1.00
IGL02261:Ifit2	APN	19	34574224	missense	probably damaging	1.00
IGL02375:Ifit2	APN	19	34574337	missense	probably benign	0.01
Pushup	UTSW	19	34574045	missense	probably benign	0.38
R0017:Ifit2	UTSW	19	34573573	missense	probably damaging	1.00
R0017:Ifit2	UTSW	19	34573573	missense	probably damaging	1.00
R0682:Ifit2	UTSW	19	34573612	missense	probably benign	0.13
R1462:Ifit2	UTSW	19	34573186	missense	probably null	0.12
R1462:Ifit2	UTSW	19	34573186	missense	probably null	0.12
R1526:Ifit2	UTSW	19	34573202	missense	probably benign	0.00
R2084:Ifit2	UTSW	19	34573350	missense	probably damaging	1.00
R3971:Ifit2	UTSW	19	34574041	missense	probably benign	0.00
R4008:Ifit2	UTSW	19	34574045	missense	probably benign	0.38
R4010:Ifit2	UTSW	19	34574045	missense	probably benign	0.38
R4011:Ifit2	UTSW	19	34574045	missense	probably benign	0.38
R4359:Ifit2	UTSW	19	34573144	missense	possibly damaging	0.85
R5179:Ifit2	UTSW	19	34573576	missense	probably damaging	1.00
R5240:Ifit2	UTSW	19	34574396	missense	probably benign	0.02
R5424:Ifit2	UTSW	19	34574058	missense	probably benign	0.19
R5528:Ifit2	UTSW	19	34573537	missense	possibly damaging	0.63
R6605:Ifit2	UTSW	19	34573497	nonsense	probably null
R7172:Ifit2	UTSW	19	34573494	missense	probably benign	0.24
R7424:Ifit2	UTSW	19	34573198	missense	probably benign	0.37
X0023:Ifit2	UTSW	19	34574250	missense	possibly damaging	0.59
X0064:Ifit2	UTSW	19	34573923	missense	probably benign	0.06

Predicted Primers

PCR Primer
(F):5'- AGAAGTCTGGTCACCTGGGGAAAC -3'
(R):5'- GCAATAAAACCTGGCTGCCCTGAG -3'

Sequencing Primer
(F):5'- GTCTACTATCACATGGGCCAG -3'
(R):5'- TAAGGCTTCTTCAACCAGCG -3'

Posted On

2013-11-07