Mutagenetix > Incidental Mutation

Incidental Mutation 'R0927:Ifit2'

80575

Institutional Source

Beutler Lab

Gene Symbol

Ifit2

Ensembl Gene

ENSMUSG00000045932

Gene Name

interferon-induced protein with tetratricopeptide repeats 2

Synonyms

Ifi54

MMRRC Submission

039074-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.062) question?

Stock #

R0927 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

34528094-34553819 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 34550984 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 175 (T175S)

Ref Sequence

ENSEMBL: ENSMUSP00000099890 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000102826] [ENSMUST00000149829]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000102826
AA Change: T175S

PolyPhen 2 Score 0.027 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000099890
Gene: ENSMUSG00000045932
AA Change: T175S

Domain	Start	End	E-Value	Type
Pfam:TPR_2	95	127	4e-4	PFAM
Pfam:TPR_8	95	127	3.8e-4	PFAM
Blast:TPR	138	171	7e-11	BLAST
Blast:TPR	172	208	2e-9	BLAST
low complexity region	211	222	N/A	INTRINSIC
Pfam:TPR_19	225	286	4e-8	PFAM
low complexity region	396	406	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000149829

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.0%
20x: 93.7%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit increased susuceptibility to VSV infection with increased lethality and brain viral titer. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb8	T	C	5: 24,607,317 (GRCm39)	L363P	probably damaging	Het
Adam12	T	A	7: 133,599,959 (GRCm39)	H85L	probably damaging	Het
Adam34	A	T	8: 44,104,621 (GRCm39)	H341Q	probably damaging	Het
Adam34l	A	T	8: 44,078,160 (GRCm39)	M688K	probably benign	Het
Adam7	A	G	14: 68,754,133 (GRCm39)	L322P	probably damaging	Het
Adcy5	T	A	16: 34,976,613 (GRCm39)	S49T	probably benign	Het
Arfgap2	T	C	2: 91,104,150 (GRCm39)	S374P	probably benign	Het
Arpp19	G	A	9: 74,944,967 (GRCm39)		probably benign	Het
Baz1a	T	C	12: 54,941,773 (GRCm39)	K1478E	probably damaging	Het
Cdc27	G	T	11: 104,396,467 (GRCm39)	A812E	possibly damaging	Het
Chtf8	G	A	8: 107,612,150 (GRCm39)	T263I	probably damaging	Het
Clcn6	T	C	4: 148,113,849 (GRCm39)	N70D	probably benign	Het
Cntnap5c	A	T	17: 58,349,553 (GRCm39)	T289S	possibly damaging	Het
Dzip3	T	C	16: 48,795,840 (GRCm39)	N177S	probably damaging	Het
Edar	G	T	10: 58,465,313 (GRCm39)		probably null	Het
Enam	T	A	5: 88,641,919 (GRCm39)	N244K	possibly damaging	Het
Fbxw10	A	G	11: 62,767,770 (GRCm39)	K874E	probably damaging	Het
Glra3	A	G	8: 56,578,239 (GRCm39)	E432G	possibly damaging	Het
Grin3b	G	A	10: 79,807,062 (GRCm39)	R110Q	probably benign	Het
Herc3	T	A	6: 58,845,748 (GRCm39)	V423D	possibly damaging	Het
Kcnj8	T	G	6: 142,511,627 (GRCm39)	I327L	possibly damaging	Het
Kcns2	A	T	15: 34,839,242 (GRCm39)	I202F	probably benign	Het
Kif12	T	C	4: 63,087,010 (GRCm39)	R305G	possibly damaging	Het
Limch1	A	G	5: 67,154,576 (GRCm39)	D362G	probably damaging	Het
Lrba	T	C	3: 86,687,540 (GRCm39)	I2815T	probably damaging	Het
Lrrtm1	G	T	6: 77,221,843 (GRCm39)	M433I	probably damaging	Het
Myh7b	A	G	2: 155,462,040 (GRCm39)	D312G	probably damaging	Het
Nrxn1	A	T	17: 90,344,758 (GRCm39)	I382N	probably damaging	Het
Nudt6	C	T	3: 37,459,502 (GRCm39)	R161H	probably benign	Het
Or10w1	T	A	19: 13,631,816 (GRCm39)	W8R	probably damaging	Het
Or11g2	A	C	14: 50,856,044 (GRCm39)	M122L	possibly damaging	Het
Or4m1	A	T	14: 50,558,186 (GRCm39)	Y35*	probably null	Het
Or7e166	C	T	9: 19,624,945 (GRCm39)	A274V	probably benign	Het
Pmf1	A	T	3: 88,303,369 (GRCm39)	V64D	probably damaging	Het
Pomgnt1	T	A	4: 116,009,048 (GRCm39)	V29D	probably damaging	Het
Pramel22	T	A	4: 143,380,790 (GRCm39)	H411L	possibly damaging	Het
Pramel26	A	T	4: 143,539,378 (GRCm39)	D38E	probably benign	Het
Prex1	C	T	2: 166,428,457 (GRCm39)	A925T	probably benign	Het
Pus3	A	G	9: 35,476,327 (GRCm39)	Y72C	probably damaging	Het
Rnf20	T	C	4: 49,642,176 (GRCm39)	S247P	probably damaging	Het
Rnf213	T	A	11: 119,305,396 (GRCm39)	D542E	probably benign	Het
Sidt1	T	C	16: 44,063,895 (GRCm39)	D786G	probably benign	Het
Sirt6	A	T	10: 81,458,475 (GRCm39)	D219E	probably damaging	Het
Slc36a2	A	T	11: 55,072,411 (GRCm39)	I67N	probably damaging	Het
Slc47a1	A	G	11: 61,264,248 (GRCm39)	F57S	probably damaging	Het
Spg11	T	A	2: 121,924,968 (GRCm39)	T756S	probably damaging	Het
Sptbn1	C	A	11: 30,071,591 (GRCm39)	R1447L	probably damaging	Het
Tcf7l2	A	G	19: 55,907,387 (GRCm39)	M340V	probably damaging	Het
Thap1	T	C	8: 26,652,733 (GRCm39)	V157A	probably benign	Het
Ubash3b	G	A	9: 40,934,853 (GRCm39)	Q354*	probably null	Het
Ubtd1	G	A	19: 42,020,460 (GRCm39)	W68*	probably null	Het
Wdr64	G	T	1: 175,620,647 (GRCm39)	R793L	probably damaging	Het
Zbtb24	C	T	10: 41,327,432 (GRCm39)	T106I	probably benign	Het
Zbtb26	T	C	2: 37,326,337 (GRCm39)	N233S	possibly damaging	Het
Zcchc24	A	T	14: 25,757,585 (GRCm39)	N99K	possibly damaging	Het

Other mutations in Ifit2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01099:Ifit2	APN	19	34,550,702 (GRCm39)	missense	probably damaging	1.00
IGL02261:Ifit2	APN	19	34,551,624 (GRCm39)	missense	probably damaging	1.00
IGL02375:Ifit2	APN	19	34,551,737 (GRCm39)	missense	probably benign	0.01
Pushup	UTSW	19	34,551,445 (GRCm39)	missense	probably benign	0.38
R0017:Ifit2	UTSW	19	34,550,973 (GRCm39)	missense	probably damaging	1.00
R0017:Ifit2	UTSW	19	34,550,973 (GRCm39)	missense	probably damaging	1.00
R0682:Ifit2	UTSW	19	34,551,012 (GRCm39)	missense	probably benign	0.13
R1462:Ifit2	UTSW	19	34,550,586 (GRCm39)	missense	probably null	0.12
R1462:Ifit2	UTSW	19	34,550,586 (GRCm39)	missense	probably null	0.12
R1526:Ifit2	UTSW	19	34,550,602 (GRCm39)	missense	probably benign	0.00
R2084:Ifit2	UTSW	19	34,550,750 (GRCm39)	missense	probably damaging	1.00
R3971:Ifit2	UTSW	19	34,551,441 (GRCm39)	missense	probably benign	0.00
R4008:Ifit2	UTSW	19	34,551,445 (GRCm39)	missense	probably benign	0.38
R4010:Ifit2	UTSW	19	34,551,445 (GRCm39)	missense	probably benign	0.38
R4011:Ifit2	UTSW	19	34,551,445 (GRCm39)	missense	probably benign	0.38
R4359:Ifit2	UTSW	19	34,550,544 (GRCm39)	missense	possibly damaging	0.85
R5179:Ifit2	UTSW	19	34,550,976 (GRCm39)	missense	probably damaging	1.00
R5240:Ifit2	UTSW	19	34,551,796 (GRCm39)	missense	probably benign	0.02
R5424:Ifit2	UTSW	19	34,551,458 (GRCm39)	missense	probably benign	0.19
R5528:Ifit2	UTSW	19	34,550,937 (GRCm39)	missense	possibly damaging	0.63
R6605:Ifit2	UTSW	19	34,550,897 (GRCm39)	nonsense	probably null
R7172:Ifit2	UTSW	19	34,550,894 (GRCm39)	missense	probably benign	0.24
R7424:Ifit2	UTSW	19	34,550,598 (GRCm39)	missense	probably benign	0.37
R8090:Ifit2	UTSW	19	34,550,662 (GRCm39)	missense	possibly damaging	0.70
R8356:Ifit2	UTSW	19	34,550,908 (GRCm39)	nonsense	probably null
R8553:Ifit2	UTSW	19	34,550,538 (GRCm39)	missense	probably benign	0.00
X0023:Ifit2	UTSW	19	34,551,650 (GRCm39)	missense	possibly damaging	0.59
X0064:Ifit2	UTSW	19	34,551,323 (GRCm39)	missense	probably benign	0.06

Predicted Primers

PCR Primer
(F):5'- AGAAGTCTGGTCACCTGGGGAAAC -3'
(R):5'- GCAATAAAACCTGGCTGCCCTGAG -3'

Sequencing Primer
(F):5'- GTCTACTATCACATGGGCCAG -3'
(R):5'- TAAGGCTTCTTCAACCAGCG -3'

Posted On

2013-11-07