Mutagenetix > Incidental Mutation

Incidental Mutation 'R0881:Mslnl'

80580

Institutional Source

Beutler Lab

Gene Symbol

Mslnl

Ensembl Gene

ENSMUSG00000041062

Gene Name

mesothelin-like

Synonyms

MMRRC Submission

039048-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R0881 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

25955016-25967304 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 25961939 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Leucine at position 138 (H138L)

Ref Sequence

ENSEMBL: ENSMUSP00000049020 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000047098]

AlphaFold

Q8C160

Predicted Effect

possibly damaging
Transcript: ENSMUST00000047098
AA Change: H138L

PolyPhen 2 Score 0.820 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000049020
Gene: ENSMUSG00000041062
AA Change: H138L

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
Pfam:Mesothelin	29	589	2.8e-70	PFAM
low complexity region	633	653	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000102319

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 99.6%
3x: 98.9%
10x: 97.0%
20x: 93.0%

Validation Efficiency

96% (51/53)

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aasdh	G	A	5: 77,024,130 (GRCm39)	T174M	probably damaging	Het
Abcc9	T	A	6: 142,592,029 (GRCm39)	I732F	probably damaging	Het
Adam10	T	C	9: 70,653,519 (GRCm39)	S248P	probably damaging	Het
Adam18	A	T	8: 25,162,159 (GRCm39)		probably benign	Het
Angel2	G	A	1: 190,669,661 (GRCm39)	E114K	probably damaging	Het
Arhgap29	A	G	3: 121,808,328 (GRCm39)	T1169A	probably damaging	Het
Atp13a2	T	A	4: 140,731,242 (GRCm39)	M759K	probably damaging	Het
Atxn2l	A	G	7: 126,095,768 (GRCm39)	S450P	probably damaging	Het
B3glct	T	A	5: 149,663,034 (GRCm39)	V264E	probably damaging	Het
Bbx	A	G	16: 50,040,963 (GRCm39)		probably benign	Het
Bmp3	A	G	5: 99,020,461 (GRCm39)	N295D	possibly damaging	Het
C9	G	A	15: 6,488,349 (GRCm39)		probably benign	Het
Cacna1c	C	T	6: 118,589,586 (GRCm39)	R1446H	probably damaging	Het
Cdan1	A	T	2: 120,551,466 (GRCm39)	V1039E	probably damaging	Het
Dennd4a	T	C	9: 64,758,665 (GRCm39)		probably null	Het
Ext1	A	G	15: 53,207,879 (GRCm39)	L294P	probably benign	Het
Fsip2	C	A	2: 82,816,617 (GRCm39)	H4117N	possibly damaging	Het
Itga8	C	T	2: 12,267,003 (GRCm39)		probably null	Het
Itln1	G	T	1: 171,360,949 (GRCm39)	H48N	probably benign	Het
Kcna5	T	A	6: 126,511,957 (GRCm39)	H57L	probably benign	Het
Klhdc4	A	T	8: 122,526,226 (GRCm39)	Y304*	probably null	Het
Klhl25	A	G	7: 75,516,027 (GRCm39)	Y6C	probably damaging	Het
Lars1	C	T	18: 42,347,851 (GRCm39)	V991M	probably benign	Het
Med20	T	C	17: 47,922,605 (GRCm39)	M1T	probably null	Het
Mycbp2	T	C	14: 103,457,449 (GRCm39)	I1583V	probably benign	Het
Nipbl	A	C	15: 8,337,096 (GRCm39)	V2093G	probably damaging	Het
Nup98	T	A	7: 101,809,923 (GRCm39)	T536S	probably damaging	Het
Opalin	T	C	19: 41,052,420 (GRCm39)		probably null	Het
Or1j12	T	G	2: 36,343,452 (GRCm39)	L285R	probably damaging	Het
Or51d1	T	A	7: 102,348,291 (GRCm39)	V282D	possibly damaging	Het
Or8g26	A	G	9: 39,095,984 (GRCm39)	K170R	probably benign	Het
Pgm2	A	T	5: 64,250,351 (GRCm39)	T9S	unknown	Het
Piwil2	A	C	14: 70,646,376 (GRCm39)	S387A	probably benign	Het
Polr1c	T	C	17: 46,555,539 (GRCm39)	T240A	possibly damaging	Het
Polr3c	A	G	3: 96,631,163 (GRCm39)	M118T	probably damaging	Het
Pth1r	C	T	9: 110,560,641 (GRCm39)	C42Y	probably damaging	Het
Ptpro	T	A	6: 137,420,592 (GRCm39)	V1007D	probably damaging	Het
Rictor	A	T	15: 6,821,151 (GRCm39)	M1492L	probably benign	Het
Rrbp1	T	A	2: 143,795,173 (GRCm39)	Y1277F	probably benign	Het
Scgb3a2	T	G	18: 43,897,549 (GRCm39)		probably benign	Het
Skint1	G	A	4: 111,886,054 (GRCm39)	S327N	probably benign	Het
Steap4	A	T	5: 8,030,388 (GRCm39)	S415C	probably benign	Het
Tex48	A	G	4: 63,530,228 (GRCm39)		probably benign	Het
Tox2	T	A	2: 163,163,365 (GRCm39)	S502T	probably benign	Het
Usp47	T	A	7: 111,690,643 (GRCm39)	I762K	possibly damaging	Het
Vmn2r53	T	C	7: 12,334,859 (GRCm39)	H267R	probably benign	Het
Wnt2b	A	G	3: 104,860,513 (GRCm39)		probably benign	Het
Xirp1	T	C	9: 119,847,483 (GRCm39)	N28D	possibly damaging	Het
Zeb1	T	C	18: 5,767,138 (GRCm39)	S550P	probably benign	Het

Other mutations in Mslnl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01621:Mslnl	APN	17	25,962,641 (GRCm39)	unclassified	probably benign
IGL01629:Mslnl	APN	17	25,963,749 (GRCm39)	missense	possibly damaging	0.95
IGL02084:Mslnl	APN	17	25,965,125 (GRCm39)	missense	probably benign	0.07
IGL02408:Mslnl	APN	17	25,966,972 (GRCm39)	missense	possibly damaging	0.80
IGL02726:Mslnl	APN	17	25,963,077 (GRCm39)	critical splice donor site	probably null
IGL03387:Mslnl	APN	17	25,963,051 (GRCm39)	missense	probably benign	0.06
R0561:Mslnl	UTSW	17	25,962,177 (GRCm39)	nonsense	probably null
R1295:Mslnl	UTSW	17	25,962,214 (GRCm39)	missense	probably damaging	1.00
R1296:Mslnl	UTSW	17	25,962,214 (GRCm39)	missense	probably damaging	1.00
R1582:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1629:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1630:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1631:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1632:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1794:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1850:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1866:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1876:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1914:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2166:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2241:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2243:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2247:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2282:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2284:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2852:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2877:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2878:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2919:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2920:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3026:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3405:Mslnl	UTSW	17	25,965,155 (GRCm39)	missense	probably damaging	1.00
R3406:Mslnl	UTSW	17	25,965,155 (GRCm39)	missense	probably damaging	1.00
R3411:Mslnl	UTSW	17	25,963,491 (GRCm39)	missense	probably benign	0.05
R3434:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3546:Mslnl	UTSW	17	25,963,943 (GRCm39)	missense	probably damaging	0.98
R3612:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3729:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3730:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3802:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3804:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3894:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3895:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4454:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4455:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4456:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4457:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4561:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4562:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4564:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4600:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4601:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4610:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4704:Mslnl	UTSW	17	25,957,952 (GRCm39)	missense	possibly damaging	0.73
R5155:Mslnl	UTSW	17	25,957,942 (GRCm39)	nonsense	probably null
R5257:Mslnl	UTSW	17	25,965,139 (GRCm39)	missense	probably benign	0.00
R5456:Mslnl	UTSW	17	25,962,133 (GRCm39)	missense	probably damaging	0.98
R5645:Mslnl	UTSW	17	25,956,816 (GRCm39)	missense	possibly damaging	0.95
R6007:Mslnl	UTSW	17	25,965,749 (GRCm39)	missense	probably benign	0.00
R6083:Mslnl	UTSW	17	25,956,876 (GRCm39)	missense	possibly damaging	0.83
R6142:Mslnl	UTSW	17	25,963,531 (GRCm39)	missense	probably damaging	1.00
R6761:Mslnl	UTSW	17	25,965,047 (GRCm39)	missense	probably damaging	1.00
R7058:Mslnl	UTSW	17	25,962,186 (GRCm39)	missense	probably benign	0.03
R7156:Mslnl	UTSW	17	25,962,184 (GRCm39)	missense	probably benign	0.20
R7467:Mslnl	UTSW	17	25,955,895 (GRCm39)	start codon destroyed	probably benign	0.33
R7687:Mslnl	UTSW	17	25,962,157 (GRCm39)	missense	probably damaging	0.97
R7807:Mslnl	UTSW	17	25,965,751 (GRCm39)	missense	probably benign	0.03
R8682:Mslnl	UTSW	17	25,965,962 (GRCm39)	missense	probably benign
R8735:Mslnl	UTSW	17	25,964,062 (GRCm39)	missense	probably benign	0.09
R8742:Mslnl	UTSW	17	25,964,047 (GRCm39)	missense	probably damaging	1.00
R9208:Mslnl	UTSW	17	25,961,694 (GRCm39)	missense	possibly damaging	0.94
R9264:Mslnl	UTSW	17	25,961,506 (GRCm39)	intron	probably benign
RF007:Mslnl	UTSW	17	25,962,202 (GRCm39)	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- GTGACAGTCCCCTTATCATGTGCC -3'
(R):5'- GCAGTCACAATTTGTGCTGCCTCC -3'

Sequencing Primer
(F):5'- ATCATGTGCCTGCCAGTG -3'
(R):5'- TCTGAAGCACTGAGCTGTAGG -3'

Posted On

2013-11-07