Mutagenetix > Incidental Mutation

Incidental Mutation 'R0882:Zfp820'

80679

Institutional Source

Beutler Lab

Gene Symbol

Zfp820

Ensembl Gene

ENSMUSG00000069743

Gene Name

zinc finger protein 820

Synonyms

2610036F08Rik

MMRRC Submission

039049-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.105) question?

Stock #

R0882 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

22035857-22064740 bp(-) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

A to G at 22042817 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000081158 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000084141]

AlphaFold

A0A3B2W7H5

Predicted Effect

probably benign
Transcript: ENSMUST00000084141

SMART Domains

Protein: ENSMUSP00000081158
Gene: ENSMUSG00000069743

Domain	Start	End	E-Value	Type
KRAB	13	73	8.77e-20	SMART
ZnF_C2H2	183	205	6.32e-3	SMART
ZnF_C2H2	211	233	2.79e-4	SMART
ZnF_C2H2	239	261	4.3e-5	SMART
ZnF_C2H2	267	289	4.61e-5	SMART
ZnF_C2H2	295	317	1.2e-3	SMART
ZnF_C2H2	323	345	1.22e-4	SMART
ZnF_C2H2	351	373	2.75e-3	SMART
ZnF_C2H2	379	401	2.95e-3	SMART
ZnF_C2H2	407	429	5.29e-5	SMART
ZnF_C2H2	435	457	1.42e-5	SMART
ZnF_C2H2	463	485	1.2e-3	SMART
ZnF_C2H2	491	513	1.13e-4	SMART

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.4%
3x: 99.0%
10x: 97.8%
20x: 96.0%

Validation Efficiency

100% (49/49)

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aasdh	G	A	5: 77,024,130 (GRCm39)	T174M	probably damaging	Het
Adh1	A	T	3: 137,992,558 (GRCm39)	I225F	possibly damaging	Het
Agap2	A	G	10: 126,923,319 (GRCm39)	K691E	unknown	Het
Alcam	T	C	16: 52,073,573 (GRCm39)	D564G	possibly damaging	Het
Apbb2	T	A	5: 66,557,598 (GRCm39)	T289S	probably damaging	Het
Cacna1c	C	T	6: 118,589,586 (GRCm39)	R1446H	probably damaging	Het
Cep68	T	C	11: 20,189,393 (GRCm39)	I540V	probably benign	Het
Ctla4	T	C	1: 60,948,397 (GRCm39)	V23A	probably benign	Het
Dnah7b	T	A	1: 46,379,292 (GRCm39)	D3675E	probably benign	Het
Fam209	G	T	2: 172,314,555 (GRCm39)	V15F	probably benign	Het
Fat3	A	G	9: 15,942,664 (GRCm39)	V1236A	possibly damaging	Het
Gabbr2	A	G	4: 46,718,904 (GRCm39)	I38T	probably damaging	Het
Gdpd4	T	C	7: 97,615,505 (GRCm39)	I110T	probably damaging	Het
Gm4841	C	T	18: 60,402,852 (GRCm39)	A414T	possibly damaging	Het
Gtf3c4	A	T	2: 28,724,782 (GRCm39)	Y176N	probably damaging	Het
H2bc18	G	A	3: 96,177,060 (GRCm39)		probably null	Het
Igsf9b	T	C	9: 27,230,612 (GRCm39)	Y264H	probably damaging	Het
Itih4	A	T	14: 30,614,231 (GRCm39)	N394Y	probably damaging	Het
Kmt2c	T	C	5: 25,500,605 (GRCm39)	T3815A	possibly damaging	Het
Lrwd1	T	C	5: 136,152,254 (GRCm39)		probably null	Het
Mc3r	A	G	2: 172,091,711 (GRCm39)	K311R	probably benign	Het
Myb	T	C	10: 21,032,259 (GRCm39)	T41A	possibly damaging	Het
Nfix	T	C	8: 85,454,554 (GRCm39)	D201G	probably damaging	Het
Nos1	T	C	5: 118,085,512 (GRCm39)	V1280A	probably damaging	Het
Or51a5	A	C	7: 102,771,782 (GRCm39)	S66A	probably benign	Het
Or8a1b	T	C	9: 37,623,168 (GRCm39)	M136V	probably benign	Het
Pcdh15	A	G	10: 74,178,488 (GRCm39)	T582A	probably damaging	Het
Pcdhb12	C	A	18: 37,570,375 (GRCm39)	A507E	probably damaging	Het
Pdia3	G	A	2: 121,262,858 (GRCm39)	G275S	probably damaging	Het
Pkp2	C	A	16: 16,087,575 (GRCm39)	A753E	probably damaging	Het
Plekha3	G	A	2: 76,513,142 (GRCm39)	V76I	possibly damaging	Het
Pnpla6	T	C	8: 3,567,081 (GRCm39)	L33P	probably damaging	Het
Pot1b	C	T	17: 55,973,400 (GRCm39)		probably benign	Het
Prr5l	T	C	2: 101,588,886 (GRCm39)	E88G	possibly damaging	Het
Prss57	G	A	10: 79,621,699 (GRCm39)	H97Y	probably damaging	Het
Rttn	C	T	18: 88,991,813 (GRCm39)	Q131*	probably null	Het
Scaf11	G	T	15: 96,316,176 (GRCm39)	S1129R	possibly damaging	Het
Slc25a20	T	A	9: 108,559,189 (GRCm39)	M185K	possibly damaging	Het
Sorbs3	T	A	14: 70,445,021 (GRCm39)	E19V	probably damaging	Het
Srgap2	A	T	1: 131,217,253 (GRCm39)	C1020S	probably benign	Het
Thoc2l	T	C	5: 104,666,875 (GRCm39)	S466P	probably benign	Het
Tmem109	T	C	19: 10,849,205 (GRCm39)	R217G	possibly damaging	Het
Trio	A	T	15: 27,732,980 (GRCm39)	I2967N	probably damaging	Het
Vars2	T	C	17: 35,968,191 (GRCm39)	E899G	probably benign	Het
Vmn2r109	A	G	17: 20,774,842 (GRCm39)		probably benign	Het
Vps53	A	G	11: 75,973,485 (GRCm39)	F170L	probably damaging	Het
Zc2hc1a	G	A	3: 7,591,422 (GRCm39)	S149N	possibly damaging	Het
Zhx3	A	C	2: 160,622,629 (GRCm39)	F513V	probably damaging	Het

Other mutations in Zfp820

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00424:Zfp820	APN	17	22,038,292 (GRCm39)	missense	probably damaging	1.00
IGL00954:Zfp820	APN	17	22,038,860 (GRCm39)	missense	probably damaging	1.00
IGL01088:Zfp820	APN	17	22,040,162 (GRCm39)	nonsense	probably null
IGL03067:Zfp820	APN	17	22,038,801 (GRCm39)	missense	possibly damaging	0.68
R0158:Zfp820	UTSW	17	22,038,800 (GRCm39)	missense	probably benign	0.06
R0617:Zfp820	UTSW	17	22,038,685 (GRCm39)	missense	probably damaging	0.99
R0658:Zfp820	UTSW	17	22,037,901 (GRCm39)	missense	probably benign	0.00
R0696:Zfp820	UTSW	17	22,039,041 (GRCm39)	missense	possibly damaging	0.94
R0791:Zfp820	UTSW	17	22,038,509 (GRCm39)	missense	probably benign	0.05
R0792:Zfp820	UTSW	17	22,038,509 (GRCm39)	missense	probably benign	0.05
R1421:Zfp820	UTSW	17	22,038,861 (GRCm39)	missense	possibly damaging	0.85
R1573:Zfp820	UTSW	17	22,037,737 (GRCm39)	missense	probably benign	0.00
R1928:Zfp820	UTSW	17	22,038,316 (GRCm39)	missense	probably benign	0.00
R3889:Zfp820	UTSW	17	22,037,877 (GRCm39)	missense	probably benign	0.25
R4782:Zfp820	UTSW	17	22,037,966 (GRCm39)	missense	probably benign	0.00
R4799:Zfp820	UTSW	17	22,037,966 (GRCm39)	missense	probably benign	0.00
R4807:Zfp820	UTSW	17	22,042,853 (GRCm39)	start codon destroyed	probably null	1.00
R4913:Zfp820	UTSW	17	22,038,200 (GRCm39)	missense	probably benign	0.42
R5100:Zfp820	UTSW	17	22,040,054 (GRCm39)	missense	possibly damaging	0.64
R5766:Zfp820	UTSW	17	22,038,983 (GRCm39)	missense	probably damaging	0.96
R5772:Zfp820	UTSW	17	22,037,702 (GRCm39)	missense	probably damaging	1.00
R6516:Zfp820	UTSW	17	22,038,354 (GRCm39)	missense	probably damaging	1.00
R6577:Zfp820	UTSW	17	22,038,384 (GRCm39)	missense	probably benign	0.00
R7094:Zfp820	UTSW	17	22,038,246 (GRCm39)	missense	probably benign	0.41
R7570:Zfp820	UTSW	17	22,037,994 (GRCm39)	missense	probably benign	0.14
R7794:Zfp820	UTSW	17	22,039,109 (GRCm39)	missense	probably damaging	0.98
R8545:Zfp820	UTSW	17	22,038,438 (GRCm39)	missense	probably damaging	1.00
R8692:Zfp820	UTSW	17	22,037,876 (GRCm39)	missense	probably benign	0.43
R9072:Zfp820	UTSW	17	22,039,031 (GRCm39)	missense	possibly damaging	0.91
R9073:Zfp820	UTSW	17	22,039,031 (GRCm39)	missense	possibly damaging	0.91
R9325:Zfp820	UTSW	17	22,038,380 (GRCm39)	missense	probably damaging	1.00
R9421:Zfp820	UTSW	17	22,038,336 (GRCm39)	missense	probably benign	0.20
R9480:Zfp820	UTSW	17	22,037,994 (GRCm39)	missense	possibly damaging	0.85
R9592:Zfp820	UTSW	17	22,038,577 (GRCm39)	missense	probably benign	0.01
R9600:Zfp820	UTSW	17	22,038,861 (GRCm39)	missense	probably benign	0.00
R9632:Zfp820	UTSW	17	22,038,107 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CACAAGGCCGTGCTTGTCTTATTTG -3'
(R):5'- AATGCTCAGCATCCATGTTAGTCCC -3'

Sequencing Primer
(F):5'- CGTGCTTGTCTTATTTGAAGTGAAAG -3'
(R):5'- gagtagcagtgttttacagcag -3'

Posted On

2013-11-07