Incidental Mutation 'R0883:Slit2'
ID 80764
Institutional Source Beutler Lab
Gene Symbol Slit2
Ensembl Gene ENSMUSG00000031558
Gene Name slit guidance ligand 2
Synonyms E030015M03Rik, Drad-1, b2b1200.1Clo, Slil3, E130320P19Rik
MMRRC Submission 039050-MU
Accession Numbers
Essential gene? Essential (E-score: 1.000) question?
Stock # R0883 (G1)
Quality Score 225
Status Validated
Chromosome 5
Chromosomal Location 48140480-48465075 bp(+) (GRCm39)
Type of Mutation splice site
DNA Base Change (assembly) A to G at 48402915 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change
Ref Sequence ENSEMBL: ENSMUSP00000134263 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000033967] [ENSMUST00000170109] [ENSMUST00000172493] [ENSMUST00000173107] [ENSMUST00000174313] [ENSMUST00000174421]
AlphaFold Q9R1B9
Predicted Effect probably benign
Transcript: ENSMUST00000033967
SMART Domains Protein: ENSMUSP00000033967
Gene: ENSMUSG00000031558

DomainStartEndE-ValueType
signal peptide 1 18 N/A INTRINSIC
LRRNT 27 59 6.53e-9 SMART
LRR 53 77 1.41e2 SMART
LRR_TYP 78 101 1.79e-2 SMART
LRR 102 125 2.45e0 SMART
LRR 126 149 9.96e-1 SMART
LRR 154 173 1.29e2 SMART
LRR_TYP 174 197 2.05e-2 SMART
LRRCT 209 258 3.42e-9 SMART
LRRNT 272 304 4.55e-8 SMART
LRR 298 322 3e1 SMART
LRR_TYP 323 346 1.95e-3 SMART
LRR_TYP 347 370 2.24e-3 SMART
LRR 371 394 1.31e0 SMART
LRR 395 418 2.49e-1 SMART
LRRCT 430 479 1.88e-6 SMART
LRRNT 497 529 1.45e-6 SMART
LRR_TYP 549 572 1.38e-3 SMART
LRR 597 620 9.96e-1 SMART
LRR_TYP 621 644 2.71e-2 SMART
LRRCT 656 705 3.56e-7 SMART
LRRNT 718 750 3.69e-8 SMART
LRR 768 791 7.36e0 SMART
LRR_TYP 792 815 5.59e-4 SMART
LRR_TYP 816 839 7.9e-4 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000170109
SMART Domains Protein: ENSMUSP00000127615
Gene: ENSMUSG00000031558

DomainStartEndE-ValueType
signal peptide 1 18 N/A INTRINSIC
LRRNT 27 59 6.53e-9 SMART
LRR 53 77 1.41e2 SMART
LRR_TYP 78 101 1.79e-2 SMART
LRR 102 125 2.45e0 SMART
LRR 126 149 9.96e-1 SMART
LRR 154 173 1.29e2 SMART
LRR_TYP 174 197 2.05e-2 SMART
LRRCT 209 258 3.42e-9 SMART
LRRNT 272 304 4.55e-8 SMART
LRR 298 322 3e1 SMART
LRR_TYP 323 346 1.95e-3 SMART
LRR_TYP 347 370 2.24e-3 SMART
LRR 371 394 1.31e0 SMART
LRR 395 418 2.49e-1 SMART
LRRCT 430 479 1.88e-6 SMART
LRRNT 497 529 1.45e-6 SMART
LRR_TYP 549 572 1.38e-3 SMART
LRR 597 620 9.96e-1 SMART
LRR_TYP 621 644 2.71e-2 SMART
LRRCT 656 705 3.56e-7 SMART
LRRNT 718 750 3.69e-8 SMART
LRR 768 791 7.36e0 SMART
LRR_TYP 792 815 5.59e-4 SMART
LRR_TYP 816 839 7.9e-4 SMART
LRRCT 851 900 3.9e-13 SMART
EGF 913 947 3.73e-5 SMART
EGF 952 988 4.35e-6 SMART
EGF_CA 990 1026 2.21e-7 SMART
FOLN 993 1015 5.84e1 SMART
EGF 1031 1066 1.07e-5 SMART
EGF_CA 1068 1104 3.97e-9 SMART
FOLN 1116 1138 2.22e0 SMART
EGF 1116 1149 1.62e-5 SMART
LamG 1172 1308 4.82e-39 SMART
EGF 1327 1360 3.68e-4 SMART
EGF 1366 1399 3.88e-3 SMART
FOLN 1407 1429 3.34e0 SMART
EGF 1407 1440 4.46e-3 SMART
CT 1451 1520 4.23e-4 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000172484
Predicted Effect probably benign
Transcript: ENSMUST00000172493
SMART Domains Protein: ENSMUSP00000134655
Gene: ENSMUSG00000031558

DomainStartEndE-ValueType
LRR 20 43 3.1e-2 SMART
LRR_TYP 44 67 2.4e-6 SMART
LRR_TYP 68 91 3.2e-6 SMART
LRRCT 103 152 1.8e-15 SMART
EGF 176 210 1.8e-7 SMART
EGF 215 251 2.1e-8 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000173107
SMART Domains Protein: ENSMUSP00000133840
Gene: ENSMUSG00000031558

DomainStartEndE-ValueType
signal peptide 1 18 N/A INTRINSIC
LRRNT 27 59 6.53e-9 SMART
LRR 53 77 1.41e2 SMART
LRR_TYP 78 101 1.79e-2 SMART
LRR 102 125 2.45e0 SMART
LRR 126 149 9.96e-1 SMART
LRR 154 173 1.29e2 SMART
LRR_TYP 174 197 2.05e-2 SMART
LRRCT 209 258 3.42e-9 SMART
LRRNT 272 304 4.55e-8 SMART
LRR 298 322 3e1 SMART
LRR_TYP 323 346 1.95e-3 SMART
LRR_TYP 347 370 2.24e-3 SMART
LRR 371 394 1.31e0 SMART
LRR 395 418 2.49e-1 SMART
LRRCT 430 479 1.88e-6 SMART
LRRNT 505 537 1.45e-6 SMART
LRR_TYP 557 580 1.38e-3 SMART
LRR 605 628 9.96e-1 SMART
LRR_TYP 629 652 2.71e-2 SMART
LRRCT 664 713 3.56e-7 SMART
LRRNT 726 758 3.69e-8 SMART
LRR 776 799 7.36e0 SMART
LRR_TYP 800 823 5.59e-4 SMART
LRR_TYP 824 847 7.9e-4 SMART
LRRCT 859 908 3.9e-13 SMART
EGF 921 955 3.73e-5 SMART
EGF 960 996 4.35e-6 SMART
EGF_CA 998 1034 2.21e-7 SMART
FOLN 1001 1023 5.84e1 SMART
EGF 1039 1074 1.07e-5 SMART
EGF_CA 1076 1112 3.97e-9 SMART
FOLN 1124 1146 2.22e0 SMART
EGF 1124 1157 1.62e-5 SMART
LamG 1180 1316 4.82e-39 SMART
EGF 1335 1368 3.68e-4 SMART
EGF 1374 1407 3.88e-3 SMART
FOLN 1415 1437 3.34e0 SMART
EGF 1415 1448 4.46e-3 SMART
CT 1459 1528 4.23e-4 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000173671
Predicted Effect probably benign
Transcript: ENSMUST00000174313
SMART Domains Protein: ENSMUSP00000133912
Gene: ENSMUSG00000031558

DomainStartEndE-ValueType
signal peptide 1 18 N/A INTRINSIC
LRRNT 27 59 6.53e-9 SMART
LRR 53 77 1.41e2 SMART
LRR_TYP 78 101 1.79e-2 SMART
LRR 102 125 2.45e0 SMART
LRR 126 149 9.96e-1 SMART
LRR 154 173 1.29e2 SMART
LRR_TYP 174 197 2.05e-2 SMART
LRRCT 209 258 3.42e-9 SMART
LRRNT 276 308 4.55e-8 SMART
LRR 302 326 3e1 SMART
LRR_TYP 327 350 1.95e-3 SMART
LRR_TYP 351 374 2.24e-3 SMART
LRR 375 398 1.31e0 SMART
LRR 399 422 2.49e-1 SMART
LRRCT 434 483 1.88e-6 SMART
LRRNT 501 533 1.45e-6 SMART
LRR_TYP 553 576 1.38e-3 SMART
LRR 601 624 9.96e-1 SMART
LRR_TYP 625 648 2.71e-2 SMART
LRRCT 660 709 3.56e-7 SMART
LRRNT 722 754 3.69e-8 SMART
LRR 772 795 7.36e0 SMART
LRR_TYP 796 819 5.59e-4 SMART
LRR_TYP 820 843 7.9e-4 SMART
LRRCT 855 904 3.9e-13 SMART
EGF 917 951 3.73e-5 SMART
EGF 956 992 4.35e-6 SMART
EGF_CA 994 1030 2.21e-7 SMART
FOLN 997 1019 5.84e1 SMART
EGF 1035 1070 1.07e-5 SMART
EGF_CA 1072 1108 3.97e-9 SMART
FOLN 1120 1142 2.22e0 SMART
EGF 1120 1153 1.62e-5 SMART
LamG 1176 1312 4.82e-39 SMART
EGF 1331 1364 3.68e-4 SMART
EGF 1370 1403 3.88e-3 SMART
FOLN 1411 1433 3.34e0 SMART
EGF 1411 1444 4.46e-3 SMART
CT 1455 1524 4.23e-4 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000174421
SMART Domains Protein: ENSMUSP00000134263
Gene: ENSMUSG00000031558

DomainStartEndE-ValueType
signal peptide 1 18 N/A INTRINSIC
LRRNT 27 59 6.53e-9 SMART
LRR 53 77 1.41e2 SMART
LRR_TYP 78 101 1.79e-2 SMART
LRR 102 125 2.45e0 SMART
LRR 126 149 9.96e-1 SMART
LRR 154 173 1.29e2 SMART
LRR_TYP 174 197 2.05e-2 SMART
LRRCT 209 258 3.42e-9 SMART
LRRNT 276 308 4.55e-8 SMART
LRR 302 326 3e1 SMART
LRR_TYP 327 350 1.95e-3 SMART
LRR_TYP 351 374 2.24e-3 SMART
LRR 375 398 1.31e0 SMART
LRR 399 422 2.49e-1 SMART
LRRCT 434 483 1.88e-6 SMART
LRRNT 509 541 1.45e-6 SMART
LRR_TYP 561 584 1.38e-3 SMART
LRR 609 632 9.96e-1 SMART
LRR_TYP 633 656 2.71e-2 SMART
LRRCT 668 717 3.56e-7 SMART
LRRNT 730 762 3.69e-8 SMART
LRR 780 803 7.36e0 SMART
LRR_TYP 804 827 5.59e-4 SMART
LRR_TYP 828 851 7.9e-4 SMART
LRRCT 863 912 3.9e-13 SMART
EGF 925 959 3.73e-5 SMART
EGF 964 1000 4.35e-6 SMART
EGF_CA 1002 1047 4.74e-7 SMART
FOLN 1005 1027 5.84e1 SMART
EGF 1052 1087 1.07e-5 SMART
EGF_CA 1089 1125 3.97e-9 SMART
FOLN 1137 1159 2.22e0 SMART
EGF 1137 1170 1.62e-5 SMART
LamG 1193 1329 4.82e-39 SMART
EGF 1348 1381 3.68e-4 SMART
EGF 1387 1420 3.88e-3 SMART
FOLN 1428 1450 3.34e0 SMART
EGF 1428 1461 4.46e-3 SMART
CT 1472 1541 4.23e-4 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000199024
Coding Region Coverage
  • 1x: 99.5%
  • 3x: 99.0%
  • 10x: 97.8%
  • 20x: 96.1%
Validation Efficiency 98% (148/151)
MGI Phenotype FUNCTION: The protein encoded by this gene is a member of the Slit family of secreted glycoproteins, which function as ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. In mammals, members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Mice deficient for this gene exhibit abnormal axonal projections in the embryonic forebrain and develop supernumerary uretic buds that maintain improper connections to the nephric duct. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
PHENOTYPE: Homozygous mutants display perinatal lethality, abnormal ureteric bud development, multiple fused kidneys, multiple ureters, and hydroureter. [provided by MGI curators]
Allele List at MGI

All alleles(4) : Targeted(4)
Other mutations in this stock
Total: 150 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1110002E22Rik T C 3: 137,775,632 (GRCm39) L1607P probably damaging Het
1700016H13Rik T C 5: 103,796,687 (GRCm39) *118W probably null Het
4930595M18Rik G T X: 80,464,537 (GRCm39) T390N possibly damaging Het
Abca13 C T 11: 9,241,238 (GRCm39) Q1034* probably null Het
Acsbg3 A T 17: 57,190,835 (GRCm39) N468Y probably benign Het
Adgra3 T C 5: 50,118,065 (GRCm39) H1161R probably damaging Het
Aff1 T C 5: 103,974,004 (GRCm39) probably benign Het
Agap2 A G 10: 126,927,571 (GRCm39) T1131A possibly damaging Het
Ankrd12 A G 17: 66,292,127 (GRCm39) V1102A probably benign Het
Ankrd54 A T 15: 78,946,931 (GRCm39) C23S probably damaging Het
Anxa10 C T 8: 62,531,001 (GRCm39) V70I probably benign Het
Asap3 T C 4: 135,961,636 (GRCm39) probably benign Het
Asb13 T C 13: 3,695,052 (GRCm39) probably null Het
Atp6v1a A T 16: 43,922,055 (GRCm39) probably benign Het
Atp8b1 T G 18: 64,697,612 (GRCm39) I411L probably benign Het
Baiap3 T A 17: 25,468,075 (GRCm39) N313I probably damaging Het
Bok T C 1: 93,614,209 (GRCm39) I14T probably benign Het
Bri3bp T A 5: 125,518,808 (GRCm39) probably null Het
C2cd2l A G 9: 44,227,499 (GRCm39) L186P probably damaging Het
Cadm2 A T 16: 66,679,702 (GRCm39) C44S probably damaging Het
Capn11 T C 17: 45,949,807 (GRCm39) probably benign Het
Carm1 T A 9: 21,480,887 (GRCm39) probably benign Het
Ccdc27 T C 4: 154,120,941 (GRCm39) E285G unknown Het
Cct3 T A 3: 88,220,864 (GRCm39) D298E probably damaging Het
Cd59b T A 2: 103,911,331 (GRCm39) probably benign Het
Cdh2 T C 18: 16,762,633 (GRCm39) N437S probably benign Het
Celsr3 T A 9: 108,719,832 (GRCm39) I2470N probably damaging Het
Cfap100 G A 6: 90,392,888 (GRCm39) probably benign Het
Cfap119 T C 7: 127,184,034 (GRCm39) E261G probably damaging Het
Cfap45 A T 1: 172,359,756 (GRCm39) R98S possibly damaging Het
Cfap54 T A 10: 92,706,531 (GRCm39) H2757L unknown Het
Chd1 C A 17: 15,945,693 (GRCm39) N72K probably benign Het
Cntn4 A T 6: 106,644,501 (GRCm39) probably benign Het
Cstf2t A T 19: 31,062,026 (GRCm39) M521L probably benign Het
Daam2 A T 17: 49,805,911 (GRCm39) probably benign Het
Ddias A T 7: 92,508,545 (GRCm39) W457R probably benign Het
Ddr2 C T 1: 169,822,198 (GRCm39) V417I probably benign Het
Dhx57 T C 17: 80,577,800 (GRCm39) T570A probably damaging Het
Dmp1 A G 5: 104,355,496 (GRCm39) E32G possibly damaging Het
Dtymk C T 1: 93,729,510 (GRCm39) V14M possibly damaging Het
Dync2li1 G A 17: 84,956,699 (GRCm39) M286I probably benign Het
Eea1 G A 10: 95,857,529 (GRCm39) D664N possibly damaging Het
Esp6 G T 17: 40,876,287 (GRCm39) V112L probably benign Het
Fam83h G T 15: 75,878,018 (GRCm39) Q127K probably damaging Het
Gabbr2 G A 4: 46,677,474 (GRCm39) T802I probably benign Het
Gart C A 16: 91,420,291 (GRCm39) D851Y possibly damaging Het
Gemin6 T A 17: 80,535,524 (GRCm39) H161Q probably damaging Het
Gm10912 T C 2: 103,896,875 (GRCm39) S5P probably benign Het
Gm5941 G A X: 91,533,817 (GRCm39) A62T possibly damaging Het
Gng2 G T 14: 19,941,363 (GRCm39) D26E probably benign Het
Gpr33 A G 12: 52,070,418 (GRCm39) V207A probably benign Het
Gstm3 T A 3: 107,873,586 (GRCm39) probably benign Het
Havcr1 T C 11: 46,643,259 (GRCm39) C60R probably damaging Het
Hspg2 T C 4: 137,268,751 (GRCm39) S2157P probably benign Het
Ift140 A G 17: 25,309,907 (GRCm39) T1105A probably benign Het
Igsf8 C A 1: 172,143,826 (GRCm39) A56D possibly damaging Het
Kat6a G T 8: 23,352,230 (GRCm39) A5S probably damaging Het
Kctd16 A G 18: 40,663,828 (GRCm39) E319G probably damaging Het
Kmo T C 1: 175,474,706 (GRCm39) V157A possibly damaging Het
Lrp5 T A 19: 3,655,308 (GRCm39) I1071F probably damaging Het
Lrrc17 A G 5: 21,766,276 (GRCm39) T253A probably benign Het
Mast2 T A 4: 116,168,964 (GRCm39) H769L probably damaging Het
Mast4 T C 13: 102,990,408 (GRCm39) K50E probably damaging Het
Mbd5 A G 2: 49,146,701 (GRCm39) T304A possibly damaging Het
Mbp T C 18: 82,590,995 (GRCm39) S73P probably damaging Het
Mc5r T A 18: 68,472,163 (GRCm39) V174E probably damaging Het
Med13 T A 11: 86,197,864 (GRCm39) T736S probably benign Het
Med13l T C 5: 118,809,067 (GRCm39) probably benign Het
Minar1 T C 9: 89,484,470 (GRCm39) H309R probably benign Het
Mlh3 C G 12: 85,282,488 (GRCm39) A1382P possibly damaging Het
Mpdz T C 4: 81,278,228 (GRCm39) probably benign Het
Muc5ac A G 7: 141,350,002 (GRCm39) T582A possibly damaging Het
Nalcn A G 14: 123,702,152 (GRCm39) F453S probably damaging Het
Nrap T A 19: 56,333,906 (GRCm39) M902L probably damaging Het
Nup85 C T 11: 115,459,196 (GRCm39) R100* probably null Het
Nxf1 T G 19: 8,741,955 (GRCm39) N296K probably damaging Het
Ogg1 A G 6: 113,305,381 (GRCm39) T65A probably damaging Het
Ogt A G X: 100,687,805 (GRCm39) probably benign Het
Or4c10 A G 2: 89,760,545 (GRCm39) T131A probably benign Het
Or4k48 C T 2: 111,476,136 (GRCm39) V69I probably benign Het
Or51e1 T A 7: 102,359,202 (GRCm39) H245Q probably damaging Het
Or56b1b T A 7: 108,164,483 (GRCm39) N173I probably benign Het
Ovol2 T C 2: 144,173,710 (GRCm39) D24G probably damaging Het
Pabpc1 A G 15: 36,599,298 (GRCm39) probably benign Het
Pak6 T C 2: 118,524,168 (GRCm39) L441P probably damaging Het
Pappa T A 4: 65,107,552 (GRCm39) C654* probably null Het
Paqr6 T A 3: 88,273,298 (GRCm39) S97T probably damaging Het
Parp14 T C 16: 35,678,888 (GRCm39) N360S probably benign Het
Pclo G T 5: 14,727,873 (GRCm39) G2244* probably null Het
Pdzrn3 A T 6: 101,132,903 (GRCm39) probably null Het
Pes1 T C 11: 3,925,557 (GRCm39) M220T probably damaging Het
Phip A T 9: 82,758,274 (GRCm39) V1473E probably benign Het
Pkd2l2 T A 18: 34,563,321 (GRCm39) probably null Het
Plch1 T C 3: 63,660,677 (GRCm39) D302G probably damaging Het
Plekhh2 A G 17: 84,925,459 (GRCm39) T1419A probably benign Het
Ppara A T 15: 85,682,372 (GRCm39) E356V probably damaging Het
Ppp1r37 G A 7: 19,266,102 (GRCm39) P555S probably benign Het
Ppp6r1 T C 7: 4,642,709 (GRCm39) E545G possibly damaging Het
Proser3 G A 7: 30,240,124 (GRCm39) H327Y probably damaging Het
Prss43 T A 9: 110,658,576 (GRCm39) I292N probably damaging Het
Pwwp3b T A X: 138,136,444 (GRCm39) D327E probably damaging Het
Pygl G C 12: 70,253,178 (GRCm39) N271K probably damaging Het
Rassf7 T A 7: 140,796,903 (GRCm39) probably benign Het
Rfx2 T C 17: 57,110,722 (GRCm39) Y88C probably damaging Het
Rpl6 T C 5: 121,346,541 (GRCm39) V214A probably benign Het
Rspo1 T A 4: 124,885,225 (GRCm39) probably null Het
Sav1 A C 12: 70,012,979 (GRCm39) L366V probably benign Het
Sema3b T G 9: 107,481,355 (GRCm39) T52P possibly damaging Het
Senp6 A G 9: 80,023,841 (GRCm39) D40G probably damaging Het
Sh3pxd2a A G 19: 47,256,646 (GRCm39) S719P probably damaging Het
Shank1 C T 7: 44,001,718 (GRCm39) R1146W unknown Het
Slc34a3 G T 2: 25,121,245 (GRCm39) D307E probably benign Het
Slc35b3 T C 13: 39,121,251 (GRCm39) I330V probably benign Het
Slc4a10 G A 2: 62,073,742 (GRCm39) C268Y probably benign Het
Slco6d1 A G 1: 98,349,124 (GRCm39) E65G probably benign Het
Smcr8 T C 11: 60,668,941 (GRCm39) Y30H probably damaging Het
Snap47 A G 11: 59,329,326 (GRCm39) probably benign Het
Snrnp25 G A 11: 32,156,960 (GRCm39) V15I probably damaging Het
Spns2 T C 11: 72,345,223 (GRCm39) Y449C probably damaging Het
Stab2 T A 10: 86,760,314 (GRCm39) probably benign Het
Strip1 C A 3: 107,521,929 (GRCm39) D750Y probably damaging Het
Taf1c A T 8: 120,326,722 (GRCm39) I438N probably damaging Het
Tbc1d2 T A 4: 46,609,003 (GRCm39) K745* probably null Het
Tctn1 T C 5: 122,402,207 (GRCm39) T76A probably damaging Het
Tesl1 A T X: 23,773,290 (GRCm39) I264F probably benign Het
Tfpi T C 2: 84,273,664 (GRCm39) probably benign Het
Timm44 A T 8: 4,316,592 (GRCm39) H317Q probably benign Het
Tnfaip1 A T 11: 78,420,840 (GRCm39) probably benign Het
Tnpo3 A T 6: 29,554,992 (GRCm39) probably benign Het
Top3b T C 16: 16,697,301 (GRCm39) probably benign Het
Trak1 T A 9: 121,282,351 (GRCm39) M410K possibly damaging Het
Trpm3 C A 19: 22,956,018 (GRCm39) P1160Q probably damaging Het
Tyk2 T A 9: 21,022,433 (GRCm39) T799S possibly damaging Het
Ubfd1 G A 7: 121,666,714 (GRCm39) probably benign Het
Unc13a G A 8: 72,094,817 (GRCm39) R1272* probably null Het
Unc45b T A 11: 82,831,031 (GRCm39) L797Q possibly damaging Het
Urb2 C T 8: 124,757,709 (GRCm39) Q1139* probably null Het
Vmn2r66 A T 7: 84,657,070 (GRCm39) S112T probably benign Het
Vmn2r71 A T 7: 85,272,842 (GRCm39) D552V probably benign Het
Vmn2r76 A G 7: 85,877,904 (GRCm39) Y498H probably benign Het
Vmn2r84 A C 10: 130,226,984 (GRCm39) W285G probably damaging Het
Vps72 G T 3: 95,029,894 (GRCm39) L304F probably damaging Het
Wiz A T 17: 32,575,415 (GRCm39) I907N probably damaging Het
Yaf2 T C 15: 93,183,417 (GRCm39) K131R probably damaging Het
Zfp141 A T 7: 42,125,480 (GRCm39) Y331N possibly damaging Het
Zfp324 G T 7: 12,704,951 (GRCm39) C380F probably damaging Het
Zfp521 T C 18: 13,978,119 (GRCm39) T765A probably benign Het
Zfp616 A T 11: 73,976,500 (GRCm39) H923L probably damaging Het
Zfpm1 C T 8: 123,062,585 (GRCm39) T548M probably damaging Het
Zp2 A T 7: 119,742,799 (GRCm39) probably benign Het
Other mutations in Slit2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00791:Slit2 APN 5 48,461,374 (GRCm39) missense possibly damaging 0.86
IGL00809:Slit2 APN 5 48,146,493 (GRCm39) missense possibly damaging 0.88
IGL00811:Slit2 APN 5 48,146,493 (GRCm39) missense possibly damaging 0.88
IGL00813:Slit2 APN 5 48,146,493 (GRCm39) missense possibly damaging 0.88
IGL00815:Slit2 APN 5 48,146,493 (GRCm39) missense possibly damaging 0.88
IGL00816:Slit2 APN 5 48,146,493 (GRCm39) missense possibly damaging 0.88
IGL00817:Slit2 APN 5 48,146,493 (GRCm39) missense possibly damaging 0.88
IGL00819:Slit2 APN 5 48,146,493 (GRCm39) missense possibly damaging 0.88
IGL00820:Slit2 APN 5 48,146,493 (GRCm39) missense possibly damaging 0.88
IGL00822:Slit2 APN 5 48,146,493 (GRCm39) missense possibly damaging 0.88
IGL01077:Slit2 APN 5 48,374,785 (GRCm39) splice site probably null
IGL01375:Slit2 APN 5 48,439,056 (GRCm39) splice site probably benign
IGL01481:Slit2 APN 5 48,460,273 (GRCm39) missense probably benign 0.05
IGL01934:Slit2 APN 5 48,395,747 (GRCm39) missense possibly damaging 0.93
IGL01992:Slit2 APN 5 48,395,759 (GRCm39) missense probably benign 0.01
IGL02315:Slit2 APN 5 48,145,213 (GRCm39) missense probably damaging 0.98
IGL02328:Slit2 APN 5 48,387,646 (GRCm39) missense probably damaging 1.00
IGL02366:Slit2 APN 5 48,461,410 (GRCm39) missense possibly damaging 0.53
IGL02526:Slit2 APN 5 48,461,565 (GRCm39) nonsense probably null
IGL02852:Slit2 APN 5 48,402,014 (GRCm39) missense probably damaging 1.00
IGL02887:Slit2 APN 5 48,374,816 (GRCm39) missense probably benign 0.44
IGL03123:Slit2 APN 5 48,368,681 (GRCm39) missense probably damaging 1.00
IGL03182:Slit2 APN 5 48,377,395 (GRCm39) missense possibly damaging 0.77
P0025:Slit2 UTSW 5 48,461,377 (GRCm39) missense probably damaging 0.96
R0032:Slit2 UTSW 5 48,414,198 (GRCm39) missense probably damaging 0.99
R0032:Slit2 UTSW 5 48,414,198 (GRCm39) missense probably damaging 0.99
R0055:Slit2 UTSW 5 48,439,068 (GRCm39) nonsense probably null
R0055:Slit2 UTSW 5 48,439,068 (GRCm39) nonsense probably null
R0267:Slit2 UTSW 5 48,339,673 (GRCm39) splice site probably benign
R0552:Slit2 UTSW 5 48,395,721 (GRCm39) missense probably damaging 1.00
R0610:Slit2 UTSW 5 48,433,016 (GRCm39) missense possibly damaging 0.77
R1390:Slit2 UTSW 5 48,374,832 (GRCm39) missense probably benign 0.06
R1442:Slit2 UTSW 5 48,395,725 (GRCm39) missense probably damaging 0.96
R1453:Slit2 UTSW 5 48,414,393 (GRCm39) missense possibly damaging 0.88
R1508:Slit2 UTSW 5 48,349,591 (GRCm39) missense probably damaging 0.98
R1639:Slit2 UTSW 5 48,416,996 (GRCm39) missense probably damaging 1.00
R1705:Slit2 UTSW 5 48,346,814 (GRCm39) missense probably damaging 0.99
R1828:Slit2 UTSW 5 48,461,372 (GRCm39) missense probably damaging 1.00
R1897:Slit2 UTSW 5 48,395,765 (GRCm39) missense probably damaging 1.00
R1908:Slit2 UTSW 5 48,439,330 (GRCm39) missense probably damaging 1.00
R1919:Slit2 UTSW 5 48,348,358 (GRCm39) unclassified probably benign
R1982:Slit2 UTSW 5 48,407,178 (GRCm39) missense probably damaging 1.00
R2013:Slit2 UTSW 5 48,459,832 (GRCm39) missense probably damaging 1.00
R2136:Slit2 UTSW 5 48,461,567 (GRCm39) missense probably benign 0.03
R2655:Slit2 UTSW 5 48,346,917 (GRCm39) missense possibly damaging 0.88
R3402:Slit2 UTSW 5 48,440,763 (GRCm39) missense probably damaging 0.98
R3724:Slit2 UTSW 5 48,414,225 (GRCm39) critical splice donor site probably null
R4176:Slit2 UTSW 5 48,394,586 (GRCm39) splice site probably null
R4306:Slit2 UTSW 5 48,460,125 (GRCm39) missense possibly damaging 0.83
R4397:Slit2 UTSW 5 48,377,423 (GRCm39) critical splice donor site probably null
R4525:Slit2 UTSW 5 48,407,215 (GRCm39) missense probably damaging 1.00
R4688:Slit2 UTSW 5 48,414,345 (GRCm39) splice site probably null
R5026:Slit2 UTSW 5 48,414,147 (GRCm39) missense probably damaging 0.99
R5138:Slit2 UTSW 5 48,439,309 (GRCm39) missense probably damaging 1.00
R5465:Slit2 UTSW 5 48,407,254 (GRCm39) missense probably damaging 1.00
R5471:Slit2 UTSW 5 48,346,897 (GRCm39) missense probably damaging 1.00
R5699:Slit2 UTSW 5 48,378,333 (GRCm39) critical splice donor site probably null
R5735:Slit2 UTSW 5 48,416,958 (GRCm39) missense probably damaging 1.00
R5834:Slit2 UTSW 5 48,416,989 (GRCm39) missense probably damaging 1.00
R5967:Slit2 UTSW 5 48,142,506 (GRCm39) missense probably damaging 0.99
R6150:Slit2 UTSW 5 48,461,516 (GRCm39) missense probably damaging 1.00
R6219:Slit2 UTSW 5 48,459,770 (GRCm39) missense possibly damaging 0.53
R6344:Slit2 UTSW 5 48,377,023 (GRCm39) missense probably benign 0.07
R6408:Slit2 UTSW 5 48,142,328 (GRCm39) unclassified probably benign
R6479:Slit2 UTSW 5 48,389,331 (GRCm39) missense probably damaging 1.00
R6526:Slit2 UTSW 5 48,461,509 (GRCm39) missense probably damaging 0.99
R6959:Slit2 UTSW 5 48,395,727 (GRCm39) missense possibly damaging 0.83
R7139:Slit2 UTSW 5 48,402,025 (GRCm39) missense probably benign 0.19
R7201:Slit2 UTSW 5 48,394,627 (GRCm39) missense probably null 0.85
R7472:Slit2 UTSW 5 48,414,180 (GRCm39) missense probably damaging 0.97
R7491:Slit2 UTSW 5 48,377,336 (GRCm39) missense probably benign 0.18
R7566:Slit2 UTSW 5 48,407,239 (GRCm39) missense probably damaging 0.99
R7622:Slit2 UTSW 5 48,142,547 (GRCm39) missense probably damaging 0.98
R7831:Slit2 UTSW 5 48,402,025 (GRCm39) missense probably benign 0.19
R7870:Slit2 UTSW 5 48,459,649 (GRCm39) missense probably damaging 0.99
R7899:Slit2 UTSW 5 48,404,527 (GRCm39) missense possibly damaging 0.89
R7969:Slit2 UTSW 5 48,461,378 (GRCm39) missense possibly damaging 0.47
R7984:Slit2 UTSW 5 48,333,465 (GRCm39) intron probably benign
R8021:Slit2 UTSW 5 48,459,834 (GRCm39) nonsense probably null
R8253:Slit2 UTSW 5 48,433,013 (GRCm39) missense probably benign 0.00
R8321:Slit2 UTSW 5 48,387,609 (GRCm39) missense probably damaging 1.00
R8426:Slit2 UTSW 5 48,382,105 (GRCm39) missense probably benign 0.00
R8513:Slit2 UTSW 5 48,382,050 (GRCm39) nonsense probably null
R8756:Slit2 UTSW 5 48,459,829 (GRCm39) nonsense probably null
R8796:Slit2 UTSW 5 48,460,190 (GRCm39) missense probably benign 0.01
R8799:Slit2 UTSW 5 48,461,524 (GRCm39) missense possibly damaging 0.73
R8947:Slit2 UTSW 5 48,407,140 (GRCm39) missense probably damaging 1.00
R9005:Slit2 UTSW 5 48,459,860 (GRCm39) missense possibly damaging 0.73
R9173:Slit2 UTSW 5 48,377,285 (GRCm39) missense probably damaging 0.98
R9310:Slit2 UTSW 5 48,349,568 (GRCm39) missense possibly damaging 0.59
R9365:Slit2 UTSW 5 48,461,534 (GRCm39) missense probably benign 0.04
Z1088:Slit2 UTSW 5 48,459,695 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- CCCAGTCAGAGGATGAATGAGGCA -3'
(R):5'- TTGAGAAACAGCTACACACGGTAAAAGA -3'

Sequencing Primer
(F):5'- GAATGAGGCATGTGTACACAGT -3'
(R):5'- CAGTAACATGTATTACACAAGAGCAG -3'
Posted On 2013-11-07