Mutagenetix > Incidental Mutation

Incidental Mutation 'R0883:Nalcn'

80910

Institutional Source

Beutler Lab

Gene Symbol

Nalcn

Ensembl Gene

ENSMUSG00000000197

Gene Name

sodium leak channel, non-selective

Synonyms

A530023G15Rik, Vgcnl1

MMRRC Submission

039050-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R0883 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

123276634-123627144 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 123464740 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Serine at position 453 (F453S)

Ref Sequence

ENSEMBL: ENSMUSP00000000201 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000000201]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000000201
AA Change: F453S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000000201
Gene: ENSMUSG00000000197
AA Change: F453S

Domain	Start	End	E-Value	Type
Pfam:Ion_trans	35	333	2.8e-37	PFAM
low complexity region	338	348	N/A	INTRINSIC
Pfam:Ion_trans	383	609	5.7e-34	PFAM
coiled coil region	796	830	N/A	INTRINSIC
Pfam:Ion_trans	885	1166	2.4e-42	PFAM
Pfam:Ion_trans	1209	1458	6.9e-30	PFAM
low complexity region	1548	1560	N/A	INTRINSIC
low complexity region	1634	1649	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000227818

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000228860

Meta Mutation Damage Score

0.9622

Coding Region Coverage

1x: 99.5%
3x: 99.0%
10x: 97.8%
20x: 96.1%

Validation Efficiency

98% (148/151)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] NALCN forms a voltage-independent, nonselective, noninactivating cation channel permeable to Na+, K+, and Ca(2+). It is responsible for the neuronal background sodium leak conductance (Lu et al., 2007 [PubMed 17448995]).[supplied by OMIM, Mar 2008]
PHENOTYPE: Mice homozygous for a null allele exhibit abnormal breathing at birth and die within 24 hours. Mice homozygous for a gain of function ENU mutation exhibit reduced the total amount and episode duration of REMS. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 150 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	T	C	3: 138,069,871	L1607P	probably damaging	Het
1700016H13Rik	T	C	5: 103,648,821	*118W	probably null	Het
1700061G19Rik	A	T	17: 56,883,835	N468Y	probably benign	Het
4930595M18Rik	G	T	X: 81,420,931	T390N	possibly damaging	Het
Abca13	C	T	11: 9,291,238	Q1034*	probably null	Het
Adgra3	T	C	5: 49,960,723	H1161R	probably damaging	Het
AF529169	T	C	9: 89,602,417	H309R	probably benign	Het
Aff1	T	C	5: 103,826,138		probably benign	Het
Agap2	A	G	10: 127,091,702	T1131A	possibly damaging	Het
Ankrd12	A	G	17: 65,985,132	V1102A	probably benign	Het
Ankrd54	A	T	15: 79,062,731	C23S	probably damaging	Het
Anxa10	C	T	8: 62,077,967	V70I	probably benign	Het
Asap3	T	C	4: 136,234,325		probably benign	Het
Asb13	T	C	13: 3,645,052		probably null	Het
Atp6v1a	A	T	16: 44,101,692		probably benign	Het
Atp8b1	T	G	18: 64,564,541	I411L	probably benign	Het
Baiap3	T	A	17: 25,249,101	N313I	probably damaging	Het
Bok	T	C	1: 93,686,487	I14T	probably benign	Het
Bri3bp	T	A	5: 125,441,744		probably null	Het
C2cd2l	A	G	9: 44,316,202	L186P	probably damaging	Het
Cadm2	A	T	16: 66,882,814	C44S	probably damaging	Het
Capn11	T	C	17: 45,638,881		probably benign	Het
Carm1	T	A	9: 21,569,591		probably benign	Het
Ccdc189	T	C	7: 127,584,862	E261G	probably damaging	Het
Ccdc27	T	C	4: 154,036,484	E285G	unknown	Het
Cct3	T	A	3: 88,313,557	D298E	probably damaging	Het
Cd59b	T	A	2: 104,080,986		probably benign	Het
Cdh2	T	C	18: 16,629,576	N437S	probably benign	Het
Celsr3	T	A	9: 108,842,633	I2470N	probably damaging	Het
Cfap100	G	A	6: 90,415,906		probably benign	Het
Cfap45	A	T	1: 172,532,189	R98S	possibly damaging	Het
Cfap54	T	A	10: 92,870,669	H2757L	unknown	Het
Chd1	C	A	17: 15,725,431	N72K	probably benign	Het
Cntn4	A	T	6: 106,667,540		probably benign	Het
Cstf2t	A	T	19: 31,084,626	M521L	probably benign	Het
Daam2	A	T	17: 49,498,883		probably benign	Het
Ddias	A	T	7: 92,859,337	W457R	probably benign	Het
Ddr2	C	T	1: 169,994,629	V417I	probably benign	Het
Dhx57	T	C	17: 80,270,371	T570A	probably damaging	Het
Dmp1	A	G	5: 104,207,630	E32G	possibly damaging	Het
Dtymk	C	T	1: 93,801,788	V14M	possibly damaging	Het
Dync2li1	G	A	17: 84,649,271	M286I	probably benign	Het
Eea1	G	A	10: 96,021,667	D664N	possibly damaging	Het
Esp6	G	T	17: 40,565,396	V112L	probably benign	Het
Fam83h	G	T	15: 76,006,169	Q127K	probably damaging	Het
Gabbr2	G	A	4: 46,677,474	T802I	probably benign	Het
Gart	C	A	16: 91,623,403	D851Y	possibly damaging	Het
Gemin6	T	A	17: 80,228,095	H161Q	probably damaging	Het
Gm10912	T	C	2: 104,066,530	S5P	probably benign	Het
Gm4907	A	T	X: 23,907,051	I264F	probably benign	Het
Gm5941	G	A	X: 92,490,211	A62T	possibly damaging	Het
Gng2	G	T	14: 19,891,295	D26E	probably benign	Het
Gpr33	A	G	12: 52,023,635	V207A	probably benign	Het
Gstm3	T	A	3: 107,966,270		probably benign	Het
Havcr1	T	C	11: 46,752,432	C60R	probably damaging	Het
Hspg2	T	C	4: 137,541,440	S2157P	probably benign	Het
Ift140	A	G	17: 25,090,933	T1105A	probably benign	Het
Igsf8	C	A	1: 172,316,259	A56D	possibly damaging	Het
Kat6a	G	T	8: 22,862,214	A5S	probably damaging	Het
Kctd16	A	G	18: 40,530,775	E319G	probably damaging	Het
Kmo	T	C	1: 175,647,140	V157A	possibly damaging	Het
Lrp5	T	A	19: 3,605,308	I1071F	probably damaging	Het
Lrrc17	A	G	5: 21,561,278	T253A	probably benign	Het
Mast2	T	A	4: 116,311,767	H769L	probably damaging	Het
Mast4	T	C	13: 102,853,900	K50E	probably damaging	Het
Mbd5	A	G	2: 49,256,689	T304A	possibly damaging	Het
Mbp	T	C	18: 82,572,870	S73P	probably damaging	Het
Mc5r	T	A	18: 68,339,092	V174E	probably damaging	Het
Med13	T	A	11: 86,307,038	T736S	probably benign	Het
Med13l	T	C	5: 118,671,002		probably benign	Het
Mlh3	C	G	12: 85,235,714	A1382P	possibly damaging	Het
Mpdz	T	C	4: 81,359,991		probably benign	Het
Muc5ac	A	G	7: 141,796,265	T582A	possibly damaging	Het
Mum1l1	T	A	X: 139,235,695	D327E	probably damaging	Het
Nrap	T	A	19: 56,345,474	M902L	probably damaging	Het
Nup85	C	T	11: 115,568,370	R100*	probably null	Het
Nxf1	T	G	19: 8,764,591	N296K	probably damaging	Het
Ogg1	A	G	6: 113,328,420	T65A	probably damaging	Het
Ogt	A	G	X: 101,644,199		probably benign	Het
Olfr1258	A	G	2: 89,930,201	T131A	probably benign	Het
Olfr1298	C	T	2: 111,645,791	V69I	probably benign	Het
Olfr504	T	A	7: 108,565,276	N173I	probably benign	Het
Olfr558	T	A	7: 102,709,995	H245Q	probably damaging	Het
Ovol2	T	C	2: 144,331,790	D24G	probably damaging	Het
Pabpc1	A	G	15: 36,599,054		probably benign	Het
Pak6	T	C	2: 118,693,687	L441P	probably damaging	Het
Pappa	T	A	4: 65,189,315	C654*	probably null	Het
Paqr6	T	A	3: 88,365,991	S97T	probably damaging	Het
Parp14	T	C	16: 35,858,518	N360S	probably benign	Het
Pclo	G	T	5: 14,677,859	G2244*	probably null	Het
Pdzrn3	A	T	6: 101,155,942		probably null	Het
Pes1	T	C	11: 3,975,557	M220T	probably damaging	Het
Phip	A	T	9: 82,876,221	V1473E	probably benign	Het
Pkd2l2	T	A	18: 34,430,268		probably null	Het
Plch1	T	C	3: 63,753,256	D302G	probably damaging	Het
Plekhh2	A	G	17: 84,618,031	T1419A	probably benign	Het
Ppara	A	T	15: 85,798,171	E356V	probably damaging	Het
Ppp1r37	G	A	7: 19,532,177	P555S	probably benign	Het
Ppp6r1	T	C	7: 4,639,710	E545G	possibly damaging	Het
Proser3	G	A	7: 30,540,699	H327Y	probably damaging	Het
Prss43	T	A	9: 110,829,508	I292N	probably damaging	Het
Pygl	G	C	12: 70,206,404	N271K	probably damaging	Het
Rassf7	T	A	7: 141,216,990		probably benign	Het
Rfx2	T	C	17: 56,803,722	Y88C	probably damaging	Het
Rpl6	T	C	5: 121,208,478	V214A	probably benign	Het
Rspo1	T	A	4: 124,991,432		probably null	Het
Sav1	A	C	12: 69,966,205	L366V	probably benign	Het
Sema3b	T	G	9: 107,604,156	T52P	possibly damaging	Het
Senp6	A	G	9: 80,116,559	D40G	probably damaging	Het
Sh3pxd2a	A	G	19: 47,268,207	S719P	probably damaging	Het
Shank1	C	T	7: 44,352,294	R1146W	unknown	Het
Slc34a3	G	T	2: 25,231,233	D307E	probably benign	Het
Slc35b3	T	C	13: 38,937,275	I330V	probably benign	Het
Slc4a10	G	A	2: 62,243,398	C268Y	probably benign	Het
Slco6d1	A	G	1: 98,421,399	E65G	probably benign	Het
Slit2	A	G	5: 48,245,573		probably benign	Het
Smcr8	T	C	11: 60,778,115	Y30H	probably damaging	Het
Snap47	A	G	11: 59,438,500		probably benign	Het
Snrnp25	G	A	11: 32,206,960	V15I	probably damaging	Het
Spns2	T	C	11: 72,454,397	Y449C	probably damaging	Het
Stab2	T	A	10: 86,924,450		probably benign	Het
Strip1	C	A	3: 107,614,613	D750Y	probably damaging	Het
Taf1c	A	T	8: 119,599,983	I438N	probably damaging	Het
Tbc1d2	T	A	4: 46,609,003	K745*	probably null	Het
Tctn1	T	C	5: 122,264,144	T76A	probably damaging	Het
Tfpi	T	C	2: 84,443,320		probably benign	Het
Timm44	A	T	8: 4,266,592	H317Q	probably benign	Het
Tnfaip1	A	T	11: 78,530,014		probably benign	Het
Tnpo3	A	T	6: 29,554,993		probably benign	Het
Top3b	T	C	16: 16,879,437		probably benign	Het
Trak1	T	A	9: 121,453,285	M410K	possibly damaging	Het
Trpm3	C	A	19: 22,978,654	P1160Q	probably damaging	Het
Tyk2	T	A	9: 21,111,137	T799S	possibly damaging	Het
Ubfd1	G	A	7: 122,067,491		probably benign	Het
Unc13a	G	A	8: 71,642,173	R1272*	probably null	Het
Unc45b	T	A	11: 82,940,205	L797Q	possibly damaging	Het
Urb2	C	T	8: 124,030,970	Q1139*	probably null	Het
Vmn2r66	A	T	7: 85,007,862	S112T	probably benign	Het
Vmn2r71	A	T	7: 85,623,634	D552V	probably benign	Het
Vmn2r76	A	G	7: 86,228,696	Y498H	probably benign	Het
Vmn2r84	A	C	10: 130,391,115	W285G	probably damaging	Het
Vps72	G	T	3: 95,122,583	L304F	probably damaging	Het
Wiz	A	T	17: 32,356,441	I907N	probably damaging	Het
Yaf2	T	C	15: 93,285,536	K131R	probably damaging	Het
Zfp141	A	T	7: 42,476,056	Y331N	possibly damaging	Het
Zfp324	G	T	7: 12,971,024	C380F	probably damaging	Het
Zfp521	T	C	18: 13,845,062	T765A	probably benign	Het
Zfp616	A	T	11: 74,085,674	H923L	probably damaging	Het
Zfpm1	C	T	8: 122,335,846	T548M	probably damaging	Het
Zp2	A	T	7: 120,143,576		probably benign	Het

Other mutations in Nalcn

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00952:Nalcn	APN	14	123348789	missense	probably benign	0.00
IGL00964:Nalcn	APN	14	123295384	splice site	probably benign
IGL01310:Nalcn	APN	14	123317249	missense	probably benign	0.00
IGL01578:Nalcn	APN	14	123572091	missense	probably benign	0.00
IGL01925:Nalcn	APN	14	123291848	missense	possibly damaging	0.88
IGL02072:Nalcn	APN	14	123323358	missense	probably benign	0.05
IGL02096:Nalcn	APN	14	123594503	missense	probably benign	0.11
IGL02212:Nalcn	APN	14	123515330	missense	probably damaging	0.99
IGL02306:Nalcn	APN	14	123323338	missense	probably benign	0.07
IGL02471:Nalcn	APN	14	123323314	missense	probably benign	0.02
IGL02478:Nalcn	APN	14	123321305	missense	probably benign	0.26
IGL02551:Nalcn	APN	14	123323338	missense	probably benign	0.07
IGL02630:Nalcn	APN	14	123317879	missense	probably benign	0.16
IGL02632:Nalcn	APN	14	123317853	missense	probably benign	0.11
IGL02661:Nalcn	APN	14	123592909	splice site	probably benign
IGL02830:Nalcn	APN	14	123293469	missense	probably damaging	0.98
IGL02939:Nalcn	APN	14	123298872	missense	probably null	1.00
IGL03035:Nalcn	APN	14	123278218	nonsense	probably null
IGL03226:Nalcn	APN	14	123281115	missense	probably benign	0.00
IGL03242:Nalcn	APN	14	123321487	missense	possibly damaging	0.91
Narnia	UTSW	14	123291047	missense	probably benign	0.11
R0019:Nalcn	UTSW	14	123507489	missense	probably benign	0.18
R0144:Nalcn	UTSW	14	123371536	missense	probably damaging	0.96
R0144:Nalcn	UTSW	14	123409839	splice site	probably benign
R0359:Nalcn	UTSW	14	123299168	missense	probably damaging	1.00
R0383:Nalcn	UTSW	14	123507559	missense	probably benign	0.01
R0400:Nalcn	UTSW	14	123290960	splice site	probably benign
R0467:Nalcn	UTSW	14	123291047	missense	probably benign	0.11
R0506:Nalcn	UTSW	14	123596614	missense	possibly damaging	0.82
R0583:Nalcn	UTSW	14	123294343	missense	possibly damaging	0.46
R0620:Nalcn	UTSW	14	123299141	splice site	probably benign
R0624:Nalcn	UTSW	14	123370032	missense	probably benign
R1381:Nalcn	UTSW	14	123314105	missense	probably damaging	1.00
R1467:Nalcn	UTSW	14	123464656	splice site	probably benign
R1689:Nalcn	UTSW	14	123285254	missense	probably damaging	1.00
R1726:Nalcn	UTSW	14	123308404	missense	probably damaging	1.00
R1774:Nalcn	UTSW	14	123278266	missense	probably benign
R1854:Nalcn	UTSW	14	123460412	missense	probably damaging	1.00
R1869:Nalcn	UTSW	14	123594553	missense	possibly damaging	0.96
R1871:Nalcn	UTSW	14	123594553	missense	possibly damaging	0.96
R1873:Nalcn	UTSW	14	123283601	missense	probably benign	0.00
R1899:Nalcn	UTSW	14	123316126	missense	possibly damaging	0.50
R1915:Nalcn	UTSW	14	123302769	missense	probably benign	0.08
R2016:Nalcn	UTSW	14	123594581	splice site	probably null
R2034:Nalcn	UTSW	14	123283603	missense	probably benign	0.01
R2087:Nalcn	UTSW	14	123281145	missense	probably benign
R2149:Nalcn	UTSW	14	123370017	missense	probably benign	0.01
R2157:Nalcn	UTSW	14	123409752	missense	probably benign	0.32
R2166:Nalcn	UTSW	14	123369951	missense	probably benign	0.00
R2932:Nalcn	UTSW	14	123593018	missense	probably benign	0.06
R3408:Nalcn	UTSW	14	123596617	missense	probably null	0.98
R3778:Nalcn	UTSW	14	123464716	missense	probably damaging	1.00
R3807:Nalcn	UTSW	14	123278187	missense	probably damaging	1.00
R3835:Nalcn	UTSW	14	123293422	splice site	probably benign
R3937:Nalcn	UTSW	14	123369945	missense	probably benign	0.00
R4001:Nalcn	UTSW	14	123596594	missense	probably damaging	1.00
R4015:Nalcn	UTSW	14	123486387	missense	probably damaging	1.00
R4033:Nalcn	UTSW	14	123599989	splice site	probably benign
R4231:Nalcn	UTSW	14	123599913	missense	probably benign	0.01
R4464:Nalcn	UTSW	14	123323350	missense	probably benign
R4512:Nalcn	UTSW	14	123295448	missense	probably damaging	1.00
R4542:Nalcn	UTSW	14	123321477	synonymous	silent
R4557:Nalcn	UTSW	14	123321235	intron	probably benign
R4869:Nalcn	UTSW	14	123599884	missense	probably benign	0.44
R5083:Nalcn	UTSW	14	123323294	splice site	probably null
R5109:Nalcn	UTSW	14	123278238	missense	possibly damaging	0.86
R5131:Nalcn	UTSW	14	123515770	missense	probably damaging	0.98
R5158:Nalcn	UTSW	14	123515737	missense	probably damaging	1.00
R5259:Nalcn	UTSW	14	123515651	missense	possibly damaging	0.94
R5422:Nalcn	UTSW	14	123515365	missense	probably damaging	1.00
R5514:Nalcn	UTSW	14	123283711	missense	probably benign	0.14
R5523:Nalcn	UTSW	14	123409743	missense	probably damaging	1.00
R5551:Nalcn	UTSW	14	123278286	missense	possibly damaging	0.57
R5667:Nalcn	UTSW	14	123295406	missense	probably damaging	1.00
R5671:Nalcn	UTSW	14	123295406	missense	probably damaging	1.00
R5750:Nalcn	UTSW	14	123572038	missense	probably benign
R5765:Nalcn	UTSW	14	123464726	missense	possibly damaging	0.46
R6324:Nalcn	UTSW	14	123409749	missense	possibly damaging	0.83
R6523:Nalcn	UTSW	14	123317843	missense	probably benign	0.00
R6558:Nalcn	UTSW	14	123486507	missense	probably benign
R6631:Nalcn	UTSW	14	123460251	missense	probably benign	0.17
R6667:Nalcn	UTSW	14	123321323	missense	probably damaging	1.00
R6670:Nalcn	UTSW	14	123464672	missense	possibly damaging	0.96
R6724:Nalcn	UTSW	14	123298067	missense	probably damaging	0.99
R6731:Nalcn	UTSW	14	123599934	missense	probably benign	0.22
R6957:Nalcn	UTSW	14	123507554	missense	probably damaging	0.96
R6970:Nalcn	UTSW	14	123314094	missense	possibly damaging	0.46
R7010:Nalcn	UTSW	14	123293465	missense	probably damaging	1.00
R7018:Nalcn	UTSW	14	123409821	missense	probably damaging	1.00
R7040:Nalcn	UTSW	14	123287855	missense	probably benign
R7089:Nalcn	UTSW	14	123278349	missense	probably benign	0.01
R7128:Nalcn	UTSW	14	123594502	missense	probably damaging	0.99
R7149:Nalcn	UTSW	14	123599865	missense	probably benign	0.02
R7361:Nalcn	UTSW	14	123291839	missense	probably benign	0.00
R7378:Nalcn	UTSW	14	123302890	missense	probably damaging	1.00
R7408:Nalcn	UTSW	14	123291860	missense	probably benign	0.00
R7470:Nalcn	UTSW	14	123572044	missense	probably benign	0.09
R7483:Nalcn	UTSW	14	123314087	missense	probably damaging	1.00
R7521:Nalcn	UTSW	14	123293458	missense	probably damaging	1.00
R7558:Nalcn	UTSW	14	123486385	critical splice donor site	probably null
R7585:Nalcn	UTSW	14	123515638	missense	probably damaging	1.00
R7591:Nalcn	UTSW	14	123323885	missense	probably benign	0.01
R7761:Nalcn	UTSW	14	123294379	missense	probably damaging	1.00
R7761:Nalcn	UTSW	14	123294380	missense	probably damaging	1.00
R7811:Nalcn	UTSW	14	123298945	missense	probably damaging	1.00
R7983:Nalcn	UTSW	14	123592997	missense	probably benign	0.17
R8089:Nalcn	UTSW	14	123299960	missense	probably damaging	1.00
R8110:Nalcn	UTSW	14	123464701	missense	probably benign	0.00
R8190:Nalcn	UTSW	14	123599939	missense	possibly damaging	0.69
R8273:Nalcn	UTSW	14	123317024	missense	probably damaging	1.00
R8407:Nalcn	UTSW	14	123317271	missense	probably damaging	1.00
R8497:Nalcn	UTSW	14	123515359	missense	probably damaging	1.00
R8544:Nalcn	UTSW	14	123371523	missense	probably benign	0.40
R8549:Nalcn	UTSW	14	123370036	missense	probably benign	0.01
R8731:Nalcn	UTSW	14	123599854	missense	probably benign	0.01
R8862:Nalcn	UTSW	14	123409787	missense	possibly damaging	0.96
R8919:Nalcn	UTSW	14	123323872	missense	probably benign	0.00
R9072:Nalcn	UTSW	14	123295451	missense	possibly damaging	0.66
R9073:Nalcn	UTSW	14	123295451	missense	possibly damaging	0.66
R9182:Nalcn	UTSW	14	123596604	missense	probably damaging	1.00
R9193:Nalcn	UTSW	14	123308380	nonsense	probably null
R9241:Nalcn	UTSW	14	123572017	missense	probably benign	0.00
R9267:Nalcn	UTSW	14	123281155	missense	probably benign	0.08
R9274:Nalcn	UTSW	14	123515656	missense	probably damaging	1.00
R9277:Nalcn	UTSW	14	123281111	missense	probably damaging	0.98
R9376:Nalcn	UTSW	14	123278301	missense	possibly damaging	0.74
X0060:Nalcn	UTSW	14	123285241	missense	probably damaging	1.00
Z1177:Nalcn	UTSW	14	123294445	missense	probably damaging	1.00
Z1177:Nalcn	UTSW	14	123594568	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGAATCTCCCTCAGAGCCATATTACCAA -3'
(R):5'- TGAAGCATCATCCTCAGAGCAGGT -3'

Sequencing Primer
(F):5'- GCAACTTCCCTTTTCCTGAGGA -3'
(R):5'- gcagacacccacactcac -3'

Posted On

2013-11-07