Incidental Mutation 'R0884:Mup3'
ID81020
Institutional Source Beutler Lab
Gene Symbol Mup3
Ensembl Gene ENSMUSG00000066154
Gene Namemajor urinary protein 3
SynonymsMup-3
MMRRC Submission 039051-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.055) question?
Stock #R0884 (G1)
Quality Score225
Status Not validated
Chromosome4
Chromosomal Location62083476-62087342 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to A at 62087174 bp
ZygosityHeterozygous
Amino Acid Change Isoleucine to Phenylalanine at position 20 (I20F)
Ref Sequence ENSEMBL: ENSMUSP00000103112 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000084531] [ENSMUST00000107472] [ENSMUST00000107488]
Predicted Effect possibly damaging
Transcript: ENSMUST00000084531
AA Change: I20F

PolyPhen 2 Score 0.676 (Sensitivity: 0.86; Specificity: 0.92)
SMART Domains Protein: ENSMUSP00000081579
Gene: ENSMUSG00000066154
AA Change: I20F

DomainStartEndE-ValueType
signal peptide 1 22 N/A INTRINSIC
Pfam:Lipocalin 38 177 2.2e-37 PFAM
Predicted Effect possibly damaging
Transcript: ENSMUST00000107472
AA Change: I20F

PolyPhen 2 Score 0.676 (Sensitivity: 0.86; Specificity: 0.92)
SMART Domains Protein: ENSMUSP00000103096
Gene: ENSMUSG00000066154
AA Change: I20F

DomainStartEndE-ValueType
signal peptide 1 22 N/A INTRINSIC
Pfam:Lipocalin 38 177 2.2e-37 PFAM
Predicted Effect possibly damaging
Transcript: ENSMUST00000107488
AA Change: I20F

PolyPhen 2 Score 0.676 (Sensitivity: 0.86; Specificity: 0.92)
SMART Domains Protein: ENSMUSP00000103112
Gene: ENSMUSG00000066154
AA Change: I20F

DomainStartEndE-ValueType
signal peptide 1 22 N/A INTRINSIC
Pfam:Lipocalin 38 177 2.2e-37 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000134190
Coding Region Coverage
  • 1x: 99.4%
  • 3x: 98.8%
  • 10x: 97.4%
  • 20x: 94.7%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 54 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abcc4 A T 14: 118,553,288 I844N possibly damaging Het
Acbd3 CGAGGAGGAGGAGGAGGA CGAGGAGGAGGAGGA 1: 180,747,059 probably benign Het
Adam6b A T 12: 113,490,995 R477S probably damaging Het
Aqp7 T A 4: 41,034,929 T175S possibly damaging Het
Bclaf1 A T 10: 20,322,076 R22* probably null Het
Bend7 A T 2: 4,744,244 K57N probably damaging Het
Bicc1 A C 10: 70,958,847 V160G probably damaging Het
Cacna2d4 C T 6: 119,307,286 R745W probably damaging Het
Cep72 A G 13: 74,054,881 probably null Het
Cobl A G 11: 12,375,908 I196T possibly damaging Het
Cux1 T G 5: 136,307,835 D941A probably damaging Het
Cyp2a12 T C 7: 27,032,542 I236T probably benign Het
Depdc5 T A 5: 32,917,978 V500D possibly damaging Het
Dhx35 T C 2: 158,831,711 I354T probably damaging Het
Dnase1l2 A G 17: 24,441,880 V170A possibly damaging Het
Eif4g3 C A 4: 138,151,776 N588K possibly damaging Het
Epsti1 A T 14: 77,931,275 R117S probably damaging Het
Fam53a T C 5: 33,600,816 E321G probably benign Het
Fat4 C A 3: 38,982,858 T3553K possibly damaging Het
Fer1l4 T C 2: 156,019,313 T1978A possibly damaging Het
Gabarapl2 A T 8: 111,942,505 I32F probably damaging Het
Gje1 G T 10: 14,716,740 S99R possibly damaging Het
Gm13083 A G 4: 143,615,184 D61G probably benign Het
Gosr1 A G 11: 76,730,146 I239T probably benign Het
Gpnmb T C 6: 49,047,913 V293A possibly damaging Het
Hyi T A 4: 118,360,217 I62N probably damaging Het
Il4ra A G 7: 125,574,663 I267V probably damaging Het
Kcnd2 A T 6: 21,216,541 Q81H probably benign Het
Ksr1 A T 11: 79,021,503 H675Q possibly damaging Het
Lpcat4 A G 2: 112,242,732 N208S probably damaging Het
Muc3 T A 5: 137,142,298 T162S possibly damaging Het
Nebl A C 2: 17,411,118 S327A probably benign Het
Nfatc4 A C 14: 55,826,644 D126A probably damaging Het
Nmt2 A T 2: 3,314,785 R271* probably null Het
Nol7 G A 13: 43,400,615 V133I probably benign Het
Olfr376 A T 11: 73,374,889 I47F probably benign Het
Olfr610 C T 7: 103,506,862 W28* probably null Het
Pde4d A T 13: 109,950,940 I670L probably damaging Het
Phlpp1 T A 1: 106,389,665 probably null Het
Pigz A G 16: 31,941,976 probably null Het
Prrg4 A T 2: 104,839,362 Y137N probably damaging Het
Rbm28 A T 6: 29,155,154 S217T possibly damaging Het
Ryr2 T C 13: 11,554,529 D4963G probably damaging Het
Slc35b2 T A 17: 45,566,825 F293I probably damaging Het
Slc35f1 A T 10: 53,089,347 Y286F probably damaging Het
Slc6a18 G T 13: 73,667,037 A384D probably damaging Het
St5 A G 7: 109,557,345 L66P probably damaging Het
Syt9 A G 7: 107,436,561 I262V probably damaging Het
Tln2 C A 9: 67,370,733 R333L probably damaging Het
Tnrc6b ACAGCAGCAGCAGCAGCAGCAG ACAGCAGCAGCAGCAGCAG 15: 80,902,555 probably benign Het
Ttn G A 2: 76,751,066 T14834I possibly damaging Het
Uggt1 T C 1: 36,175,078 S177G probably benign Het
Zfp454 A G 11: 50,873,937 S223P probably benign Het
Zmat4 A G 8: 24,015,127 T128A probably benign Het
Other mutations in Mup3
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01383:Mup3 APN 4 62085959 missense probably damaging 0.98
IGL02233:Mup3 APN 4 62084779 missense probably damaging 1.00
IGL03088:Mup3 APN 4 62086842 missense probably damaging 0.99
R0432:Mup3 UTSW 4 62085282 missense probably benign 0.13
R0619:Mup3 UTSW 4 62085961 missense probably benign 0.02
R5568:Mup3 UTSW 4 62084572 missense possibly damaging 0.93
R5849:Mup3 UTSW 4 62086935 critical splice acceptor site probably null
R5909:Mup3 UTSW 4 62086007 missense probably benign 0.01
R7851:Mup3 UTSW 4 62085257 missense possibly damaging 0.65
R7934:Mup3 UTSW 4 62085257 missense possibly damaging 0.65
Z1088:Mup3 UTSW 4 62087189 missense unknown
Predicted Primers PCR Primer
(F):5'- CAAAAGCTCTCATGCTGCCATGTTC -3'
(R):5'- AAAGCCTGACTGAGGTAATGCAGAC -3'

Sequencing Primer
(F):5'- GTTTCTATTTGAACTCCGCACTATG -3'
(R):5'- CTGAGGTAATGCAGACCCATACG -3'
Posted On2013-11-07