Mutagenetix > Incidental Mutation

Incidental Mutation 'R0976:Vmn2r69'

81163

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r69

Ensembl Gene

ENSMUSG00000091006

Gene Name

vomeronasal 2, receptor 69

Synonyms

MMRRC Submission

039105-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.074) question?

Stock #

R0976 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

85055584-85064884 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 85056108 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 677 (T677A)

Ref Sequence

ENSEMBL: ENSMUSP00000132726 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000171213]

AlphaFold

G3XA45

Predicted Effect

probably damaging
Transcript: ENSMUST00000171213
AA Change: T677A

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000132726
Gene: ENSMUSG00000091006
AA Change: T677A

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	465	1.3e-28	PFAM
Pfam:NCD3G	507	559	1.8e-20	PFAM
Pfam:7tm_3	592	827	3.2e-54	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000207880

Coding Region Coverage

1x: 99.8%
3x: 99.3%
10x: 97.6%
20x: 95.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 37 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700056E22Rik	C	T	1: 183,765,702 (GRCm39)	S119N	probably benign	Het
Arap2	T	A	5: 62,807,227 (GRCm39)	I1147F	probably damaging	Het
Arl10	T	C	13: 54,723,621 (GRCm39)		probably benign	Het
Axin1	A	G	17: 26,407,060 (GRCm39)	E551G	probably damaging	Het
Ccr6	T	A	17: 8,475,254 (GRCm39)	L153Q	probably damaging	Het
Cntnap2	C	T	6: 47,248,164 (GRCm39)	P1190L	probably damaging	Het
Cul7	T	A	17: 46,974,116 (GRCm39)	L1467H	probably damaging	Het
Cux1	T	A	5: 136,342,144 (GRCm39)	D416V	probably damaging	Het
Cyp2c67	G	T	19: 39,631,818 (GRCm39)	F126L	probably damaging	Het
Cyp3a57	A	G	5: 145,327,278 (GRCm39)	I490V	probably benign	Het
Dsc1	T	C	18: 20,228,098 (GRCm39)		probably null	Het
Fam83f	T	A	15: 80,576,285 (GRCm39)	V312E	probably damaging	Het
Fsip2	A	G	2: 82,828,375 (GRCm39)	D6724G	possibly damaging	Het
Gabrr3	G	T	16: 59,281,887 (GRCm39)	C414F	probably benign	Het
Gm21738	T	C	14: 19,415,963 (GRCm38)	K192R	probably benign	Het
H2bc18	T	A	3: 96,177,402 (GRCm39)	V112E	probably benign	Het
Herc1	A	T	9: 66,347,160 (GRCm39)	K2005M	possibly damaging	Het
Isyna1	C	A	8: 71,048,936 (GRCm39)	N338K	probably damaging	Het
Kalrn	T	C	16: 34,205,760 (GRCm39)	D39G	probably damaging	Het
Mndal	T	A	1: 173,690,411 (GRCm39)	R306S	possibly damaging	Het
Nek2	A	G	1: 191,559,349 (GRCm39)	R285G	probably benign	Het
Nrg2	A	G	18: 36,154,144 (GRCm39)	I591T	probably benign	Het
Or8u8	A	G	2: 86,012,152 (GRCm39)	L101S	probably damaging	Het
Pcdh10	T	C	3: 45,335,236 (GRCm39)	S517P	probably damaging	Het
Pdcd2l	G	T	7: 33,895,771 (GRCm39)	D67E	probably benign	Het
Pex1	C	A	5: 3,683,943 (GRCm39)	D1146E	probably benign	Het
Pid1	A	T	1: 84,136,946 (GRCm39)	Y62N	probably benign	Het
Ppp4r1	T	C	17: 66,148,013 (GRCm39)	*935R	probably null	Het
Stag1	T	C	9: 100,658,877 (GRCm39)	F155L	probably damaging	Het
Stag1	G	A	9: 100,812,069 (GRCm39)		probably null	Het
Taok2	C	T	7: 126,474,323 (GRCm39)	R302Q	possibly damaging	Het
Tbc1d8	A	G	1: 39,445,882 (GRCm39)	V103A	probably damaging	Het
Terf2ip	T	A	8: 112,738,349 (GRCm39)	I79N	probably damaging	Het
Tgfb3	G	A	12: 86,116,606 (GRCm39)	T144I	probably damaging	Het
Top1	A	G	2: 160,559,343 (GRCm39)	N622S	possibly damaging	Het
Trappc9	T	A	15: 72,871,823 (GRCm39)	Q489L	probably damaging	Het
Wdr35	T	C	12: 9,036,104 (GRCm39)	F292L	probably benign	Het

Other mutations in Vmn2r69

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01019:Vmn2r69	APN	7	85,055,739 (GRCm39)	missense	probably benign
IGL01457:Vmn2r69	APN	7	85,055,836 (GRCm39)	missense	possibly damaging	0.87
IGL01760:Vmn2r69	APN	7	85,056,072 (GRCm39)	missense	possibly damaging	0.90
IGL01834:Vmn2r69	APN	7	85,061,576 (GRCm39)	missense	probably damaging	1.00
IGL02001:Vmn2r69	APN	7	85,056,434 (GRCm39)	missense	probably benign	0.05
IGL02057:Vmn2r69	APN	7	85,060,990 (GRCm39)	missense	possibly damaging	0.93
IGL02289:Vmn2r69	APN	7	85,056,054 (GRCm39)	missense	probably damaging	1.00
IGL02472:Vmn2r69	APN	7	85,058,960 (GRCm39)	missense	probably benign	0.01
IGL02478:Vmn2r69	APN	7	85,055,889 (GRCm39)	missense	probably damaging	1.00
IGL02554:Vmn2r69	APN	7	85,059,014 (GRCm39)	missense	probably damaging	1.00
IGL02723:Vmn2r69	APN	7	85,059,416 (GRCm39)	missense	probably damaging	1.00
R0526:Vmn2r69	UTSW	7	85,060,711 (GRCm39)	missense	probably damaging	1.00
R0560:Vmn2r69	UTSW	7	85,058,922 (GRCm39)	critical splice donor site	probably null
R0909:Vmn2r69	UTSW	7	85,055,873 (GRCm39)	missense	probably benign	0.00
R1158:Vmn2r69	UTSW	7	85,059,058 (GRCm39)	splice site	probably benign
R1459:Vmn2r69	UTSW	7	85,055,908 (GRCm39)	nonsense	probably null
R1482:Vmn2r69	UTSW	7	85,056,082 (GRCm39)	missense	probably damaging	1.00
R1917:Vmn2r69	UTSW	7	85,060,891 (GRCm39)	missense	probably damaging	1.00
R2016:Vmn2r69	UTSW	7	85,056,493 (GRCm39)	missense	probably damaging	0.98
R2108:Vmn2r69	UTSW	7	85,059,404 (GRCm39)	missense	probably benign
R2571:Vmn2r69	UTSW	7	85,064,764 (GRCm39)	missense	probably benign
R2910:Vmn2r69	UTSW	7	85,055,918 (GRCm39)	missense	probably damaging	1.00
R2920:Vmn2r69	UTSW	7	85,060,973 (GRCm39)	missense	probably benign	0.08
R3708:Vmn2r69	UTSW	7	85,061,029 (GRCm39)	missense	probably damaging	0.98
R3710:Vmn2r69	UTSW	7	85,055,601 (GRCm39)	missense	probably benign
R4757:Vmn2r69	UTSW	7	85,061,575 (GRCm39)	missense	probably damaging	0.99
R4823:Vmn2r69	UTSW	7	85,060,508 (GRCm39)	missense	probably benign	0.21
R4870:Vmn2r69	UTSW	7	85,060,793 (GRCm39)	missense	possibly damaging	0.93
R4918:Vmn2r69	UTSW	7	85,055,967 (GRCm39)	missense	probably benign	0.06
R5022:Vmn2r69	UTSW	7	85,060,367 (GRCm39)	missense	possibly damaging	0.72
R5174:Vmn2r69	UTSW	7	85,064,739 (GRCm39)	missense	possibly damaging	0.92
R5200:Vmn2r69	UTSW	7	85,055,717 (GRCm39)	missense	probably damaging	1.00
R5278:Vmn2r69	UTSW	7	85,060,991 (GRCm39)	missense	probably benign	0.02
R5643:Vmn2r69	UTSW	7	85,056,404 (GRCm39)	missense	probably damaging	0.98
R5996:Vmn2r69	UTSW	7	85,061,117 (GRCm39)	splice site	probably null
R6083:Vmn2r69	UTSW	7	85,055,711 (GRCm39)	missense	probably damaging	1.00
R6140:Vmn2r69	UTSW	7	85,060,657 (GRCm39)	missense	probably damaging	0.99
R6306:Vmn2r69	UTSW	7	85,064,799 (GRCm39)	missense	probably benign	0.04
R6330:Vmn2r69	UTSW	7	85,060,835 (GRCm39)	missense	probably benign
R6380:Vmn2r69	UTSW	7	85,061,067 (GRCm39)	missense	probably benign
R6466:Vmn2r69	UTSW	7	85,056,378 (GRCm39)	missense	probably benign	0.01
R6542:Vmn2r69	UTSW	7	85,060,413 (GRCm39)	nonsense	probably null
R6583:Vmn2r69	UTSW	7	85,059,017 (GRCm39)	missense	probably benign
R6623:Vmn2r69	UTSW	7	85,056,309 (GRCm39)	missense	possibly damaging	0.84
R6709:Vmn2r69	UTSW	7	85,061,069 (GRCm39)	missense	probably benign	0.03
R6732:Vmn2r69	UTSW	7	85,060,351 (GRCm39)	missense	probably benign	0.00
R6741:Vmn2r69	UTSW	7	85,061,724 (GRCm39)	missense	probably benign	0.01
R7070:Vmn2r69	UTSW	7	85,060,688 (GRCm39)	missense	probably damaging	0.98
R7234:Vmn2r69	UTSW	7	85,056,315 (GRCm39)	missense	probably benign	0.22
R7323:Vmn2r69	UTSW	7	85,060,972 (GRCm39)	missense	possibly damaging	0.95
R7427:Vmn2r69	UTSW	7	85,060,467 (GRCm39)	missense	probably benign	0.28
R7428:Vmn2r69	UTSW	7	85,060,467 (GRCm39)	missense	probably benign	0.28
R7453:Vmn2r69	UTSW	7	85,060,768 (GRCm39)	frame shift	probably null
R7532:Vmn2r69	UTSW	7	85,059,622 (GRCm39)	missense	probably benign	0.36
R7556:Vmn2r69	UTSW	7	85,060,768 (GRCm39)	frame shift	probably null
R7562:Vmn2r69	UTSW	7	85,056,420 (GRCm39)	missense	probably benign
R7592:Vmn2r69	UTSW	7	85,060,768 (GRCm39)	frame shift	probably null
R7708:Vmn2r69	UTSW	7	85,061,755 (GRCm39)	missense	possibly damaging	0.87
R7803:Vmn2r69	UTSW	7	85,056,324 (GRCm39)	missense	probably benign	0.00
R7960:Vmn2r69	UTSW	7	85,055,973 (GRCm39)	missense	probably benign
R7966:Vmn2r69	UTSW	7	85,060,762 (GRCm39)	missense	possibly damaging	0.81
R8071:Vmn2r69	UTSW	7	85,055,713 (GRCm39)	nonsense	probably null
R8237:Vmn2r69	UTSW	7	85,060,340 (GRCm39)	missense	probably benign	0.02
R8347:Vmn2r69	UTSW	7	85,064,838 (GRCm39)	missense	probably benign	0.00
R8737:Vmn2r69	UTSW	7	85,055,783 (GRCm39)	missense	probably damaging	1.00
R8795:Vmn2r69	UTSW	7	85,064,883 (GRCm39)	start codon destroyed	probably null	0.94
R8831:Vmn2r69	UTSW	7	85,059,018 (GRCm39)	nonsense	probably null
R8856:Vmn2r69	UTSW	7	85,061,663 (GRCm39)	missense	probably benign	0.00
R8998:Vmn2r69	UTSW	7	85,060,307 (GRCm39)	missense	probably benign	0.33
R8999:Vmn2r69	UTSW	7	85,060,307 (GRCm39)	missense	probably benign	0.33
R9161:Vmn2r69	UTSW	7	85,056,177 (GRCm39)	missense	possibly damaging	0.88
R9228:Vmn2r69	UTSW	7	85,064,697 (GRCm39)	missense	probably benign	0.01
R9494:Vmn2r69	UTSW	7	85,060,768 (GRCm39)	missense	probably damaging	1.00
R9494:Vmn2r69	UTSW	7	85,056,084 (GRCm39)	missense	probably benign	0.08
R9541:Vmn2r69	UTSW	7	85,056,209 (GRCm39)	missense	probably benign
R9620:Vmn2r69	UTSW	7	85,061,504 (GRCm39)	missense	probably benign	0.10
Z1176:Vmn2r69	UTSW	7	85,055,696 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGCAACCATAGTCTTGCCTTTGGTG -3'
(R):5'- GAGACCCTTTGGGAAAAGCTCTGG -3'

Sequencing Primer
(F):5'- AATGTCAAGAGCTTGGCCTC -3'
(R):5'- TGTCTTCCTGAAACACAGAGATACTC -3'

Posted On

2013-11-07