|Institutional Source||Beutler Lab|
|Gene Name||lin-28 homolog B (C. elegans)|
|Is this an essential gene?||Not available|
|Stock #||R0885 (G1)|
|Chromosomal Location||45376620-45486395 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||C to T at 45381228 bp|
|Amino Acid Change||Glycine to Glutamic Acid at position 218 (G218E)|
|Ref Sequence||ENSEMBL: ENSMUSP00000078361 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000079390]|
|Predicted Effect||probably damaging
AA Change: G218E
PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)
AA Change: G218E
|Coding Region Coverage||
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene belongs to the lin-28 family, which is characterized by the presence of a cold-shock domain and a pair of CCHC zinc finger domains. This gene is highly expressed in testis, fetal liver, placenta, and in primary human tumors and cancer cell lines. It is negatively regulated by microRNAs that target sites in the 3' UTR, and overexpression of this gene in primary tumors is linked to the repression of let-7 family of microRNAs and derepression of let-7 targets, which facilitates cellular transformation. [provided by RefSeq, Jun 2012]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit postnatal dwarfism in male, but not female, mice. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Lin28b||
(F):5'- CTGTGCTTGCATGAGGTAGACTTCC -3'
(R):5'- TGCTACAACTGTGGTGGTCTCGAC -3'
(F):5'- TGAGGTAGACTTCCCGCCC -3'
(R):5'- TGGTCTCGACCATCATGCTAAAG -3'