Mutagenetix > Incidental Mutation

Incidental Mutation 'R0976:Cyp2c67'

81211

Institutional Source

Beutler Lab

Gene Symbol

Cyp2c67

Ensembl Gene

ENSMUSG00000062624

Gene Name

cytochrome P450, family 2, subfamily c, polypeptide 67

Synonyms

C730004C24Rik

MMRRC Submission

039105-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.077) question?

Stock #

R0976 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

39597288-39637497 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 39631818 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 126 (F126L)

Ref Sequence

ENSEMBL: ENSMUSP00000065796 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000067328]

AlphaFold

Q569X9

Predicted Effect

probably damaging
Transcript: ENSMUST00000067328
AA Change: F126L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000065796
Gene: ENSMUSG00000062624
AA Change: F126L

Domain	Start	End	E-Value	Type
signal peptide	1	25	N/A	INTRINSIC
Pfam:p450	30	487	8.5e-150	PFAM

Coding Region Coverage

1x: 99.8%
3x: 99.3%
10x: 97.6%
20x: 95.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 37 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700056E22Rik	C	T	1: 183,765,702 (GRCm39)	S119N	probably benign	Het
Arap2	T	A	5: 62,807,227 (GRCm39)	I1147F	probably damaging	Het
Arl10	T	C	13: 54,723,621 (GRCm39)		probably benign	Het
Axin1	A	G	17: 26,407,060 (GRCm39)	E551G	probably damaging	Het
Ccr6	T	A	17: 8,475,254 (GRCm39)	L153Q	probably damaging	Het
Cntnap2	C	T	6: 47,248,164 (GRCm39)	P1190L	probably damaging	Het
Cul7	T	A	17: 46,974,116 (GRCm39)	L1467H	probably damaging	Het
Cux1	T	A	5: 136,342,144 (GRCm39)	D416V	probably damaging	Het
Cyp3a57	A	G	5: 145,327,278 (GRCm39)	I490V	probably benign	Het
Dsc1	T	C	18: 20,228,098 (GRCm39)		probably null	Het
Fam83f	T	A	15: 80,576,285 (GRCm39)	V312E	probably damaging	Het
Fsip2	A	G	2: 82,828,375 (GRCm39)	D6724G	possibly damaging	Het
Gabrr3	G	T	16: 59,281,887 (GRCm39)	C414F	probably benign	Het
Gm21738	T	C	14: 19,415,963 (GRCm38)	K192R	probably benign	Het
H2bc18	T	A	3: 96,177,402 (GRCm39)	V112E	probably benign	Het
Herc1	A	T	9: 66,347,160 (GRCm39)	K2005M	possibly damaging	Het
Isyna1	C	A	8: 71,048,936 (GRCm39)	N338K	probably damaging	Het
Kalrn	T	C	16: 34,205,760 (GRCm39)	D39G	probably damaging	Het
Mndal	T	A	1: 173,690,411 (GRCm39)	R306S	possibly damaging	Het
Nek2	A	G	1: 191,559,349 (GRCm39)	R285G	probably benign	Het
Nrg2	A	G	18: 36,154,144 (GRCm39)	I591T	probably benign	Het
Or8u8	A	G	2: 86,012,152 (GRCm39)	L101S	probably damaging	Het
Pcdh10	T	C	3: 45,335,236 (GRCm39)	S517P	probably damaging	Het
Pdcd2l	G	T	7: 33,895,771 (GRCm39)	D67E	probably benign	Het
Pex1	C	A	5: 3,683,943 (GRCm39)	D1146E	probably benign	Het
Pid1	A	T	1: 84,136,946 (GRCm39)	Y62N	probably benign	Het
Ppp4r1	T	C	17: 66,148,013 (GRCm39)	*935R	probably null	Het
Stag1	T	C	9: 100,658,877 (GRCm39)	F155L	probably damaging	Het
Stag1	G	A	9: 100,812,069 (GRCm39)		probably null	Het
Taok2	C	T	7: 126,474,323 (GRCm39)	R302Q	possibly damaging	Het
Tbc1d8	A	G	1: 39,445,882 (GRCm39)	V103A	probably damaging	Het
Terf2ip	T	A	8: 112,738,349 (GRCm39)	I79N	probably damaging	Het
Tgfb3	G	A	12: 86,116,606 (GRCm39)	T144I	probably damaging	Het
Top1	A	G	2: 160,559,343 (GRCm39)	N622S	possibly damaging	Het
Trappc9	T	A	15: 72,871,823 (GRCm39)	Q489L	probably damaging	Het
Vmn2r69	T	C	7: 85,056,108 (GRCm39)	T677A	probably damaging	Het
Wdr35	T	C	12: 9,036,104 (GRCm39)	F292L	probably benign	Het

Other mutations in Cyp2c67

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00955:Cyp2c67	APN	19	39,631,829 (GRCm39)	missense	possibly damaging	0.95
IGL01025:Cyp2c67	APN	19	39,628,376 (GRCm39)	nonsense	probably null
IGL01363:Cyp2c67	APN	19	39,628,411 (GRCm39)	missense	probably damaging	0.99
IGL01819:Cyp2c67	APN	19	39,604,165 (GRCm39)	missense	probably damaging	0.98
IGL01902:Cyp2c67	APN	19	39,637,470 (GRCm39)	missense	probably damaging	1.00
IGL02172:Cyp2c67	APN	19	39,637,446 (GRCm39)	missense	possibly damaging	0.76
IGL02351:Cyp2c67	APN	19	39,605,861 (GRCm39)	missense	probably damaging	1.00
IGL02355:Cyp2c67	APN	19	39,605,826 (GRCm39)	nonsense	probably null
IGL02355:Cyp2c67	APN	19	39,631,849 (GRCm39)	missense	probably benign	0.34
IGL02358:Cyp2c67	APN	19	39,605,861 (GRCm39)	missense	probably damaging	1.00
IGL02362:Cyp2c67	APN	19	39,605,826 (GRCm39)	nonsense	probably null
IGL02362:Cyp2c67	APN	19	39,631,849 (GRCm39)	missense	probably benign	0.34
IGL02388:Cyp2c67	APN	19	39,631,799 (GRCm39)	missense	probably benign	0.20
IGL03106:Cyp2c67	APN	19	39,632,119 (GRCm39)	missense	probably benign	0.27
IGL03219:Cyp2c67	APN	19	39,631,738 (GRCm39)	missense	possibly damaging	0.54
IGL03326:Cyp2c67	APN	19	39,631,713 (GRCm39)	critical splice donor site	probably null
IGL03349:Cyp2c67	APN	19	39,632,128 (GRCm39)	missense	probably damaging	1.00
IGL03356:Cyp2c67	APN	19	39,628,405 (GRCm39)	missense	probably damaging	1.00
IGL03052:Cyp2c67	UTSW	19	39,637,329 (GRCm39)	missense	possibly damaging	0.88
R0585:Cyp2c67	UTSW	19	39,627,138 (GRCm39)	missense	possibly damaging	0.59
R0975:Cyp2c67	UTSW	19	39,597,622 (GRCm39)	missense	possibly damaging	0.49
R1252:Cyp2c67	UTSW	19	39,614,585 (GRCm39)	missense	possibly damaging	0.93
R1398:Cyp2c67	UTSW	19	39,627,069 (GRCm39)	missense	probably damaging	0.96
R1411:Cyp2c67	UTSW	19	39,627,035 (GRCm39)	missense	probably damaging	1.00
R1505:Cyp2c67	UTSW	19	39,637,408 (GRCm39)	missense	probably benign	0.00
R1543:Cyp2c67	UTSW	19	39,631,708 (GRCm39)	splice site	probably benign
R1613:Cyp2c67	UTSW	19	39,614,643 (GRCm39)	missense	probably benign	0.00
R1618:Cyp2c67	UTSW	19	39,631,708 (GRCm39)	splice site	probably benign
R1667:Cyp2c67	UTSW	19	39,632,034 (GRCm39)	critical splice donor site	probably null
R1852:Cyp2c67	UTSW	19	39,605,811 (GRCm39)	missense	probably benign	0.01
R2005:Cyp2c67	UTSW	19	39,631,789 (GRCm39)	missense	probably damaging	1.00
R2105:Cyp2c67	UTSW	19	39,614,681 (GRCm39)	missense	probably benign	0.24
R2181:Cyp2c67	UTSW	19	39,597,541 (GRCm39)	missense	possibly damaging	0.94
R3817:Cyp2c67	UTSW	19	39,627,127 (GRCm39)	missense	probably benign	0.00
R4669:Cyp2c67	UTSW	19	39,632,098 (GRCm39)	missense	probably benign	0.00
R4689:Cyp2c67	UTSW	19	39,627,032 (GRCm39)	missense	probably benign	0.00
R4756:Cyp2c67	UTSW	19	39,632,188 (GRCm39)	missense	probably benign	0.03
R4823:Cyp2c67	UTSW	19	39,604,168 (GRCm39)	missense	probably benign	0.13
R5152:Cyp2c67	UTSW	19	39,627,132 (GRCm39)	missense	probably benign	0.00
R5345:Cyp2c67	UTSW	19	39,614,676 (GRCm39)	missense	probably benign	0.01
R5580:Cyp2c67	UTSW	19	39,604,094 (GRCm39)	missense	probably damaging	0.99
R5644:Cyp2c67	UTSW	19	39,604,138 (GRCm39)	missense	possibly damaging	0.84
R6116:Cyp2c67	UTSW	19	39,605,879 (GRCm39)	missense	probably damaging	1.00
R6516:Cyp2c67	UTSW	19	39,605,873 (GRCm39)	missense	probably damaging	1.00
R6550:Cyp2c67	UTSW	19	39,605,854 (GRCm39)	nonsense	probably null
R6939:Cyp2c67	UTSW	19	39,631,778 (GRCm39)	missense	possibly damaging	0.68
R6995:Cyp2c67	UTSW	19	39,604,123 (GRCm39)	missense	probably damaging	0.96
R7028:Cyp2c67	UTSW	19	39,628,341 (GRCm39)	missense	possibly damaging	0.68
R7144:Cyp2c67	UTSW	19	39,604,138 (GRCm39)	missense	probably benign	0.00
R7242:Cyp2c67	UTSW	19	39,605,783 (GRCm39)	missense	probably benign	0.30
R7335:Cyp2c67	UTSW	19	39,628,451 (GRCm39)	nonsense	probably null
R7337:Cyp2c67	UTSW	19	39,597,708 (GRCm39)	splice site	probably null
R7474:Cyp2c67	UTSW	19	39,605,876 (GRCm39)	missense	probably null	0.05
R7642:Cyp2c67	UTSW	19	39,604,084 (GRCm39)	missense	probably damaging	0.97
R7870:Cyp2c67	UTSW	19	39,597,669 (GRCm39)	missense	probably damaging	1.00
R8152:Cyp2c67	UTSW	19	39,628,452 (GRCm39)	missense	probably benign	0.21
R8367:Cyp2c67	UTSW	19	39,627,118 (GRCm39)	missense	probably benign	0.01
R8717:Cyp2c67	UTSW	19	39,627,155 (GRCm39)	missense	probably benign	0.05
R8728:Cyp2c67	UTSW	19	39,614,605 (GRCm39)	missense	probably damaging	1.00
R9275:Cyp2c67	UTSW	19	39,597,699 (GRCm39)	missense	probably damaging	1.00
R9278:Cyp2c67	UTSW	19	39,597,699 (GRCm39)	missense	probably damaging	1.00
R9376:Cyp2c67	UTSW	19	39,627,178 (GRCm39)	missense	probably damaging	1.00
Z1177:Cyp2c67	UTSW	19	39,632,123 (GRCm39)	missense	possibly damaging	0.89

Predicted Primers

PCR Primer
(F):5'- ACCAGTGCTACATGGTCTATGCTCC -3'
(R):5'- AGTTTCCAGAGCAAGCTGAAAGGTG -3'

Sequencing Primer
(F):5'- tgctccttacttttacatggtaac -3'
(R):5'- GGAGAGTAGAGGTCATGCTTGC -3'

Posted On

2013-11-07