Mutagenetix > Incidental Mutation

Incidental Mutation 'R0939:Vmn2r71'

81307

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r71

Ensembl Gene

ENSMUSG00000091205

Gene Name

vomeronasal 2, receptor 71

Synonyms

EG233445

MMRRC Submission

039078-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.067) question?

Stock #

R0939 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

85264670-85273755 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 85272889 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 568 (T568S)

Ref Sequence

ENSEMBL: ENSMUSP00000132337 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000172338]

AlphaFold

L7N2D8

Predicted Effect

possibly damaging
Transcript: ENSMUST00000172338
AA Change: T568S

PolyPhen 2 Score 0.696 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000132337
Gene: ENSMUSG00000091205
AA Change: T568S

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	468	2.1e-31	PFAM
Pfam:NCD3G	511	563	8.7e-20	PFAM
Pfam:7tm_3	593	831	2e-55	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000208273

Coding Region Coverage

1x: 99.4%
3x: 98.7%
10x: 97.3%
20x: 94.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 30 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aatk	C	T	11: 119,902,969 (GRCm39)	V419M	probably damaging	Het
Cdcp1	A	G	9: 123,012,755 (GRCm39)	V264A	probably damaging	Het
Cfap53	A	G	18: 74,438,801 (GRCm39)	D326G	probably null	Het
Dach1	A	G	14: 98,153,360 (GRCm39)	V436A	probably damaging	Het
Dnah11	C	T	12: 118,024,142 (GRCm39)	G1870S	probably damaging	Het
Dst	C	A	1: 34,283,464 (GRCm39)	H5324N	probably damaging	Het
Eapp	TTTCTTCTTCTTCTTCTT	TTTCTTCTTCTTCTT	12: 54,732,734 (GRCm39)		probably benign	Het
Esd	C	A	14: 74,973,467 (GRCm39)	H21N	probably damaging	Het
Gnmt	A	G	17: 47,037,271 (GRCm39)	L171P	probably damaging	Het
Igdcc4	T	A	9: 65,038,755 (GRCm39)		probably null	Het
Mug1	A	T	6: 121,861,308 (GRCm39)	I1310F	possibly damaging	Het
Mybpc2	T	C	7: 44,156,311 (GRCm39)	K834R	probably benign	Het
Or10a3b	T	A	7: 108,444,440 (GRCm39)	Y259F	probably damaging	Het
Or10h5	T	C	17: 33,434,635 (GRCm39)	K231E	possibly damaging	Het
Or9g19	T	A	2: 85,600,997 (GRCm39)	L284*	probably null	Het
Pcdh17	A	G	14: 84,685,195 (GRCm39)	D554G	probably damaging	Het
Plcxd3	T	A	15: 4,546,344 (GRCm39)	L116*	probably null	Het
Prss1	A	C	6: 41,440,522 (GRCm39)	D199A	probably damaging	Het
Rbms3	C	T	9: 116,939,028 (GRCm39)		probably null	Het
Rif1	GCCACCA	GCCA	2: 52,000,336 (GRCm39)		probably benign	Het
Rreb1	A	G	13: 38,116,207 (GRCm39)	M1189V	probably benign	Het
Slc25a39	T	C	11: 102,295,877 (GRCm39)	E118G	probably damaging	Het
Slfn5	G	T	11: 82,852,164 (GRCm39)	M763I	probably benign	Het
Speer4f2	A	G	5: 17,579,402 (GRCm39)	E67G	probably damaging	Het
Spef2	A	T	15: 9,704,636 (GRCm39)		probably null	Het
Spocd1	A	T	4: 129,842,663 (GRCm39)	D26V	possibly damaging	Het
Ssh1	A	G	5: 114,108,497 (GRCm39)	L50P	probably damaging	Het
Tle1	A	G	4: 72,036,771 (GRCm39)	L728P	probably damaging	Het
Trio	A	G	15: 27,741,336 (GRCm39)		probably null	Het
Tubb1	G	A	2: 174,297,549 (GRCm39)	E53K	probably damaging	Het

Other mutations in Vmn2r71

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00922:Vmn2r71	APN	7	85,267,901 (GRCm39)	missense	probably benign
IGL00960:Vmn2r71	APN	7	85,273,582 (GRCm39)	missense	probably damaging	1.00
IGL01372:Vmn2r71	APN	7	85,270,022 (GRCm39)	splice site	probably benign
IGL01690:Vmn2r71	APN	7	85,264,782 (GRCm39)	missense	probably damaging	1.00
IGL01909:Vmn2r71	APN	7	85,270,001 (GRCm39)	missense	probably benign	0.00
IGL01950:Vmn2r71	APN	7	85,264,827 (GRCm39)	missense	probably damaging	0.98
IGL02570:Vmn2r71	APN	7	85,264,748 (GRCm39)	missense	possibly damaging	0.95
IGL02650:Vmn2r71	APN	7	85,273,535 (GRCm39)	missense	probably damaging	1.00
IGL02901:Vmn2r71	APN	7	85,268,470 (GRCm39)	missense	probably benign	0.00
IGL03128:Vmn2r71	APN	7	85,268,795 (GRCm39)	missense	probably damaging	1.00
IGL03328:Vmn2r71	APN	7	85,273,499 (GRCm39)	missense	probably damaging	1.00
R0533:Vmn2r71	UTSW	7	85,268,426 (GRCm39)	frame shift	probably null
R0707:Vmn2r71	UTSW	7	85,268,640 (GRCm39)	missense	probably benign
R0841:Vmn2r71	UTSW	7	85,267,749 (GRCm39)	missense	possibly damaging	0.62
R0865:Vmn2r71	UTSW	7	85,268,516 (GRCm39)	missense	probably benign	0.01
R0883:Vmn2r71	UTSW	7	85,272,842 (GRCm39)	missense	probably benign	0.19
R1597:Vmn2r71	UTSW	7	85,273,352 (GRCm39)	missense	possibly damaging	0.46
R1646:Vmn2r71	UTSW	7	85,270,476 (GRCm39)	missense	probably damaging	0.99
R1719:Vmn2r71	UTSW	7	85,270,435 (GRCm39)	missense	probably damaging	1.00
R1860:Vmn2r71	UTSW	7	85,264,782 (GRCm39)	missense	probably damaging	1.00
R2013:Vmn2r71	UTSW	7	85,269,845 (GRCm39)	missense	probably benign	0.38
R2014:Vmn2r71	UTSW	7	85,269,845 (GRCm39)	missense	probably benign	0.38
R2015:Vmn2r71	UTSW	7	85,269,845 (GRCm39)	missense	probably benign	0.38
R2050:Vmn2r71	UTSW	7	85,273,681 (GRCm39)	missense	probably damaging	1.00
R2084:Vmn2r71	UTSW	7	85,267,945 (GRCm39)	missense	probably benign	0.03
R2221:Vmn2r71	UTSW	7	85,273,301 (GRCm39)	missense	probably benign	0.40
R2223:Vmn2r71	UTSW	7	85,273,301 (GRCm39)	missense	probably benign	0.40
R2245:Vmn2r71	UTSW	7	85,273,388 (GRCm39)	missense	probably damaging	1.00
R3115:Vmn2r71	UTSW	7	85,272,866 (GRCm39)	missense	probably damaging	0.97
R3122:Vmn2r71	UTSW	7	85,264,828 (GRCm39)	nonsense	probably null
R3609:Vmn2r71	UTSW	7	85,268,870 (GRCm39)	missense	probably damaging	1.00
R4093:Vmn2r71	UTSW	7	85,270,442 (GRCm39)	missense	probably benign	0.00
R4305:Vmn2r71	UTSW	7	85,273,360 (GRCm39)	missense	probably damaging	1.00
R4306:Vmn2r71	UTSW	7	85,273,360 (GRCm39)	missense	probably damaging	1.00
R4334:Vmn2r71	UTSW	7	85,269,042 (GRCm39)	missense	probably benign	0.01
R4569:Vmn2r71	UTSW	7	85,273,402 (GRCm39)	missense	possibly damaging	0.66
R4622:Vmn2r71	UTSW	7	85,269,817 (GRCm39)	missense	probably benign	0.00
R4915:Vmn2r71	UTSW	7	85,270,476 (GRCm39)	missense	probably damaging	0.99
R4956:Vmn2r71	UTSW	7	85,268,436 (GRCm39)	missense	probably benign	0.19
R5005:Vmn2r71	UTSW	7	85,273,352 (GRCm39)	missense	probably damaging	1.00
R5045:Vmn2r71	UTSW	7	85,273,597 (GRCm39)	missense	probably benign	0.00
R5153:Vmn2r71	UTSW	7	85,268,430 (GRCm39)	missense	possibly damaging	0.94
R5236:Vmn2r71	UTSW	7	85,272,877 (GRCm39)	missense	probably damaging	1.00
R5373:Vmn2r71	UTSW	7	85,267,750 (GRCm39)	missense	possibly damaging	0.79
R5405:Vmn2r71	UTSW	7	85,268,622 (GRCm39)	missense	probably benign
R5831:Vmn2r71	UTSW	7	85,272,922 (GRCm39)	missense	probably benign	0.16
R6061:Vmn2r71	UTSW	7	85,268,482 (GRCm39)	missense	probably benign
R6518:Vmn2r71	UTSW	7	85,270,436 (GRCm39)	missense	probably damaging	1.00
R6751:Vmn2r71	UTSW	7	85,269,095 (GRCm39)	critical splice donor site	probably null
R6920:Vmn2r71	UTSW	7	85,273,108 (GRCm39)	missense	probably damaging	1.00
R7358:Vmn2r71	UTSW	7	85,273,468 (GRCm39)	missense	possibly damaging	0.81
R7453:Vmn2r71	UTSW	7	85,273,297 (GRCm39)	missense	probably benign	0.21
R7560:Vmn2r71	UTSW	7	85,273,115 (GRCm39)	missense	probably benign	0.06
R7871:Vmn2r71	UTSW	7	85,272,869 (GRCm39)	missense	possibly damaging	0.81
R8267:Vmn2r71	UTSW	7	85,264,704 (GRCm39)	missense	probably benign	0.02
R8377:Vmn2r71	UTSW	7	85,264,707 (GRCm39)	missense	probably benign
R9278:Vmn2r71	UTSW	7	85,269,788 (GRCm39)	missense	probably benign	0.19
R9319:Vmn2r71	UTSW	7	85,273,694 (GRCm39)	missense	probably damaging	1.00
R9329:Vmn2r71	UTSW	7	85,267,950 (GRCm39)	missense	probably benign	0.00
R9368:Vmn2r71	UTSW	7	85,273,442 (GRCm39)	missense	probably damaging	1.00
R9636:Vmn2r71	UTSW	7	85,268,388 (GRCm39)	missense	possibly damaging	0.80
R9756:Vmn2r71	UTSW	7	85,268,573 (GRCm39)	nonsense	probably null
X0025:Vmn2r71	UTSW	7	85,267,873 (GRCm39)	missense	probably benign
Z1186:Vmn2r71	UTSW	7	85,273,094 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGATCAAATGCTATGCAGCCTCCAC -3'
(R):5'- GGAGAAGTTCCAAGCCAGATTCCAC -3'

Sequencing Primer
(F):5'- GCAGCCTCCACACTTAAATTT -3'
(R):5'- GCAAATGTGATCTGCTGCAC -3'

Posted On

2013-11-07