Mutagenetix > Incidental Mutation

Incidental Mutation 'R0940:Or5p66'

81408

Institutional Source

Beutler Lab

Gene Symbol

Or5p66

Ensembl Gene

ENSMUSG00000109884

Gene Name

olfactory receptor family 5 subfamily P member 66

Synonyms

Olfr490, GA_x6K02T2PBJ9-10617173-10616229, MOR204-17

MMRRC Submission

039079-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.160) question?

Stock #

R0940 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

107885387-107886331 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 107886264 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 23 (D23G)

Ref Sequence

ENSEMBL: ENSMUSP00000147922 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000210114] [ENSMUST00000211345]

AlphaFold

Q8VFD2

Predicted Effect

probably benign
Transcript: ENSMUST00000074550
AA Change: D23G

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000074137
Gene: ENSMUSG00000093808
AA Change: D23G

Domain	Start	End	E-Value	Type
Pfam:7tm_4	34	311	4e-53	PFAM
Pfam:7tm_1	44	293	5e-23	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000210114
AA Change: D23G

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

Predicted Effect

probably benign
Transcript: ENSMUST00000211345
AA Change: D23G

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

Meta Mutation Damage Score

0.4804

Coding Region Coverage

1x: 99.5%
3x: 99.1%
10x: 98.0%
20x: 96.7%

Validation Efficiency

100% (68/68)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1810064F22Rik	C	T	9: 22,119,367 (GRCm39)		noncoding transcript	Het
2610021A01Rik	T	G	7: 41,275,858 (GRCm39)	I520M	probably damaging	Het
Ackr4	A	G	9: 103,976,831 (GRCm39)	F39L	probably damaging	Het
Adgre5	C	T	8: 84,460,126 (GRCm39)	S92N	probably damaging	Het
Adrb2	T	C	18: 62,312,762 (GRCm39)	D21G	probably benign	Het
Akr1c6	G	A	13: 4,486,372 (GRCm39)	E60K	probably benign	Het
Bcl7c	T	A	7: 127,306,503 (GRCm39)	N96I	possibly damaging	Het
Brca1	A	T	11: 101,422,969 (GRCm39)	S106R	possibly damaging	Het
C6	A	T	15: 4,764,717 (GRCm39)	T138S	probably benign	Het
Cul3	T	C	1: 80,300,564 (GRCm39)		probably benign	Het
Dnah8	T	A	17: 31,022,217 (GRCm39)	M3939K	probably damaging	Het
Dock4	T	A	12: 40,681,626 (GRCm39)		probably benign	Het
Dsc2	T	G	18: 20,183,116 (GRCm39)	T101P	probably damaging	Het
Dynlt1b	T	C	17: 6,697,649 (GRCm39)		probably benign	Het
E330013P04Rik	A	G	19: 60,150,354 (GRCm39)		noncoding transcript	Het
Fggy	A	G	4: 95,585,238 (GRCm39)	E39G	probably benign	Het
Fhip1a	A	G	3: 85,572,797 (GRCm39)	V952A	possibly damaging	Het
Fmo6	A	T	1: 162,753,795 (GRCm39)	C116S	probably benign	Het
Gadd45a	A	G	6: 67,013,813 (GRCm39)	I44T	possibly damaging	Het
Gmps	A	G	3: 63,883,743 (GRCm39)		probably benign	Het
Gnmt	A	G	17: 47,037,271 (GRCm39)	L171P	probably damaging	Het
Hnrnpm	G	A	17: 33,868,976 (GRCm39)	R523C	probably damaging	Het
Inpp5a	T	C	7: 139,105,654 (GRCm39)	Y202H	probably damaging	Het
Kank3	C	T	17: 34,036,450 (GRCm39)	S106F	probably damaging	Het
Lmcd1	A	G	6: 112,305,658 (GRCm39)	D253G	probably benign	Het
Lrrk2	A	T	15: 91,613,284 (GRCm39)	I803F	possibly damaging	Het
Mybpc2	T	C	7: 44,156,311 (GRCm39)	K834R	probably benign	Het
Mycbp2	A	T	14: 103,500,129 (GRCm39)		probably benign	Het
Myh4	A	T	11: 67,133,689 (GRCm39)	N243Y	probably damaging	Het
Myorg	A	G	4: 41,497,996 (GRCm39)	Y545H	probably damaging	Het
Nfatc1	C	T	18: 80,679,110 (GRCm39)	M759I	probably benign	Het
Nipal4	A	G	11: 46,041,139 (GRCm39)	I352T	possibly damaging	Het
Nomo1	A	G	7: 45,683,329 (GRCm39)	E25G	possibly damaging	Het
Or10j27	A	G	1: 172,958,020 (GRCm39)	S255P	probably benign	Het
Or13a19	T	C	7: 139,903,065 (GRCm39)	I151T	probably benign	Het
Or14j7	A	G	17: 38,234,591 (GRCm39)	I45V	probably damaging	Het
Or1e32	T	C	11: 73,705,050 (GRCm39)	N286S	probably damaging	Het
Or4c116	T	A	2: 88,942,419 (GRCm39)	I146L	probably benign	Het
Pabpn1l	A	G	8: 123,349,183 (GRCm39)	V78A	probably benign	Het
Pde6b	T	A	5: 108,568,203 (GRCm39)	I327N	possibly damaging	Het
Phrf1	T	C	7: 140,834,768 (GRCm39)		probably benign	Het
Pkm	C	T	9: 59,575,818 (GRCm39)		probably benign	Het
Plxna2	G	A	1: 194,482,863 (GRCm39)	V1519I	probably benign	Het
Ppp2cb	T	C	8: 34,105,689 (GRCm39)		probably null	Het
Prickle2	A	G	6: 92,387,984 (GRCm39)	Y473H	probably benign	Het
Prpf3	A	G	3: 95,751,535 (GRCm39)	W389R	probably damaging	Het
Psme4	G	A	11: 30,765,264 (GRCm39)	E544K	possibly damaging	Het
Relb	A	T	7: 19,345,767 (GRCm39)	D395E	probably damaging	Het
Rif1	GCCACCA	GCCA	2: 52,000,336 (GRCm39)		probably benign	Het
Rnf213	A	G	11: 119,307,389 (GRCm39)	N683S	probably benign	Het
Rtel1	T	C	2: 180,964,596 (GRCm39)	C102R	probably benign	Het
Sel1l3	C	T	5: 53,301,379 (GRCm39)		probably benign	Het
Slc8a2	A	G	7: 15,878,887 (GRCm39)	T458A	probably benign	Het
Smc3	T	A	19: 53,629,340 (GRCm39)	M931K	probably benign	Het
Sorbs2	T	C	8: 46,249,539 (GRCm39)	V795A	probably benign	Het
Tgm3	A	G	2: 129,854,326 (GRCm39)	S2G	probably benign	Het
Tpbpa	T	C	13: 61,087,867 (GRCm39)	T75A	probably damaging	Het
Trub1	A	G	19: 57,473,495 (GRCm39)		probably benign	Het
Uggt2	A	G	14: 119,328,604 (GRCm39)		probably null	Het
Ugt2a3	A	G	5: 87,475,065 (GRCm39)	V393A	possibly damaging	Het
Vav3	T	A	3: 109,470,151 (GRCm39)	M532K	possibly damaging	Het
Zfp616	A	T	11: 73,975,850 (GRCm39)	K706N	probably damaging	Het
Zkscan1	T	C	5: 138,091,432 (GRCm39)	F55S	probably damaging	Het

Other mutations in Or5p66

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0395:Or5p66	UTSW	7	107,885,478 (GRCm39)	missense	probably benign	0.00
R0634:Or5p66	UTSW	7	107,885,503 (GRCm39)	missense	probably benign	0.00
R1990:Or5p66	UTSW	7	107,885,566 (GRCm39)	nonsense	probably null
R1991:Or5p66	UTSW	7	107,885,566 (GRCm39)	nonsense	probably null
R2860:Or5p66	UTSW	7	107,886,169 (GRCm39)	missense	probably damaging	0.98
R2861:Or5p66	UTSW	7	107,886,169 (GRCm39)	missense	probably damaging	0.98
R3712:Or5p66	UTSW	7	107,885,663 (GRCm39)	nonsense	probably null
R4735:Or5p66	UTSW	7	107,885,520 (GRCm39)	missense	probably benign
R4895:Or5p66	UTSW	7	107,885,802 (GRCm39)	missense	probably damaging	0.97
R4976:Or5p66	UTSW	7	107,885,818 (GRCm39)	missense	probably damaging	1.00
R5686:Or5p66	UTSW	7	107,885,949 (GRCm39)	missense	probably damaging	1.00
R5719:Or5p66	UTSW	7	107,885,599 (GRCm39)	missense	probably damaging	0.97
R5883:Or5p66	UTSW	7	107,885,451 (GRCm39)	missense	probably damaging	1.00
R5911:Or5p66	UTSW	7	107,885,605 (GRCm39)	missense	probably damaging	1.00
R6452:Or5p66	UTSW	7	107,886,100 (GRCm39)	missense	probably damaging	1.00
R7018:Or5p66	UTSW	7	107,885,551 (GRCm39)	missense	probably benign	0.34
R7233:Or5p66	UTSW	7	107,885,923 (GRCm39)	missense	probably benign	0.36
R8085:Or5p66	UTSW	7	107,885,620 (GRCm39)	missense	probably benign	0.01
R8166:Or5p66	UTSW	7	107,885,904 (GRCm39)	missense	probably benign	0.06
R8919:Or5p66	UTSW	7	107,886,289 (GRCm39)	missense	probably damaging	1.00
R9209:Or5p66	UTSW	7	107,885,526 (GRCm39)	missense	probably benign	0.06
R9612:Or5p66	UTSW	7	107,885,487 (GRCm39)	missense	probably benign
R9789:Or5p66	UTSW	7	107,885,898 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- GTCCACagaagagaagagaaaagaaggaaa -3'
(R):5'- ATCCAATCTAGCACCCCAATTACATGTTT -3'

Sequencing Primer
(F):5'- agaaaagaaggaaagtaaaaaggagg -3'
(R):5'- GCACCCCAATTACATGTTTTAAATG -3'

Posted On

2013-11-07