Incidental Mutation 'R0879:Zfp865'
| ID |
81918 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp865
|
| Ensembl Gene |
ENSMUSG00000116184 |
| Gene Name |
zinc finger protein 865 |
| Synonyms |
6430526N21Rik |
| MMRRC Submission |
039046-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.123)
|
| Stock # |
R0879 (G1)
|
| Quality Score |
225 |
|
Status
|
Validated
|
| Chromosome |
7 |
| Chromosomal Location |
5023375-5036225 bp(+) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to G
at 5034342 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Threonine to Alanine
at position 776
(T776A)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000075601
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000062428]
[ENSMUST00000076251]
[ENSMUST00000076791]
[ENSMUST00000085427]
[ENSMUST00000207050]
[ENSMUST00000208728]
|
| AlphaFold |
Q9D656 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000062428
|
| SMART Domains |
Protein: ENSMUSP00000051979
Gene: ENSMUSG00000043290
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
8 |
24 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
64 |
86 |
1.02e1 |
SMART |
|
ZnF_C2H2
|
100 |
122 |
9.96e-1 |
SMART |
|
ZnF_C2H2
|
128 |
150 |
6.67e-2 |
SMART |
|
low complexity region
|
180 |
192 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
195 |
217 |
4.11e-2 |
SMART |
|
ZnF_C2H2
|
223 |
245 |
7.26e-3 |
SMART |
|
ZnF_C2H2
|
251 |
273 |
3.21e-4 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000076251
AA Change: T776A
PolyPhen 2
Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
|
| SMART Domains |
Protein: ENSMUSP00000075601
Gene: ENSMUSG00000074405
AA Change: T776A
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
6 |
20 |
N/A |
INTRINSIC |
|
low complexity region
|
62 |
82 |
N/A |
INTRINSIC |
|
low complexity region
|
120 |
141 |
N/A |
INTRINSIC |
|
low complexity region
|
171 |
193 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
220 |
242 |
1.28e-3 |
SMART |
|
ZnF_C2H2
|
248 |
270 |
5.5e-3 |
SMART |
|
low complexity region
|
274 |
286 |
N/A |
INTRINSIC |
|
low complexity region
|
294 |
324 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
350 |
372 |
8.81e-2 |
SMART |
|
ZnF_C2H2
|
378 |
400 |
6.08e-5 |
SMART |
|
ZnF_C2H2
|
407 |
429 |
1.79e-2 |
SMART |
|
ZnF_C2H2
|
439 |
461 |
1.92e-2 |
SMART |
|
low complexity region
|
478 |
495 |
N/A |
INTRINSIC |
|
low complexity region
|
502 |
516 |
N/A |
INTRINSIC |
|
low complexity region
|
523 |
542 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
546 |
568 |
4.47e-3 |
SMART |
|
ZnF_C2H2
|
574 |
596 |
5.42e-2 |
SMART |
|
ZnF_C2H2
|
602 |
624 |
1.72e-4 |
SMART |
|
low complexity region
|
628 |
660 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
664 |
686 |
5.34e-1 |
SMART |
|
ZnF_C2H2
|
692 |
714 |
2.82e0 |
SMART |
|
low complexity region
|
725 |
745 |
N/A |
INTRINSIC |
|
low complexity region
|
750 |
770 |
N/A |
INTRINSIC |
|
low complexity region
|
772 |
788 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
791 |
813 |
1.64e-1 |
SMART |
|
ZnF_C2H2
|
819 |
841 |
9.3e-1 |
SMART |
|
ZnF_C2H2
|
847 |
869 |
2.95e-3 |
SMART |
|
ZnF_C2H2
|
875 |
897 |
3.83e-2 |
SMART |
|
ZnF_C2H2
|
903 |
925 |
2.05e-2 |
SMART |
|
ZnF_C2H2
|
931 |
953 |
1.18e-2 |
SMART |
|
ZnF_C2H2
|
959 |
981 |
1.36e-2 |
SMART |
|
ZnF_C2H2
|
988 |
1010 |
5.06e-2 |
SMART |
|
ZnF_C2H2
|
1016 |
1038 |
4.72e-2 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000076791
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000085427
|
| SMART Domains |
Protein: ENSMUSP00000082550
Gene: ENSMUSG00000074405
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
6 |
20 |
N/A |
INTRINSIC |
|
low complexity region
|
62 |
82 |
N/A |
INTRINSIC |
|
low complexity region
|
120 |
141 |
N/A |
INTRINSIC |
|
low complexity region
|
171 |
193 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
220 |
242 |
1.28e-3 |
SMART |
|
ZnF_C2H2
|
248 |
270 |
5.5e-3 |
SMART |
|
low complexity region
|
274 |
286 |
N/A |
INTRINSIC |
|
low complexity region
|
294 |
324 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
350 |
372 |
8.81e-2 |
SMART |
|
ZnF_C2H2
|
378 |
400 |
6.08e-5 |
SMART |
|
ZnF_C2H2
|
407 |
429 |
1.79e-2 |
SMART |
|
ZnF_C2H2
|
439 |
461 |
1.92e-2 |
SMART |
|
low complexity region
|
478 |
495 |
N/A |
INTRINSIC |
|
low complexity region
|
502 |
516 |
N/A |
INTRINSIC |
|
low complexity region
|
523 |
542 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
546 |
568 |
4.47e-3 |
SMART |
|
ZnF_C2H2
|
574 |
596 |
5.42e-2 |
SMART |
|
ZnF_C2H2
|
602 |
624 |
1.72e-4 |
SMART |
|
low complexity region
|
628 |
660 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
664 |
686 |
5.34e-1 |
SMART |
|
ZnF_C2H2
|
692 |
714 |
2.82e0 |
SMART |
|
low complexity region
|
725 |
745 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000207050
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000207193
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000207362
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000208728
|
| Meta Mutation Damage Score |
0.0898 |
| Coding Region Coverage |
- 1x: 99.5%
- 3x: 99.0%
- 10x: 97.9%
- 20x: 96.3%
|
| Validation Efficiency |
100% (41/41) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 40 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Aagab |
T |
A |
9: 63,524,892 (GRCm39) |
|
probably benign |
Het |
| Adm |
A |
G |
7: 110,227,559 (GRCm39) |
D25G |
possibly damaging |
Het |
| Adprs |
C |
T |
4: 126,210,410 (GRCm39) |
V357I |
probably benign |
Het |
| Akap6 |
G |
T |
12: 52,927,582 (GRCm39) |
R164L |
probably damaging |
Het |
| Baz2a |
A |
G |
10: 127,957,173 (GRCm39) |
N972S |
probably damaging |
Het |
| Brd2 |
A |
T |
17: 34,332,420 (GRCm39) |
V232D |
probably benign |
Het |
| C6 |
A |
G |
15: 4,792,818 (GRCm39) |
|
probably benign |
Het |
| Ceacam5 |
A |
C |
7: 17,491,627 (GRCm39) |
I666L |
probably benign |
Het |
| Col7a1 |
T |
C |
9: 108,805,159 (GRCm39) |
|
probably benign |
Het |
| Dnah17 |
C |
T |
11: 117,947,661 (GRCm39) |
|
probably benign |
Het |
| Dnah7a |
A |
T |
1: 53,467,019 (GRCm39) |
V3615E |
possibly damaging |
Het |
| Eml6 |
A |
G |
11: 29,800,816 (GRCm39) |
|
probably null |
Het |
| Enpp2 |
T |
C |
15: 54,741,326 (GRCm39) |
E324G |
probably damaging |
Het |
| Fgd4 |
A |
G |
16: 16,295,313 (GRCm39) |
V222A |
probably damaging |
Het |
| Gm4076 |
A |
G |
13: 85,275,326 (GRCm39) |
|
noncoding transcript |
Het |
| Gm4775 |
T |
C |
14: 106,338,227 (GRCm39) |
|
noncoding transcript |
Het |
| Igsf9b |
T |
C |
9: 27,245,038 (GRCm39) |
S1002P |
probably damaging |
Het |
| Jag1 |
A |
G |
2: 136,942,001 (GRCm39) |
S244P |
possibly damaging |
Het |
| Klhl41 |
A |
T |
2: 69,513,827 (GRCm39) |
|
probably benign |
Het |
| Ltbr |
A |
G |
6: 125,290,338 (GRCm39) |
|
probably benign |
Het |
| Megf8 |
A |
G |
7: 25,037,896 (GRCm39) |
E804G |
possibly damaging |
Het |
| Mybpc1 |
G |
A |
10: 88,407,378 (GRCm39) |
|
probably benign |
Het |
| Npas4 |
T |
C |
19: 5,036,944 (GRCm39) |
R407G |
probably benign |
Het |
| Oxnad1 |
T |
A |
14: 31,821,553 (GRCm39) |
Y213N |
probably damaging |
Het |
| Pde6d |
A |
G |
1: 86,473,523 (GRCm39) |
F91S |
probably benign |
Het |
| Pelp1 |
A |
G |
11: 70,286,123 (GRCm39) |
|
probably benign |
Het |
| Plscr2 |
T |
C |
9: 92,169,846 (GRCm39) |
Y99H |
probably damaging |
Het |
| Rft1 |
T |
C |
14: 30,404,705 (GRCm39) |
|
probably benign |
Het |
| Ryr3 |
T |
C |
2: 112,860,588 (GRCm39) |
Y30C |
probably benign |
Het |
| Selenbp2 |
A |
G |
3: 94,606,863 (GRCm39) |
T108A |
possibly damaging |
Het |
| Stk32b |
A |
G |
5: 37,616,940 (GRCm39) |
|
probably benign |
Het |
| Stra6 |
T |
C |
9: 58,042,487 (GRCm39) |
|
probably null |
Het |
| Usp17le |
A |
T |
7: 104,418,854 (GRCm39) |
L96Q |
probably damaging |
Het |
| Usp17le |
G |
T |
7: 104,418,855 (GRCm39) |
L96M |
possibly damaging |
Het |
| Vmn1r76 |
A |
T |
7: 11,664,662 (GRCm39) |
I184N |
probably benign |
Het |
| Vmn2r102 |
T |
C |
17: 19,914,454 (GRCm39) |
V673A |
probably damaging |
Het |
| Wdr17 |
T |
G |
8: 55,114,516 (GRCm39) |
I667L |
probably benign |
Het |
| Zfp292 |
T |
C |
4: 34,811,218 (GRCm39) |
T609A |
probably benign |
Het |
| Zfp821 |
T |
C |
8: 110,448,474 (GRCm39) |
I135T |
possibly damaging |
Het |
| Zp2 |
G |
T |
7: 119,734,757 (GRCm39) |
P477Q |
probably damaging |
Het |
|
| Other mutations in Zfp865 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01731:Zfp865
|
APN |
7 |
5,032,875 (GRCm39) |
missense |
probably benign |
|
|
IGL02041:Zfp865
|
APN |
7 |
5,034,372 (GRCm39) |
missense |
probably benign |
|
|
IGL03118:Zfp865
|
APN |
7 |
5,037,644 (GRCm39) |
intron |
probably benign |
|
|
R0613:Zfp865
|
UTSW |
7 |
5,032,090 (GRCm39) |
missense |
possibly damaging |
0.86 |
|
R0938:Zfp865
|
UTSW |
7 |
5,034,403 (GRCm39) |
missense |
possibly damaging |
0.96 |
|
R1448:Zfp865
|
UTSW |
7 |
5,032,278 (GRCm39) |
nonsense |
probably null |
|
|
R3955:Zfp865
|
UTSW |
7 |
5,035,013 (GRCm39) |
missense |
probably damaging |
0.96 |
|
R4841:Zfp865
|
UTSW |
7 |
5,034,640 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4842:Zfp865
|
UTSW |
7 |
5,034,640 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5044:Zfp865
|
UTSW |
7 |
5,037,668 (GRCm39) |
intron |
probably benign |
|
|
R5773:Zfp865
|
UTSW |
7 |
5,037,693 (GRCm39) |
intron |
probably benign |
|
|
R5843:Zfp865
|
UTSW |
7 |
5,033,416 (GRCm39) |
missense |
probably benign |
0.03 |
|
R5849:Zfp865
|
UTSW |
7 |
5,034,086 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6393:Zfp865
|
UTSW |
7 |
5,033,065 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6480:Zfp865
|
UTSW |
7 |
5,032,782 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R6681:Zfp865
|
UTSW |
7 |
5,032,450 (GRCm39) |
missense |
possibly damaging |
0.86 |
|
R6880:Zfp865
|
UTSW |
7 |
5,033,548 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7252:Zfp865
|
UTSW |
7 |
5,037,416 (GRCm39) |
intron |
probably benign |
|
|
R7302:Zfp865
|
UTSW |
7 |
5,032,252 (GRCm39) |
missense |
possibly damaging |
0.96 |
|
R7486:Zfp865
|
UTSW |
7 |
5,034,259 (GRCm39) |
missense |
possibly damaging |
0.85 |
|
R7611:Zfp865
|
UTSW |
7 |
5,034,130 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R8058:Zfp865
|
UTSW |
7 |
5,033,445 (GRCm39) |
missense |
probably benign |
|
|
R8327:Zfp865
|
UTSW |
7 |
5,034,058 (GRCm39) |
missense |
probably benign |
0.08 |
|
R8728:Zfp865
|
UTSW |
7 |
5,034,819 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R9650:Zfp865
|
UTSW |
7 |
5,037,683 (GRCm39) |
missense |
unknown |
|
|
| Predicted Primers |
PCR Primer
(F):5'- ATGTGGCACAAGCTGGTTCACC -3'
(R):5'- AGCTCAGGTAGAAGAAGCCCTTGC -3'
Sequencing Primer
(F):5'- AAGCTGGTTCACCAGGCTG -3'
(R):5'- TCCGCTAGGCTAGAAGACTG -3'
|
| Posted On |
2013-11-08 |
Incidental Mutation