Mutagenetix > Incidental Mutation

Incidental Mutation 'R0863:Ralgapa1'

82213

Institutional Source

Beutler Lab

Gene Symbol

Ralgapa1

Ensembl Gene

ENSMUSG00000021027

Gene Name

Ral GTPase activating protein, alpha subunit 1

Synonyms

Garnl1, 4930400K19Rik, 2310003F20Rik, Tulip1

MMRRC Submission

039037-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.755) question?

Stock #

R0863 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

55602896-55821167 bp(-) (GRCm38)

Type of Mutation

splice site

DNA Base Change (assembly)

C to A at 55782777 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000154749 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000085385] [ENSMUST00000110687] [ENSMUST00000219432] [ENSMUST00000220367] [ENSMUST00000226244]

AlphaFold

Q6GYP7

Predicted Effect

probably benign
Transcript: ENSMUST00000085385

SMART Domains

Protein: ENSMUSP00000082503
Gene: ENSMUSG00000021027

Domain	Start	End	E-Value	Type
low complexity region	644	651	N/A	INTRINSIC
low complexity region	676	690	N/A	INTRINSIC
low complexity region	692	704	N/A	INTRINSIC
low complexity region	894	915	N/A	INTRINSIC
low complexity region	1386	1395	N/A	INTRINSIC
low complexity region	1784	1798	N/A	INTRINSIC
Pfam:Rap_GAP	1824	2003	7.4e-66	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000110687

SMART Domains

Protein: ENSMUSP00000106315
Gene: ENSMUSG00000021027

Domain	Start	End	E-Value	Type
low complexity region	644	651	N/A	INTRINSIC
low complexity region	676	690	N/A	INTRINSIC
low complexity region	692	704	N/A	INTRINSIC
low complexity region	894	915	N/A	INTRINSIC
low complexity region	1386	1395	N/A	INTRINSIC
low complexity region	1784	1798	N/A	INTRINSIC
Pfam:Rap_GAP	1824	2001	1.9e-48	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000219432

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000219529

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000219542

Predicted Effect

probably benign
Transcript: ENSMUST00000220367

Predicted Effect

probably benign
Transcript: ENSMUST00000226244

Coding Region Coverage

1x: 99.5%
3x: 98.9%
10x: 97.4%
20x: 94.8%

Validation Efficiency

94% (59/63)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a major subunit of the RAL-GTPase activating protein. A similar protein in mouse binds E12, a transcriptional regulator of immunoglobulin genes. The mouse protein also functions in skeletal muscle by binding to the regulatory 14-3-3 proteins upon stimulation with insulin or muscle contraction. A pseudogene of this gene has been identified on chromosome 9. [provided by RefSeq, Oct 2016]

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700057G04Rik	A	T	9: 92,351,087 (GRCm38)	I88L	possibly damaging	Het
2310016G11Rik	A	G	7: 44,677,808 (GRCm38)		noncoding transcript	Het
4930433I11Rik	A	T	7: 40,993,056 (GRCm38)	T141S	probably benign	Het
Abca14	A	T	7: 120,216,230 (GRCm38)	T234S	probably benign	Het
Acap1	C	A	11: 69,887,056 (GRCm38)	V119L	probably damaging	Het
Actr2	C	T	11: 20,080,760 (GRCm38)	V163I	probably benign	Het
Adcy4	T	C	14: 55,783,599 (GRCm38)	Y27C	probably damaging	Het
Arnt2	T	A	7: 84,265,584 (GRCm38)	K524M	probably damaging	Het
Brca1	T	C	11: 101,524,770 (GRCm38)	Y846C	probably benign	Het
Capn7	T	A	14: 31,369,757 (GRCm38)	C704S	possibly damaging	Het
Cep350	T	A	1: 155,862,235 (GRCm38)	I2621L	probably benign	Het
Col11a1	G	A	3: 114,138,765 (GRCm38)	R113H	unknown	Het
Ctnnbl1	C	T	2: 157,799,417 (GRCm38)		probably benign	Het
Cul9	T	C	17: 46,537,822 (GRCm38)		probably null	Het
Erc2	A	C	14: 28,025,148 (GRCm38)	N345T	probably benign	Het
Fank1	A	G	7: 133,880,623 (GRCm38)	R73G	possibly damaging	Het
Fes	A	T	7: 80,380,886 (GRCm38)	W552R	probably damaging	Het
Fsd2	A	G	7: 81,542,165 (GRCm38)	V488A	possibly damaging	Het
Gfm1	T	C	3: 67,474,595 (GRCm38)	S705P	probably damaging	Het
Gm9493	A	T	19: 23,619,809 (GRCm38)	Q23L	probably benign	Het
Gucy1b2	T	C	14: 62,419,062 (GRCm38)	D282G	probably benign	Het
H2-Ab1	T	C	17: 34,267,354 (GRCm38)	I129T	probably damaging	Het
H2-M10.3	T	A	17: 36,366,690 (GRCm38)	Y232F	probably damaging	Het
Iqca	C	A	1: 90,142,731 (GRCm38)	G133V	probably null	Het
Lonp1	T	C	17: 56,618,331 (GRCm38)	K487R	probably damaging	Het
Ltbp1	A	G	17: 75,252,386 (GRCm38)	Y290C	probably damaging	Het
Mgea5	C	T	19: 45,782,986 (GRCm38)	A49T	probably benign	Het
Ms4a10	C	T	19: 10,968,593 (GRCm38)	G58D	probably damaging	Het
Muc5b	A	G	7: 141,867,717 (GRCm38)	S4315G	probably benign	Het
Nlrp1b	T	A	11: 71,181,347 (GRCm38)	T557S	probably benign	Het
Nlrp3	A	G	11: 59,565,850 (GRCm38)	D946G	probably benign	Het
Obscn	T	C	11: 58,995,415 (GRCm38)		probably benign	Het
Olfr469	T	C	7: 107,823,374 (GRCm38)	S32G	probably benign	Het
Olfr509	A	G	7: 108,645,658 (GRCm38)	I306T	probably benign	Het
Olfr866	A	T	9: 20,027,213 (GRCm38)	S242T	probably damaging	Het
Pask	A	T	1: 93,314,339 (GRCm38)	F1219I	probably damaging	Het
Pcdhb2	G	A	18: 37,295,657 (GRCm38)	V228I	possibly damaging	Het
Phf1	T	C	17: 26,937,140 (GRCm38)		probably benign	Het
Plec	G	A	15: 76,174,080 (GRCm38)	Q3751*	probably null	Het
Ppp1r12c	G	T	7: 4,486,366 (GRCm38)	Q240K	probably damaging	Het
Scel	T	A	14: 103,586,480 (GRCm38)	S381R	possibly damaging	Het
Sema4a	C	T	3: 88,448,149 (GRCm38)		probably benign	Het
Sltm	A	G	9: 70,561,908 (GRCm38)	T150A	probably benign	Het
Spag1	G	T	15: 36,192,047 (GRCm38)	K217N	probably damaging	Het
Ssh1	C	T	5: 113,966,731 (GRCm38)	R9H	probably damaging	Het
St3gal4	C	A	9: 35,053,448 (GRCm38)	V155F	probably damaging	Het
Stxbp5	C	T	10: 9,809,040 (GRCm38)	E539K	possibly damaging	Het
Tbc1d7	G	T	13: 43,154,685 (GRCm38)		probably benign	Het
Thnsl2	A	T	6: 71,134,224 (GRCm38)	L220*	probably null	Het
Tinf2	G	A	14: 55,680,109 (GRCm38)	P308S	probably benign	Het
Tnf	T	C	17: 35,201,144 (GRCm38)		probably benign	Het
Ttc21b	T	C	2: 66,242,773 (GRCm38)	I190V	probably benign	Het
Ttn	T	C	2: 76,707,047 (GRCm38)	T34846A	probably benign	Het
Ube4a	A	G	9: 44,949,816 (GRCm38)	V232A	possibly damaging	Het
Uri1	A	T	7: 37,969,675 (GRCm38)	D122E	probably damaging	Het
Vmn2r94	T	G	17: 18,257,711 (GRCm38)	Q146P	probably damaging	Het
Zan	T	A	5: 137,458,639 (GRCm38)	E1278D	unknown	Het
Zfhx4	T	A	3: 5,245,315 (GRCm38)	S919R	possibly damaging	Het

Other mutations in Ralgapa1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00089:Ralgapa1	APN	12	55,722,773 (GRCm38)	missense	probably damaging	0.98
IGL00494:Ralgapa1	APN	12	55,747,185 (GRCm38)	missense	probably damaging	1.00
IGL00731:Ralgapa1	APN	12	55,702,452 (GRCm38)	missense	possibly damaging	0.94
IGL00851:Ralgapa1	APN	12	55,709,575 (GRCm38)	missense	possibly damaging	0.93
IGL01133:Ralgapa1	APN	12	55,642,359 (GRCm38)	missense	probably damaging	0.99
IGL01133:Ralgapa1	APN	12	55,642,348 (GRCm38)	missense	probably damaging	1.00
IGL01354:Ralgapa1	APN	12	55,777,316 (GRCm38)	missense	possibly damaging	0.68
IGL01514:Ralgapa1	APN	12	55,719,657 (GRCm38)	missense	probably damaging	0.97
IGL02033:Ralgapa1	APN	12	55,642,477 (GRCm38)	missense	possibly damaging	0.69
IGL02064:Ralgapa1	APN	12	55,708,077 (GRCm38)	missense	probably damaging	1.00
IGL02556:Ralgapa1	APN	12	55,642,449 (GRCm38)	missense	possibly damaging	0.80
IGL02605:Ralgapa1	APN	12	55,712,665 (GRCm38)	missense	possibly damaging	0.90
IGL02657:Ralgapa1	APN	12	55,673,507 (GRCm38)	missense	probably damaging	1.00
IGL02676:Ralgapa1	APN	12	55,676,417 (GRCm38)	missense	probably damaging	1.00
IGL02894:Ralgapa1	APN	12	55,717,069 (GRCm38)	missense	possibly damaging	0.79
IGL02944:Ralgapa1	APN	12	55,757,951 (GRCm38)	missense	probably benign	0.01
Anhydrous	UTSW	12	55,795,778 (GRCm38)	critical splice acceptor site	probably null
Aqueous	UTSW	12	55,698,854 (GRCm38)	missense	probably damaging	1.00
bantam	UTSW	12	55,722,773 (GRCm38)	critical splice donor site	probably null
Deliquescent	UTSW	12	55,782,900 (GRCm38)	splice site	probably benign
wickedwarlock	UTSW	12	55,777,292 (GRCm38)	missense	probably null	0.99
F5770:Ralgapa1	UTSW	12	55,795,653 (GRCm38)	splice site	probably benign
IGL03046:Ralgapa1	UTSW	12	55,695,157 (GRCm38)	missense	probably damaging	1.00
R0011:Ralgapa1	UTSW	12	55,786,263 (GRCm38)	missense	probably damaging	0.99
R0096:Ralgapa1	UTSW	12	55,739,505 (GRCm38)	missense	probably damaging	1.00
R0277:Ralgapa1	UTSW	12	55,677,238 (GRCm38)	missense	probably damaging	0.99
R0323:Ralgapa1	UTSW	12	55,677,238 (GRCm38)	missense	probably damaging	0.99
R0333:Ralgapa1	UTSW	12	55,782,900 (GRCm38)	splice site	probably benign
R0361:Ralgapa1	UTSW	12	55,676,569 (GRCm38)	missense	possibly damaging	0.93
R0385:Ralgapa1	UTSW	12	55,677,038 (GRCm38)	missense	probably damaging	1.00
R0386:Ralgapa1	UTSW	12	55,708,067 (GRCm38)	missense	probably benign	0.03
R0498:Ralgapa1	UTSW	12	55,689,791 (GRCm38)	missense	possibly damaging	0.66
R0552:Ralgapa1	UTSW	12	55,676,765 (GRCm38)	missense	probably benign	0.27
R0564:Ralgapa1	UTSW	12	55,782,885 (GRCm38)	missense	possibly damaging	0.84
R0611:Ralgapa1	UTSW	12	55,795,698 (GRCm38)	missense	probably damaging	0.99
R0730:Ralgapa1	UTSW	12	55,665,663 (GRCm38)	missense	probably damaging	1.00
R0741:Ralgapa1	UTSW	12	55,676,581 (GRCm38)	missense	probably damaging	0.99
R0815:Ralgapa1	UTSW	12	55,782,777 (GRCm38)	splice site	probably benign
R0815:Ralgapa1	UTSW	12	55,762,681 (GRCm38)	nonsense	probably null
R0863:Ralgapa1	UTSW	12	55,762,681 (GRCm38)	nonsense	probably null
R1068:Ralgapa1	UTSW	12	55,790,310 (GRCm38)	critical splice donor site	probably null
R1147:Ralgapa1	UTSW	12	55,702,480 (GRCm38)	missense	probably damaging	1.00
R1147:Ralgapa1	UTSW	12	55,702,480 (GRCm38)	missense	probably damaging	1.00
R1256:Ralgapa1	UTSW	12	55,762,661 (GRCm38)	missense	possibly damaging	0.94
R1343:Ralgapa1	UTSW	12	55,707,978 (GRCm38)	missense	probably damaging	1.00
R1378:Ralgapa1	UTSW	12	55,676,926 (GRCm38)	missense	probably damaging	1.00
R1474:Ralgapa1	UTSW	12	55,741,480 (GRCm38)	missense	probably benign	0.09
R1494:Ralgapa1	UTSW	12	55,684,524 (GRCm38)	missense	probably damaging	0.99
R1593:Ralgapa1	UTSW	12	55,770,703 (GRCm38)	missense	probably damaging	1.00
R1607:Ralgapa1	UTSW	12	55,741,536 (GRCm38)	missense	probably damaging	1.00
R1681:Ralgapa1	UTSW	12	55,762,603 (GRCm38)	missense	probably benign	0.35
R1689:Ralgapa1	UTSW	12	55,676,767 (GRCm38)	missense	possibly damaging	0.79
R1714:Ralgapa1	UTSW	12	55,642,389 (GRCm38)	missense	probably damaging	1.00
R1832:Ralgapa1	UTSW	12	55,757,967 (GRCm38)	missense	probably benign	0.03
R1870:Ralgapa1	UTSW	12	55,677,032 (GRCm38)	missense	possibly damaging	0.66
R2040:Ralgapa1	UTSW	12	55,786,322 (GRCm38)	missense	probably damaging	1.00
R2043:Ralgapa1	UTSW	12	55,677,026 (GRCm38)	missense	probably damaging	0.99
R2046:Ralgapa1	UTSW	12	55,695,160 (GRCm38)	missense	probably damaging	1.00
R2109:Ralgapa1	UTSW	12	55,776,188 (GRCm38)	missense	possibly damaging	0.90
R2114:Ralgapa1	UTSW	12	55,786,349 (GRCm38)	critical splice acceptor site	probably null
R2115:Ralgapa1	UTSW	12	55,786,349 (GRCm38)	critical splice acceptor site	probably null
R2202:Ralgapa1	UTSW	12	55,612,800 (GRCm38)	splice site	probably null
R2203:Ralgapa1	UTSW	12	55,612,800 (GRCm38)	splice site	probably null
R2233:Ralgapa1	UTSW	12	55,717,071 (GRCm38)	missense	probably benign	0.13
R2235:Ralgapa1	UTSW	12	55,717,071 (GRCm38)	missense	probably benign	0.13
R2341:Ralgapa1	UTSW	12	55,677,124 (GRCm38)	missense	possibly damaging	0.66
R2507:Ralgapa1	UTSW	12	55,718,201 (GRCm38)	missense	probably damaging	1.00
R2508:Ralgapa1	UTSW	12	55,718,201 (GRCm38)	missense	probably damaging	1.00
R2972:Ralgapa1	UTSW	12	55,820,755 (GRCm38)	missense	possibly damaging	0.61
R3160:Ralgapa1	UTSW	12	55,709,586 (GRCm38)	missense	probably damaging	1.00
R3162:Ralgapa1	UTSW	12	55,709,586 (GRCm38)	missense	probably damaging	1.00
R3401:Ralgapa1	UTSW	12	55,659,137 (GRCm38)	missense	possibly damaging	0.66
R3416:Ralgapa1	UTSW	12	55,770,613 (GRCm38)	splice site	probably benign
R3499:Ralgapa1	UTSW	12	55,695,143 (GRCm38)	splice site	probably benign
R3799:Ralgapa1	UTSW	12	55,659,130 (GRCm38)	missense	probably damaging	1.00
R3948:Ralgapa1	UTSW	12	55,698,767 (GRCm38)	missense	probably damaging	1.00
R4039:Ralgapa1	UTSW	12	55,795,701 (GRCm38)	missense	probably damaging	0.99
R4120:Ralgapa1	UTSW	12	55,640,644 (GRCm38)	missense	probably damaging	1.00
R4165:Ralgapa1	UTSW	12	55,640,644 (GRCm38)	missense	probably damaging	1.00
R4166:Ralgapa1	UTSW	12	55,640,644 (GRCm38)	missense	probably damaging	1.00
R4212:Ralgapa1	UTSW	12	55,739,330 (GRCm38)	critical splice donor site	probably null
R4232:Ralgapa1	UTSW	12	55,640,644 (GRCm38)	missense	probably damaging	1.00
R4233:Ralgapa1	UTSW	12	55,640,644 (GRCm38)	missense	probably damaging	1.00
R4234:Ralgapa1	UTSW	12	55,640,644 (GRCm38)	missense	probably damaging	1.00
R4235:Ralgapa1	UTSW	12	55,640,644 (GRCm38)	missense	probably damaging	1.00
R4399:Ralgapa1	UTSW	12	55,795,778 (GRCm38)	critical splice acceptor site	probably null
R4698:Ralgapa1	UTSW	12	55,677,276 (GRCm38)	splice site	probably null
R4715:Ralgapa1	UTSW	12	55,693,458 (GRCm38)	missense	probably damaging	1.00
R4755:Ralgapa1	UTSW	12	55,712,748 (GRCm38)	missense	probably damaging	1.00
R4810:Ralgapa1	UTSW	12	55,794,993 (GRCm38)	critical splice donor site	probably null
R4827:Ralgapa1	UTSW	12	55,676,437 (GRCm38)	missense	probably damaging	1.00
R4849:Ralgapa1	UTSW	12	55,698,803 (GRCm38)	missense	probably damaging	0.99
R4934:Ralgapa1	UTSW	12	55,762,574 (GRCm38)	missense	possibly damaging	0.94
R5006:Ralgapa1	UTSW	12	55,718,114 (GRCm38)	missense	probably benign	0.02
R5114:Ralgapa1	UTSW	12	55,612,723 (GRCm38)	missense	possibly damaging	0.84
R5140:Ralgapa1	UTSW	12	55,665,674 (GRCm38)	missense	probably damaging	1.00
R5140:Ralgapa1	UTSW	12	55,776,152 (GRCm38)	missense	probably damaging	1.00
R5168:Ralgapa1	UTSW	12	55,758,032 (GRCm38)	missense	probably benign	0.05
R5407:Ralgapa1	UTSW	12	55,676,797 (GRCm38)	missense	possibly damaging	0.93
R5441:Ralgapa1	UTSW	12	55,719,623 (GRCm38)	missense	probably damaging	1.00
R5473:Ralgapa1	UTSW	12	55,676,710 (GRCm38)	missense	probably benign	0.41
R5624:Ralgapa1	UTSW	12	55,612,738 (GRCm38)	missense	probably damaging	1.00
R5766:Ralgapa1	UTSW	12	55,820,766 (GRCm38)	start codon destroyed	probably null	0.99
R5826:Ralgapa1	UTSW	12	55,677,113 (GRCm38)	missense	probably damaging	1.00
R5950:Ralgapa1	UTSW	12	55,738,265 (GRCm38)	missense	possibly damaging	0.58
R5980:Ralgapa1	UTSW	12	55,770,616 (GRCm38)	splice site	probably null
R6019:Ralgapa1	UTSW	12	55,684,042 (GRCm38)	missense	possibly damaging	0.92
R6065:Ralgapa1	UTSW	12	55,757,924 (GRCm38)	critical splice donor site	probably null
R6326:Ralgapa1	UTSW	12	55,747,146 (GRCm38)	missense	probably damaging	1.00
R6355:Ralgapa1	UTSW	12	55,698,854 (GRCm38)	missense	probably damaging	1.00
R6408:Ralgapa1	UTSW	12	55,683,910 (GRCm38)	nonsense	probably null
R6448:Ralgapa1	UTSW	12	55,719,661 (GRCm38)	missense	probably benign	0.14
R6453:Ralgapa1	UTSW	12	55,738,319 (GRCm38)	missense	probably damaging	1.00
R6590:Ralgapa1	UTSW	12	55,722,773 (GRCm38)	critical splice donor site	probably null
R6690:Ralgapa1	UTSW	12	55,722,773 (GRCm38)	critical splice donor site	probably null
R6738:Ralgapa1	UTSW	12	55,762,727 (GRCm38)	missense	probably damaging	1.00
R6836:Ralgapa1	UTSW	12	55,604,273 (GRCm38)	splice site	probably null
R6936:Ralgapa1	UTSW	12	55,786,212 (GRCm38)	missense	probably damaging	0.99
R6945:Ralgapa1	UTSW	12	55,776,191 (GRCm38)	missense	possibly damaging	0.64
R7028:Ralgapa1	UTSW	12	55,758,059 (GRCm38)	missense	probably damaging	1.00
R7075:Ralgapa1	UTSW	12	55,820,723 (GRCm38)	missense	possibly damaging	0.66
R7076:Ralgapa1	UTSW	12	55,721,576 (GRCm38)	missense	possibly damaging	0.82
R7098:Ralgapa1	UTSW	12	55,790,310 (GRCm38)	critical splice donor site	probably null
R7231:Ralgapa1	UTSW	12	55,604,191 (GRCm38)	missense	probably damaging	1.00
R7254:Ralgapa1	UTSW	12	55,695,193 (GRCm38)	missense	probably damaging	1.00
R7326:Ralgapa1	UTSW	12	55,709,004 (GRCm38)	missense	probably damaging	1.00
R7485:Ralgapa1	UTSW	12	55,712,672 (GRCm38)	missense	probably damaging	1.00
R7580:Ralgapa1	UTSW	12	55,718,228 (GRCm38)	missense	probably benign	0.00
R7677:Ralgapa1	UTSW	12	55,659,143 (GRCm38)	missense	probably damaging	0.96
R7702:Ralgapa1	UTSW	12	55,709,556 (GRCm38)	missense	probably damaging	1.00
R7702:Ralgapa1	UTSW	12	55,709,555 (GRCm38)	missense	probably damaging	1.00
R7707:Ralgapa1	UTSW	12	55,777,292 (GRCm38)	missense	probably null	0.99
R7723:Ralgapa1	UTSW	12	55,741,513 (GRCm38)	missense	probably benign
R7763:Ralgapa1	UTSW	12	55,757,955 (GRCm38)	missense	probably benign	0.28
R7791:Ralgapa1	UTSW	12	55,741,519 (GRCm38)	missense	probably damaging	0.97
R7812:Ralgapa1	UTSW	12	55,719,628 (GRCm38)	missense	possibly damaging	0.67
R7868:Ralgapa1	UTSW	12	55,612,638 (GRCm38)	missense	probably benign	0.00
R7895:Ralgapa1	UTSW	12	55,747,149 (GRCm38)	missense	probably benign	0.44
R7896:Ralgapa1	UTSW	12	55,697,878 (GRCm38)	missense	probably benign	0.01
R8004:Ralgapa1	UTSW	12	55,702,457 (GRCm38)	missense	probably damaging	0.99
R8094:Ralgapa1	UTSW	12	55,782,846 (GRCm38)	missense	probably damaging	0.99
R8213:Ralgapa1	UTSW	12	55,722,914 (GRCm38)	missense	probably damaging	0.99
R8307:Ralgapa1	UTSW	12	55,741,523 (GRCm38)	missense	probably damaging	0.99
R8423:Ralgapa1	UTSW	12	55,659,062 (GRCm38)	missense	probably damaging	0.99
R8462:Ralgapa1	UTSW	12	55,676,518 (GRCm38)	missense	possibly damaging	0.90
R8469:Ralgapa1	UTSW	12	55,739,413 (GRCm38)	missense	probably damaging	1.00
R8675:Ralgapa1	UTSW	12	55,738,217 (GRCm38)	missense	possibly damaging	0.93
R8802:Ralgapa1	UTSW	12	55,738,316 (GRCm38)	missense	probably damaging	0.99
R8937:Ralgapa1	UTSW	12	55,702,560 (GRCm38)	missense	probably damaging	0.96
R8953:Ralgapa1	UTSW	12	55,820,761 (GRCm38)	missense	probably damaging	0.99
R8974:Ralgapa1	UTSW	12	55,677,006 (GRCm38)	missense	probably benign
R9011:Ralgapa1	UTSW	12	55,605,529 (GRCm38)	intron	probably benign
R9089:Ralgapa1	UTSW	12	55,676,566 (GRCm38)	missense	probably damaging	0.97
R9124:Ralgapa1	UTSW	12	55,735,096 (GRCm38)	missense	probably damaging	1.00
R9254:Ralgapa1	UTSW	12	55,722,798 (GRCm38)	missense	probably damaging	1.00
R9320:Ralgapa1	UTSW	12	55,709,058 (GRCm38)	missense	possibly damaging	0.59
R9379:Ralgapa1	UTSW	12	55,722,798 (GRCm38)	missense	probably damaging	1.00
R9446:Ralgapa1	UTSW	12	55,708,023 (GRCm38)	missense	probably damaging	0.97
R9684:Ralgapa1	UTSW	12	55,612,700 (GRCm38)	missense	possibly damaging	0.63
Z1176:Ralgapa1	UTSW	12	55,709,080 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- cacacacacacacGAGTCTACTTCA -3'
(R):5'- CACAGAACAGCCAGCTCTCAAGTTTAT -3'

Sequencing Primer
(F):5'- tccaaatccttctctctgctc -3'
(R):5'- GCTCTCAAGTTTATGACAATTATGC -3'

Posted On

2013-11-08