Mutagenetix > Incidental Mutation

Incidental Mutation 'R0904:Trbv16'

83246

Institutional Source

Beutler Lab

Gene Symbol

Trbv16

Ensembl Gene

ENSMUSG00000076473

Gene Name

T cell receptor beta, variable 16

Synonyms

Gm16776, Tcrb-V11

MMRRC Submission

039062-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.067) question?

Stock #

R0904 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

41151740-41152230 bp(+) (GRCm38)

Type of Mutation

splice site

DNA Base Change (assembly)

T to C at 41151847 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000100090 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000103274]

AlphaFold

A0A0B4J1H3

Predicted Effect

probably benign
Transcript: ENSMUST00000103274

SMART Domains

Protein: ENSMUSP00000100090
Gene: ENSMUSG00000076473

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
IG_like	37	113	5.7e-7	SMART

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.4%
3x: 98.8%
10x: 97.2%
20x: 94.7%

Validation Efficiency

89% (34/38)

Allele List at MGI

Other mutations in this stock

Total: 35 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700011L22Rik	A	G	8: 79,248,489		probably benign	Het
2810474O19Rik	G	A	6: 149,328,269	A938T	probably damaging	Het
9230110F15Rik	T	C	9: 35,839,070	D102G	probably damaging	Het
Abca13	T	C	11: 9,298,740	V2829A	probably benign	Het
Adk	G	T	14: 21,092,428	D26Y	probably damaging	Het
Bpifb9a	T	C	2: 154,264,225		probably benign	Het
Dap	G	A	15: 31,272,380		probably benign	Het
Eqtn	A	T	4: 94,907,655	S270T	probably benign	Het
Fam193a	A	G	5: 34,462,143	D764G	probably damaging	Het
Fbxl6	A	T	15: 76,537,083		probably null	Het
Gm13212	T	C	4: 145,622,175	Y61H	possibly damaging	Het
Gm3259	A	C	5: 95,341,327	T210P	probably damaging	Het
Gtf3c1	A	T	7: 125,668,842		probably benign	Het
H2-D1	G	C	17: 35,263,861	M122I	probably benign	Het
Lgr6	C	T	1: 134,994,010	A199T	probably damaging	Het
Map1s	C	A	8: 70,914,188	P579Q	probably damaging	Het
Mapk10	A	G	5: 102,987,280		probably benign	Het
Mllt6	C	G	11: 97,664,998	C51W	probably damaging	Het
Mzf1	C	A	7: 13,052,771	R124L	possibly damaging	Het
Naip2	T	C	13: 100,161,854	E558G	probably benign	Het
Naip2	C	T	13: 100,161,860	G556D	probably benign	Het
Neurod2	G	T	11: 98,327,321	T339K	probably benign	Het
Nfya	G	A	17: 48,395,787	Q29*	probably null	Het
Nipbl	A	T	15: 8,361,718	D257E	probably benign	Het
Pex5	G	A	6: 124,399,937		probably benign	Het
Prx	T	A	7: 27,518,294	F879Y	probably damaging	Het
Scai	G	A	2: 39,075,152	T560M	possibly damaging	Het
Slfn10-ps	T	C	11: 83,035,409		noncoding transcript	Het
Spdye4b	A	G	5: 143,195,668		probably benign	Het
Ss18l1	A	G	2: 180,059,354	Y287C	probably damaging	Het
Tpbg	C	A	9: 85,844,564	F195L	unknown	Het
Unc5c	A	G	3: 141,803,840	T620A	probably benign	Het
Vangl1	A	G	3: 102,183,994	S259P	probably damaging	Het
Vmn1r52	T	A	6: 90,179,464	M71K	probably damaging	Het
Vmn2r23	A	T	6: 123,742,135	I816F	probably damaging	Het

Other mutations in Trbv16

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01994:Trbv16	APN	6	41151781	utr 5 prime	probably benign
R4821:Trbv16	UTSW	6	41152002	missense	probably damaging	1.00
R5739:Trbv16	UTSW	6	41152079	missense	probably benign	0.00
R5872:Trbv16	UTSW	6	41152002	missense	probably damaging	1.00
R5965:Trbv16	UTSW	6	41152055	missense	probably benign
R7803:Trbv16	UTSW	6	41151995	missense	not run
R8249:Trbv16	UTSW	6	41151998	missense	possibly damaging	0.47
R8810:Trbv16	UTSW	6	41152038	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TTGGATGAACCACCCATGCCTG -3'
(R):5'- TGTCCTGAAATTGGCTCACACCAC -3'

Sequencing Primer
(F):5'- AAGGTGATCCTTTGAGCAGTGAC -3'
(R):5'- ACACCACAGAGTTGCTTCTTG -3'

Posted On

2013-11-08