|Institutional Source||Beutler Lab|
|Gene Name||phosphoinositide-3-kinase regulatory subunit 5|
|Essential gene?||Probably non essential (E-score: 0.063)|
|Stock #||R0906 (G1)|
|Chromosomal Location||68503019-68552698 bp(+) (GRCm38)|
|Type of Mutation||splice site|
|DNA Base Change (assembly)||T to C at 68536101 bp (GRCm38)|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000099673 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000060441] [ENSMUST00000102613]|
|Meta Mutation Damage Score||0.0898|
|Coding Region Coverage||
|Validation Efficiency||100% (33/33)|
FUNCTION: Phosphoinositide 3-kinase gamma is a lipid kinase that produces the lipid second messenger phosphatidylinositol 3,4,5-trisphosphate. The kinase is composed of a catalytic subunit and one of several regulatory subunits, and is chiefly activated by G protein-coupled receptors. This gene encodes a regulatory subunit, and is distantly related to the phosphoinositide-3-kinase, regulatory subunit 5 gene which is located adjacent to this gene on chromosome 11. The protein binds to both the catalytic subunit and to G beta-gamma, and mediates activation of the kinase subunit downstream of G protein-coupled receptors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit small reductions in lymphocyte and granulocyte and a slight increase in neutrophils. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Pik3r6||
(F):5'- TGCCGTTTAGAGGTCAACAGTGTC -3'
(R):5'- CGTGGGGCTGATTCTAAGGTCAAG -3'
(F):5'- TTAGTGTCAGACAAGTGGCCC -3'
(R):5'- ccctcccccttttcctctc -3'