Mutagenetix > Incidental Mutation

Incidental Mutation 'R0891:Vmn2r75'

83504

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r75

Ensembl Gene

ENSMUSG00000090436

Gene Name

vomeronasal 2, receptor 75

Synonyms

EG546981

MMRRC Submission

039054-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.080) question?

Stock #

R0891 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

85797250-85820932 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 85813476 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glutamic Acid at position 442 (V442E)

Ref Sequence

ENSEMBL: ENSMUSP00000126973 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000167830]

AlphaFold

G5E8Z7

Predicted Effect

possibly damaging
Transcript: ENSMUST00000167830
AA Change: V442E

PolyPhen 2 Score 0.806 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000126973
Gene: ENSMUSG00000090436
AA Change: V442E

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	80	466	2.8e-31	PFAM
Pfam:NCD3G	510	562	4.6e-20	PFAM
Pfam:7tm_3	593	829	7.7e-51	PFAM

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 99.7%
3x: 98.9%
10x: 96.7%
20x: 92.2%

Validation Efficiency

100% (41/41)

Allele List at MGI

Other mutations in this stock

Total: 33 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930522L14Rik	A	T	5: 109,884,156 (GRCm39)	N567K	possibly damaging	Het
Afap1	G	A	5: 36,119,196 (GRCm39)		probably null	Het
Angel2	G	T	1: 190,677,270 (GRCm39)	K517N	possibly damaging	Het
Ankrd36	A	G	11: 5,637,316 (GRCm39)	E1295G	possibly damaging	Het
Ankrd45	A	G	1: 160,982,906 (GRCm39)	N139S	possibly damaging	Het
Ano3	T	C	2: 110,528,321 (GRCm39)	T498A	probably benign	Het
Arhgap12	T	C	18: 6,026,699 (GRCm39)	T720A	probably damaging	Het
Brd10	G	A	19: 29,695,053 (GRCm39)	T1547I	probably damaging	Het
Brsk1	A	G	7: 4,707,226 (GRCm39)	S260G	possibly damaging	Het
Calml3	A	G	13: 3,853,926 (GRCm39)	F93S	probably damaging	Het
Ccnf	G	T	17: 24,445,751 (GRCm39)	H498Q	possibly damaging	Het
Col27a1	A	C	4: 63,223,420 (GRCm39)		probably null	Het
Cpne5	A	G	17: 29,421,893 (GRCm39)		probably benign	Het
Dcst1	G	A	3: 89,260,584 (GRCm39)	T560I	probably benign	Het
Fndc7	A	G	3: 108,777,904 (GRCm39)	Y351H	possibly damaging	Het
Gen1	A	G	12: 11,298,355 (GRCm39)		probably benign	Het
Kcnh8	A	T	17: 53,212,242 (GRCm39)	D680V	probably damaging	Het
Kmt2d	A	G	15: 98,750,572 (GRCm39)		probably benign	Het
Lrrfip1	T	A	1: 90,996,337 (GRCm39)	I50N	probably damaging	Het
Mbip	A	T	12: 56,387,242 (GRCm39)	D132E	possibly damaging	Het
Nipal3	A	T	4: 135,195,898 (GRCm39)	I235N	possibly damaging	Het
Nup93	T	A	8: 95,007,891 (GRCm39)		probably benign	Het
Or6b13	A	G	7: 139,782,372 (GRCm39)	Y104H	probably damaging	Het
Or6z6	T	A	7: 6,491,471 (GRCm39)	Y134F	probably damaging	Het
Pgbd1	T	C	13: 21,606,970 (GRCm39)	Y408C	probably damaging	Het
Pigo	G	A	4: 43,020,519 (GRCm39)	Q808*	probably null	Het
Pik3r1	A	T	13: 101,837,974 (GRCm39)	N299K	probably benign	Het
Pip5k1a	A	G	3: 94,972,831 (GRCm39)		probably benign	Het
Semp2l1	T	A	1: 32,585,442 (GRCm39)	H156L	possibly damaging	Het
Septin5	G	C	16: 18,443,595 (GRCm39)	T118R	probably damaging	Het
Smarcal1	T	C	1: 72,638,015 (GRCm39)	V483A	probably damaging	Het
Togaram1	A	G	12: 65,029,421 (GRCm39)	D948G	probably benign	Het
Zfp57	A	G	17: 37,317,068 (GRCm39)	K46E	probably damaging	Het

Other mutations in Vmn2r75

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01133:Vmn2r75	APN	7	85,797,240 (GRCm39)	unclassified	probably benign
IGL01287:Vmn2r75	APN	7	85,797,801 (GRCm39)	missense	probably damaging	0.97
IGL01318:Vmn2r75	APN	7	85,814,774 (GRCm39)	missense	probably benign	0.06
IGL01331:Vmn2r75	APN	7	85,820,870 (GRCm39)	nonsense	probably null
IGL01406:Vmn2r75	APN	7	85,812,500 (GRCm39)	splice site	probably benign
IGL01615:Vmn2r75	APN	7	85,797,681 (GRCm39)	missense	probably benign	0.03
IGL01657:Vmn2r75	APN	7	85,813,455 (GRCm39)	missense	probably damaging	1.00
IGL02237:Vmn2r75	APN	7	85,814,786 (GRCm39)	missense	possibly damaging	0.88
IGL02275:Vmn2r75	APN	7	85,814,348 (GRCm39)	missense	probably benign	0.04
IGL02307:Vmn2r75	APN	7	85,814,974 (GRCm39)	missense	probably benign	0.00
IGL03136:Vmn2r75	APN	7	85,797,911 (GRCm39)	missense	possibly damaging	0.89
IGL03160:Vmn2r75	APN	7	85,797,644 (GRCm39)	missense	probably damaging	1.00
IGL03244:Vmn2r75	APN	7	85,820,933 (GRCm39)	unclassified	probably benign
PIT4449001:Vmn2r75	UTSW	7	85,814,791 (GRCm39)	missense	probably damaging	1.00
R0049:Vmn2r75	UTSW	7	85,797,309 (GRCm39)	nonsense	probably null
R0049:Vmn2r75	UTSW	7	85,797,309 (GRCm39)	nonsense	probably null
R0083:Vmn2r75	UTSW	7	85,814,866 (GRCm39)	missense	probably benign	0.00
R0108:Vmn2r75	UTSW	7	85,814,866 (GRCm39)	missense	probably benign	0.00
R0276:Vmn2r75	UTSW	7	85,797,515 (GRCm39)	missense	probably benign	0.01
R0320:Vmn2r75	UTSW	7	85,814,288 (GRCm39)	missense	probably benign	0.36
R0471:Vmn2r75	UTSW	7	85,814,721 (GRCm39)	missense	probably benign	0.01
R0562:Vmn2r75	UTSW	7	85,797,449 (GRCm39)	nonsense	probably null
R0631:Vmn2r75	UTSW	7	85,812,478 (GRCm39)	missense	probably null	1.00
R0661:Vmn2r75	UTSW	7	85,814,866 (GRCm39)	missense	probably benign	0.00
R0811:Vmn2r75	UTSW	7	85,814,575 (GRCm39)	missense	probably benign	0.38
R0812:Vmn2r75	UTSW	7	85,814,575 (GRCm39)	missense	probably benign	0.38
R1340:Vmn2r75	UTSW	7	85,797,798 (GRCm39)	missense	probably damaging	0.98
R1501:Vmn2r75	UTSW	7	85,814,850 (GRCm39)	missense	possibly damaging	0.85
R1760:Vmn2r75	UTSW	7	85,798,019 (GRCm39)	missense	probably damaging	1.00
R1970:Vmn2r75	UTSW	7	85,797,470 (GRCm39)	missense	probably damaging	1.00
R2060:Vmn2r75	UTSW	7	85,814,372 (GRCm39)	missense	probably benign	0.00
R2292:Vmn2r75	UTSW	7	85,798,144 (GRCm39)	missense	probably damaging	1.00
R3688:Vmn2r75	UTSW	7	85,797,629 (GRCm39)	missense	probably damaging	0.99
R3892:Vmn2r75	UTSW	7	85,813,494 (GRCm39)	missense	probably null	1.00
R4532:Vmn2r75	UTSW	7	85,797,349 (GRCm39)	nonsense	probably null
R4583:Vmn2r75	UTSW	7	85,813,290 (GRCm39)	missense	possibly damaging	0.81
R4592:Vmn2r75	UTSW	7	85,815,494 (GRCm39)	missense	probably benign	0.00
R4792:Vmn2r75	UTSW	7	85,812,378 (GRCm39)	missense	possibly damaging	0.46
R4859:Vmn2r75	UTSW	7	85,797,611 (GRCm39)	missense	probably benign	0.35
R4896:Vmn2r75	UTSW	7	85,820,787 (GRCm39)	missense	probably benign	0.01
R4943:Vmn2r75	UTSW	7	85,814,705 (GRCm39)	missense	probably damaging	1.00
R4992:Vmn2r75	UTSW	7	85,815,375 (GRCm39)	critical splice donor site	probably null
R5048:Vmn2r75	UTSW	7	85,814,735 (GRCm39)	missense	possibly damaging	0.66
R5063:Vmn2r75	UTSW	7	85,813,372 (GRCm39)	missense	probably benign
R5156:Vmn2r75	UTSW	7	85,813,436 (GRCm39)	missense	possibly damaging	0.51
R5243:Vmn2r75	UTSW	7	85,813,447 (GRCm39)	missense	probably damaging	1.00
R5277:Vmn2r75	UTSW	7	85,815,500 (GRCm39)	missense	probably benign
R5574:Vmn2r75	UTSW	7	85,815,510 (GRCm39)	missense	probably benign	0.22
R5622:Vmn2r75	UTSW	7	85,797,702 (GRCm39)	missense	probably benign	0.15
R5680:Vmn2r75	UTSW	7	85,820,779 (GRCm39)	missense	probably benign	0.10
R5884:Vmn2r75	UTSW	7	85,814,578 (GRCm39)	missense	probably benign
R6021:Vmn2r75	UTSW	7	85,820,820 (GRCm39)	missense	probably benign	0.01
R6217:Vmn2r75	UTSW	7	85,815,375 (GRCm39)	critical splice donor site	probably benign
R6242:Vmn2r75	UTSW	7	85,814,592 (GRCm39)	missense	probably damaging	1.00
R6299:Vmn2r75	UTSW	7	85,814,482 (GRCm39)	missense	probably benign	0.12
R6441:Vmn2r75	UTSW	7	85,820,784 (GRCm39)	missense	probably damaging	0.99
R6495:Vmn2r75	UTSW	7	85,813,287 (GRCm39)	missense	probably benign	0.00
R6553:Vmn2r75	UTSW	7	85,813,453 (GRCm39)	missense	probably benign	0.28
R6670:Vmn2r75	UTSW	7	85,797,644 (GRCm39)	missense	probably damaging	1.00
R7078:Vmn2r75	UTSW	7	85,815,568 (GRCm39)	missense	probably damaging	1.00
R7164:Vmn2r75	UTSW	7	85,814,592 (GRCm39)	missense	probably damaging	1.00
R8411:Vmn2r75	UTSW	7	85,797,722 (GRCm39)	missense	probably damaging	1.00
R8507:Vmn2r75	UTSW	7	85,797,685 (GRCm39)	nonsense	probably null
R8559:Vmn2r75	UTSW	7	85,815,480 (GRCm39)	missense	possibly damaging	0.65
R8677:Vmn2r75	UTSW	7	85,814,410 (GRCm39)	missense	possibly damaging	0.86
R8708:Vmn2r75	UTSW	7	85,812,476 (GRCm39)	missense	probably damaging	0.99
R8778:Vmn2r75	UTSW	7	85,813,497 (GRCm39)	missense	probably benign	0.40
R8968:Vmn2r75	UTSW	7	85,820,765 (GRCm39)	nonsense	probably null
R9145:Vmn2r75	UTSW	7	85,813,447 (GRCm39)	missense	probably damaging	1.00
R9316:Vmn2r75	UTSW	7	85,797,313 (GRCm39)	missense	possibly damaging	0.63
R9363:Vmn2r75	UTSW	7	85,815,423 (GRCm39)	missense	probably benign	0.03

Predicted Primers

PCR Primer
(F):5'- GCTGTTGACCACTTGGAAGATAACCTG -3'
(R):5'- GAGAGCCAACACTGGTATGGAAGTC -3'

Sequencing Primer
(F):5'- CCACTTGGAAGATAACCTGAAAATC -3'
(R):5'- CACTATTGGCAGTTAAAACACAGAG -3'

Posted On

2013-11-08