Mutagenetix > Incidental Mutation

Incidental Mutation 'R0892:Trappc8'

83548

Institutional Source

Beutler Lab

Gene Symbol

Trappc8

Ensembl Gene

ENSMUSG00000033382

Gene Name

trafficking protein particle complex 8

Synonyms

5033403J15Rik, D030074E01Rik, Trs85

MMRRC Submission

039055-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.950) question?

Stock #

R0892 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

20950280-21029150 bp(-) (GRCm39)

Type of Mutation

critical splice donor site (2 bp from exon)

DNA Base Change (assembly)

A to G at 20964665 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000153183 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000025177] [ENSMUST00000225661]

AlphaFold

A0A286YCX6

Predicted Effect

probably null
Transcript: ENSMUST00000025177

SMART Domains

Protein: ENSMUSP00000025177
Gene: ENSMUSG00000033382

Domain	Start	End	E-Value	Type
Pfam:TRAPPC-Trs85	157	604	1e-167	PFAM
low complexity region	769	777	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000225502

Predicted Effect

probably null
Transcript: ENSMUST00000225661

Coding Region Coverage

1x: 99.5%
3x: 98.9%
10x: 97.5%
20x: 95.4%

Validation Efficiency

Allele List at MGI

All alleles(11) : Gene trapped(11)

Other mutations in this stock

Total: 25 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	T	A	11: 9,248,305 (GRCm39)	V2684E	probably benign	Het
Ankub1	T	C	3: 57,597,800 (GRCm39)	R57G	probably benign	Het
Bcr	C	T	10: 74,960,895 (GRCm39)	A442V	probably benign	Het
Cd46	T	C	1: 194,764,920 (GRCm39)	T226A	possibly damaging	Het
Dmkn	C	T	7: 30,466,829 (GRCm39)	R114C	probably damaging	Het
Ell2	A	T	13: 75,911,758 (GRCm39)	N348I	probably damaging	Het
Epg5	T	A	18: 78,011,843 (GRCm39)	I830N	possibly damaging	Het
Gm15737	C	T	6: 92,856,721 (GRCm39)		probably benign	Het
Gpa33	A	G	1: 165,985,211 (GRCm39)	N182S	probably damaging	Het
Gpr37	A	T	6: 25,688,206 (GRCm39)	I297N	probably damaging	Het
Hpse2	T	C	19: 43,376,585 (GRCm39)	K56E	probably benign	Het
Lamc1	G	A	1: 153,208,000 (GRCm39)	H96Y	possibly damaging	Het
Mapre2	A	G	18: 23,991,200 (GRCm39)	N189S	probably benign	Het
Msmo1	T	A	8: 65,175,587 (GRCm39)	I148F	possibly damaging	Het
Myh6	T	C	14: 55,184,511 (GRCm39)	T1607A	probably benign	Het
Oog2	A	T	4: 143,923,069 (GRCm39)	T445S	probably benign	Het
Or5b122	T	A	19: 13,562,881 (GRCm39)	V28D	probably damaging	Het
Plag1	G	A	4: 3,904,532 (GRCm39)	Q220*	probably null	Het
Pom121l2	A	G	13: 22,166,644 (GRCm39)	E305G	possibly damaging	Het
Prorp	A	G	12: 55,429,033 (GRCm39)		probably null	Het
Sbsn	A	T	7: 30,454,244 (GRCm39)	Q50L	possibly damaging	Het
Slc25a42	A	G	8: 70,644,597 (GRCm39)	L34P	probably damaging	Het
Sptbn1	C	A	11: 30,092,201 (GRCm39)	R521S	probably damaging	Het
St14	A	T	9: 31,011,724 (GRCm39)	V394E	probably benign	Het
Tatdn3	T	C	1: 190,795,002 (GRCm39)	D18G	probably benign	Het

Other mutations in Trappc8

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01077:Trappc8	APN	18	20,970,035 (GRCm39)	missense	probably benign	0.20
IGL01367:Trappc8	APN	18	20,999,176 (GRCm39)	missense	probably benign	0.01
IGL01537:Trappc8	APN	18	20,968,061 (GRCm39)	missense	probably benign
IGL01563:Trappc8	APN	18	20,970,103 (GRCm39)	missense	probably benign	0.00
IGL01982:Trappc8	APN	18	21,007,769 (GRCm39)	splice site	probably benign
IGL02709:Trappc8	APN	18	20,970,235 (GRCm39)	missense	possibly damaging	0.94
IGL03126:Trappc8	APN	18	20,996,652 (GRCm39)	missense	probably damaging	1.00
IGL03290:Trappc8	APN	18	20,953,992 (GRCm39)	missense	probably damaging	1.00
IGL03348:Trappc8	APN	18	20,985,838 (GRCm39)	missense	probably damaging	1.00
hoppa	UTSW	18	20,969,957 (GRCm39)	missense	probably benign	0.05
Lagomorpha	UTSW	18	20,951,247 (GRCm39)	missense	probably benign	0.11
rabbit	UTSW	18	21,007,737 (GRCm39)	missense	probably damaging	1.00
E7848:Trappc8	UTSW	18	20,983,975 (GRCm39)	missense	probably damaging	0.99
R0483:Trappc8	UTSW	18	20,978,658 (GRCm39)	missense	possibly damaging	0.60
R0492:Trappc8	UTSW	18	20,999,243 (GRCm39)	missense	probably benign	0.07
R0506:Trappc8	UTSW	18	20,977,245 (GRCm39)	missense	possibly damaging	0.49
R0610:Trappc8	UTSW	18	20,970,245 (GRCm39)	missense	probably damaging	1.00
R1561:Trappc8	UTSW	18	20,974,680 (GRCm39)	nonsense	probably null
R1589:Trappc8	UTSW	18	20,996,608 (GRCm39)	missense	probably damaging	1.00
R1700:Trappc8	UTSW	18	20,966,055 (GRCm39)	missense	probably damaging	1.00
R1785:Trappc8	UTSW	18	20,967,997 (GRCm39)	splice site	probably null
R1786:Trappc8	UTSW	18	20,967,997 (GRCm39)	splice site	probably null
R1989:Trappc8	UTSW	18	20,978,708 (GRCm39)	missense	probably benign	0.04
R2181:Trappc8	UTSW	18	20,952,279 (GRCm39)	critical splice donor site	probably null
R2294:Trappc8	UTSW	18	20,999,211 (GRCm39)	nonsense	probably null
R4551:Trappc8	UTSW	18	21,007,729 (GRCm39)	missense	probably benign	0.10
R4594:Trappc8	UTSW	18	20,970,005 (GRCm39)	missense	probably benign
R4631:Trappc8	UTSW	18	21,000,865 (GRCm39)	missense	probably benign	0.22
R4734:Trappc8	UTSW	18	20,974,629 (GRCm39)	nonsense	probably null
R4834:Trappc8	UTSW	18	20,958,122 (GRCm39)	missense	probably damaging	0.99
R5114:Trappc8	UTSW	18	20,977,237 (GRCm39)	missense	probably benign	0.04
R5262:Trappc8	UTSW	18	20,951,247 (GRCm39)	missense	probably benign	0.11
R5384:Trappc8	UTSW	18	20,966,119 (GRCm39)	splice site	probably null
R5476:Trappc8	UTSW	18	20,998,165 (GRCm39)	missense	probably damaging	1.00
R5503:Trappc8	UTSW	18	20,969,957 (GRCm39)	missense	probably benign	0.05
R5577:Trappc8	UTSW	18	20,969,836 (GRCm39)	nonsense	probably null
R5809:Trappc8	UTSW	18	20,951,139 (GRCm39)	missense	probably benign	0.08
R5825:Trappc8	UTSW	18	21,006,977 (GRCm39)	missense	probably damaging	1.00
R5886:Trappc8	UTSW	18	21,007,737 (GRCm39)	missense	probably damaging	1.00
R5936:Trappc8	UTSW	18	21,007,745 (GRCm39)	missense	probably damaging	1.00
R6024:Trappc8	UTSW	18	20,966,066 (GRCm39)	missense	probably damaging	0.98
R6105:Trappc8	UTSW	18	20,979,504 (GRCm39)	critical splice donor site	probably null
R6229:Trappc8	UTSW	18	21,003,802 (GRCm39)	missense	probably benign	0.00
R6376:Trappc8	UTSW	18	20,970,132 (GRCm39)	missense	probably benign	0.07
R6403:Trappc8	UTSW	18	20,999,128 (GRCm39)	missense	probably benign
R6459:Trappc8	UTSW	18	20,969,925 (GRCm39)	missense	probably benign	0.40
R6673:Trappc8	UTSW	18	21,018,314 (GRCm39)	missense	probably benign	0.01
R7041:Trappc8	UTSW	18	21,007,729 (GRCm39)	missense	probably benign	0.10
R7276:Trappc8	UTSW	18	20,951,148 (GRCm39)	missense	probably damaging	0.99
R7341:Trappc8	UTSW	18	20,985,704 (GRCm39)	missense	probably damaging	1.00
R7684:Trappc8	UTSW	18	20,996,559 (GRCm39)	missense	probably benign	0.01
R7702:Trappc8	UTSW	18	20,958,119 (GRCm39)	missense	probably damaging	0.99
R8210:Trappc8	UTSW	18	21,006,938 (GRCm39)	critical splice donor site	probably null
R8958:Trappc8	UTSW	18	21,003,667 (GRCm39)	missense	probably benign	0.02
R9037:Trappc8	UTSW	18	20,961,539 (GRCm39)	missense	probably benign	0.00
R9217:Trappc8	UTSW	18	21,000,822 (GRCm39)	missense	probably benign	0.01
R9246:Trappc8	UTSW	18	20,993,590 (GRCm39)	missense	possibly damaging	0.64
R9623:Trappc8	UTSW	18	20,983,975 (GRCm39)	missense	possibly damaging	0.91
R9766:Trappc8	UTSW	18	20,979,630 (GRCm39)	missense	possibly damaging	0.68
X0065:Trappc8	UTSW	18	20,993,579 (GRCm39)	missense	probably benign	0.03
Z1177:Trappc8	UTSW	18	20,964,720 (GRCm39)	frame shift	probably null

Predicted Primers

PCR Primer
(F):5'- ACTGTGCATCATTGCATACCTGTTCAT -3'
(R):5'- CCTAGAGCCCCAAGTTCTAACCTTTTG -3'

Sequencing Primer
(F):5'- TGCATACCTGTTCATTTCCAAAG -3'
(R):5'- atccgcctatttctgcctc -3'

Posted On

2013-11-08