Mutagenetix > Incidental Mutation

Incidental Mutation 'IGL01599:Map4'

92024

Institutional Source

Australian Phenomics Network (link to record)

Gene Symbol

Map4

Ensembl Gene

ENSMUSG00000032479

Gene Name

microtubule-associated protein 4

Synonyms

Mtap4, MAP 4

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

IGL01599

Quality Score

Status

Chromosome

Chromosomal Location

109931460-110083955 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 110034768 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Proline to Serine at position 354 (P354S)

Ref Sequence

ENSEMBL: ENSMUSP00000132662 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000035055] [ENSMUST00000165876]

AlphaFold

P27546

Predicted Effect

probably benign
Transcript: ENSMUST00000035055
AA Change: P354S

PolyPhen 2 Score 0.256 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000035055
Gene: ENSMUSG00000032479
AA Change: P354S

Domain	Start	End	E-Value	Type
low complexity region	254	265	N/A	INTRINSIC
internal_repeat_1	266	379	4.96e-7	PROSPERO
low complexity region	401	420	N/A	INTRINSIC
internal_repeat_1	439	550	4.96e-7	PROSPERO
low complexity region	659	674	N/A	INTRINSIC
low complexity region	720	742	N/A	INTRINSIC
low complexity region	760	775	N/A	INTRINSIC
low complexity region	879	889	N/A	INTRINSIC
Pfam:Tubulin-binding	903	926	2e-12	PFAM
Pfam:Tubulin-binding	965	995	4.9e-18	PFAM
Pfam:Tubulin-binding	996	1026	7.4e-18	PFAM
Pfam:Tubulin-binding	1027	1058	4.4e-15	PFAM
low complexity region	1093	1108	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000163190

Predicted Effect

probably benign
Transcript: ENSMUST00000165876
AA Change: P354S

PolyPhen 2 Score 0.256 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000132662
Gene: ENSMUSG00000032479
AA Change: P354S

Domain	Start	End	E-Value	Type
low complexity region	254	265	N/A	INTRINSIC
internal_repeat_1	266	379	4.95e-7	PROSPERO
low complexity region	401	420	N/A	INTRINSIC
internal_repeat_1	439	550	4.95e-7	PROSPERO
low complexity region	659	674	N/A	INTRINSIC
low complexity region	720	742	N/A	INTRINSIC
low complexity region	760	775	N/A	INTRINSIC
low complexity region	879	889	N/A	INTRINSIC
Pfam:Tubulin-binding	896	926	8.5e-16	PFAM
Pfam:Tubulin-binding	965	995	6.4e-19	PFAM
Pfam:Tubulin-binding	996	1026	3.3e-18	PFAM
Pfam:Tubulin-binding	1027	1058	2.3e-11	PFAM
low complexity region	1093	1108	N/A	INTRINSIC

Coding Region Coverage

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a major non-neuronal microtubule-associated protein. This protein contains a domain similar to the microtubule-binding domains of neuronal microtubule-associated protein (MAP2) and microtubule-associated protein tau (MAPT/TAU). This protein promotes microtubule assembly, and has been shown to counteract destabilization of interphase microtubule catastrophe promotion. Cyclin B was found to interact with this protein, which targets cell division cycle 2 (CDC2) kinase to microtubules. The phosphorylation of this protein affects microtubule properties and cell cycle progression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
PHENOTYPE: Mice homozygous for a gene trapped allele are viable and do not display any overt phenotypic abnormalities. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 61 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ackr3	T	C	1: 90,214,134	V105A	probably benign	Het
Acr	T	C	15: 89,568,414	V18A	probably benign	Het
Adgra2	G	A	8: 27,118,733	A540T	possibly damaging	Het
Aldh16a1	A	G	7: 45,142,093	F753L	probably damaging	Het
Ankfy1	T	A	11: 72,738,365	Y338N	probably benign	Het
Aox3	C	T	1: 58,169,794	R829C	probably damaging	Het
Arhgef11	T	C	3: 87,737,046	S1535P	probably benign	Het
C4b	A	G	17: 34,743,019		probably benign	Het
Ccdc157	A	G	11: 4,148,781	C242R	probably damaging	Het
Cep135	T	A	5: 76,593,347	M90K	possibly damaging	Het
Cfap36	A	T	11: 29,244,057		probably null	Het
Chl1	A	G	6: 103,708,484	T829A	probably benign	Het
Copb2	T	C	9: 98,581,150	S473P	probably damaging	Het
Cpb1	C	T	3: 20,251,954		probably null	Het
Cpsf1	A	G	15: 76,596,541	L1295P	probably damaging	Het
Dmp1	C	T	5: 104,212,462	Q335*	probably null	Het
Dyrk1a	T	A	16: 94,691,884	S621T	possibly damaging	Het
Exoc5	T	A	14: 49,034,964	Q331L	probably benign	Het
Fmnl1	G	A	11: 103,186,656	V287M	probably damaging	Het
Fras1	T	C	5: 96,709,891	S2015P	possibly damaging	Het
Gm7052	T	C	17: 22,040,004		probably benign	Het
Gprc5c	A	G	11: 114,864,252	I252V	probably benign	Het
Ints3	C	T	3: 90,394,322		probably null	Het
L1td1	A	G	4: 98,737,344	D592G	probably damaging	Het
Lamb3	A	G	1: 193,343,412	M1137V	probably benign	Het
Leng8	C	A	7: 4,145,482	A751E	probably benign	Het
Lfng	T	C	5: 140,612,535	V204A	probably damaging	Het
Lsm14b	A	G	2: 180,032,603	D233G	probably damaging	Het
Mapt	G	A	11: 104,294,915	V53M	probably damaging	Het
Mier1	T	G	4: 103,155,541	S377A	possibly damaging	Het
Neurog1	T	C	13: 56,251,847	D29G	probably damaging	Het
Npr3	G	A	15: 11,895,789	A257V	probably damaging	Het
Nup188	T	C	2: 30,327,525	V824A	possibly damaging	Het
Olfm4	T	C	14: 80,021,310	S333P	probably damaging	Het
Olfr460	A	C	6: 40,572,252	I289L	probably damaging	Het
Olfr849	T	G	9: 19,441,815	F301V	probably benign	Het
Pbxip1	C	A	3: 89,443,590		probably benign	Het
Pde9a	G	T	17: 31,414,150	C38F	probably damaging	Het
Plb1	A	T	5: 32,342,544		probably benign	Het
Plcz1	T	C	6: 140,002,256		probably benign	Het
Plxnb1	T	C	9: 109,110,604	V1447A	probably damaging	Het
Pnldc1	A	T	17: 12,906,528	M73K	probably benign	Het
Psg20	C	T	7: 18,681,038	V311M	possibly damaging	Het
Psmd2	G	T	16: 20,659,405		probably null	Het
Rabgap1	T	C	2: 37,556,269	V859A	probably damaging	Het
Rad51b	T	A	12: 79,327,228	S194T	probably benign	Het
Rb1cc1	C	T	1: 6,248,771	Q788*	probably null	Het
Ror2	C	T	13: 53,111,617	G468R	probably damaging	Het
Slamf7	A	G	1: 171,641,186	I46T	possibly damaging	Het
Stab2	A	G	10: 86,922,895	S1060P	probably damaging	Het
Syndig1	T	A	2: 150,003,283	V242E	probably damaging	Het
Tgfbr3	G	A	5: 107,118,451	T801M	probably damaging	Het
Tmem132c	T	C	5: 127,359,552		probably benign	Het
Trav21-dv12	T	C	14: 53,876,731	Y103H	probably damaging	Het
Ubr3	T	C	2: 69,938,178	V443A	probably damaging	Het
Uhrf2	G	T	19: 30,092,120	C749F	probably damaging	Het
Ulk2	G	T	11: 61,791,436	S751*	probably null	Het
Wrn	G	A	8: 33,241,011	P1098S	possibly damaging	Het
Xrcc5	T	C	1: 72,346,349	V533A	possibly damaging	Het
Zc3h13	T	C	14: 75,309,723	S223P	probably damaging	Het
Zcchc11	T	C	4: 108,513,399	S871P	possibly damaging	Het

Other mutations in Map4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00565:Map4	APN	9	110072604	splice site	probably benign
IGL01331:Map4	APN	9	110034801	missense	probably benign	0.04
IGL01631:Map4	APN	9	110063133	unclassified	probably benign
IGL02208:Map4	APN	9	109978870	start codon destroyed	probably null	1.00
IGL02455:Map4	APN	9	109999833	missense	probably benign	0.15
IGL02625:Map4	APN	9	110064417	missense	probably damaging	1.00
PIT4486001:Map4	UTSW	9	110072614	missense	probably damaging	1.00
R0149:Map4	UTSW	9	110067624	missense	probably damaging	0.96
R0384:Map4	UTSW	9	110034628	missense	probably damaging	0.99
R0392:Map4	UTSW	9	110078045	missense	probably damaging	1.00
R0496:Map4	UTSW	9	110039850	intron	probably benign
R0526:Map4	UTSW	9	110037278	splice site	probably null
R0555:Map4	UTSW	9	109979103	splice site	probably benign
R0571:Map4	UTSW	9	110036766	missense	probably benign	0.00
R0698:Map4	UTSW	9	110068788	nonsense	probably null
R0762:Map4	UTSW	9	110038478	intron	probably benign
R0862:Map4	UTSW	9	109978969	missense	probably damaging	1.00
R0864:Map4	UTSW	9	109978969	missense	probably damaging	1.00
R1168:Map4	UTSW	9	110034964	missense	probably benign	0.00
R1238:Map4	UTSW	9	110068580	missense	probably benign	0.00
R1735:Map4	UTSW	9	110034955	missense	probably benign	0.00
R1869:Map4	UTSW	9	110034964	missense	probably benign	0.00
R1869:Map4	UTSW	9	110068928	missense	possibly damaging	0.95
R2196:Map4	UTSW	9	110071048	missense	probably damaging	1.00
R2264:Map4	UTSW	9	110081457	missense	probably damaging	1.00
R2507:Map4	UTSW	9	110037483	intron	probably benign
R2512:Map4	UTSW	9	110034702	missense	possibly damaging	0.48
R3087:Map4	UTSW	9	110053189	missense	possibly damaging	0.84
R3154:Map4	UTSW	9	109999792	missense	probably benign	0.19
R3498:Map4	UTSW	9	110035212	missense	probably benign	0.03
R3547:Map4	UTSW	9	110052198	missense	possibly damaging	0.61
R3751:Map4	UTSW	9	110038674	intron	probably benign
R4036:Map4	UTSW	9	110032215	missense	possibly damaging	0.47
R4423:Map4	UTSW	9	110067594	missense	probably damaging	1.00
R4505:Map4	UTSW	9	110032185	missense	probably benign	0.01
R4561:Map4	UTSW	9	110052371	missense	possibly damaging	0.91
R4577:Map4	UTSW	9	110081421	missense	possibly damaging	0.48
R4601:Map4	UTSW	9	110052819	missense	possibly damaging	0.75
R4795:Map4	UTSW	9	110035263	missense	probably benign	0.00
R4801:Map4	UTSW	9	110035257	missense	probably benign	0.15
R4802:Map4	UTSW	9	110035257	missense	probably benign	0.15
R4999:Map4	UTSW	9	110038377	intron	probably benign
R5020:Map4	UTSW	9	110068800	missense	probably benign	0.02
R5021:Map4	UTSW	9	110038089	nonsense	probably null
R5049:Map4	UTSW	9	110079814	nonsense	probably null
R5451:Map4	UTSW	9	110037783	intron	probably benign
R5452:Map4	UTSW	9	110037783	intron	probably benign
R5453:Map4	UTSW	9	110037783	intron	probably benign
R5492:Map4	UTSW	9	110052382	missense	possibly damaging	0.68
R5532:Map4	UTSW	9	110034678	missense	probably benign	0.24
R5602:Map4	UTSW	9	110052700	missense	possibly damaging	0.84
R5628:Map4	UTSW	9	110081847	missense	probably benign	0.04
R5896:Map4	UTSW	9	110072634	missense	possibly damaging	0.91
R6017:Map4	UTSW	9	110034619	missense	probably benign	0.00
R6084:Map4	UTSW	9	110064292	missense	probably damaging	1.00
R6294:Map4	UTSW	9	110002746	missense	possibly damaging	0.82
R6397:Map4	UTSW	9	110027716	missense	possibly damaging	0.78
R6773:Map4	UTSW	9	110034925	missense	probably benign	0.00
R6997:Map4	UTSW	9	110052914	missense	probably benign	0.35
R7141:Map4	UTSW	9	109978870	start codon destroyed	probably null	1.00
R7187:Map4	UTSW	9	110053133	missense	probably benign	0.03
R7320:Map4	UTSW	9	110081517	missense	probably benign	0.24
R7469:Map4	UTSW	9	110027797	splice site	probably null
R7479:Map4	UTSW	9	110068824	missense	possibly damaging	0.94
R7487:Map4	UTSW	9	110027715	missense	probably damaging	1.00
R7690:Map4	UTSW	9	109999793	missense	probably damaging	0.99
R7780:Map4	UTSW	9	110034652	missense	probably benign	0.00
R7998:Map4	UTSW	9	110079861	missense	probably damaging	1.00
R8028:Map4	UTSW	9	110068744	missense	probably damaging	1.00
R8557:Map4	UTSW	9	110064302	splice site	probably null
R8950:Map4	UTSW	9	110072634	missense	possibly damaging	0.91
R8972:Map4	UTSW	9	110035117	missense	probably benign
R9145:Map4	UTSW	9	110026200	missense	probably damaging	0.99
R9297:Map4	UTSW	9	110053412	missense	probably benign	0.02
R9332:Map4	UTSW	9	110035155	missense	probably benign	0.00
R9354:Map4	UTSW	9	110068779	missense	probably benign
R9419:Map4	UTSW	9	110052961	missense	possibly damaging	0.92
R9430:Map4	UTSW	9	110034692	missense	probably benign	0.41
R9437:Map4	UTSW	9	110035087	missense	possibly damaging	0.46
R9718:Map4	UTSW	9	110072706	critical splice donor site	probably null
Z1177:Map4	UTSW	9	110068523	critical splice acceptor site	probably null

Posted On

2013-12-09