Mutagenetix > Incidental Mutation

Incidental Mutation 'IGL01642:Prmt2'

93709

Institutional Source

Australian Phenomics Network (link to record)

Gene Symbol

Prmt2

Ensembl Gene

ENSMUSG00000020230

Gene Name

protein arginine N-methyltransferase 2

Synonyms

Hrmt1l1

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

IGL01642

Quality Score

Status

Chromosome

Chromosomal Location

76043060-76073699 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 76058327 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Serine at position 161 (G161S)

Ref Sequence

ENSEMBL: ENSMUSP00000097167 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020452] [ENSMUST00000099571] [ENSMUST00000099572] [ENSMUST00000128099] [ENSMUST00000137857] [ENSMUST00000217726]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000020452
AA Change: G161S

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000020452
Gene: ENSMUSG00000020230
AA Change: G161S

Domain	Start	End	E-Value	Type
low complexity region	30	43	N/A	INTRINSIC
SH3	45	100	4.22e-15	SMART
Pfam:PrmA	122	253	2.5e-8	PFAM
Pfam:PRMT5	123	427	2.4e-13	PFAM
Pfam:Met_10	127	244	5.1e-8	PFAM
Pfam:MTS	134	223	5.7e-11	PFAM
Pfam:Methyltransf_31	147	294	1.5e-8	PFAM
Pfam:Methyltransf_26	150	224	1.2e-9	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000099571
AA Change: G161S

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000097166
Gene: ENSMUSG00000020230
AA Change: G161S

Domain	Start	End	E-Value	Type
low complexity region	30	43	N/A	INTRINSIC
SH3	45	100	4.22e-15	SMART
Pfam:PrmA	122	253	2.5e-8	PFAM
Pfam:PRMT5	123	427	2.4e-13	PFAM
Pfam:Met_10	127	244	5.1e-8	PFAM
Pfam:MTS	134	223	5.7e-11	PFAM
Pfam:Methyltransf_31	147	294	1.5e-8	PFAM
Pfam:Methyltransf_26	150	224	1.2e-9	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000099572
AA Change: G161S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000097167
Gene: ENSMUSG00000020230
AA Change: G161S

Domain	Start	End	E-Value	Type
low complexity region	30	43	N/A	INTRINSIC
SH3	45	100	4.22e-15	SMART
Pfam:PrmA	124	253	3.1e-8	PFAM
Pfam:PRMT5	124	451	1.2e-11	PFAM
Pfam:MTS	137	223	3.3e-10	PFAM
Pfam:Methyltransf_31	147	294	1.7e-8	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000128099
AA Change: G161S

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000137707
Gene: ENSMUSG00000020230
AA Change: G161S

Domain	Start	End	E-Value	Type
low complexity region	30	43	N/A	INTRINSIC
SH3	45	100	4.22e-15	SMART
Pfam:PrmA	120	253	1.3e-9	PFAM
Pfam:Met_10	122	235	3.8e-8	PFAM
Pfam:TehB	122	235	6.9e-8	PFAM
Pfam:MTS	133	223	2e-11	PFAM
Pfam:Methyltransf_31	147	243	9.3e-9	PFAM
Pfam:Methyltransf_26	150	224	4.6e-10	PFAM
Pfam:Methyltransf_11	154	238	3.2e-6	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000137857
AA Change: G161S

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000137725
Gene: ENSMUSG00000020230
AA Change: G161S

Domain	Start	End	E-Value	Type
low complexity region	30	43	N/A	INTRINSIC
SH3	45	100	4.22e-15	SMART
Pfam:PrmA	120	253	1.5e-9	PFAM
Pfam:Met_10	129	235	4.2e-7	PFAM
Pfam:MTS	137	223	1.1e-10	PFAM
Pfam:Methyltransf_31	147	243	9.2e-9	PFAM
Pfam:Methyltransf_11	154	237	1.7e-6	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000217726

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000220116

Coding Region Coverage

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele display a hyperplastic response to vascular injury while mutant mouse embryonic fibroblasts show an earlier S phase entry following release of serum starvation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aanat	A	G	11: 116,486,514 (GRCm39)	T18A	possibly damaging	Het
Abat	A	T	16: 8,418,783 (GRCm39)	I126F	possibly damaging	Het
Adcy6	G	A	15: 98,492,390 (GRCm39)	A958V	possibly damaging	Het
Ago2	T	C	15: 72,995,239 (GRCm39)	I447V	probably benign	Het
Arid1a	A	G	4: 133,409,155 (GRCm39)	V1784A	unknown	Het
Atp1b1	A	G	1: 164,285,330 (GRCm39)	F33L	probably benign	Het
Bpnt1	G	A	1: 185,086,238 (GRCm39)	V198I	probably benign	Het
Cemip2	T	A	19: 21,801,265 (GRCm39)	I794N	probably damaging	Het
Cfap251	T	C	5: 123,426,761 (GRCm39)	V383A	possibly damaging	Het
Cgas	G	A	9: 78,344,680 (GRCm39)	P247L	probably damaging	Het
Clip3	G	A	7: 29,998,287 (GRCm39)	M244I	probably benign	Het
Clip3	A	T	7: 29,996,494 (GRCm39)		probably benign	Het
Cyp2a22	A	T	7: 26,638,184 (GRCm39)	N107K	possibly damaging	Het
Cyp2c23	A	T	19: 43,993,995 (GRCm39)	L457Q	probably damaging	Het
Dbr1	A	G	9: 99,458,031 (GRCm39)	Y17C	probably damaging	Het
Drc7	G	A	8: 95,785,767 (GRCm39)	V208I	probably benign	Het
Dst	T	A	1: 34,228,470 (GRCm39)	L2021Q	probably damaging	Het
E2f4	C	A	8: 106,027,968 (GRCm39)	P299T	probably damaging	Het
Eef1b2	T	C	1: 63,216,990 (GRCm39)	L53P	probably damaging	Het
Enpp3	T	C	10: 24,674,167 (GRCm39)	T378A	probably damaging	Het
Eps15	A	G	4: 109,223,670 (GRCm39)	N302S	probably benign	Het
Esrrg	G	A	1: 187,943,112 (GRCm39)	V362M	probably benign	Het
Gcc2	T	A	10: 58,116,434 (GRCm39)	N1014K	probably benign	Het
Gm3099	T	A	14: 15,346,476 (GRCm39)	M114K	possibly damaging	Het
Gnptab	C	T	10: 88,271,994 (GRCm39)	T928I	possibly damaging	Het
Gpd2	A	T	2: 57,158,083 (GRCm39)	R31*	probably null	Het
Impdh1	T	C	6: 29,207,165 (GRCm39)	T60A	possibly damaging	Het
Kcnab3	A	G	11: 69,221,256 (GRCm39)	E191G	probably benign	Het
Kcnh5	A	G	12: 75,011,943 (GRCm39)	S659P	probably damaging	Het
Kl	T	C	5: 150,904,334 (GRCm39)	I362T	possibly damaging	Het
Kpna4	A	G	3: 68,993,117 (GRCm39)	V414A	probably damaging	Het
Magi1	G	A	6: 93,663,605 (GRCm39)	P1111S	possibly damaging	Het
Myo18a	A	G	11: 77,755,558 (GRCm39)	D1965G	probably benign	Het
Nadsyn1	G	A	7: 143,351,615 (GRCm39)	P673S	probably damaging	Het
Naip2	A	G	13: 100,297,445 (GRCm39)	S864P	probably damaging	Het
Or10ak16	A	G	4: 118,750,658 (GRCm39)	Y126C	probably damaging	Het
Or2d2b	A	T	7: 106,706,029 (GRCm39)	I13N	possibly damaging	Het
Or2f1	G	T	6: 42,721,486 (GRCm39)	V172L	probably benign	Het
Paics	T	A	5: 77,109,357 (GRCm39)		probably benign	Het
Papss1	G	T	3: 131,288,996 (GRCm39)		probably benign	Het
Pax3	A	G	1: 78,173,300 (GRCm39)		probably null	Het
Pgbd5	T	G	8: 125,110,941 (GRCm39)	Q159P	probably benign	Het
Pkd1	A	G	17: 24,800,266 (GRCm39)	Y3009C	probably damaging	Het
Pla2g4d	T	C	2: 120,111,117 (GRCm39)	T161A	probably damaging	Het
Podxl2	T	A	6: 88,820,529 (GRCm39)	Y521F	probably damaging	Het
Pramel34	A	G	5: 93,784,154 (GRCm39)	Y437H	possibly damaging	Het
Rft1	C	T	14: 30,398,825 (GRCm39)	T265I	probably damaging	Het
Rims2	T	A	15: 39,321,192 (GRCm39)	L736M	probably damaging	Het
Slf1	C	T	13: 77,198,034 (GRCm39)	A747T	probably benign	Het
Snx2	A	G	18: 53,349,519 (GRCm39)	K427E	probably damaging	Het
Tmem131l	A	T	3: 83,845,357 (GRCm39)	D424E	possibly damaging	Het
Tnxb	A	G	17: 34,937,488 (GRCm39)	T3826A	probably damaging	Het
Tpp2	T	C	1: 43,993,813 (GRCm39)	Y233H	probably damaging	Het
Ubl4b	C	A	3: 107,462,147 (GRCm39)	E38*	probably null	Het
Usp5	A	T	6: 124,797,416 (GRCm39)	I486N	probably damaging	Het
Vmn1r193	A	C	13: 22,403,794 (GRCm39)	L66R	probably damaging	Het
Vps13b	T	C	15: 35,792,218 (GRCm39)	I2162T	probably benign	Het
Wipf2	T	C	11: 98,781,650 (GRCm39)	V63A	probably benign	Het
Zfp735	G	T	11: 73,601,305 (GRCm39)	C83F	possibly damaging	Het
Zfyve26	T	C	12: 79,308,348 (GRCm39)		probably null	Het

Other mutations in Prmt2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01663:Prmt2	APN	10	76,053,143 (GRCm39)	splice site	probably null
IGL02015:Prmt2	APN	10	76,062,089 (GRCm39)	nonsense	probably null
IGL03094:Prmt2	APN	10	76,046,224 (GRCm39)	splice site	probably benign
R0352:Prmt2	UTSW	10	76,044,337 (GRCm39)	missense	possibly damaging	0.89
R0617:Prmt2	UTSW	10	76,044,517 (GRCm39)	intron	probably benign
R0831:Prmt2	UTSW	10	76,043,641 (GRCm39)	unclassified	probably benign
R0885:Prmt2	UTSW	10	76,058,399 (GRCm39)	missense	probably damaging	1.00
R1882:Prmt2	UTSW	10	76,058,302 (GRCm39)	missense	probably benign	0.00
R2022:Prmt2	UTSW	10	76,061,292 (GRCm39)	nonsense	probably null
R2312:Prmt2	UTSW	10	76,062,089 (GRCm39)	nonsense	probably null
R2401:Prmt2	UTSW	10	76,061,249 (GRCm39)	nonsense	probably null
R2408:Prmt2	UTSW	10	76,044,301 (GRCm39)	missense	probably damaging	0.98
R3753:Prmt2	UTSW	10	76,061,137 (GRCm39)	missense	probably benign	0.01
R4707:Prmt2	UTSW	10	76,062,055 (GRCm39)	missense	probably damaging	0.96
R4785:Prmt2	UTSW	10	76,062,055 (GRCm39)	missense	probably damaging	0.96
R4937:Prmt2	UTSW	10	76,056,842 (GRCm39)	missense	probably damaging	1.00
R5072:Prmt2	UTSW	10	76,058,390 (GRCm39)	missense	probably damaging	1.00
R5073:Prmt2	UTSW	10	76,058,390 (GRCm39)	missense	probably damaging	1.00
R5074:Prmt2	UTSW	10	76,058,390 (GRCm39)	missense	probably damaging	1.00
R5851:Prmt2	UTSW	10	76,072,574 (GRCm39)	missense	possibly damaging	0.61
R6084:Prmt2	UTSW	10	76,046,278 (GRCm39)	missense	probably benign	0.23
R6120:Prmt2	UTSW	10	76,045,280 (GRCm39)	missense	possibly damaging	0.51
R6239:Prmt2	UTSW	10	76,058,425 (GRCm39)	nonsense	probably null
R6317:Prmt2	UTSW	10	76,058,351 (GRCm39)	missense	probably benign	0.15
R6659:Prmt2	UTSW	10	76,053,208 (GRCm39)	missense	possibly damaging	0.85
R7174:Prmt2	UTSW	10	76,061,173 (GRCm39)	missense	probably benign	0.00
R7421:Prmt2	UTSW	10	76,056,912 (GRCm39)	missense	probably benign	0.00
R7485:Prmt2	UTSW	10	76,056,838 (GRCm39)	nonsense	probably null
R8326:Prmt2	UTSW	10	76,053,247 (GRCm39)	missense	probably benign	0.00
R9490:Prmt2	UTSW	10	76,053,227 (GRCm39)	missense	probably damaging	0.98
R9620:Prmt2	UTSW	10	76,061,213 (GRCm39)	missense	probably damaging	1.00
R9694:Prmt2	UTSW	10	76,061,213 (GRCm39)	missense	probably damaging	1.00

Posted On

2013-12-09