Incidental Mutation 'R1034:Zfp352'
| ID |
95383 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp352
|
| Ensembl Gene |
ENSMUSG00000070902 |
| Gene Name |
zinc finger protein 352 |
| Synonyms |
2czf48 |
| MMRRC Submission |
039133-MU
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R1034 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
4 |
| Chromosomal Location |
90218820-90225702 bp(+) (GRCm38) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to A
at 90224156 bp (GRCm38)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Serine to Threonine
at position 178
(S178T)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000102746
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000080541]
[ENSMUST00000107129]
|
| AlphaFold |
A2AML7 |
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000080541
AA Change: S178T
PolyPhen 2
Score 0.944 (Sensitivity: 0.80; Specificity: 0.95)
|
| SMART Domains |
Protein: ENSMUSP00000079383
Gene: ENSMUSG00000070902
AA Change: S178T
| Domain | Start | End | E-Value | Type |
|---|
|
ZnF_C2H2
|
459 |
483 |
3.34e-2 |
SMART |
|
ZnF_C2H2
|
489 |
513 |
8.22e-2 |
SMART |
|
ZnF_C2H2
|
519 |
542 |
1.76e-1 |
SMART |
|
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000107129
AA Change: S178T
PolyPhen 2
Score 0.944 (Sensitivity: 0.80; Specificity: 0.95)
|
| SMART Domains |
Protein: ENSMUSP00000102746
Gene: ENSMUSG00000070902
AA Change: S178T
| Domain | Start | End | E-Value | Type |
|---|
|
ZnF_C2H2
|
459 |
483 |
3.34e-2 |
SMART |
|
ZnF_C2H2
|
489 |
513 |
8.22e-2 |
SMART |
|
ZnF_C2H2
|
519 |
542 |
1.76e-1 |
SMART |
|
| Coding Region Coverage |
- 1x: 98.7%
- 3x: 97.3%
- 10x: 92.9%
- 20x: 82.2%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 34 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Aasdh |
G |
A |
5: 76,876,283 (GRCm38) |
T174M |
probably damaging |
Het |
| Abca14 |
T |
C |
7: 120,216,147 (GRCm38) |
F206S |
probably damaging |
Het |
| Arid2 |
T |
C |
15: 96,369,505 (GRCm38) |
V622A |
probably benign |
Het |
| Asic1 |
T |
A |
15: 99,698,058 (GRCm38) |
L437Q |
probably damaging |
Het |
| Atp1b1 |
C |
T |
1: 164,453,488 (GRCm38) |
|
probably null |
Het |
| B3gnt5 |
A |
G |
16: 19,769,484 (GRCm38) |
Y151C |
probably damaging |
Het |
| Cbx4 |
A |
G |
11: 119,081,707 (GRCm38) |
S281P |
probably damaging |
Het |
| Dnah5 |
G |
A |
15: 28,302,471 (GRCm38) |
V1625I |
probably damaging |
Het |
| Eftud2 |
A |
C |
11: 102,849,184 (GRCm38) |
D461E |
probably benign |
Het |
| Epgn |
A |
G |
5: 91,032,221 (GRCm38) |
Y74C |
probably damaging |
Het |
| Fcho1 |
C |
T |
8: 71,712,560 (GRCm38) |
A418T |
probably benign |
Het |
| Fgf14 |
C |
T |
14: 124,132,534 (GRCm38) |
V113I |
probably damaging |
Het |
| Ggcx |
G |
A |
6: 72,414,831 (GRCm38) |
R68H |
probably damaging |
Het |
| Gm1110 |
A |
G |
9: 26,921,350 (GRCm38) |
S16P |
probably damaging |
Het |
| Gm6729 |
T |
C |
10: 86,540,026 (GRCm38) |
|
noncoding transcript |
Het |
| Gpr108 |
A |
G |
17: 57,235,995 (GRCm38) |
F522S |
probably damaging |
Het |
| Gpr156 |
T |
C |
16: 38,004,726 (GRCm38) |
V435A |
probably benign |
Het |
| Khdrbs2 |
T |
C |
1: 32,467,791 (GRCm38) |
L172P |
probably damaging |
Het |
| Kmo |
C |
T |
1: 175,651,618 (GRCm38) |
P240L |
possibly damaging |
Het |
| Ltn1 |
A |
T |
16: 87,397,137 (GRCm38) |
|
probably null |
Het |
| Nbeal1 |
T |
A |
1: 60,290,006 (GRCm38) |
Y2194* |
probably null |
Het |
| Olfr767 |
T |
A |
10: 129,079,961 (GRCm38) |
M1L |
probably benign |
Het |
| Olfr867 |
A |
T |
9: 20,055,365 (GRCm38) |
S33T |
probably benign |
Het |
| Rab6b |
T |
C |
9: 103,167,124 (GRCm38) |
S172P |
probably benign |
Het |
| Ror1 |
T |
A |
4: 100,333,620 (GRCm38) |
L58* |
probably null |
Het |
| Sec23b |
A |
G |
2: 144,590,338 (GRCm38) |
D756G |
possibly damaging |
Het |
| Slc24a2 |
T |
A |
4: 87,032,275 (GRCm38) |
K428N |
probably damaging |
Het |
| Spen |
C |
A |
4: 141,475,752 (GRCm38) |
V1855L |
probably benign |
Het |
| Srgap1 |
G |
A |
10: 121,785,445 (GRCm38) |
P1071S |
possibly damaging |
Het |
| Tns1 |
A |
G |
1: 73,941,969 (GRCm38) |
C1079R |
probably damaging |
Het |
| Traf3ip1 |
G |
T |
1: 91,518,319 (GRCm38) |
|
probably null |
Het |
| Trmt2a |
T |
C |
16: 18,249,709 (GRCm38) |
F82S |
probably damaging |
Het |
| Xrn1 |
A |
G |
9: 96,039,737 (GRCm38) |
D1401G |
probably damaging |
Het |
| Zfp758 |
G |
T |
17: 22,375,759 (GRCm38) |
E409* |
probably null |
Het |
|
| Other mutations in Zfp352 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01963:Zfp352
|
APN |
4 |
90,224,154 (GRCm38) |
missense |
possibly damaging |
0.95 |
|
IGL02252:Zfp352
|
APN |
4 |
90,224,130 (GRCm38) |
missense |
probably benign |
0.02 |
|
IGL03156:Zfp352
|
APN |
4 |
90,224,087 (GRCm38) |
missense |
possibly damaging |
0.57 |
|
IGL03167:Zfp352
|
APN |
4 |
90,224,702 (GRCm38) |
missense |
probably damaging |
0.99 |
|
IGL03190:Zfp352
|
APN |
4 |
90,223,757 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
IGL03335:Zfp352
|
APN |
4 |
90,224,346 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R0051:Zfp352
|
UTSW |
4 |
90,224,285 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R0403:Zfp352
|
UTSW |
4 |
90,225,009 (GRCm38) |
missense |
possibly damaging |
0.60 |
|
R0550:Zfp352
|
UTSW |
4 |
90,224,690 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R0671:Zfp352
|
UTSW |
4 |
90,223,919 (GRCm38) |
missense |
probably benign |
|
|
R1754:Zfp352
|
UTSW |
4 |
90,223,809 (GRCm38) |
missense |
probably benign |
0.23 |
|
R2016:Zfp352
|
UTSW |
4 |
90,225,171 (GRCm38) |
missense |
probably benign |
0.42 |
|
R2064:Zfp352
|
UTSW |
4 |
90,225,120 (GRCm38) |
missense |
probably benign |
0.08 |
|
R2308:Zfp352
|
UTSW |
4 |
90,225,243 (GRCm38) |
missense |
probably benign |
0.00 |
|
R3552:Zfp352
|
UTSW |
4 |
90,225,102 (GRCm38) |
missense |
probably benign |
0.33 |
|
R3794:Zfp352
|
UTSW |
4 |
90,225,149 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R3795:Zfp352
|
UTSW |
4 |
90,225,149 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R4135:Zfp352
|
UTSW |
4 |
90,225,024 (GRCm38) |
missense |
probably damaging |
0.96 |
|
R4356:Zfp352
|
UTSW |
4 |
90,223,834 (GRCm38) |
missense |
possibly damaging |
0.91 |
|
R4409:Zfp352
|
UTSW |
4 |
90,225,164 (GRCm38) |
missense |
probably benign |
0.00 |
|
R4590:Zfp352
|
UTSW |
4 |
90,224,535 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R4614:Zfp352
|
UTSW |
4 |
90,225,081 (GRCm38) |
missense |
probably benign |
0.00 |
|
R4617:Zfp352
|
UTSW |
4 |
90,225,081 (GRCm38) |
missense |
probably benign |
0.00 |
|
R4618:Zfp352
|
UTSW |
4 |
90,225,081 (GRCm38) |
missense |
probably benign |
0.00 |
|
R4741:Zfp352
|
UTSW |
4 |
90,224,940 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R4931:Zfp352
|
UTSW |
4 |
90,224,304 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R4959:Zfp352
|
UTSW |
4 |
90,224,139 (GRCm38) |
missense |
probably benign |
0.01 |
|
R4973:Zfp352
|
UTSW |
4 |
90,224,139 (GRCm38) |
missense |
probably benign |
0.01 |
|
R5167:Zfp352
|
UTSW |
4 |
90,224,216 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R5260:Zfp352
|
UTSW |
4 |
90,224,460 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R5524:Zfp352
|
UTSW |
4 |
90,225,104 (GRCm38) |
missense |
possibly damaging |
0.95 |
|
R5942:Zfp352
|
UTSW |
4 |
90,225,070 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R6802:Zfp352
|
UTSW |
4 |
90,225,200 (GRCm38) |
missense |
probably benign |
0.33 |
|
R6819:Zfp352
|
UTSW |
4 |
90,224,699 (GRCm38) |
missense |
probably benign |
|
|
R7072:Zfp352
|
UTSW |
4 |
90,224,424 (GRCm38) |
missense |
probably benign |
0.00 |
|
R7099:Zfp352
|
UTSW |
4 |
90,224,880 (GRCm38) |
missense |
probably benign |
0.00 |
|
R7569:Zfp352
|
UTSW |
4 |
90,223,659 (GRCm38) |
missense |
possibly damaging |
0.77 |
|
R7645:Zfp352
|
UTSW |
4 |
90,224,777 (GRCm38) |
missense |
probably benign |
0.13 |
|
R7705:Zfp352
|
UTSW |
4 |
90,225,275 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R8424:Zfp352
|
UTSW |
4 |
90,224,243 (GRCm38) |
missense |
possibly damaging |
0.87 |
|
R9180:Zfp352
|
UTSW |
4 |
90,224,881 (GRCm38) |
missense |
probably benign |
0.38 |
|
R9378:Zfp352
|
UTSW |
4 |
90,224,338 (GRCm38) |
missense |
probably benign |
0.13 |
|
R9509:Zfp352
|
UTSW |
4 |
90,224,706 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R9623:Zfp352
|
UTSW |
4 |
90,224,891 (GRCm38) |
missense |
probably benign |
0.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- TGGAACCAGCAAGTTGTGTCTGC -3'
(R):5'- TGATAACCAGAGAGGGAGCACTCAC -3'
Sequencing Primer
(F):5'- GTTGACACCAGGGAGACTTTCTAC -3'
(R):5'- GCTCTCATCTGAGGCTCATAGAAAG -3'
|
| Posted On |
2014-01-05 |
Incidental Mutation