Mutagenetix > Incidental Mutation

Incidental Mutation 'R1034:Gpr108'

95488

Institutional Source

Beutler Lab

Gene Symbol

Gpr108

Ensembl Gene

ENSMUSG00000005823

Gene Name

G protein-coupled receptor 108

Synonyms

1810015L19Rik

MMRRC Submission

039133-MU

Accession Numbers

Genbank: NM_030084.3; Ensembl: ENSMUST00000005975

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1034 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

57234914-57247641 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 57235995 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Serine at position 522 (F522S)

Ref Sequence

ENSEMBL: ENSMUSP00000005975 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000005975]

AlphaFold

Q91WD0

Predicted Effect

probably damaging
Transcript: ENSMUST00000005975
AA Change: F522S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000005975
Gene: ENSMUSG00000005823
AA Change: F522S

Domain	Start	End	E-Value	Type
low complexity region	61	75	N/A	INTRINSIC
low complexity region	86	103	N/A	INTRINSIC
Pfam:Lung_7-TM_R	240	529	2.3e-98	PFAM
low complexity region	535	547	N/A	INTRINSIC

Coding Region Coverage

1x: 98.7%
3x: 97.3%
10x: 92.9%
20x: 82.2%

Validation Efficiency

Allele List at MGI

All alleles(3) : Targeted, other(2) Gene trapped(1)

Other mutations in this stock

Total: 34 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aasdh	G	A	5: 76,876,283	T174M	probably damaging	Het
Abca14	T	C	7: 120,216,147	F206S	probably damaging	Het
Arid2	T	C	15: 96,369,505	V622A	probably benign	Het
Asic1	T	A	15: 99,698,058	L437Q	probably damaging	Het
Atp1b1	C	T	1: 164,453,488		probably null	Het
B3gnt5	A	G	16: 19,769,484	Y151C	probably damaging	Het
Cbx4	A	G	11: 119,081,707	S281P	probably damaging	Het
Dnah5	G	A	15: 28,302,471	V1625I	probably damaging	Het
Eftud2	A	C	11: 102,849,184	D461E	probably benign	Het
Epgn	A	G	5: 91,032,221	Y74C	probably damaging	Het
Fcho1	C	T	8: 71,712,560	A418T	probably benign	Het
Fgf14	C	T	14: 124,132,534	V113I	probably damaging	Het
Ggcx	G	A	6: 72,414,831	R68H	probably damaging	Het
Gm1110	A	G	9: 26,921,350	S16P	probably damaging	Het
Gm6729	T	C	10: 86,540,026		noncoding transcript	Het
Gpr156	T	C	16: 38,004,726	V435A	probably benign	Het
Khdrbs2	T	C	1: 32,467,791	L172P	probably damaging	Het
Kmo	C	T	1: 175,651,618	P240L	possibly damaging	Het
Ltn1	A	T	16: 87,397,137		probably null	Het
Nbeal1	T	A	1: 60,290,006	Y2194*	probably null	Het
Olfr767	T	A	10: 129,079,961	M1L	probably benign	Het
Olfr867	A	T	9: 20,055,365	S33T	probably benign	Het
Rab6b	T	C	9: 103,167,124	S172P	probably benign	Het
Ror1	T	A	4: 100,333,620	L58*	probably null	Het
Sec23b	A	G	2: 144,590,338	D756G	possibly damaging	Het
Slc24a2	T	A	4: 87,032,275	K428N	probably damaging	Het
Spen	C	A	4: 141,475,752	V1855L	probably benign	Het
Srgap1	G	A	10: 121,785,445	P1071S	possibly damaging	Het
Tns1	A	G	1: 73,941,969	C1079R	probably damaging	Het
Traf3ip1	G	T	1: 91,518,319		probably null	Het
Trmt2a	T	C	16: 18,249,709	F82S	probably damaging	Het
Xrn1	A	G	9: 96,039,737	D1401G	probably damaging	Het
Zfp352	T	A	4: 90,224,156	S178T	possibly damaging	Het
Zfp758	G	T	17: 22,375,759	E409*	probably null	Het

Other mutations in Gpr108

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01982:Gpr108	APN	17	57237877	missense	probably damaging	1.00
IGL02486:Gpr108	APN	17	57235977	missense	probably damaging	1.00
IGL02810:Gpr108	APN	17	57242742	missense	probably benign	0.39
IGL03233:Gpr108	APN	17	57245042	missense	probably benign	0.00
1mM(1):Gpr108	UTSW	17	57245352	unclassified	probably benign
IGL02799:Gpr108	UTSW	17	57237482	missense	probably damaging	1.00
PIT4468001:Gpr108	UTSW	17	57247563	missense	probably null	0.09
R0391:Gpr108	UTSW	17	57243101	missense	probably benign	0.02
R0469:Gpr108	UTSW	17	57235358	missense	possibly damaging	0.65
R0510:Gpr108	UTSW	17	57235358	missense	possibly damaging	0.65
R0613:Gpr108	UTSW	17	57238174	splice site	probably benign
R1141:Gpr108	UTSW	17	57237219	missense	probably damaging	1.00
R1748:Gpr108	UTSW	17	57236217	missense	probably damaging	0.99
R2000:Gpr108	UTSW	17	57236712	missense	probably benign	0.03
R3036:Gpr108	UTSW	17	57245323	missense	probably benign	0.01
R4087:Gpr108	UTSW	17	57237925	missense	probably damaging	1.00
R4089:Gpr108	UTSW	17	57237925	missense	probably damaging	1.00
R5071:Gpr108	UTSW	17	57235335	missense	probably damaging	1.00
R5566:Gpr108	UTSW	17	57236919	missense	probably damaging	1.00
R6889:Gpr108	UTSW	17	57236990	missense	probably damaging	1.00
R7352:Gpr108	UTSW	17	57236944	missense	probably damaging	1.00
R7587:Gpr108	UTSW	17	57236732	missense	probably damaging	1.00
R7642:Gpr108	UTSW	17	57236228	nonsense	probably null
R9574:Gpr108	UTSW	17	57238039	missense	probably damaging	1.00
Z1177:Gpr108	UTSW	17	57237316	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGCACTGAGACCAGCATTAGAGCC -3'
(R):5'- CGTATCATCGCCATTCTGCTGCAAG -3'

Sequencing Primer
(F):5'- ATTAGAGCCACCCATACCTTAC -3'
(R):5'- TGCAAGTAGCAGTGCCCTTC -3'

Posted On

2014-01-05