Mutagenetix > Incidental Mutation

Incidental Mutation 'R1124:Grm3'

95948

Institutional Source

Beutler Lab

Gene Symbol

Grm3

Ensembl Gene

ENSMUSG00000003974

Gene Name

glutamate receptor, metabotropic 3

Synonyms

mGluR3, mGlu3, 0710001G23Rik, Gprc1c

MMRRC Submission

039197-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1124 (G1)

Quality Score

165

Status

Not validated

Chromosome

Chromosomal Location

9535541-9775170 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 9620297 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Isoleucine at position 316 (V316I)

Ref Sequence

ENSEMBL: ENSMUSP00000004076 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000004076]

AlphaFold

Q9QYS2

Predicted Effect

probably benign
Transcript: ENSMUST00000004076
AA Change: V316I

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000004076
Gene: ENSMUSG00000003974
AA Change: V316I

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	67	473	4.8e-102	PFAM
Pfam:NCD3G	505	555	2.4e-14	PFAM
Pfam:7tm_3	588	825	4.7e-62	PFAM
low complexity region	849	861	N/A	INTRINSIC

Coding Region Coverage

1x: 98.8%
3x: 97.8%
10x: 94.7%
20x: 86.6%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a targeted mutation display normal morphology, clinical chemistry, hematology, and behavior. Mice homozygous for a knock-out allele exhibit altered neuroprotection. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 31 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3425401B19Rik	C	T	14: 32,384,039 (GRCm39)	R642Q	possibly damaging	Het
Aadacl2fm3	A	G	3: 59,772,639 (GRCm39)	T48A	probably benign	Het
Aadacl4fm1	A	G	4: 144,255,194 (GRCm39)	T205A	probably benign	Het
Aadacl4fm4	T	C	4: 144,396,845 (GRCm39)	S296G	probably benign	Het
Anxa10	C	T	8: 62,514,038 (GRCm39)		probably null	Het
Bcl11a	A	T	11: 24,113,928 (GRCm39)	M424L	probably damaging	Het
Bhlhe41	A	G	6: 145,809,456 (GRCm39)	S119P	probably damaging	Het
C2cd3	A	G	7: 100,071,888 (GRCm39)	D1033G	probably benign	Het
Ccdc174	T	C	6: 91,876,561 (GRCm39)	V466A	probably benign	Het
Dnaaf11	T	A	15: 66,310,264 (GRCm39)	T335S	possibly damaging	Het
Drd2	T	C	9: 49,306,940 (GRCm39)	Y9H	probably damaging	Het
Fbxw14	T	A	9: 109,105,236 (GRCm39)	I310F	possibly damaging	Het
Gys2	A	G	6: 142,391,739 (GRCm39)	Y508H	probably damaging	Het
Lrp1b	T	A	2: 40,765,063 (GRCm39)	D2921V	probably damaging	Het
Macrod2	A	G	2: 140,294,547 (GRCm39)	K71R	probably damaging	Het
Myh7	A	T	14: 55,211,327 (GRCm39)	V1614E	possibly damaging	Het
Nckap1	C	T	2: 80,348,286 (GRCm39)	S889N	probably benign	Het
Or8k23	T	A	2: 86,186,239 (GRCm39)	K162N	probably damaging	Het
Pirb	T	A	7: 3,722,731 (GRCm39)	N87I	probably benign	Het
Pmfbp1	A	G	8: 110,257,115 (GRCm39)		probably null	Het
Rasa4	A	G	5: 136,134,510 (GRCm39)	N627S	probably benign	Het
Spata31d1b	A	C	13: 59,864,468 (GRCm39)	M539L	probably benign	Het
Tbc1d23	A	T	16: 57,034,525 (GRCm39)		probably null	Het
Tnfrsf1b	A	T	4: 144,950,926 (GRCm39)	L229Q	probably benign	Het
Trip12	A	T	1: 84,714,758 (GRCm39)	V443E	probably damaging	Het
Ush2a	G	T	1: 188,485,733 (GRCm39)	V2948L	probably damaging	Het
Vmn1r204	A	T	13: 22,741,209 (GRCm39)	Y280F	possibly damaging	Het
Vmn2r115	ATCTTCT	ATCT	17: 23,578,962 (GRCm39)		probably benign	Het
Vmn2r93	G	T	17: 18,518,710 (GRCm39)	K56N	probably benign	Het
Xrn1	A	G	9: 95,885,918 (GRCm39)	I880V	probably benign	Het
Zfp106	C	T	2: 120,365,195 (GRCm39)	G404E	probably benign	Het

Other mutations in Grm3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00495:Grm3	APN	5	9,562,290 (GRCm39)	missense	probably benign
IGL01393:Grm3	APN	5	9,639,856 (GRCm39)	missense	probably benign	0.00
IGL01398:Grm3	APN	5	9,535,762 (GRCm39)	unclassified	probably benign
IGL01825:Grm3	APN	5	9,561,600 (GRCm39)	missense	probably damaging	1.00
IGL01966:Grm3	APN	5	9,561,486 (GRCm39)	missense	probably damaging	0.98
IGL02367:Grm3	APN	5	9,561,660 (GRCm39)	missense	probably damaging	1.00
IGL02526:Grm3	APN	5	9,639,847 (GRCm39)	missense	probably damaging	1.00
IGL02972:Grm3	APN	5	9,562,410 (GRCm39)	missense	probably damaging	1.00
IGL03356:Grm3	APN	5	9,562,206 (GRCm39)	missense	possibly damaging	0.89
BB002:Grm3	UTSW	5	9,639,880 (GRCm39)	missense	probably benign	0.09
BB012:Grm3	UTSW	5	9,639,880 (GRCm39)	missense	probably benign	0.09
R0032:Grm3	UTSW	5	9,561,452 (GRCm39)	splice site	probably null
R0032:Grm3	UTSW	5	9,561,452 (GRCm39)	splice site	probably null
R0389:Grm3	UTSW	5	9,554,794 (GRCm39)	missense	probably damaging	1.00
R0455:Grm3	UTSW	5	9,562,477 (GRCm39)	missense	probably benign
R0538:Grm3	UTSW	5	9,562,446 (GRCm39)	missense	possibly damaging	0.95
R0553:Grm3	UTSW	5	9,620,048 (GRCm39)	missense	probably benign	0.16
R1163:Grm3	UTSW	5	9,620,738 (GRCm39)	missense	probably benign	0.34
R1440:Grm3	UTSW	5	9,639,958 (GRCm39)	missense	probably benign
R1635:Grm3	UTSW	5	9,561,520 (GRCm39)	missense	probably damaging	1.00
R1734:Grm3	UTSW	5	9,639,742 (GRCm39)	missense	probably benign	0.00
R1895:Grm3	UTSW	5	9,562,123 (GRCm39)	missense	probably damaging	1.00
R1926:Grm3	UTSW	5	9,554,881 (GRCm39)	missense	probably damaging	0.98
R1940:Grm3	UTSW	5	9,561,682 (GRCm39)	missense	probably damaging	1.00
R1946:Grm3	UTSW	5	9,562,123 (GRCm39)	missense	probably damaging	1.00
R2004:Grm3	UTSW	5	9,639,793 (GRCm39)	missense	possibly damaging	0.57
R2005:Grm3	UTSW	5	9,639,793 (GRCm39)	missense	possibly damaging	0.57
R2006:Grm3	UTSW	5	9,639,793 (GRCm39)	missense	possibly damaging	0.57
R3116:Grm3	UTSW	5	9,620,752 (GRCm39)	missense	probably damaging	1.00
R4083:Grm3	UTSW	5	9,562,054 (GRCm39)	missense	probably benign
R4537:Grm3	UTSW	5	9,562,083 (GRCm39)	missense	probably benign	0.02
R4855:Grm3	UTSW	5	9,620,047 (GRCm39)	missense	probably damaging	0.98
R5060:Grm3	UTSW	5	9,620,167 (GRCm39)	missense	probably damaging	0.99
R5093:Grm3	UTSW	5	9,639,766 (GRCm39)	missense	probably benign	0.01
R5419:Grm3	UTSW	5	9,620,233 (GRCm39)	missense	probably damaging	1.00
R5525:Grm3	UTSW	5	9,554,872 (GRCm39)	missense	probably damaging	1.00
R5642:Grm3	UTSW	5	9,620,536 (GRCm39)	missense	probably benign	0.21
R5804:Grm3	UTSW	5	9,620,155 (GRCm39)	missense	probably benign	0.33
R5915:Grm3	UTSW	5	9,561,927 (GRCm39)	missense	probably damaging	1.00
R5966:Grm3	UTSW	5	9,561,930 (GRCm39)	missense	probably damaging	0.98
R6151:Grm3	UTSW	5	9,561,556 (GRCm39)	missense	probably damaging	1.00
R6419:Grm3	UTSW	5	9,620,201 (GRCm39)	missense	probably damaging	1.00
R7267:Grm3	UTSW	5	9,639,581 (GRCm39)	missense	probably benign	0.00
R7555:Grm3	UTSW	5	9,620,000 (GRCm39)	missense	probably benign	0.00
R7657:Grm3	UTSW	5	9,561,452 (GRCm39)	splice site	probably null
R7925:Grm3	UTSW	5	9,639,880 (GRCm39)	missense	probably benign	0.09
R8032:Grm3	UTSW	5	9,562,272 (GRCm39)	missense	probably benign	0.11
R8227:Grm3	UTSW	5	9,620,242 (GRCm39)	missense	possibly damaging	0.65
R8462:Grm3	UTSW	5	9,562,365 (GRCm39)	missense	probably benign
R8500:Grm3	UTSW	5	9,561,726 (GRCm39)	missense	probably benign	0.21
R8696:Grm3	UTSW	5	9,562,311 (GRCm39)	missense	probably damaging	1.00
R8807:Grm3	UTSW	5	9,561,499 (GRCm39)	missense	probably damaging	1.00
R8828:Grm3	UTSW	5	9,554,725 (GRCm39)	missense	probably benign	0.00
R8876:Grm3	UTSW	5	9,561,580 (GRCm39)	missense	probably damaging	1.00
R8896:Grm3	UTSW	5	9,562,483 (GRCm39)	missense	possibly damaging	0.91
R9035:Grm3	UTSW	5	9,620,464 (GRCm39)	missense	probably damaging	0.99
R9779:Grm3	UTSW	5	9,561,656 (GRCm39)	missense	possibly damaging	0.74
X0020:Grm3	UTSW	5	9,562,195 (GRCm39)	missense	probably damaging	1.00
X0025:Grm3	UTSW	5	9,535,790 (GRCm39)	missense	probably damaging	1.00
X0026:Grm3	UTSW	5	9,562,238 (GRCm39)	nonsense	probably null
Z1088:Grm3	UTSW	5	9,620,183 (GRCm39)	missense	probably damaging	0.97

Predicted Primers

PCR Primer
(F):5'- GCCCACTGCTTACCTGTGAAGTTG -3'
(R):5'- CTGCATCGCCACTGCTGAAAAG -3'

Sequencing Primer
(F):5'- CATTGCATCACAGAGCTTGG -3'
(R):5'- TCCTACGACAGCGTGATCC -3'

Posted On

2014-01-05